1 Setup

Set knitting options

# global knitting options for automatic saving of all plots as .png and .pdf. Also sets cache directory.
knitr::opts_chunk$set(
  dev = c("png", "pdf"), fig.keep = "all",
  dev.args = list(pdf = list(encoding = "WinAnsi", useDingbats = FALSE)),
  fig.path = file.path("fig_output/", paste0(gsub("\\.[Rr]md", "/", knitr::current_input()))),
  cache.path = file.path("cache/", paste0(gsub("\\.[Rr]md", "/", knitr::current_input())))
)

Load packages

library(dada2); packageVersion("dada2") # sequence filtering and sample inference with dada2
## [1] '1.16.0'
library(ShortRead); packageVersion("ShortRead") # package for loading, displaying, and handling sequences
## [1] '1.46.0'
library(tidyverse); packageVersion("tidyverse") # handy piping and data operations
## [1] '1.3.0'
library(rjson); packageVersion("rjson") # read json output from figaro
## [1] '0.2.20'
# source all relevant scripting files
# paths.R contains paths to local data and programs
source(file.path("scripts", "paths.R"))

Set directories

# Set up names of sub directories to stay organized
preprocess_path <- file.path(project_path_OM17, "01_preprocess")
    demultiplex_path <- file.path(preprocess_path, "demultiplexed")
        demultiplex_assigned_path <- file.path(demultiplex_path, "assigned")
    figaro_output_path <- file.path(preprocess_path, "Figaro_output")
filter_path <- file.path(project_path_OM17, "02_filter") 

Now we read in the names of the demultiplexed and assigned fastq files.

fnFs <- sort(list.files(demultiplex_assigned_path, pattern="_R1.fastq.gz", full.names = TRUE))
fnRs <- sort(list.files(demultiplex_assigned_path, pattern="_R2.fastq.gz", full.names = TRUE))

Count and display reads for input

# count forward reads
input_read_count_F <- countLines(demultiplex_assigned_path, pattern="_R1.fastq.gz")/4

# make forward read counts into tbl with sample name
input_read_count_F_tbl <- input_read_count_F %>% as_tibble(rownames = "file_name") %>% rename(reads_F = value) %>% mutate(sample_id = sapply(strsplit(
file_name, "_R1"), `[`, 1))

# repeat for reverse
input_read_count_R <- countLines(demultiplex_assigned_path, pattern="_R2.fastq.gz")/4

input_read_count_R_tbl <- input_read_count_R %>% as_tibble(rownames = "file_name") %>% rename(reads_R = value) %>% mutate(sample_id = sapply(strsplit(
file_name, "_R2"), `[`, 1))

# Join forward and reverse together into one tbl
input_read_count_FR <- input_read_count_F_tbl %>% select(-file_name) %>% left_join(input_read_count_R_tbl %>% select(-file_name), by = "sample_id") %>% select(sample_id, everything())

# print. reads counts are same in forward and reverse, which is what we want.
input_read_count_FR

Plot read counts

plot_read_count_input <- input_read_count_FR %>% ggplot(aes(
  x = fct_reorder(sample_id, desc(reads_F)),
  y = reads_F
)) +
  geom_bar(stat = "identity") +
  scale_y_continuous(name = "Forward reads") +
  scale_x_discrete(name = "Sample ID") +
  theme_classic(base_size = 9)+
  theme(
    axis.text.x = element_text(angle = 90, vjust = .5, hjust = 1),
    legend.position = "bottom"
  )

plot_read_count_input

Extract sample names for later use

# Extract sample names, assuming filenames have format: SAMPLENAME_XXX.fastq
sample.namesF.dots <- sapply(strsplit(basename(fnFs), "_R1"), `[`, 1)

sample.namesR.dots <- sapply(strsplit(basename(fnRs), "_R2"), `[`, 1)

# print first few names
sample.namesF.dots %>% head()
## [1] "04.RNA1"  "04.RNA2"  "104.1"    "104.2"    "104.3"    "104.RNA1"
sample.namesR.dots %>% head()
## [1] "04.RNA1"  "04.RNA2"  "104.1"    "104.2"    "104.3"    "104.RNA1"

2 Check primer sequences

FWD <- "CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA" # 515-Y-M13
REV <- "CCGYCAATTYMTTTRAGTTT" # 926R

(FWD_length <- str_length(FWD))
## [1] 40
(REV_length <- str_length(REV))
## [1] 20

To ensure we have the right primers, and the correct orientation of the primers on the reads, we will verify the presence and orientation of these primers in the data.

allOrients <- function(primer) {
    # Create all orientations of the input sequence
    require(Biostrings)
    dna <- DNAString(primer)  # The Biostrings works w/ DNAString objects rather than character vectors
    orients <- c(Forward = dna, Complement = complement(dna), Reverse = reverse(dna), 
        RevComp = reverseComplement(dna))
    return(sapply(orients, toString))  # Convert back to character vector
}
FWD.orients <- allOrients(FWD)
REV.orients <- allOrients(REV)
FWD.orients
##                                    Forward 
## "CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA" 
##                                 Complement 
## "GGCATTTTGCTGCCGGTCAGGCACRGTCGKCGGCGCCATT" 
##                                    Reverse 
## "AATGGCGCCGMCGACYGTGCCTGACCGGCAGCAAAATGCC" 
##                                    RevComp 
## "TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG"

We are now ready to count the number of times the primers appear in the forward and reverse read, while considering all possible primer orientations. Identifying and counting the primers on one set of paired end FASTQ files is sufficient, assuming all the files were created using the same library preparation, so we’ll just process the first sample.

primerHits <- function(primer, fn) { # Counts number of reads in which the primer is found
    nhits <- vcountPattern(primer, sread(readFastq(fn)), fixed = FALSE)
    return(sum(nhits > 0))
}

rbind(FWD.ForwardReads = sapply(FWD.orients, primerHits, fn=fnFs[[1]]),
      FWD.ReverseReads = sapply(FWD.orients, primerHits, fn=fnRs[[1]]),
      REV.ForwardReads = sapply(REV.orients, primerHits, fn=fnFs[[1]]),
      REV.ReverseReads = sapply(REV.orients, primerHits, fn=fnRs[[1]]))
##                  Forward Complement Reverse RevComp
## FWD.ForwardReads    7137          0       0       0
## FWD.ReverseReads       0          0       0     551
## REV.ForwardReads       1          0       0      24
## REV.ReverseReads    5988          0       0       7

As expected, the FWD primer is found in the forward reads in its forward orientation, and in the reverse reads in its reverse-complement orientation. Similarly, the REV primer is found with its expected orientations. We will remove the primers later when we truncate lower quality reads. So now let’s inspect read quality profiles.

3 Inspect read quality profiles

It’s important to get a feel for the quality of the data that we are using. To do this, we will plot the quality of some of the samples.

From the dada2 tutorial: >In gray-scale is a heat map of the frequency of each quality score at each base position. The median quality score at each position is shown by the green line, and the quartiles of the quality score distribution by the orange lines. The red line shows the scaled proportion of reads that extend to at least that position (this is more useful for other sequencing technologies, as Illumina reads are typically all the same lenghth, hence the flat red line).

# Forward reads
# If the number of samples is 20 or less, plot them all, otherwise, just plot 20 randomly selected samples
if( length(fnFs) <= 20) {
  plotQualityProfile(fnFs)
} else {
  rand_samples <- sample(size = 20, 1:length(fnFs)) # grab 20 random samples to plot
  plotQualityProfile(fnFs[rand_samples])
}

# Reverse reads
# If the number of samples is 20 or less, plot them all, otherwise, just plot 20 randomly selected samples
if( length(fnRs) <= 20) {
  plotQualityProfile(fnRs)
} else {
  rand_samples <- sample(size = 20, 1:length(fnRs)) # grab 20 random samples to plot
  plotQualityProfile(fnRs[rand_samples])
}

4 Filter parameter optimization with Figaro

Currently, Figaro (v1.1.1) only accepts files with Illumina naming convention, so we need to rename the files.

# get original file names
R1_names <- gsub(demultiplex_assigned_path , "", list.files(demultiplex_assigned_path , pattern="R1", full.names = TRUE))

# remove suffix
R1_names <- gsub("_R1.fastq.gz", "", R1_names)

# remove dots (Figaro v1.1.1 will throw an error if there dots in the sample name)
R1_names <- R1_names %>% str_replace_all("\\.", "")

# I'm not sure this is necessary, but anyway, I'm adding incrementing Sample digits after S in Illumina style
R1_names_incremented <- vector("character", length(R1_names))
for (i in seq_along(R1_names_incremented )) {
  R1_names_incremented[[i]] <- paste0(R1_names[[i]], "_S", i)
}

# put names back together, appending Illumina naming convention, which is the format for later processing with Figaro
R1_names_incremented <- paste0(R1_names_incremented, "_L001_R1_001.fastq.gz")

# Now, rename files
file.rename(from = list.files(demultiplex_assigned_path , pattern="R1", full.names = TRUE), to = file.path(demultiplex_assigned_path, R1_names_incremented))
##   [1] TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE
##  [16] TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE
##  [31] TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE
##  [46] TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE
##  [61] TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE
##  [76] TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE
##  [91] TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE
## [106] TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE
## [121] TRUE TRUE TRUE TRUE TRUE TRUE TRUE
# Repeat the above with the reverse reads

R2_names <- gsub(demultiplex_assigned_path , "", list.files(demultiplex_assigned_path , pattern="R2", full.names = TRUE))

R2_names <- gsub("_R2.fastq.gz", "", R2_names)

R2_names <- R2_names %>% str_replace_all("\\.", "")

R2_names_incremented <- vector("character", length(R2_names))
for (i in seq_along(R2_names_incremented )) {
  R2_names_incremented[[i]] <- paste0(R2_names[[i]], "_S", i)
}

R2_names_incremented <- paste0(R2_names_incremented, "_L001_R2_001.fastq.gz")

file.rename(from = list.files(demultiplex_assigned_path , pattern="R2", full.names = TRUE), to = file.path(demultiplex_assigned_path, R2_names_incremented))
##   [1] TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE
##  [16] TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE
##  [31] TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE
##  [46] TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE
##  [61] TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE
##  [76] TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE
##  [91] TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE
## [106] TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE
## [121] TRUE TRUE TRUE TRUE TRUE TRUE TRUE

Check that renaming worked

R1_names_incremented %>% head()
## [1] "/04RNA1_S1_L001_R1_001.fastq.gz"  "/04RNA2_S2_L001_R1_001.fastq.gz" 
## [3] "/1041_S3_L001_R1_001.fastq.gz"    "/1042_S4_L001_R1_001.fastq.gz"   
## [5] "/1043_S5_L001_R1_001.fastq.gz"    "/104RNA1_S6_L001_R1_001.fastq.gz"

Create a directory for Figaro output

# Create directory to hold the output from figaro
if (!dir.exists(figaro_output_path)) dir.create(figaro_output_path)

I couldn’t get figaro (v1.1.1) to run by system2() in R due to a shell permission denied error, but it worked on the command line. This chunk generates the exact text I entered in the command line after changing to the figaro directory. I then just read in the figaro output JSON and continue from there.

# text to change directory in terminal to figaro 
paste("cd", figaro_path) %>% str_remove("/figaro.py") %>% cat()
## cd /Users/melo.d/opt/figaro
# The length of the amplified sequence target not including primers.
# Kraus et al. (in review) says the 515-Y M13 and 926R primer primer set produce gene fragments of ~400 bp 
# primers total 60 bp
# sequences in 2X250 bp
# I originally tried 340 bp for figaro, but that resulted in low merged read percentage
# EK did not truncate left at all in her original processing
# Thus, I increased to 400
figaro_amplicon_length <- 400

figaro_min_overlap <- 20

figaro_output_file_name <- "Figaro_params_OM17.json"

flags_figaro <- paste("--ampliconLength", figaro_amplicon_length, "--forwardPrimerLength", FWD_length, "--reversePrimerLength", REV_length, "--outputFileName", figaro_output_file_name, "--inputDirectory", demultiplex_assigned_path, "--outputDirectory", figaro_output_path, "--minimumOverlap", figaro_min_overlap)

paste("python3", "figaro.py", flags_figaro) %>% cat()
## python3 figaro.py --ampliconLength 400 --forwardPrimerLength 40 --reversePrimerLength 20 --outputFileName Figaro_params_OM17.json --inputDirectory /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/01_preprocess/demultiplexed/assigned --outputDirectory /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/01_preprocess/Figaro_output --minimumOverlap 20

Read in Figaro JSON output, top 5 suggested trim parameters.

# Give the input file name to the function.
figaro_result <- fromJSON(file = file.path(figaro_output_path, figaro_output_file_name))

figaro_result[1:5] %>% paste(collapse = '\n') %>% cat()
## list(trimPosition = c(236, 244), maxExpectedError = c(2, 4), readRetentionPercent = 87.41, score = 77.4082823910813)
## list(trimPosition = c(235, 245), maxExpectedError = c(2, 4), readRetentionPercent = 87.33, score = 77.3272346332589)
## list(trimPosition = c(234, 246), maxExpectedError = c(2, 4), readRetentionPercent = 87.28, score = 77.2832619135893)
## list(trimPosition = c(233, 247), maxExpectedError = c(2, 4), readRetentionPercent = 87.19, score = 77.1918676334917)
## list(trimPosition = c(232, 248), maxExpectedError = c(2, 4), readRetentionPercent = 87.12, score = 77.1177175571861)
# Save and print Figaro-suggested parameters for dada2::filterAndTrim 

(truncLen_best_figaro <- figaro_result[[1]]$trimPosition)
## [1] 236 244
(maxEE_best_figaro <- figaro_result[[1]]$maxExpectedError)
## [1] 2 4

5 Filter and trim for quality

5.1 Organize file paths and directories

# set file path for quality-filtered read
filter_path <- file.path(project_path_OM17, "02_filter") 

# Put filtered reads into separate sub-directories for big data workflow

if (!dir.exists(filter_path)) dir.create(filter_path)
    subF_path <- file.path(filter_path, "preprocessed_F") 
    subR_path <- file.path(filter_path, "preprocessed_R") 
if (!dir.exists(subF_path)) dir.create(subF_path)
if (!dir.exists(subR_path)) dir.create(subR_path)
    
# Move R1 and R2 from trimmed to separate forward/reverse sub-directories
# fnFs.Q <- file.path(subF_path,  basename(fnFs)) 
# fnRs.Q <- file.path(subR_path,  basename(fnRs))

fnFs.Q <- file.path(subF_path,  basename(list.files(demultiplex_assigned_path , pattern="R1", full.names = TRUE))) 
fnRs.Q <- file.path(subR_path,  basename(list.files(demultiplex_assigned_path , pattern="R2", full.names = TRUE)))

file.rename(from = list.files(demultiplex_assigned_path , pattern="R1", full.names = TRUE), to = fnFs.Q)
##   [1] TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE
##  [16] TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE
##  [31] TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE
##  [46] TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE
##  [61] TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE
##  [76] TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE
##  [91] TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE
## [106] TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE
## [121] TRUE TRUE TRUE TRUE TRUE TRUE TRUE
file.rename(from = list.files(demultiplex_assigned_path , pattern="R2", full.names = TRUE), to = fnRs.Q)
##   [1] TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE
##  [16] TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE
##  [31] TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE
##  [46] TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE
##  [61] TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE
##  [76] TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE
##  [91] TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE
## [106] TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE TRUE
## [121] TRUE TRUE TRUE TRUE TRUE TRUE TRUE
# File parsing; create file names and make sure that forward and reverse files match
filtpathF <- file.path(subF_path, "filtered") # files go into preprocessed_F/filtered/
filtpathR <- file.path(subR_path, "filtered") # ...
fastqFs <- sort(list.files(subF_path, pattern="fastq.gz"))
fastqRs <- sort(list.files(subR_path, pattern="fastq.gz"))
if(length(fastqFs) != length(fastqRs)) stop("Forward and reverse files do not match.")

Before chosing sequence variants, we want to trim reads where their quality scores begin to drop (the truncLen and truncQ values) and remove any low-quality reads that are left over after we have finished trimming (the maxEE value).

5.2 Filter and trim

# also trimming lengths of primers in this step with trimLeft
filt_out <- filterAndTrim(fwd = file.path(subF_path, fastqFs), filt = file.path(filtpathF, fastqFs), rev = file.path(subR_path, fastqRs), filt.rev = file.path(filtpathR, fastqRs), trimLeft = c(FWD_length, REV_length), truncLen = truncLen_best_figaro, maxEE = maxEE_best_figaro, truncQ = 2, maxN = 0, rm.phix = TRUE, compress = TRUE, verbose = TRUE, multithread = TRUE)

# look at how many reads were kept
head(filt_out)
##                                 reads.in reads.out
## 04RNA1_S1_L001_R1_001.fastq.gz      7756      5911
## 04RNA2_S2_L001_R1_001.fastq.gz      4033      2848
## 1041_S3_L001_R1_001.fastq.gz       10611      9515
## 1042_S4_L001_R1_001.fastq.gz       17059     15301
## 1043_S5_L001_R1_001.fastq.gz       18852     16701
## 104RNA1_S6_L001_R1_001.fastq.gz     5320      4721
# summary of samples in filt_out by percentage
filt_out %>% 
  data.frame() %>% 
  mutate(Samples = rownames(.),
         percent_kept = 100*(reads.out/reads.in)) %>%
  select(Samples, everything()) %>%
  summarise(min_remaining = paste0(round(min(percent_kept), 2), "%"), 
            median_remaining = paste0(round(median(percent_kept), 2), "%"),
            mean_remaining = paste0(round(mean(percent_kept), 2), "%"), 
            max_remaining = paste0(round(max(percent_kept), 2), "%"))

5.3 Check if primers were removed by filterAndTrim

rbind(FWD.ForwardReads = sapply(FWD.orients, primerHits, fn=filtpathF[[1]]),
      FWD.ReverseReads = sapply(FWD.orients, primerHits, fn=filtpathR[[1]]),
      REV.ForwardReads = sapply(REV.orients, primerHits, fn=filtpathF[[1]]),
      REV.ReverseReads = sapply(REV.orients, primerHits, fn=filtpathR[[1]]))
##                  Forward Complement Reverse RevComp
## FWD.ForwardReads       1          0       0    1473
## FWD.ReverseReads       1          0       0   14134
## REV.ForwardReads      32          0       0    2622
## REV.ReverseReads      36          0       0     237

The vast majority of primer hits were removed during filtering, but a considerable amount remain. These need to be removed. We will do so with cutadapt.

The primerHits function only scores a hit if the full primer sequence is found in the read. That would be 40 bases for the FWD primer or 20 for the REV primer in this case. The fact that so many full primer sequences remain in the reads after filtering suggest that they are internal (i.e. regular adapters as opposed to anchored adapters in the cutadapt lingo). So, let’s use regular adapters. However, to preserve the possibility that some biological reads happen by chance to be the same as the primer sequences, let’s increase the minimum overlap in cutadapt from the default of 3 bases to a somewhat more restrictive 5 bases for the REV primers and 7 bases for the FWD primer (because it is longer and thus more likely to have). Since reverse complements are found, let’s also enable reverse complements

6 Remove primers with cutadapt

6.1 Set up directories for cutadapt

# create directories to put the files trimmed by cutadapt
filt_and_cut_path_F <- file.path(subF_path, "filtered_and_cut")
filt_and_cut_path_R <- file.path(subR_path, "filtered_and_cut") 

# Create directory to hold the output from cutadapt
if (!dir.exists(filt_and_cut_path_F)) dir.create(filt_and_cut_path_F)
if (!dir.exists(filt_and_cut_path_R)) dir.create(filt_and_cut_path_R)

filtfastqFs <- sort(file.path(filtpathF, list.files(filtpathF, pattern="fastq.gz")))

fnFs.cut <- file.path(filt_and_cut_path_F, basename(filtfastqFs))

filtfastqRs <- sort(file.path(filtpathR, list.files(filtpathR, pattern="fastq.gz")))

fnRs.cut <- file.path(filt_and_cut_path_R, basename(filtfastqRs))

6.2 Run cutadapt

# Save the reverse complements of the primers to variables
FWD.RC <- dada2:::rc(FWD)
REV.RC <- dada2:::rc(REV)


# minimum length to output reads for cutadapt
minimum_length_cutadapt <- 50

# minimum overlap for "adapter" (barcode) to be detected by cutadapt
# minimum_overlap_cutadapt_FWD <- 5
# minimum_overlap_cutadapt_REV <- 7
minimum_overlap_cutadapt <- 5

##  Create the cutadapt flags ##
# Trim FWD and the reverse-complement of REV off of R1 (forward reads)
R1.flags <- paste("-g", FWD, "-a", REV.RC)

# Trim REV and the reverse-complement of FWD off of R2 (reverse reads)
R2.flags <- paste("-G", REV, "-A", FWD.RC)


## Run Cutadapt
# default error rate of 10% applied
# https://cutadapt.readthedocs.io/en/stable/guide.html#error-tolerance
# I increased the minimum overlap from the default of 3 to 5 to reduce random matches
# https://cutadapt.readthedocs.io/en/stable/guide.html#minimum-overlap-reducing-random-matches

 # -n 2 required to remove FWD and REV from reads
cutadapt_messages_2_list <- vector("list", length(filtfastqFs)) # set cutadapt message output list
for (i in seq_along(filtfastqFs)) {
     cutadapt_messages_2_list[[i]] <- system2(cutadapt_path, args = c(R1.flags, R2.flags, "--overlap", minimum_overlap_cutadapt, "--minimum-length", minimum_length_cutadapt, "-n", 2, "-o", fnFs.cut[i], "-p", fnRs.cut[i], filtfastqFs[i], filtfastqRs[i]), stdout = TRUE, stderr = TRUE) # input files
}

cutadapt_messages_2_list # print output text
## [[1]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/04RNA1_S1_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/04RNA1_S1_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/04RNA1_S1_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/04RNA1_S1_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##   [4] "Finished in 0.65 s (111 us/read; 0.54 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##   [8] "Total read pairs processed:              5,911"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##   [9] "  Read 1 with adapter:                      29 (0.5%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [10] "  Read 2 with adapter:                     626 (10.6%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [11] "Pairs written (passing filters):         5,910 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [13] "Total basepairs processed:     2,482,620 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [14] "  Read 1:     1,158,556 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [15] "  Read 2:     1,324,064 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [16] "Total written (filtered):      2,440,316 bp (98.3%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [17] "  Read 1:     1,157,062 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [18] "  Read 2:     1,283,254 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 4 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [29] "7\t1\t0.4\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [30] "11\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [31] "22\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [32] "63\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [34] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [35] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [36] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [37] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 25 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [39] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [40] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [41] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [42] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [43] "  A: 4.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [44] "  C: 4.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [45] "  G: 84.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [46] "  T: 8.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [47] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [48] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [49] "    The adapter is preceded by \"G\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [50] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [51] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [52] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [53] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [54] "7\t1\t0.4\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [55] "8\t1\t0.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [56] "18\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [57] "36\t14\t0.0\t2\t14"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [58] "62\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [59] "74\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [60] "82\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [61] "90\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [62] "118\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [63] "140\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [64] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [65] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [66] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [67] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [68] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [69] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [70] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [71] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [72] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 626 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [73] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [74] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [75] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [76] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [77] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [78] "  A: 95.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [79] "  C: 3.4%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [80] "  G: 0.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [81] "  T: 1.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [82] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [83] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [84] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [85] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [86] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [87] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [88] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [89] "5\t2\t5.8\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [90] "6\t5\t1.4\t0\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [91] "7\t1\t0.4\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [92] "8\t10\t0.1\t0\t10"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [93] "10\t4\t0.0\t1\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [94] "12\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [95] "13\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [96] "14\t19\t0.0\t1\t17 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [97] "15\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [98] "16\t15\t0.0\t1\t14 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [99] "17\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [100] "18\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [101] "19\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [102] "20\t4\t0.0\t2\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [103] "21\t7\t0.0\t2\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [104] "22\t11\t0.0\t2\t11"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [105] "23\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [106] "25\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [107] "26\t2\t0.0\t2\t0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [108] "27\t24\t0.0\t2\t17 6 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [109] "28\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [110] "29\t2\t0.0\t2\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [111] "30\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [112] "31\t2\t0.0\t3\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [113] "32\t8\t0.0\t3\t7 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [114] "33\t10\t0.0\t3\t8 1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [115] "34\t6\t0.0\t3\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [116] "35\t7\t0.0\t3\t6 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [117] "36\t3\t0.0\t3\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [118] "38\t3\t0.0\t3\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [119] "39\t3\t0.0\t3\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [120] "40\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [121] "41\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [122] "42\t15\t0.0\t4\t12 1 0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [123] "43\t6\t0.0\t4\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [124] "44\t5\t0.0\t4\t3 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [125] "45\t4\t0.0\t4\t2 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [126] "46\t9\t0.0\t4\t8 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [127] "47\t4\t0.0\t4\t3 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [128] "48\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [129] "50\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [130] "51\t13\t0.0\t4\t10 1 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [131] "52\t24\t0.0\t4\t21 1 1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [132] "53\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [133] "54\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [134] "55\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [135] "56\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [136] "57\t6\t0.0\t4\t3 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [137] "58\t17\t0.0\t4\t15 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [138] "59\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [139] "60\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [140] "61\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [141] "62\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [142] "63\t9\t0.0\t4\t9"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [143] "64\t24\t0.0\t4\t23 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [144] "65\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [145] "66\t7\t0.0\t4\t6 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [146] "67\t7\t0.0\t4\t5 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [147] "68\t8\t0.0\t4\t7 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [148] "69\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [149] "70\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [150] "71\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [151] "72\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [152] "73\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [153] "74\t4\t0.0\t4\t3 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [154] "75\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [155] "76\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [156] "77\t9\t0.0\t4\t9"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [157] "78\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [158] "79\t17\t0.0\t4\t14 2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [159] "80\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [160] "81\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [161] "83\t5\t0.0\t4\t3 1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [162] "84\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [163] "85\t12\t0.0\t4\t11 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [164] "86\t11\t0.0\t4\t9 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [165] "87\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [166] "88\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [167] "89\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [168] "90\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [169] "91\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [170] "92\t9\t0.0\t4\t9"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [171] "93\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [172] "94\t16\t0.0\t4\t12 3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [173] "95\t7\t0.0\t4\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [174] "96\t9\t0.0\t4\t8 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [175] "98\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [176] "99\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [177] "100\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [178] "101\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [179] "102\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [180] "103\t6\t0.0\t4\t5 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [181] "104\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [182] "105\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [183] "106\t6\t0.0\t4\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [184] "107\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [185] "108\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [186] "109\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [187] "110\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [188] "111\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [189] "112\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [190] "113\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [191] "114\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [192] "115\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [193] "116\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [194] "117\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [195] "118\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [196] "119\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [197] "121\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [198] "124\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [199] "125\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [200] "126\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [201] "128\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [202] "129\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [203] "132\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [204] "133\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [205] "136\t2\t0.0\t4\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [206] "137\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [207] "140\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [208] "142\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [209] "143\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [210] "144\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [211] "145\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [212] "146\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [213] "147\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [214] "148\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [215] "151\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [216] "152\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [217] "155\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [218] "159\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [219] "162\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [220] "165\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [221] "168\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [222] "173\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [223] "175\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [224] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [225] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [226] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [227] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [228] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## 
## [[2]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/04RNA2_S2_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/04RNA2_S2_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/04RNA2_S2_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/04RNA2_S2_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##   [4] "Finished in 0.32 s (114 us/read; 0.53 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##   [8] "Total read pairs processed:              2,848"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##   [9] "  Read 1 with adapter:                      10 (0.4%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [10] "  Read 2 with adapter:                     209 (7.3%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [11] "Pairs written (passing filters):         2,847 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [13] "Total basepairs processed:     1,196,160 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [14] "  Read 1:       558,208 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [15] "  Read 2:       637,952 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [16] "Total written (filtered):      1,181,668 bp (98.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [17] "  Read 1:       557,498 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [18] "  Read 2:       624,170 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 3 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [29] "6\t1\t0.7\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [30] "7\t1\t0.2\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [31] "31\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [32] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [34] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [36] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 7 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [38] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [39] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [40] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [41] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [42] "  A: 28.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [43] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [44] "  G: 71.4%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [45] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [46] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [47] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [48] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [49] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [50] "36\t4\t0.0\t2\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [51] "90\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [52] "118\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [53] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [54] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [55] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [56] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [57] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [58] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [59] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [60] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [61] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 209 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [62] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [63] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [64] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [65] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [66] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [67] "  A: 93.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [68] "  C: 5.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [69] "  G: 0.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [70] "  T: 0.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [71] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [72] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [73] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [74] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [75] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [76] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [77] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [78] "5\t3\t2.8\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [79] "6\t1\t0.7\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [80] "8\t3\t0.0\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [81] "9\t1\t0.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [82] "10\t3\t0.0\t1\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [83] "11\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [84] "12\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [85] "14\t6\t0.0\t1\t4 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [86] "15\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [87] "16\t4\t0.0\t1\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [88] "17\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [89] "18\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [90] "21\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [91] "22\t3\t0.0\t2\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [92] "23\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [93] "24\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [94] "26\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [95] "27\t4\t0.0\t2\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [96] "29\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [97] "30\t3\t0.0\t3\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [98] "33\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [99] "34\t2\t0.0\t3\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [100] "35\t3\t0.0\t3\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [101] "36\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [102] "38\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [103] "40\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [104] "41\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [105] "42\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [106] "43\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [107] "44\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [108] "45\t5\t0.0\t4\t3 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [109] "46\t4\t0.0\t4\t3 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [110] "47\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [111] "50\t1\t0.0\t4\t0 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [112] "51\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [113] "52\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [114] "53\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [115] "54\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [116] "55\t2\t0.0\t4\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [117] "57\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [118] "58\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [119] "59\t6\t0.0\t4\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [120] "60\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [121] "62\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [122] "63\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [123] "64\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [124] "65\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [125] "67\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [126] "68\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [127] "69\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [128] "70\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [129] "72\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [130] "73\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [131] "74\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [132] "75\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [133] "76\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [134] "77\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [135] "79\t6\t0.0\t4\t4 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [136] "84\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [137] "85\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [138] "86\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [139] "87\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [140] "90\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [141] "92\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [142] "93\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [143] "94\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [144] "96\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [145] "99\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [146] "102\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [147] "103\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [148] "104\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [149] "105\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [150] "108\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [151] "111\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [152] "113\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [153] "114\t1\t0.0\t4\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [154] "115\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [155] "116\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [156] "117\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [157] "118\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [158] "120\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [159] "121\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [160] "123\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [161] "127\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [162] "128\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [163] "132\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [164] "134\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [165] "138\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [166] "142\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [167] "146\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [168] "154\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [169] "157\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [170] "163\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [171] "170\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [172] "183\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [173] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [174] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [175] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [176] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [177] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## 
## [[3]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/1041_S3_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/1041_S3_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/1041_S3_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/1041_S3_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##   [4] "Finished in 1.13 s (118 us/read; 0.51 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [8] "Total read pairs processed:              9,515"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##   [9] "  Read 1 with adapter:                      21 (0.2%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [10] "  Read 2 with adapter:                     136 (1.4%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [11] "Pairs written (passing filters):         9,515 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [13] "Total basepairs processed:     3,996,300 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [14] "  Read 1:     1,864,940 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [15] "  Read 2:     2,131,360 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [16] "Total written (filtered):      3,986,644 bp (99.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [17] "  Read 1:     1,863,964 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [18] "  Read 2:     2,122,680 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 6 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [29] "5\t1\t9.3\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [30] "7\t2\t0.6\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [31] "18\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [32] "23\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [34] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [35] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [36] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [37] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 15 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [39] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [40] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [41] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [42] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [43] "  A: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [44] "  C: 33.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [45] "  G: 66.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [46] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [47] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [48] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [49] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [50] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [51] "27\t4\t0.0\t2\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [52] "66\t10\t0.0\t2\t9 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [53] "125\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [54] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [55] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [56] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [57] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [58] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [59] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [60] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [61] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [62] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 136 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [63] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [64] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [65] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [66] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [67] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [68] "  A: 66.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [69] "  C: 22.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [70] "  G: 7.4%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [71] "  T: 3.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [72] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [73] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [74] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [75] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [76] "5\t9\t9.3\t0\t9"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [77] "6\t1\t2.3\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [78] "7\t3\t0.6\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [79] "8\t1\t0.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [80] "14\t2\t0.0\t1\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [81] "15\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [82] "16\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [83] "17\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [84] "18\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [85] "19\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [86] "20\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [87] "21\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [88] "22\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [89] "23\t3\t0.0\t2\t1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [90] "27\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [91] "28\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [92] "30\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [93] "31\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [94] "32\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [95] "33\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [96] "35\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [97] "37\t3\t0.0\t3\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [98] "38\t2\t0.0\t3\t1 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [99] "40\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [100] "41\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [101] "42\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [102] "43\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [103] "46\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [104] "47\t3\t0.0\t4\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [105] "51\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [106] "52\t2\t0.0\t4\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [107] "55\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [108] "56\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [109] "57\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [110] "58\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [111] "59\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [112] "60\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [113] "63\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [114] "66\t2\t0.0\t4\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [115] "67\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [116] "68\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [117] "71\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [118] "72\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [119] "73\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [120] "74\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [121] "75\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [122] "77\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [123] "79\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [124] "83\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [125] "84\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [126] "86\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [127] "87\t3\t0.0\t4\t1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [128] "89\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [129] "90\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [130] "93\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [131] "94\t12\t0.0\t4\t10 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [132] "95\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [133] "97\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [134] "98\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [135] "99\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [136] "105\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [137] "107\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [138] "108\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [139] "109\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [140] "113\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [141] "114\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [142] "118\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [143] "121\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [144] "128\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [145] "130\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [146] "131\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [147] "133\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [148] "134\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [149] "142\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [150] "147\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [151] "150\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [152] "153\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [153] "157\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [154] "163\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [155] "166\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## 
## [[4]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/1042_S4_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/1042_S4_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/1042_S4_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/1042_S4_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##   [4] "Finished in 1.82 s (119 us/read; 0.50 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [8] "Total read pairs processed:             15,301"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##   [9] "  Read 1 with adapter:                      26 (0.2%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [10] "  Read 2 with adapter:                     166 (1.1%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [11] "Pairs written (passing filters):        15,301 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [13] "Total basepairs processed:     6,426,420 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [14] "  Read 1:     2,998,996 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [15] "  Read 2:     3,427,424 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [16] "Total written (filtered):      6,414,209 bp (99.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [17] "  Read 1:     2,997,392 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [18] "  Read 2:     3,416,817 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [29] "13\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [30] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [32] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [34] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 25 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [36] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [37] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [39] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [40] "  A: 4.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [41] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [42] "  G: 96.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [43] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [44] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [45] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [46] "    The adapter is preceded by \"G\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [47] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [48] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [49] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [50] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [51] "6\t1\t3.7\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [52] "66\t23\t0.0\t2\t23"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [53] "67\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [54] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [55] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [56] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [57] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [58] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [59] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [60] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [61] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [62] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 166 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [63] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [64] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [65] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [66] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [67] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [68] "  A: 66.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [69] "  C: 26.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [70] "  G: 3.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [71] "  T: 3.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [72] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [73] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [74] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [75] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [76] "5\t13\t14.9\t0\t13"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [77] "6\t2\t3.7\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [78] "7\t1\t0.9\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [79] "8\t8\t0.2\t0\t8"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [80] "10\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [81] "11\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [82] "12\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [83] "15\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [84] "16\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [85] "20\t4\t0.0\t2\t3 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [86] "21\t2\t0.0\t2\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [87] "22\t2\t0.0\t2\t0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [88] "23\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [89] "24\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [90] "25\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [91] "27\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [92] "28\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [93] "29\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [94] "33\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [95] "35\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [96] "36\t5\t0.0\t3\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [97] "38\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [98] "40\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [99] "41\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [100] "42\t3\t0.0\t4\t0 0 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [101] "43\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [102] "46\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [103] "47\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [104] "50\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [105] "51\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [106] "52\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [107] "55\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [108] "61\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [109] "62\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [110] "63\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [111] "70\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [112] "72\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [113] "73\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [114] "74\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [115] "76\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [116] "81\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [117] "82\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [118] "83\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [119] "84\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [120] "85\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [121] "86\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [122] "87\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [123] "88\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [124] "89\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [125] "90\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [126] "92\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [127] "93\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [128] "94\t25\t0.0\t4\t20 5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [129] "98\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [130] "99\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [131] "100\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [132] "103\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [133] "104\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [134] "105\t7\t0.0\t4\t6 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [135] "108\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [136] "109\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [137] "113\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [138] "114\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [139] "115\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [140] "116\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [141] "125\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [142] "133\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [143] "134\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [144] "135\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [145] "139\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [146] "143\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [147] "147\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [148] "148\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [149] "170\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [150] "171\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [151] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [152] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [153] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [154] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [155] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## 
## [[5]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/1043_S5_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/1043_S5_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/1043_S5_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/1043_S5_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##   [4] "Finished in 1.98 s (119 us/read; 0.51 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [8] "Total read pairs processed:             16,701"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##   [9] "  Read 1 with adapter:                      22 (0.1%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [10] "  Read 2 with adapter:                     153 (0.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [11] "Pairs written (passing filters):        16,700 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [13] "Total basepairs processed:     7,014,420 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [14] "  Read 1:     3,273,396 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [15] "  Read 2:     3,741,024 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [16] "Total written (filtered):      7,004,720 bp (99.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [17] "  Read 1:     3,272,220 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [18] "  Read 2:     3,732,500 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 2 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [29] "11\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [30] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [32] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [34] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 20 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [36] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [37] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [39] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [40] "  A: 30.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [41] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [42] "  G: 70.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [43] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [44] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [45] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [46] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [47] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [48] "12\t5\t0.0\t1\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [49] "13\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [50] "26\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [51] "66\t12\t0.0\t2\t12"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [52] "67\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [53] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [54] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [55] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [56] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [57] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [58] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [59] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [60] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [61] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [62] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [63] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [64] "7\t1\t1.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [65] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [66] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [67] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [68] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [69] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 152 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [70] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [71] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [72] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [73] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [74] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [75] "  A: 63.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [76] "  C: 23.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [77] "  G: 3.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [78] "  T: 9.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [79] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [80] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [81] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [82] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [83] "5\t15\t16.3\t0\t15"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [84] "6\t1\t4.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [85] "7\t2\t1.0\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [86] "8\t8\t0.3\t0\t8"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [87] "9\t1\t0.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [88] "10\t3\t0.0\t1\t1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [89] "12\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [90] "14\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [91] "15\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [92] "16\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [93] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [94] "18\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [95] "20\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [96] "21\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [97] "22\t4\t0.0\t2\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [98] "23\t4\t0.0\t2\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [99] "24\t2\t0.0\t2\t0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [100] "27\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [101] "31\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [102] "32\t1\t0.0\t3\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [103] "34\t3\t0.0\t3\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [104] "35\t6\t0.0\t3\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [105] "36\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [106] "39\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [107] "40\t5\t0.0\t4\t3 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [108] "41\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [109] "42\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [110] "46\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [111] "47\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [112] "48\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [113] "52\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [114] "54\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [115] "55\t2\t0.0\t4\t1 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [116] "58\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [117] "59\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [118] "60\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [119] "61\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [120] "63\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [121] "65\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [122] "66\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [123] "67\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [124] "70\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [125] "72\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [126] "73\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [127] "74\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [128] "75\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [129] "79\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [130] "80\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [131] "82\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [132] "83\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [133] "84\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [134] "85\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [135] "86\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [136] "87\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [137] "91\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [138] "93\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [139] "94\t13\t0.0\t4\t10 2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [140] "98\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [141] "99\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [142] "102\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [143] "105\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [144] "107\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [145] "113\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [146] "117\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [147] "119\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [148] "121\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [149] "127\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [150] "128\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [151] "132\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [152] "135\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [153] "146\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [154] "154\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [155] "177\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## 
## [[6]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/104RNA1_S6_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/104RNA1_S6_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/104RNA1_S6_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/104RNA1_S6_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##   [4] "Finished in 0.55 s (117 us/read; 0.51 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##   [8] "Total read pairs processed:              4,721"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [9] "  Read 1 with adapter:                       8 (0.2%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [10] "  Read 2 with adapter:                     349 (7.4%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [11] "Pairs written (passing filters):         4,719 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [13] "Total basepairs processed:     1,982,820 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [14] "  Read 1:       925,316 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [15] "  Read 2:     1,057,504 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [16] "Total written (filtered):      1,957,358 bp (98.7%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [17] "  Read 1:       924,331 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [18] "  Read 2:     1,033,027 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [24] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [25] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [26] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 8 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [27] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [28] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [29] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [30] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [31] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [32] "  A: 12.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [33] "  C: 12.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [34] "  G: 75.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [35] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [36] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [38] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [39] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [40] "6\t1\t1.2\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [41] "10\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [42] "82\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [43] "88\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [44] "90\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [45] "92\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [46] "143\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [47] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [48] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [49] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [50] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [51] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 6 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [52] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [53] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [54] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [55] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [56] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [57] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [58] "5\t1\t4.6\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [59] "12\t5\t0.0\t1\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [60] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [61] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [62] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [63] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [64] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 344 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [65] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [66] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [67] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [68] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [69] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [70] "  A: 83.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [71] "  C: 13.4%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [72] "  G: 0.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [73] "  T: 2.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [74] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [75] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [76] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [77] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [78] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [79] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [80] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [81] "5\t1\t4.6\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [82] "6\t2\t1.2\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [83] "7\t2\t0.3\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [84] "8\t4\t0.1\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [85] "10\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [86] "11\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [87] "12\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [88] "13\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [89] "14\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [90] "15\t4\t0.0\t1\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [91] "16\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [92] "17\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [93] "18\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [94] "19\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [95] "20\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [96] "21\t5\t0.0\t2\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [97] "22\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [98] "23\t3\t0.0\t2\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [99] "24\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [100] "25\t4\t0.0\t2\t3 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [101] "26\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [102] "27\t4\t0.0\t2\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [103] "29\t2\t0.0\t2\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [104] "30\t2\t0.0\t3\t1 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [105] "32\t4\t0.0\t3\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [106] "33\t4\t0.0\t3\t1 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [107] "34\t4\t0.0\t3\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [108] "35\t3\t0.0\t3\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [109] "37\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [110] "38\t3\t0.0\t3\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [111] "39\t1\t0.0\t3\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [112] "40\t3\t0.0\t4\t1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [113] "41\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [114] "42\t7\t0.0\t4\t6 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [115] "43\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [116] "44\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [117] "45\t5\t0.0\t4\t3 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [118] "46\t6\t0.0\t4\t3 2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [119] "47\t5\t0.0\t4\t3 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [120] "49\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [121] "50\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [122] "51\t8\t0.0\t4\t7 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [123] "52\t11\t0.0\t4\t9 1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [124] "53\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [125] "54\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [126] "55\t3\t0.0\t4\t2 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [127] "56\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [128] "57\t7\t0.0\t4\t3 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [129] "58\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [130] "59\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [131] "60\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [132] "61\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [133] "63\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [134] "64\t7\t0.0\t4\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [135] "65\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [136] "66\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [137] "67\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [138] "69\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [139] "70\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [140] "72\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [141] "73\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [142] "74\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [143] "75\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [144] "76\t8\t0.0\t4\t8"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [145] "78\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [146] "79\t7\t0.0\t4\t6 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [147] "81\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [148] "82\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [149] "83\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [150] "84\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [151] "85\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [152] "86\t13\t0.0\t4\t11 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [153] "87\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [154] "88\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [155] "89\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [156] "91\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [157] "92\t3\t0.0\t4\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [158] "93\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [159] "94\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [160] "95\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [161] "96\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [162] "97\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [163] "99\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [164] "100\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [165] "101\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [166] "102\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [167] "103\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [168] "104\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [169] "105\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [170] "106\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [171] "107\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [172] "110\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [173] "111\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [174] "112\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [175] "113\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [176] "115\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [177] "116\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [178] "117\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [179] "118\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [180] "119\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [181] "120\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [182] "121\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [183] "122\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [184] "123\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [185] "125\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [186] "126\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [187] "127\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [188] "128\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [189] "129\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [190] "130\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [191] "131\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [192] "133\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [193] "134\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [194] "135\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [195] "137\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [196] "139\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [197] "140\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [198] "141\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [199] "142\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [200] "144\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [201] "146\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [202] "147\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [203] "148\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [204] "150\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [205] "156\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [206] "162\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [207] "166\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [208] "171\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [209] "173\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [210] "175\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [211] "180\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [212] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [213] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [214] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [215] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [216] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## 
## [[7]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/104RNA2_S7_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/104RNA2_S7_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/104RNA2_S7_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/104RNA2_S7_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##   [4] "Finished in 1.50 s (121 us/read; 0.49 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##   [8] "Total read pairs processed:             12,360"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [9] "  Read 1 with adapter:                      43 (0.3%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [10] "  Read 2 with adapter:                     547 (4.4%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [11] "Pairs written (passing filters):        12,359 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [13] "Total basepairs processed:     5,191,200 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [14] "  Read 1:     2,422,560 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [15] "  Read 2:     2,768,640 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [16] "Total written (filtered):      5,150,661 bp (99.2%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [17] "  Read 1:     2,419,806 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [18] "  Read 2:     2,730,855 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 5 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [29] "15\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [30] "18\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [31] "20\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [32] "31\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [33] "33\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [34] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [36] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [38] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 38 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [39] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [40] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [41] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [42] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [43] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [44] "  A: 18.4%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [45] "  C: 7.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [46] "  G: 73.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [47] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [48] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [49] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [50] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [51] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [52] "7\t1\t0.8\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [53] "10\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [54] "11\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [55] "13\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [56] "14\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [57] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [58] "36\t5\t0.0\t2\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [59] "44\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [60] "45\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [61] "56\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [62] "59\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [63] "72\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [64] "82\t4\t0.0\t2\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [65] "90\t8\t0.0\t2\t8"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [66] "91\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [67] "99\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [68] "116\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [69] "117\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [70] "118\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [71] "157\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [72] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [73] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [74] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [75] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [76] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 2 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [77] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [78] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [79] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [80] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [81] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [82] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [83] "5\t2\t12.1\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [84] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [85] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [86] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [87] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [88] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 546 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [89] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [90] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [91] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [92] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [93] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [94] "  A: 81.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [95] "  C: 12.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [96] "  G: 2.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [97] "  T: 3.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [98] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [99] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [100] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [101] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [102] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [103] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [104] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [105] "5\t4\t12.1\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [106] "6\t4\t3.0\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [107] "7\t5\t0.8\t0\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [108] "8\t4\t0.2\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [109] "9\t4\t0.0\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [110] "10\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [111] "11\t2\t0.0\t1\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [112] "12\t4\t0.0\t1\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [113] "13\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [114] "14\t8\t0.0\t1\t7 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [115] "15\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [116] "16\t9\t0.0\t1\t8 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [117] "17\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [118] "18\t3\t0.0\t1\t1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [119] "19\t3\t0.0\t1\t1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [120] "20\t7\t0.0\t2\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [121] "21\t4\t0.0\t2\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [122] "22\t4\t0.0\t2\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [123] "23\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [124] "24\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [125] "25\t8\t0.0\t2\t7 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [126] "26\t2\t0.0\t2\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [127] "27\t5\t0.0\t2\t3 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [128] "28\t3\t0.0\t2\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [129] "29\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [130] "30\t4\t0.0\t3\t3 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [131] "31\t6\t0.0\t3\t4 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [132] "32\t4\t0.0\t3\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [133] "33\t8\t0.0\t3\t5 2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [134] "34\t7\t0.0\t3\t5 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [135] "35\t6\t0.0\t3\t4 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [136] "36\t5\t0.0\t3\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [137] "37\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [138] "38\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [139] "39\t5\t0.0\t3\t2 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [140] "40\t4\t0.0\t4\t3 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [141] "41\t2\t0.0\t4\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [142] "42\t5\t0.0\t4\t4 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [143] "43\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [144] "44\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [145] "45\t5\t0.0\t4\t2 1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [146] "46\t8\t0.0\t4\t8"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [147] "47\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [148] "48\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [149] "49\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [150] "50\t7\t0.0\t4\t6 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [151] "51\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [152] "52\t13\t0.0\t4\t10 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [153] "53\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [154] "54\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [155] "55\t4\t0.0\t4\t2 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [156] "56\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [157] "57\t5\t0.0\t4\t4 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [158] "58\t6\t0.0\t4\t4 0 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [159] "59\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [160] "60\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [161] "61\t6\t0.0\t4\t5 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [162] "62\t3\t0.0\t4\t1 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [163] "63\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [164] "64\t10\t0.0\t4\t9 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [165] "65\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [166] "66\t2\t0.0\t4\t0 1 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [167] "67\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [168] "68\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [169] "69\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [170] "70\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [171] "71\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [172] "72\t8\t0.0\t4\t8"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [173] "73\t6\t0.0\t4\t2 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [174] "74\t7\t0.0\t4\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [175] "75\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [176] "76\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [177] "77\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [178] "78\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [179] "79\t6\t0.0\t4\t5 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [180] "80\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [181] "81\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [182] "82\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [183] "83\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [184] "84\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [185] "85\t7\t0.0\t4\t6 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [186] "86\t10\t0.0\t4\t10"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [187] "87\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [188] "88\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [189] "89\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [190] "90\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [191] "91\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [192] "92\t7\t0.0\t4\t6 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [193] "93\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [194] "94\t8\t0.0\t4\t7 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [195] "95\t6\t0.0\t4\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [196] "96\t8\t0.0\t4\t5 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [197] "97\t8\t0.0\t4\t7 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [198] "99\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [199] "100\t4\t0.0\t4\t2 1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [200] "101\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [201] "102\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [202] "103\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [203] "104\t9\t0.0\t4\t9"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [204] "105\t24\t0.0\t4\t24"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [205] "106\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [206] "107\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [207] "108\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [208] "109\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [209] "110\t9\t0.0\t4\t7 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [210] "111\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [211] "112\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [212] "113\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [213] "115\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [214] "117\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [215] "118\t10\t0.0\t4\t8 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [216] "119\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [217] "120\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [218] "121\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [219] "122\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [220] "125\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [221] "126\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [222] "127\t4\t0.0\t4\t2 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [223] "128\t2\t0.0\t4\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [224] "129\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [225] "130\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [226] "132\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [227] "134\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [228] "139\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [229] "142\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [230] "144\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [231] "145\t3\t0.0\t4\t0 0 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [232] "146\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [233] "154\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [234] "156\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [235] "159\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [236] "160\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [237] "161\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [238] "163\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [239] "164\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [240] "166\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [241] "167\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [242] "183\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [243] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [244] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [245] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [246] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [247] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## 
## [[8]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/104RNA3_S8_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/104RNA3_S8_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/104RNA3_S8_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/104RNA3_S8_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##   [4] "Finished in 1.68 s (117 us/read; 0.51 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##   [8] "Total read pairs processed:             14,346"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [9] "  Read 1 with adapter:                      23 (0.2%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [10] "  Read 2 with adapter:                     456 (3.2%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [11] "Pairs written (passing filters):        14,346 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [13] "Total basepairs processed:     6,025,320 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [14] "  Read 1:     2,811,816 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [15] "  Read 2:     3,213,504 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [16] "Total written (filtered):      5,991,740 bp (99.4%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [17] "  Read 1:     2,810,762 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [18] "  Read 2:     3,180,978 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 4 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [29] "8\t1\t0.2\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [30] "17\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [31] "37\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [32] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [34] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [36] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 19 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [38] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [39] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [40] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [41] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [42] "  A: 36.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [43] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [44] "  G: 63.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [45] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [46] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [47] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [48] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [49] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [50] "5\t1\t14.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [51] "8\t1\t0.2\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [52] "9\t1\t0.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [53] "12\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [54] "17\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [55] "36\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [56] "56\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [57] "71\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [58] "73\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [59] "75\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [60] "81\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [61] "82\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [62] "90\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [63] "91\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [64] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [65] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [66] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [67] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [68] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [69] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [70] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [71] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [72] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [73] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [74] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [75] "92\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [76] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [77] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [78] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [79] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [80] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 456 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [81] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [82] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [83] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [84] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [85] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [86] "  A: 86.4%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [87] "  C: 10.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [88] "  G: 1.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [89] "  T: 1.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [90] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [91] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [92] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [93] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [94] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [95] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [96] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [97] "5\t6\t14.0\t0\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [98] "6\t6\t3.5\t0\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [99] "7\t1\t0.9\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [100] "8\t4\t0.2\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [101] "9\t1\t0.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [102] "10\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [103] "11\t2\t0.0\t1\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [104] "12\t2\t0.0\t1\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [105] "14\t6\t0.0\t1\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [106] "15\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [107] "16\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [108] "17\t2\t0.0\t1\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [109] "18\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [110] "19\t2\t0.0\t1\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [111] "20\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [112] "21\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [113] "22\t6\t0.0\t2\t4 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [114] "23\t5\t0.0\t2\t2 2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [115] "24\t4\t0.0\t2\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [116] "25\t4\t0.0\t2\t2 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [117] "26\t1\t0.0\t2\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [118] "27\t5\t0.0\t2\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [119] "29\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [120] "30\t1\t0.0\t3\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [121] "31\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [122] "32\t5\t0.0\t3\t3 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [123] "33\t3\t0.0\t3\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [124] "34\t3\t0.0\t3\t2 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [125] "35\t4\t0.0\t3\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [126] "36\t3\t0.0\t3\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [127] "37\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [128] "38\t2\t0.0\t3\t0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [129] "39\t3\t0.0\t3\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [130] "40\t4\t0.0\t4\t2 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [131] "41\t9\t0.0\t4\t8 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [132] "42\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [133] "43\t6\t0.0\t4\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [134] "44\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [135] "45\t5\t0.0\t4\t3 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [136] "46\t10\t0.0\t4\t5 1 2 0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [137] "47\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [138] "48\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [139] "49\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [140] "50\t4\t0.0\t4\t3 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [141] "51\t8\t0.0\t4\t6 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [142] "52\t12\t0.0\t4\t10 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [143] "53\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [144] "54\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [145] "55\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [146] "56\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [147] "57\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [148] "58\t6\t0.0\t4\t4 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [149] "59\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [150] "60\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [151] "61\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [152] "62\t7\t0.0\t4\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [153] "63\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [154] "64\t9\t0.0\t4\t6 2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [155] "65\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [156] "66\t6\t0.0\t4\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [157] "67\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [158] "68\t3\t0.0\t4\t1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [159] "69\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [160] "70\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [161] "71\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [162] "72\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [163] "73\t4\t0.0\t4\t2 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [164] "74\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [165] "75\t7\t0.0\t4\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [166] "76\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [167] "77\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [168] "78\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [169] "79\t9\t0.0\t4\t8 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [170] "80\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [171] "81\t2\t0.0\t4\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [172] "82\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [173] "83\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [174] "84\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [175] "85\t9\t0.0\t4\t6 2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [176] "86\t9\t0.0\t4\t9"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [177] "87\t6\t0.0\t4\t5 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [178] "88\t5\t0.0\t4\t4 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [179] "89\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [180] "90\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [181] "91\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [182] "92\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [183] "93\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [184] "94\t6\t0.0\t4\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [185] "95\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [186] "96\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [187] "97\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [188] "99\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [189] "100\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [190] "101\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [191] "102\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [192] "103\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [193] "104\t3\t0.0\t4\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [194] "105\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [195] "107\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [196] "108\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [197] "109\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [198] "110\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [199] "111\t7\t0.0\t4\t6 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [200] "112\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [201] "113\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [202] "114\t4\t0.0\t4\t3 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [203] "115\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [204] "116\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [205] "117\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [206] "118\t9\t0.0\t4\t9"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [207] "119\t6\t0.0\t4\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [208] "120\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [209] "121\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [210] "123\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [211] "124\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [212] "125\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [213] "126\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [214] "127\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [215] "129\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [216] "130\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [217] "132\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [218] "133\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [219] "134\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [220] "139\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [221] "140\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [222] "141\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [223] "142\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [224] "146\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [225] "149\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [226] "150\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [227] "152\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [228] "154\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [229] "158\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [230] "159\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [231] "160\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [232] "161\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [233] "173\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [234] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [235] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [236] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [237] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [238] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## 
## [[9]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/1051_S9_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/1051_S9_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/1051_S9_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/1051_S9_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##   [4] "Finished in 1.66 s (113 us/read; 0.53 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [8] "Total read pairs processed:             14,658"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##   [9] "  Read 1 with adapter:                       4 (0.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [10] "  Read 2 with adapter:                     127 (0.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [11] "Pairs written (passing filters):        14,658 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [13] "Total basepairs processed:     6,156,360 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [14] "  Read 1:     2,872,968 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [15] "  Read 2:     3,283,392 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [16] "Total written (filtered):      6,147,694 bp (99.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [17] "  Read 1:     2,872,872 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [18] "  Read 2:     3,274,822 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 3 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [29] "5\t1\t14.3\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [30] "7\t1\t0.9\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [31] "14\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [32] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [34] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [36] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [38] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [39] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [40] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [41] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [42] "  A: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [43] "  C: 100.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [44] "  G: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [45] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [46] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [47] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [48] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [49] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [50] "70\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [51] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [52] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [53] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [54] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [55] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 2 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [56] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [57] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [58] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [59] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [60] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [61] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [62] "9\t1\t0.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [63] "24\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [64] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [65] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [66] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [67] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [68] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 125 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [69] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [70] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [71] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [72] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [73] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [74] "  A: 82.4%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [75] "  C: 11.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [76] "  G: 4.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [77] "  T: 1.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [78] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [79] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [80] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [81] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [82] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [83] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [84] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [85] "5\t10\t14.3\t0\t10"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [86] "6\t2\t3.6\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [87] "8\t2\t0.2\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [88] "10\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [89] "11\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [90] "16\t4\t0.0\t1\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [91] "21\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [92] "23\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [93] "24\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [94] "25\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [95] "27\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [96] "29\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [97] "30\t1\t0.0\t3\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [98] "31\t3\t0.0\t3\t1 0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [99] "32\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [100] "33\t4\t0.0\t3\t1 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [101] "34\t1\t0.0\t3\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [102] "35\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [103] "37\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [104] "39\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [105] "40\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [106] "44\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [107] "45\t3\t0.0\t4\t1 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [108] "46\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [109] "48\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [110] "51\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [111] "52\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [112] "57\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [113] "59\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [114] "61\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [115] "62\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [116] "63\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [117] "64\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [118] "65\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [119] "66\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [120] "68\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [121] "70\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [122] "71\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [123] "72\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [124] "77\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [125] "79\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [126] "80\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [127] "82\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [128] "84\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [129] "86\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [130] "88\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [131] "89\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [132] "92\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [133] "93\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [134] "94\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [135] "97\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [136] "98\t2\t0.0\t4\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [137] "102\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [138] "105\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [139] "106\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [140] "108\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [141] "111\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [142] "114\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [143] "119\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [144] "120\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [145] "123\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [146] "124\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [147] "126\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [148] "128\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [149] "129\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [150] "132\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [151] "135\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [152] "142\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [153] "144\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [154] "146\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [155] "148\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [156] "149\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [157] "150\t1\t0.0\t4\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [158] "156\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [159] "160\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [160] "164\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [161] "174\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [162] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [163] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [164] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [165] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [166] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## 
## [[10]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/1052_S10_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/1052_S10_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/1052_S10_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/1052_S10_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##   [4] "Finished in 1.51 s (110 us/read; 0.55 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [8] "Total read pairs processed:             13,743"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [9] "  Read 1 with adapter:                       1 (0.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [10] "  Read 2 with adapter:                     129 (0.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [11] "Pairs written (passing filters):        13,742 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [13] "Total basepairs processed:     5,772,060 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [14] "  Read 1:     2,693,628 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [15] "  Read 2:     3,078,432 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [16] "Total written (filtered):      5,764,252 bp (99.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [17] "  Read 1:     2,693,295 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [18] "  Read 2:     3,070,957 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [24] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [25] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [26] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [27] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [28] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [29] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [30] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [31] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [32] "  A: 100.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [33] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [34] "  G: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [35] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [36] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [38] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [39] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [40] "137\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [41] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [42] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [43] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [44] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [45] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [46] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [47] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [48] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [49] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 129 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [50] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [51] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [52] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [53] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [54] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [55] "  A: 79.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [56] "  C: 15.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [57] "  G: 3.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [58] "  T: 1.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [59] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [60] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [61] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [62] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [63] "5\t11\t13.4\t0\t11"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [64] "6\t1\t3.4\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [65] "7\t2\t0.8\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [66] "8\t2\t0.2\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [67] "9\t2\t0.1\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [68] "10\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [69] "12\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [70] "13\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [71] "14\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [72] "15\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [73] "16\t4\t0.0\t1\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [74] "17\t2\t0.0\t1\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [75] "19\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [76] "20\t2\t0.0\t2\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [77] "22\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [78] "24\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [79] "25\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [80] "26\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [81] "29\t2\t0.0\t2\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [82] "30\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [83] "31\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [84] "32\t3\t0.0\t3\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [85] "33\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [86] "34\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [87] "35\t3\t0.0\t3\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [88] "37\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [89] "38\t1\t0.0\t3\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [90] "42\t3\t0.0\t4\t1 0 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [91] "43\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [92] "44\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [93] "45\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [94] "47\t2\t0.0\t4\t1 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [95] "48\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [96] "51\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [97] "52\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [98] "53\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [99] "57\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [100] "58\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [101] "59\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [102] "64\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [103] "65\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [104] "69\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [105] "72\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [106] "73\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [107] "78\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [108] "79\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [109] "82\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [110] "85\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [111] "86\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [112] "87\t3\t0.0\t4\t1 1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [113] "88\t2\t0.0\t4\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [114] "91\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [115] "94\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [116] "98\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [117] "101\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [118] "104\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [119] "105\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [120] "106\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [121] "108\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [122] "109\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [123] "111\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [124] "118\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [125] "120\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [126] "121\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [127] "128\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [128] "130\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [129] "135\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [130] "139\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [131] "140\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [132] "142\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [133] "143\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [134] "144\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [135] "180\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## 
## [[11]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/1053_S11_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/1053_S11_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/1053_S11_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/1053_S11_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##   [4] "Finished in 1.23 s (112 us/read; 0.54 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [8] "Total read pairs processed:             10,965"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [9] "  Read 1 with adapter:                       6 (0.1%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [10] "  Read 2 with adapter:                      90 (0.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [11] "Pairs written (passing filters):        10,965 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [13] "Total basepairs processed:     4,605,300 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [14] "  Read 1:     2,149,140 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [15] "  Read 2:     2,456,160 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [16] "Total written (filtered):      4,599,637 bp (99.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [17] "  Read 1:     2,148,747 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [18] "  Read 2:     2,450,890 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 2 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [29] "8\t1\t0.2\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [30] "28\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [32] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [33] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [34] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [35] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 4 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [36] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [37] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [38] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [39] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [40] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [41] "  A: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [42] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [43] "  G: 100.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [44] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [45] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [46] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [47] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [48] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [49] "34\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [50] "41\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [51] "141\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [52] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [53] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [54] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [55] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [56] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [57] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [58] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [59] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [60] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [61] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [62] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [63] "73\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [64] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [65] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [66] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [67] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [68] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 89 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [69] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [70] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [71] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [72] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [73] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [74] "  A: 86.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [75] "  C: 6.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [76] "  G: 4.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [77] "  T: 2.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [78] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [79] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [80] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [81] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [82] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [83] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [84] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [85] "5\t7\t10.7\t0\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [86] "6\t1\t2.7\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [87] "8\t1\t0.2\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [88] "9\t1\t0.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [89] "11\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [90] "12\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [91] "13\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [92] "16\t3\t0.0\t1\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [93] "20\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [94] "22\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [95] "30\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [96] "31\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [97] "32\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [98] "33\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [99] "34\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [100] "35\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [101] "36\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [102] "39\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [103] "40\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [104] "41\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [105] "42\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [106] "43\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [107] "44\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [108] "46\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [109] "48\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [110] "51\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [111] "52\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [112] "53\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [113] "55\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [114] "56\t2\t0.0\t4\t1 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [115] "57\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [116] "58\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [117] "62\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [118] "63\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [119] "64\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [120] "68\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [121] "71\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [122] "72\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [123] "73\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [124] "74\t2\t0.0\t4\t0 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [125] "77\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [126] "78\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [127] "79\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [128] "81\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [129] "83\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [130] "84\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [131] "85\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [132] "86\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [133] "90\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [134] "91\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [135] "94\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [136] "99\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [137] "100\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [138] "102\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [139] "103\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [140] "106\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [141] "107\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [142] "113\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [143] "116\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [144] "118\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [145] "122\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [146] "129\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [147] "144\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [148] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [149] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [150] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [151] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [152] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## 
## [[12]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/105RNA1_S12_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/105RNA1_S12_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/105RNA1_S12_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/105RNA1_S12_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##   [4] "Finished in 0.68 s (105 us/read; 0.57 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [8] "Total read pairs processed:              6,497"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##   [9] "  Read 1 with adapter:                      21 (0.3%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [10] "  Read 2 with adapter:                     134 (2.1%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [11] "Pairs written (passing filters):         6,497 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [13] "Total basepairs processed:     2,728,740 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [14] "  Read 1:     1,273,412 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [15] "  Read 2:     1,455,328 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [16] "Total written (filtered):      2,719,072 bp (99.6%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [17] "  Read 1:     1,272,441 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [18] "  Read 2:     1,446,631 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [29] "10\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [30] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [32] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [34] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 20 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [36] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [37] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [39] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [40] "  A: 20.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [41] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [42] "  G: 80.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [43] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [44] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [45] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [46] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [47] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [48] "8\t1\t0.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [49] "10\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [50] "15\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [51] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [52] "36\t6\t0.0\t2\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [53] "59\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [54] "63\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [55] "90\t5\t0.0\t2\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [56] "98\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [57] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [58] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [59] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [60] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [61] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [62] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [63] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [64] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [65] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 134 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [66] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [67] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [68] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [69] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [70] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [71] "  A: 93.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [72] "  C: 3.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [73] "  G: 1.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [74] "  T: 1.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [75] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [76] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [77] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [78] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [79] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [80] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [81] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [82] "5\t6\t6.3\t0\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [83] "9\t1\t0.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [84] "10\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [85] "14\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [86] "16\t4\t0.0\t1\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [87] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [88] "19\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [89] "20\t1\t0.0\t2\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [90] "23\t3\t0.0\t2\t1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [91] "25\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [92] "27\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [93] "29\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [94] "32\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [95] "33\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [96] "34\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [97] "35\t4\t0.0\t3\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [98] "36\t3\t0.0\t3\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [99] "37\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [100] "39\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [101] "40\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [102] "41\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [103] "42\t1\t0.0\t4\t0 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [104] "43\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [105] "45\t2\t0.0\t4\t0 1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [106] "46\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [107] "47\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [108] "48\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [109] "49\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [110] "52\t4\t0.0\t4\t2 1 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [111] "54\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [112] "56\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [113] "57\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [114] "59\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [115] "63\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [116] "64\t6\t0.0\t4\t4 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [117] "65\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [118] "66\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [119] "69\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [120] "70\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [121] "71\t2\t0.0\t4\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [122] "72\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [123] "75\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [124] "76\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [125] "77\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [126] "78\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [127] "82\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [128] "84\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [129] "86\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [130] "87\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [131] "89\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [132] "91\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [133] "92\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [134] "93\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [135] "94\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [136] "95\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [137] "96\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [138] "98\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [139] "100\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [140] "102\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [141] "103\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [142] "104\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [143] "105\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [144] "110\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [145] "111\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [146] "112\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [147] "115\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [148] "116\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [149] "118\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [150] "120\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [151] "121\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [152] "126\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [153] "129\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [154] "141\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [155] "154\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [156] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [157] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [158] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [159] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [160] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## 
## [[13]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/105RNA2_S13_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/105RNA2_S13_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/105RNA2_S13_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/105RNA2_S13_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##   [4] "Finished in 0.80 s (103 us/read; 0.58 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [8] "Total read pairs processed:              7,754"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##   [9] "  Read 1 with adapter:                      17 (0.2%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [10] "  Read 2 with adapter:                     159 (2.1%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [11] "Pairs written (passing filters):         7,753 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [13] "Total basepairs processed:     3,256,680 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [14] "  Read 1:     1,519,784 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [15] "  Read 2:     1,736,896 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [16] "Total written (filtered):      3,245,708 bp (99.7%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [17] "  Read 1:     1,518,688 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [18] "  Read 2:     1,727,020 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [24] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [25] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [26] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 17 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [27] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [28] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [29] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [30] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [31] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [32] "  A: 47.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [33] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [34] "  G: 52.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [35] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [36] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [38] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [39] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [40] "9\t2\t0.0\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [41] "14\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [42] "16\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [43] "17\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [44] "19\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [45] "36\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [46] "70\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [47] "91\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [48] "118\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [49] "137\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [50] "140\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [51] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [52] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [53] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [54] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [55] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [56] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [57] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [58] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [59] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [60] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [61] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [62] "6\t1\t1.9\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [63] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [64] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [65] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [66] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [67] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 159 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [68] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [69] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [70] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [71] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [72] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [73] "  A: 96.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [74] "  C: 3.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [75] "  G: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [76] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [77] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [78] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [79] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [80] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [81] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [82] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [83] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [84] "5\t2\t7.6\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [85] "7\t1\t0.5\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [86] "8\t1\t0.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [87] "11\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [88] "13\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [89] "14\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [90] "15\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [91] "16\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [92] "17\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [93] "19\t4\t0.0\t1\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [94] "20\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [95] "21\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [96] "22\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [97] "23\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [98] "24\t2\t0.0\t2\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [99] "25\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [100] "26\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [101] "27\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [102] "28\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [103] "30\t3\t0.0\t3\t1 1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [104] "31\t1\t0.0\t3\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [105] "33\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [106] "34\t5\t0.0\t3\t4 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [107] "35\t4\t0.0\t3\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [108] "36\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [109] "37\t3\t0.0\t3\t1 1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [110] "38\t2\t0.0\t3\t0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [111] "39\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [112] "40\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [113] "41\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [114] "42\t2\t0.0\t4\t0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [115] "43\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [116] "44\t3\t0.0\t4\t1 1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [117] "45\t6\t0.0\t4\t3 2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [118] "46\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [119] "47\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [120] "48\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [121] "50\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [122] "51\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [123] "52\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [124] "53\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [125] "54\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [126] "56\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [127] "58\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [128] "59\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [129] "61\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [130] "62\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [131] "63\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [132] "64\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [133] "65\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [134] "66\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [135] "67\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [136] "68\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [137] "72\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [138] "77\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [139] "79\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [140] "81\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [141] "83\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [142] "84\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [143] "86\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [144] "87\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [145] "88\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [146] "90\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [147] "91\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [148] "94\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [149] "96\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [150] "98\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [151] "99\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [152] "104\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [153] "105\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [154] "107\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [155] "111\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [156] "112\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [157] "113\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [158] "114\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [159] "115\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [160] "118\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [161] "119\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [162] "120\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [163] "121\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [164] "128\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [165] "129\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [166] "131\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [167] "143\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [168] "145\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [169] "146\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [170] "147\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [171] "159\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [172] "178\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [173] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [174] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [175] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [176] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [177] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## 
## [[14]]
##  [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/105RNA3_S14_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/105RNA3_S14_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/105RNA3_S14_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/105RNA3_S14_L001_R2_001.fastq.gz"
##  [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [4] "Finished in 0.02 s (272 us/read; 0.22 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [8] "Total read pairs processed:                 65"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [9] "  Read 1 with adapter:                       2 (3.1%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [10] "  Read 2 with adapter:                       6 (9.2%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [11] "Pairs written (passing filters):            65 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [13] "Total basepairs processed:        27,300 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [14] "  Read 1:        12,740 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [15] "  Read 2:        14,560 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [16] "Total written (filtered):         26,526 bp (97.2%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [17] "  Read 1:        12,540 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [18] "  Read 2:        13,986 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [24] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [25] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [26] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 2 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [27] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [28] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [29] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [30] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [31] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [32] "  A: 50.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [33] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [34] "  G: 50.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [35] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [36] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [38] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [39] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [40] "61\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [41] "139\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [42] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [43] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [44] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [45] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [46] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [47] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [48] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [49] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [50] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 6 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [51] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [52] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [53] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [54] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [55] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [56] "  A: 83.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [57] "  C: 16.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [58] "  G: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [59] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [60] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [61] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [62] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [63] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [64] "85\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [65] "86\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [66] "89\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [67] "97\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [68] "131\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## 
## [[15]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/14B1_S15_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/14B1_S15_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/14B1_S15_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/14B1_S15_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##   [4] "Finished in 4.41 s (106 us/read; 0.56 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [8] "Total read pairs processed:             41,426"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [9] "  Read 1 with adapter:                      15 (0.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [10] "  Read 2 with adapter:                     374 (0.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [11] "Pairs written (passing filters):        41,425 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [13] "Total basepairs processed:    17,398,920 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [14] "  Read 1:     8,119,496 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [15] "  Read 2:     9,279,424 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [16] "Total written (filtered):     17,375,613 bp (99.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [17] "  Read 1:     8,118,895 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [18] "  Read 2:     9,256,718 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 11 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [29] "5\t2\t40.5\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [30] "6\t1\t10.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [31] "7\t1\t2.5\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [32] "8\t1\t0.6\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [33] "15\t2\t0.0\t1\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [34] "16\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [35] "23\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [36] "33\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [37] "34\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [39] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [40] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [41] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [42] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 4 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [43] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [44] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [45] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [46] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [47] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [48] "  A: 50.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [49] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [50] "  G: 25.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [51] "  T: 25.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [52] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [53] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [54] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [55] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [56] "5\t1\t40.5\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [57] "44\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [58] "58\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [59] "131\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [60] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [61] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [62] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [63] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [64] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [65] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [66] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [67] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [68] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [69] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [70] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [71] "6\t1\t10.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [72] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [73] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [74] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [75] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [76] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 374 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [77] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [78] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [79] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [80] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [81] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [82] "  A: 59.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [83] "  C: 23.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [84] "  G: 15.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [85] "  T: 0.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [86] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [87] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [88] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [89] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [90] "5\t11\t40.5\t0\t11"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [91] "6\t4\t10.1\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [92] "7\t3\t2.5\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [93] "8\t6\t0.6\t0\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [94] "9\t4\t0.2\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [95] "10\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [96] "11\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [97] "12\t8\t0.0\t1\t8"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [98] "13\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [99] "14\t5\t0.0\t1\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [100] "15\t5\t0.0\t1\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [101] "16\t10\t0.0\t1\t10"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [102] "17\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [103] "19\t3\t0.0\t1\t0 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [104] "20\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [105] "21\t2\t0.0\t2\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [106] "22\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [107] "23\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [108] "24\t7\t0.0\t2\t6 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [109] "25\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [110] "26\t2\t0.0\t2\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [111] "29\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [112] "30\t4\t0.0\t3\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [113] "31\t3\t0.0\t3\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [114] "32\t4\t0.0\t3\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [115] "33\t2\t0.0\t3\t0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [116] "34\t4\t0.0\t3\t3 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [117] "35\t6\t0.0\t3\t4 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [118] "36\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [119] "37\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [120] "38\t9\t0.0\t3\t9"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [121] "39\t6\t0.0\t3\t4 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [122] "40\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [123] "41\t4\t0.0\t4\t2 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [124] "42\t7\t0.0\t4\t3 3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [125] "43\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [126] "44\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [127] "45\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [128] "46\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [129] "47\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [130] "48\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [131] "50\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [132] "51\t6\t0.0\t4\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [133] "52\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [134] "53\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [135] "54\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [136] "55\t4\t0.0\t4\t2 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [137] "56\t3\t0.0\t4\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [138] "57\t5\t0.0\t4\t4 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [139] "58\t5\t0.0\t4\t3 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [140] "59\t3\t0.0\t4\t2 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [141] "60\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [142] "62\t6\t0.0\t4\t4 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [143] "63\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [144] "64\t9\t0.0\t4\t9"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [145] "65\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [146] "66\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [147] "69\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [148] "70\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [149] "72\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [150] "73\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [151] "74\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [152] "75\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [153] "76\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [154] "77\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [155] "78\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [156] "79\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [157] "82\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [158] "83\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [159] "84\t7\t0.0\t4\t6 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [160] "85\t7\t0.0\t4\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [161] "86\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [162] "87\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [163] "89\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [164] "90\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [165] "91\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [166] "92\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [167] "93\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [168] "94\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [169] "95\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [170] "96\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [171] "98\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [172] "99\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [173] "100\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [174] "101\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [175] "102\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [176] "103\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [177] "104\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [178] "105\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [179] "106\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [180] "108\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [181] "109\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [182] "110\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [183] "111\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [184] "112\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [185] "114\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [186] "115\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [187] "117\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [188] "118\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [189] "119\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [190] "120\t3\t0.0\t4\t1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [191] "123\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [192] "124\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [193] "125\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [194] "129\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [195] "131\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [196] "132\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [197] "136\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [198] "137\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [199] "138\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [200] "144\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [201] "145\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [202] "147\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [203] "152\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [204] "159\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [205] "163\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [206] "171\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [207] "178\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## 
## [[16]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/14B2_S16_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/14B2_S16_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/14B2_S16_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/14B2_S16_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##   [4] "Finished in 3.11 s (106 us/read; 0.57 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [8] "Total read pairs processed:             29,473"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [9] "  Read 1 with adapter:                       8 (0.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [10] "  Read 2 with adapter:                     273 (0.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [11] "Pairs written (passing filters):        29,472 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [13] "Total basepairs processed:    12,378,660 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [14] "  Read 1:     5,776,708 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [15] "  Read 2:     6,601,952 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [16] "Total written (filtered):     12,361,149 bp (99.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [17] "  Read 1:     5,776,404 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [18] "  Read 2:     6,584,745 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 8 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [29] "5\t3\t28.8\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [30] "7\t1\t1.8\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [31] "10\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [32] "19\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [33] "26\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [34] "31\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [36] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [37] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [39] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [40] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [41] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [42] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [43] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [44] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [45] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [46] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [47] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [48] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [49] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [50] "5\t1\t28.8\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [51] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [52] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [53] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [54] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [55] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 273 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [56] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [57] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [58] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [59] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [60] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [61] "  A: 55.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [62] "  C: 20.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [63] "  G: 23.4%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [64] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [65] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [66] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [67] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [68] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [69] "5\t5\t28.8\t0\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [70] "6\t3\t7.2\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [71] "7\t4\t1.8\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [72] "8\t4\t0.4\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [73] "9\t4\t0.1\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [74] "10\t4\t0.0\t1\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [75] "12\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [76] "13\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [77] "14\t6\t0.0\t1\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [78] "15\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [79] "16\t9\t0.0\t1\t8 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [80] "17\t4\t0.0\t1\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [81] "18\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [82] "19\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [83] "20\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [84] "21\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [85] "22\t5\t0.0\t2\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [86] "23\t5\t0.0\t2\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [87] "24\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [88] "25\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [89] "27\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [90] "29\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [91] "30\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [92] "32\t3\t0.0\t3\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [93] "34\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [94] "36\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [95] "38\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [96] "39\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [97] "40\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [98] "41\t4\t0.0\t4\t2 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [99] "42\t4\t0.0\t4\t2 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [100] "43\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [101] "44\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [102] "46\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [103] "47\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [104] "48\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [105] "50\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [106] "51\t6\t0.0\t4\t3 2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [107] "52\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [108] "53\t5\t0.0\t4\t4 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [109] "54\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [110] "55\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [111] "56\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [112] "57\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [113] "58\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [114] "59\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [115] "60\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [116] "61\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [117] "62\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [118] "64\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [119] "65\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [120] "66\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [121] "67\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [122] "70\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [123] "72\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [124] "73\t5\t0.0\t4\t4 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [125] "74\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [126] "75\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [127] "76\t6\t0.0\t4\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [128] "77\t6\t0.0\t4\t5 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [129] "78\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [130] "79\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [131] "80\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [132] "81\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [133] "83\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [134] "84\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [135] "85\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [136] "86\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [137] "87\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [138] "89\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [139] "90\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [140] "91\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [141] "92\t2\t0.0\t4\t1 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [142] "93\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [143] "94\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [144] "95\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [145] "96\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [146] "97\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [147] "98\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [148] "101\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [149] "102\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [150] "103\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [151] "105\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [152] "109\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [153] "111\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [154] "112\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [155] "113\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [156] "115\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [157] "116\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [158] "118\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [159] "119\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [160] "120\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [161] "121\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [162] "125\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [163] "127\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [164] "130\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [165] "131\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [166] "140\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [167] "142\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [168] "143\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [169] "148\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [170] "149\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [171] "152\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [172] "153\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [173] "157\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [174] "158\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [175] "160\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [176] "164\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [177] "178\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## 
## [[17]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/14B3_S17_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/14B3_S17_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/14B3_S17_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/14B3_S17_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##   [4] "Finished in 3.01 s (104 us/read; 0.58 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [8] "Total read pairs processed:             29,025"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [9] "  Read 1 with adapter:                      12 (0.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [10] "  Read 2 with adapter:                     278 (1.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [11] "Pairs written (passing filters):        29,025 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [13] "Total basepairs processed:    12,190,500 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [14] "  Read 1:     5,688,900 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [15] "  Read 2:     6,501,600 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [16] "Total written (filtered):     12,171,800 bp (99.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [17] "  Read 1:     5,688,370 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [18] "  Read 2:     6,483,430 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 9 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [29] "6\t1\t7.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [30] "7\t1\t1.8\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [31] "12\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [32] "16\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [33] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [34] "18\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [35] "20\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [36] "22\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [37] "23\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [39] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [40] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [41] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [42] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 3 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [43] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [44] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [45] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [46] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [47] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [48] "  A: 33.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [49] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [50] "  G: 33.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [51] "  T: 33.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [52] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [53] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [54] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [55] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [56] "112\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [57] "131\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [58] "146\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [59] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [60] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [61] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [62] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [63] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [64] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [65] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [66] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [67] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 278 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [68] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [69] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [70] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [71] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [72] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [73] "  A: 58.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [74] "  C: 21.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [75] "  G: 19.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [76] "  T: 0.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [77] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [78] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [79] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [80] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [81] "5\t9\t28.3\t0\t9"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [82] "6\t3\t7.1\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [83] "7\t2\t1.8\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [84] "8\t4\t0.4\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [85] "9\t3\t0.1\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [86] "10\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [87] "11\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [88] "12\t5\t0.0\t1\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [89] "14\t14\t0.0\t1\t14"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [90] "15\t4\t0.0\t1\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [91] "16\t7\t0.0\t1\t5 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [92] "17\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [93] "18\t3\t0.0\t1\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [94] "19\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [95] "20\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [96] "21\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [97] "22\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [98] "23\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [99] "24\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [100] "25\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [101] "26\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [102] "27\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [103] "28\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [104] "31\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [105] "32\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [106] "33\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [107] "34\t3\t0.0\t3\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [108] "36\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [109] "38\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [110] "39\t3\t0.0\t3\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [111] "41\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [112] "43\t2\t0.0\t4\t0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [113] "44\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [114] "45\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [115] "50\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [116] "51\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [117] "52\t5\t0.0\t4\t3 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [118] "53\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [119] "55\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [120] "56\t7\t0.0\t4\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [121] "57\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [122] "58\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [123] "59\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [124] "60\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [125] "61\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [126] "62\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [127] "63\t6\t0.0\t4\t4 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [128] "64\t4\t0.0\t4\t1 2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [129] "65\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [130] "66\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [131] "67\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [132] "69\t3\t0.0\t4\t1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [133] "70\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [134] "71\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [135] "72\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [136] "73\t3\t0.0\t4\t1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [137] "74\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [138] "75\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [139] "77\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [140] "79\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [141] "80\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [142] "81\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [143] "82\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [144] "83\t3\t0.0\t4\t2 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [145] "84\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [146] "85\t8\t0.0\t4\t7 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [147] "86\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [148] "87\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [149] "88\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [150] "90\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [151] "91\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [152] "93\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [153] "94\t4\t0.0\t4\t2 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [154] "98\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [155] "99\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [156] "100\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [157] "101\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [158] "102\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [159] "103\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [160] "105\t8\t0.0\t4\t8"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [161] "107\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [162] "109\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [163] "110\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [164] "112\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [165] "113\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [166] "115\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [167] "117\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [168] "118\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [169] "119\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [170] "120\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [171] "121\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [172] "124\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [173] "125\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [174] "126\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [175] "127\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [176] "128\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [177] "130\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [178] "131\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [179] "133\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [180] "136\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [181] "138\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [182] "140\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [183] "141\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [184] "142\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [185] "144\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [186] "145\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [187] "147\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [188] "151\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [189] "156\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [190] "159\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [191] "162\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [192] "170\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [193] "171\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## 
## [[18]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/14B4_S18_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/14B4_S18_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/14B4_S18_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/14B4_S18_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##   [4] "Finished in 1.67 s (107 us/read; 0.56 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [8] "Total read pairs processed:             15,610"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [9] "  Read 1 with adapter:                      11 (0.1%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [10] "  Read 2 with adapter:                     168 (1.1%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [11] "Pairs written (passing filters):        15,609 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [13] "Total basepairs processed:     6,556,200 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [14] "  Read 1:     3,059,560 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [15] "  Read 2:     3,496,640 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [16] "Total written (filtered):      6,544,266 bp (99.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [17] "  Read 1:     3,058,822 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [18] "  Read 2:     3,485,444 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 5 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [29] "5\t1\t15.2\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [30] "6\t2\t3.8\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [31] "11\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [32] "16\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [34] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [35] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [36] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [37] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 6 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [39] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [40] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [41] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [42] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [43] "  A: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [44] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [45] "  G: 100.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [46] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [47] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [48] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [49] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [50] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [51] "19\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [52] "90\t4\t0.0\t2\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [53] "119\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [54] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [55] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [56] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [57] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [58] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [59] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [60] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [61] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [62] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [63] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [64] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [65] "55\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [66] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [67] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [68] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [69] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [70] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 167 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [71] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [72] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [73] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [74] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [75] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [76] "  A: 63.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [77] "  C: 13.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [78] "  G: 20.4%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [79] "  T: 2.4%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [80] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [81] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [82] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [83] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [84] "5\t3\t15.2\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [85] "6\t1\t3.8\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [86] "7\t2\t1.0\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [87] "8\t6\t0.2\t0\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [88] "9\t3\t0.1\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [89] "12\t2\t0.0\t1\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [90] "14\t4\t0.0\t1\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [91] "15\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [92] "16\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [93] "18\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [94] "19\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [95] "20\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [96] "22\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [97] "23\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [98] "24\t2\t0.0\t2\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [99] "25\t2\t0.0\t2\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [100] "26\t2\t0.0\t2\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [101] "27\t2\t0.0\t2\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [102] "28\t1\t0.0\t2\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [103] "29\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [104] "30\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [105] "31\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [106] "32\t4\t0.0\t3\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [107] "33\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [108] "34\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [109] "35\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [110] "36\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [111] "39\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [112] "42\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [113] "43\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [114] "44\t4\t0.0\t4\t2 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [115] "45\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [116] "46\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [117] "47\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [118] "49\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [119] "51\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [120] "52\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [121] "54\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [122] "57\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [123] "58\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [124] "59\t2\t0.0\t4\t1 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [125] "60\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [126] "63\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [127] "64\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [128] "65\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [129] "66\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [130] "67\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [131] "69\t1\t0.0\t4\t0 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [132] "72\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [133] "73\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [134] "74\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [135] "75\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [136] "77\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [137] "79\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [138] "81\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [139] "82\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [140] "84\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [141] "85\t4\t0.0\t4\t3 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [142] "86\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [143] "92\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [144] "93\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [145] "94\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [146] "95\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [147] "96\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [148] "97\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [149] "99\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [150] "105\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [151] "106\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [152] "108\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [153] "109\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [154] "111\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [155] "112\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [156] "114\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [157] "115\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [158] "116\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [159] "117\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [160] "118\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [161] "119\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [162] "120\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [163] "123\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [164] "124\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [165] "125\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [166] "126\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [167] "127\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [168] "128\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [169] "129\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [170] "135\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [171] "138\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [172] "140\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [173] "146\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [174] "147\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [175] "151\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [176] "160\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [177] "173\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [178] "184\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## 
## [[19]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/14BRNA1_S19_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/14BRNA1_S19_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/14BRNA1_S19_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/14BRNA1_S19_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##   [4] "Finished in 0.88 s (112 us/read; 0.54 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [8] "Total read pairs processed:              7,870"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##   [9] "  Read 1 with adapter:                     206 (2.6%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [10] "  Read 2 with adapter:                     711 (9.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [11] "Pairs written (passing filters):         7,867 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [13] "Total basepairs processed:     3,305,400 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [14] "  Read 1:     1,542,520 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [15] "  Read 2:     1,762,880 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [16] "Total written (filtered):      3,233,958 bp (97.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [17] "  Read 1:     1,528,627 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [18] "  Read 2:     1,705,331 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 4 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [29] "12\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [30] "33\t2\t0.0\t3\t0 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [31] "35\t1\t0.0\t3\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [32] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [34] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [36] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 202 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [38] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [39] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [40] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [41] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [42] "  A: 74.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [43] "  C: 2.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [44] "  G: 17.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [45] "  T: 5.4%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [46] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [47] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [48] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [49] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [50] "9\t1\t0.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [51] "11\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [52] "15\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [53] "20\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [54] "28\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [55] "36\t4\t0.0\t2\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [56] "54\t5\t0.0\t2\t3 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [57] "55\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [58] "56\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [59] "59\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [60] "60\t4\t0.0\t2\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [61] "61\t124\t0.0\t2\t123 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [62] "62\t6\t0.0\t2\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [63] "63\t2\t0.0\t2\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [64] "64\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [65] "67\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [66] "70\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [67] "74\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [68] "78\t2\t0.0\t2\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [69] "81\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [70] "82\t7\t0.0\t2\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [71] "84\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [72] "90\t20\t0.0\t2\t20"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [73] "92\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [74] "96\t4\t0.0\t2\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [75] "111\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [76] "119\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [77] "123\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [78] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [79] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [80] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [81] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [82] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [83] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [84] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [85] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [86] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [87] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [88] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [89] "5\t1\t7.7\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [90] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [91] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [92] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [93] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [94] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 711 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [95] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [96] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [97] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [98] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [99] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [100] "  A: 69.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [101] "  C: 12.4%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [102] "  G: 18.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [103] "  T: 0.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [104] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [105] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [106] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [107] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [108] "5\t2\t7.7\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [109] "6\t5\t1.9\t0\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [110] "7\t1\t0.5\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [111] "8\t7\t0.1\t0\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [112] "11\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [113] "12\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [114] "13\t2\t0.0\t1\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [115] "14\t9\t0.0\t1\t5 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [116] "15\t7\t0.0\t1\t6 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [117] "16\t10\t0.0\t1\t9 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [118] "17\t4\t0.0\t1\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [119] "18\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [120] "19\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [121] "20\t4\t0.0\t2\t1 2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [122] "21\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [123] "22\t3\t0.0\t2\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [124] "23\t2\t0.0\t2\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [125] "24\t4\t0.0\t2\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [126] "25\t3\t0.0\t2\t1 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [127] "26\t4\t0.0\t2\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [128] "27\t3\t0.0\t2\t1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [129] "28\t2\t0.0\t2\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [130] "30\t4\t0.0\t3\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [131] "31\t6\t0.0\t3\t2 2 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [132] "32\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [133] "33\t3\t0.0\t3\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [134] "34\t5\t0.0\t3\t3 1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [135] "35\t7\t0.0\t3\t5 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [136] "37\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [137] "38\t2\t0.0\t3\t0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [138] "39\t5\t0.0\t3\t1 2 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [139] "40\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [140] "41\t5\t0.0\t4\t3 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [141] "42\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [142] "43\t5\t0.0\t4\t3 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [143] "44\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [144] "45\t6\t0.0\t4\t3 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [145] "46\t6\t0.0\t4\t3 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [146] "47\t3\t0.0\t4\t0 1 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [147] "48\t7\t0.0\t4\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [148] "49\t3\t0.0\t4\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [149] "50\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [150] "51\t4\t0.0\t4\t2 0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [151] "52\t8\t0.0\t4\t3 2 2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [152] "53\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [153] "54\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [154] "55\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [155] "56\t7\t0.0\t4\t3 2 0 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [156] "57\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [157] "58\t7\t0.0\t4\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [158] "59\t6\t0.0\t4\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [159] "60\t2\t0.0\t4\t1 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [160] "61\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [161] "62\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [162] "63\t6\t0.0\t4\t3 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [163] "64\t14\t0.0\t4\t10 2 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [164] "65\t5\t0.0\t4\t3 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [165] "66\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [166] "67\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [167] "68\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [168] "70\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [169] "71\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [170] "72\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [171] "73\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [172] "74\t5\t0.0\t4\t3 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [173] "75\t10\t0.0\t4\t5 4 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [174] "76\t6\t0.0\t4\t2 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [175] "77\t6\t0.0\t4\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [176] "78\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [177] "79\t8\t0.0\t4\t6 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [178] "80\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [179] "81\t5\t0.0\t4\t3 1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [180] "82\t8\t0.0\t4\t3 4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [181] "83\t10\t0.0\t4\t6 3 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [182] "84\t13\t0.0\t4\t10 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [183] "85\t10\t0.0\t4\t8 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [184] "86\t14\t0.0\t4\t9 5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [185] "87\t4\t0.0\t4\t1 2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [186] "88\t8\t0.0\t4\t2 5 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [187] "89\t133\t0.0\t4\t8 100 18 4 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [188] "90\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [189] "91\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [190] "92\t6\t0.0\t4\t4 1 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [191] "93\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [192] "94\t12\t0.0\t4\t8 2 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [193] "95\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [194] "96\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [195] "97\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [196] "98\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [197] "99\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [198] "101\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [199] "102\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [200] "103\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [201] "104\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [202] "105\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [203] "106\t6\t0.0\t4\t4 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [204] "107\t3\t0.0\t4\t1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [205] "108\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [206] "109\t7\t0.0\t4\t3 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [207] "110\t13\t0.0\t4\t4 9"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [208] "111\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [209] "112\t5\t0.0\t4\t2 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [210] "113\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [211] "115\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [212] "116\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [213] "117\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [214] "118\t24\t0.0\t4\t8 13 2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [215] "119\t4\t0.0\t4\t3 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [216] "120\t5\t0.0\t4\t3 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [217] "121\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [218] "122\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [219] "123\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [220] "124\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [221] "125\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [222] "126\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [223] "127\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [224] "128\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [225] "130\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [226] "131\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [227] "132\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [228] "133\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [229] "134\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [230] "136\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [231] "137\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [232] "139\t6\t0.0\t4\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [233] "140\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [234] "141\t5\t0.0\t4\t4 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [235] "142\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [236] "145\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [237] "146\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [238] "147\t4\t0.0\t4\t2 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [239] "148\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [240] "149\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [241] "150\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [242] "151\t2\t0.0\t4\t0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [243] "153\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [244] "154\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [245] "155\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [246] "158\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [247] "159\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [248] "160\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [249] "162\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [250] "166\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [251] "167\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [252] "168\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [253] "171\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [254] "172\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [255] "173\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [256] "175\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [257] "178\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## 
## [[20]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/14BRNA2_S20_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/14BRNA2_S20_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/14BRNA2_S20_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/14BRNA2_S20_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##   [4] "Finished in 0.91 s (110 us/read; 0.54 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [8] "Total read pairs processed:              8,222"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##   [9] "  Read 1 with adapter:                     260 (3.2%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [10] "  Read 2 with adapter:                   1,543 (18.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [11] "Pairs written (passing filters):         8,219 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [13] "Total basepairs processed:     3,453,240 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [14] "  Read 1:     1,611,512 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [15] "  Read 2:     1,841,728 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [16] "Total written (filtered):      3,312,619 bp (95.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [17] "  Read 1:     1,594,065 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [18] "  Read 2:     1,718,554 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 12 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [29] "6\t2\t2.0\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [30] "17\t5\t0.0\t1\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [31] "18\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [32] "28\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [33] "31\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [34] "33\t1\t0.0\t3\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [35] "35\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [36] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [38] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [39] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [40] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 249 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [41] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [42] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [43] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [44] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [45] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [46] "  A: 58.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [47] "  C: 2.4%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [48] "  G: 32.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [49] "  T: 6.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [50] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [51] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [52] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [53] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [54] "5\t1\t8.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [55] "6\t1\t2.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [56] "8\t3\t0.1\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [57] "10\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [58] "11\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [59] "12\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [60] "13\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [61] "16\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [62] "19\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [63] "28\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [64] "29\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [65] "30\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [66] "36\t4\t0.0\t2\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [67] "37\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [68] "39\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [69] "42\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [70] "43\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [71] "45\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [72] "47\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [73] "52\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [74] "54\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [75] "55\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [76] "57\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [77] "58\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [78] "59\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [79] "60\t2\t0.0\t2\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [80] "61\t108\t0.0\t2\t104 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [81] "62\t9\t0.0\t2\t9"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [82] "71\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [83] "73\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [84] "74\t12\t0.0\t2\t12"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [85] "75\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [86] "77\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [87] "80\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [88] "81\t5\t0.0\t2\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [89] "82\t11\t0.0\t2\t10 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [90] "86\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [91] "88\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [92] "90\t38\t0.0\t2\t37 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [93] "91\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [94] "92\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [95] "95\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [96] "96\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [97] "104\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [98] "105\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [99] "108\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [100] "111\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [101] "123\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [102] "140\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [103] "143\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [104] "147\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [105] "152\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [106] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [107] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [108] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [109] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [110] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 17 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [111] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [112] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [113] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [114] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [115] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [116] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [117] "5\t2\t8.0\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [118] "6\t1\t2.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [119] "19\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [120] "29\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [121] "98\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [122] "123\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [123] "125\t10\t0.0\t2\t9 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [124] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [125] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [126] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [127] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [128] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 1528 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [129] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [130] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [131] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [132] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [133] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [134] "  A: 43.4%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [135] "  C: 8.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [136] "  G: 47.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [137] "  T: 0.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [138] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [139] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [140] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [141] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [142] "5\t3\t8.0\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [143] "6\t3\t2.0\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [144] "7\t7\t0.5\t0\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [145] "8\t11\t0.1\t0\t11"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [146] "9\t3\t0.0\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [147] "10\t4\t0.0\t1\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [148] "12\t8\t0.0\t1\t7 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [149] "13\t6\t0.0\t1\t4 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [150] "14\t12\t0.0\t1\t11 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [151] "15\t5\t0.0\t1\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [152] "16\t15\t0.0\t1\t12 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [153] "17\t7\t0.0\t1\t6 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [154] "18\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [155] "19\t3\t0.0\t1\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [156] "20\t16\t0.0\t2\t13 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [157] "21\t8\t0.0\t2\t6 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [158] "22\t6\t0.0\t2\t2 2 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [159] "23\t5\t0.0\t2\t3 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [160] "24\t5\t0.0\t2\t3 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [161] "25\t6\t0.0\t2\t5 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [162] "26\t9\t0.0\t2\t6 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [163] "27\t12\t0.0\t2\t7 5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [164] "28\t3\t0.0\t2\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [165] "30\t2\t0.0\t3\t0 1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [166] "31\t9\t0.0\t3\t4 2 1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [167] "32\t3\t0.0\t3\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [168] "33\t5\t0.0\t3\t3 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [169] "34\t11\t0.0\t3\t9 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [170] "35\t8\t0.0\t3\t7 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [171] "36\t5\t0.0\t3\t2 1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [172] "37\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [173] "38\t6\t0.0\t3\t4 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [174] "39\t3\t0.0\t3\t0 1 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [175] "40\t8\t0.0\t4\t4 0 2 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [176] "41\t7\t0.0\t4\t5 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [177] "42\t9\t0.0\t4\t6 2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [178] "43\t12\t0.0\t4\t9 2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [179] "44\t9\t0.0\t4\t7 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [180] "45\t12\t0.0\t4\t10 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [181] "46\t15\t0.0\t4\t8 5 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [182] "47\t9\t0.0\t4\t5 3 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [183] "48\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [184] "49\t3\t0.0\t4\t1 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [185] "50\t12\t0.0\t4\t8 3 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [186] "51\t9\t0.0\t4\t5 3 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [187] "52\t15\t0.0\t4\t7 4 3 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [188] "53\t4\t0.0\t4\t3 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [189] "54\t8\t0.0\t4\t6 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [190] "55\t4\t0.0\t4\t1 2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [191] "56\t12\t0.0\t4\t9 2 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [192] "57\t18\t0.0\t4\t9 5 1 1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [193] "58\t11\t0.0\t4\t8 1 0 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [194] "59\t9\t0.0\t4\t7 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [195] "60\t13\t0.0\t4\t8 5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [196] "61\t11\t0.0\t4\t7 3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [197] "62\t21\t0.0\t4\t17 2 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [198] "63\t30\t0.0\t4\t24 3 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [199] "64\t43\t0.0\t4\t34 6 2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [200] "65\t26\t0.0\t4\t18 7 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [201] "66\t21\t0.0\t4\t16 3 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [202] "67\t18\t0.0\t4\t17 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [203] "68\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [204] "69\t5\t0.0\t4\t3 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [205] "70\t13\t0.0\t4\t11 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [206] "71\t8\t0.0\t4\t4 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [207] "72\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [208] "73\t11\t0.0\t4\t9 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [209] "74\t24\t0.0\t4\t17 6 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [210] "75\t12\t0.0\t4\t10 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [211] "76\t7\t0.0\t4\t5 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [212] "77\t40\t0.0\t4\t34 5 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [213] "78\t11\t0.0\t4\t8 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [214] "79\t11\t0.0\t4\t7 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [215] "80\t5\t0.0\t4\t3 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [216] "81\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [217] "82\t11\t0.0\t4\t7 3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [218] "83\t32\t0.0\t4\t30 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [219] "84\t50\t0.0\t4\t44 5 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [220] "85\t63\t0.0\t4\t52 9 0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [221] "86\t22\t0.0\t4\t12 8 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [222] "87\t13\t0.0\t4\t10 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [223] "88\t8\t0.0\t4\t5 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [224] "89\t119\t0.0\t4\t20 78 14 6 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [225] "90\t9\t0.0\t4\t4 5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [226] "91\t6\t0.0\t4\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [227] "92\t15\t0.0\t4\t13 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [228] "93\t12\t0.0\t4\t9 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [229] "94\t12\t0.0\t4\t8 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [230] "95\t8\t0.0\t4\t7 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [231] "96\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [232] "97\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [233] "98\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [234] "99\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [235] "100\t6\t0.0\t4\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [236] "101\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [237] "102\t16\t0.0\t4\t16"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [238] "103\t8\t0.0\t4\t7 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [239] "104\t7\t0.0\t4\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [240] "105\t6\t0.0\t4\t2 2 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [241] "106\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [242] "107\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [243] "108\t8\t0.0\t4\t5 2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [244] "109\t12\t0.0\t4\t4 8"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [245] "110\t15\t0.0\t4\t7 7 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [246] "111\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [247] "112\t9\t0.0\t4\t8 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [248] "113\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [249] "114\t13\t0.0\t4\t12 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [250] "115\t9\t0.0\t4\t8 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [251] "116\t7\t0.0\t4\t5 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [252] "117\t6\t0.0\t4\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [253] "118\t43\t0.0\t4\t20 21 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [254] "119\t14\t0.0\t4\t10 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [255] "120\t10\t0.0\t4\t7 2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [256] "121\t7\t0.0\t4\t6 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [257] "122\t6\t0.0\t4\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [258] "123\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [259] "124\t9\t0.0\t4\t9"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [260] "125\t8\t0.0\t4\t7 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [261] "126\t10\t0.0\t4\t9 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [262] "127\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [263] "128\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [264] "129\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [265] "130\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [266] "131\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [267] "132\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [268] "133\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [269] "134\t3\t0.0\t4\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [270] "135\t7\t0.0\t4\t6 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [271] "136\t3\t0.0\t4\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [272] "137\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [273] "138\t9\t0.0\t4\t9"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [274] "139\t6\t0.0\t4\t3 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [275] "140\t18\t0.0\t4\t16 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [276] "141\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [277] "142\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [278] "143\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [279] "145\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [280] "146\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [281] "147\t6\t0.0\t4\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [282] "148\t8\t0.0\t4\t8"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [283] "149\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [284] "150\t6\t0.0\t4\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [285] "151\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [286] "152\t3\t0.0\t4\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [287] "153\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [288] "154\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [289] "157\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [290] "159\t3\t0.0\t4\t1 1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [291] "160\t7\t0.0\t4\t5 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [292] "161\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [293] "162\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [294] "163\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [295] "164\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [296] "165\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [297] "166\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [298] "167\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [299] "169\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [300] "170\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [301] "171\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [302] "172\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [303] "173\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [304] "175\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [305] "176\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [306] "180\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## 
## [[21]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/14BRNA3_S21_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/14BRNA3_S21_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/14BRNA3_S21_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/14BRNA3_S21_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##   [4] "Finished in 0.95 s (110 us/read; 0.54 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [8] "Total read pairs processed:              8,665"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##   [9] "  Read 1 with adapter:                     185 (2.1%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [10] "  Read 2 with adapter:                   1,055 (12.2%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [11] "Pairs written (passing filters):         8,663 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [13] "Total basepairs processed:     3,639,300 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [14] "  Read 1:     1,698,340 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [15] "  Read 2:     1,940,960 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [16] "Total written (filtered):      3,544,949 bp (97.4%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [17] "  Read 1:     1,687,020 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [18] "  Read 2:     1,857,929 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 20 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [29] "5\t1\t8.5\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [30] "8\t1\t0.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [31] "16\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [32] "17\t4\t0.0\t1\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [33] "31\t4\t0.0\t3\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [34] "32\t1\t0.0\t3\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [35] "33\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [36] "34\t4\t0.0\t3\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [37] "36\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [39] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [40] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [41] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [42] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 165 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [43] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [44] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [45] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [46] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [47] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [48] "  A: 71.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [49] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [50] "  G: 24.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [51] "  T: 3.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [52] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [53] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [54] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [55] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [56] "5\t2\t8.5\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [57] "6\t1\t2.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [58] "8\t1\t0.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [59] "13\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [60] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [61] "24\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [62] "29\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [63] "36\t4\t0.0\t2\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [64] "40\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [65] "43\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [66] "48\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [67] "54\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [68] "55\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [69] "56\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [70] "59\t5\t0.0\t2\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [71] "60\t5\t0.0\t2\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [72] "61\t98\t0.0\t2\t92 5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [73] "62\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [74] "65\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [75] "74\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [76] "80\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [77] "81\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [78] "82\t4\t0.0\t2\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [79] "90\t18\t0.0\t2\t17 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [80] "96\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [81] "98\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [82] "106\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [83] "108\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [84] "111\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [85] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [86] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [87] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [88] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [89] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 7 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [90] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [91] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [92] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [93] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [94] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [95] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [96] "5\t1\t8.5\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [97] "15\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [98] "76\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [99] "80\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [100] "125\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [101] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [102] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [103] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [104] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [105] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 1049 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [106] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [107] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [108] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [109] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [110] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [111] "  A: 43.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [112] "  C: 8.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [113] "  G: 47.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [114] "  T: 0.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [115] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [116] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [117] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [118] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [119] "5\t5\t8.5\t0\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [120] "6\t6\t2.1\t0\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [121] "7\t4\t0.5\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [122] "8\t9\t0.1\t0\t9"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [123] "9\t6\t0.0\t0\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [124] "10\t4\t0.0\t1\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [125] "11\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [126] "12\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [127] "13\t5\t0.0\t1\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [128] "14\t11\t0.0\t1\t9 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [129] "15\t8\t0.0\t1\t7 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [130] "16\t11\t0.0\t1\t10 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [131] "17\t5\t0.0\t1\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [132] "18\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [133] "19\t3\t0.0\t1\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [134] "20\t10\t0.0\t2\t7 2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [135] "21\t3\t0.0\t2\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [136] "22\t9\t0.0\t2\t6 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [137] "23\t2\t0.0\t2\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [138] "24\t3\t0.0\t2\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [139] "25\t2\t0.0\t2\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [140] "26\t4\t0.0\t2\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [141] "27\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [142] "28\t4\t0.0\t2\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [143] "29\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [144] "30\t7\t0.0\t3\t4 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [145] "31\t7\t0.0\t3\t4 2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [146] "32\t6\t0.0\t3\t4 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [147] "33\t6\t0.0\t3\t3 2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [148] "34\t5\t0.0\t3\t3 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [149] "35\t7\t0.0\t3\t5 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [150] "36\t6\t0.0\t3\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [151] "37\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [152] "38\t3\t0.0\t3\t1 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [153] "39\t3\t0.0\t3\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [154] "40\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [155] "41\t10\t0.0\t4\t4 1 4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [156] "42\t6\t0.0\t4\t3 1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [157] "43\t14\t0.0\t4\t7 5 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [158] "44\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [159] "45\t12\t0.0\t4\t5 5 1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [160] "46\t14\t0.0\t4\t8 5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [161] "47\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [162] "48\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [163] "49\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [164] "50\t9\t0.0\t4\t5 3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [165] "51\t4\t0.0\t4\t0 3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [166] "52\t11\t0.0\t4\t5 5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [167] "53\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [168] "54\t7\t0.0\t4\t5 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [169] "55\t7\t0.0\t4\t5 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [170] "56\t7\t0.0\t4\t5 1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [171] "57\t6\t0.0\t4\t4 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [172] "58\t6\t0.0\t4\t4 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [173] "59\t7\t0.0\t4\t6 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [174] "60\t5\t0.0\t4\t4 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [175] "61\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [176] "62\t14\t0.0\t4\t12 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [177] "63\t10\t0.0\t4\t7 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [178] "64\t23\t0.0\t4\t16 6 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [179] "65\t16\t0.0\t4\t12 1 1 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [180] "66\t19\t0.0\t4\t16 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [181] "67\t8\t0.0\t4\t5 1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [182] "68\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [183] "69\t4\t0.0\t4\t2 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [184] "71\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [185] "72\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [186] "73\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [187] "74\t9\t0.0\t4\t6 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [188] "75\t7\t0.0\t4\t5 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [189] "76\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [190] "77\t14\t0.0\t4\t10 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [191] "78\t9\t0.0\t4\t8 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [192] "79\t9\t0.0\t4\t7 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [193] "80\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [194] "81\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [195] "82\t8\t0.0\t4\t7 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [196] "83\t29\t0.0\t4\t25 3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [197] "84\t30\t0.0\t4\t28 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [198] "85\t30\t0.0\t4\t26 3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [199] "86\t16\t0.0\t4\t12 3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [200] "87\t13\t0.0\t4\t6 5 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [201] "88\t7\t0.0\t4\t3 3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [202] "89\t101\t0.0\t4\t13 73 13 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [203] "90\t7\t0.0\t4\t6 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [204] "91\t7\t0.0\t4\t5 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [205] "92\t8\t0.0\t4\t4 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [206] "93\t7\t0.0\t4\t5 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [207] "94\t13\t0.0\t4\t8 5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [208] "95\t8\t0.0\t4\t3 4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [209] "96\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [210] "97\t4\t0.0\t4\t3 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [211] "98\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [212] "99\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [213] "100\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [214] "101\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [215] "102\t10\t0.0\t4\t10"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [216] "103\t8\t0.0\t4\t7 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [217] "104\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [218] "105\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [219] "106\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [220] "108\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [221] "109\t7\t0.0\t4\t6 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [222] "110\t13\t0.0\t4\t8 5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [223] "111\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [224] "112\t10\t0.0\t4\t6 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [225] "113\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [226] "114\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [227] "115\t7\t0.0\t4\t6 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [228] "116\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [229] "117\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [230] "118\t20\t0.0\t4\t12 8"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [231] "119\t12\t0.0\t4\t11 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [232] "120\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [233] "121\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [234] "122\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [235] "123\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [236] "124\t4\t0.0\t4\t2 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [237] "125\t5\t0.0\t4\t2 2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [238] "126\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [239] "127\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [240] "128\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [241] "129\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [242] "130\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [243] "131\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [244] "132\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [245] "133\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [246] "134\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [247] "135\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [248] "136\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [249] "137\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [250] "138\t8\t0.0\t4\t6 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [251] "139\t8\t0.0\t4\t7 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [252] "140\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [253] "141\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [254] "142\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [255] "143\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [256] "144\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [257] "145\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [258] "146\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [259] "147\t3\t0.0\t4\t1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [260] "148\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [261] "149\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [262] "150\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [263] "151\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [264] "152\t2\t0.0\t4\t0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [265] "153\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [266] "154\t4\t0.0\t4\t3 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [267] "155\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [268] "158\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [269] "160\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [270] "161\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [271] "162\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [272] "163\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [273] "164\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [274] "165\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [275] "166\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [276] "172\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [277] "174\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [278] "177\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [279] "179\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## 
## [[22]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/14BRNA4_S22_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/14BRNA4_S22_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/14BRNA4_S22_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/14BRNA4_S22_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##   [4] "Finished in 0.46 s (111 us/read; 0.54 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [8] "Total read pairs processed:              4,121"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##   [9] "  Read 1 with adapter:                      34 (0.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [10] "  Read 2 with adapter:                     141 (3.4%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [11] "Pairs written (passing filters):         4,119 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [13] "Total basepairs processed:     1,730,820 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [14] "  Read 1:       807,716 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [15] "  Read 2:       923,104 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [16] "Total written (filtered):      1,718,599 bp (99.3%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [17] "  Read 1:       805,626 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [18] "  Read 2:       912,973 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [29] "31\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [30] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [32] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [34] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 33 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [36] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [37] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [39] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [40] "  A: 66.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [41] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [42] "  G: 33.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [43] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [44] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [45] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [46] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [47] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [48] "5\t3\t4.0\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [49] "8\t2\t0.1\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [50] "10\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [51] "12\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [52] "27\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [53] "30\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [54] "31\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [55] "34\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [56] "43\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [57] "45\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [58] "55\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [59] "61\t14\t0.0\t2\t14"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [60] "71\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [61] "73\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [62] "90\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [63] "116\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [64] "145\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [65] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [66] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [67] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [68] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [69] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [70] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [71] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [72] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [73] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 141 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [74] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [75] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [76] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [77] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [78] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [79] "  A: 53.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [80] "  C: 11.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [81] "  G: 35.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [82] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [83] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [84] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [85] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [86] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [87] "5\t1\t4.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [88] "6\t1\t1.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [89] "8\t2\t0.1\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [90] "10\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [91] "13\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [92] "14\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [93] "15\t2\t0.0\t1\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [94] "16\t2\t0.0\t1\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [95] "20\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [96] "22\t3\t0.0\t2\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [97] "27\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [98] "30\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [99] "31\t2\t0.0\t3\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [100] "33\t4\t0.0\t3\t2 1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [101] "34\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [102] "35\t3\t0.0\t3\t1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [103] "36\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [104] "38\t2\t0.0\t3\t1 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [105] "39\t3\t0.0\t3\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [106] "40\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [107] "41\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [108] "42\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [109] "43\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [110] "44\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [111] "45\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [112] "46\t2\t0.0\t4\t0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [113] "47\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [114] "51\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [115] "52\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [116] "53\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [117] "54\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [118] "56\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [119] "57\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [120] "58\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [121] "59\t5\t0.0\t4\t3 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [122] "60\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [123] "62\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [124] "63\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [125] "65\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [126] "66\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [127] "70\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [128] "71\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [129] "72\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [130] "73\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [131] "75\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [132] "77\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [133] "78\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [134] "79\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [135] "80\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [136] "82\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [137] "83\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [138] "85\t2\t0.0\t4\t1 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [139] "89\t15\t0.0\t4\t0 12 2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [140] "91\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [141] "92\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [142] "94\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [143] "97\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [144] "101\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [145] "102\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [146] "103\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [147] "105\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [148] "106\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [149] "108\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [150] "110\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [151] "111\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [152] "115\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [153] "116\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [154] "117\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [155] "118\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [156] "127\t2\t0.0\t4\t1 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [157] "130\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [158] "134\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [159] "141\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [160] "144\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [161] "148\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [162] "149\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [163] "150\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [164] "160\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [165] "173\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [166] "180\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [167] "194\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## 
## [[23]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/14C1_S23_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/14C1_S23_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/14C1_S23_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/14C1_S23_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##   [4] "Finished in 1.26 s (106 us/read; 0.57 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [8] "Total read pairs processed:             11,911"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [9] "  Read 1 with adapter:                       5 (0.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [10] "  Read 2 with adapter:                     187 (1.6%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [11] "Pairs written (passing filters):        11,911 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [13] "Total basepairs processed:     5,002,620 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [14] "  Read 1:     2,334,556 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [15] "  Read 2:     2,668,064 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [16] "Total written (filtered):      4,990,066 bp (99.7%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [17] "  Read 1:     2,334,490 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [18] "  Read 2:     2,655,576 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 5 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [29] "5\t1\t11.6\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [30] "6\t1\t2.9\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [31] "7\t1\t0.7\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [32] "22\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [33] "26\t1\t0.0\t2\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [34] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [36] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [38] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [39] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [40] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [41] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [42] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [43] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [44] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [45] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [46] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 187 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [47] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [48] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [49] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [50] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [51] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [52] "  A: 66.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [53] "  C: 11.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [54] "  G: 22.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [55] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [56] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [57] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [58] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [59] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [60] "5\t7\t11.6\t0\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [61] "6\t1\t2.9\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [62] "7\t1\t0.7\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [63] "8\t4\t0.2\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [64] "9\t1\t0.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [65] "10\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [66] "11\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [67] "12\t2\t0.0\t1\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [68] "13\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [69] "14\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [70] "15\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [71] "16\t5\t0.0\t1\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [72] "17\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [73] "18\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [74] "22\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [75] "23\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [76] "24\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [77] "25\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [78] "27\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [79] "30\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [80] "31\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [81] "33\t4\t0.0\t3\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [82] "34\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [83] "35\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [84] "36\t4\t0.0\t3\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [85] "37\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [86] "39\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [87] "40\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [88] "42\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [89] "43\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [90] "44\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [91] "45\t3\t0.0\t4\t1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [92] "46\t2\t0.0\t4\t0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [93] "47\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [94] "50\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [95] "51\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [96] "52\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [97] "55\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [98] "56\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [99] "57\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [100] "60\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [101] "62\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [102] "63\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [103] "64\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [104] "65\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [105] "67\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [106] "69\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [107] "70\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [108] "72\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [109] "73\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [110] "74\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [111] "76\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [112] "77\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [113] "78\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [114] "80\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [115] "81\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [116] "82\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [117] "83\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [118] "84\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [119] "85\t2\t0.0\t4\t0 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [120] "86\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [121] "87\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [122] "89\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [123] "90\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [124] "92\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [125] "93\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [126] "94\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [127] "95\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [128] "97\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [129] "100\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [130] "101\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [131] "102\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [132] "103\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [133] "104\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [134] "105\t6\t0.0\t4\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [135] "106\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [136] "112\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [137] "113\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [138] "114\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [139] "116\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [140] "117\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [141] "119\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [142] "122\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [143] "124\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [144] "125\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [145] "126\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [146] "127\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [147] "128\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [148] "138\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [149] "139\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [150] "140\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [151] "146\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [152] "147\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [153] "148\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [154] "149\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [155] "160\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [156] "161\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## 
## [[24]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/14C2_S24_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/14C2_S24_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/14C2_S24_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/14C2_S24_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##   [4] "Finished in 1.48 s (105 us/read; 0.57 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [8] "Total read pairs processed:             14,079"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [9] "  Read 1 with adapter:                       8 (0.1%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [10] "  Read 2 with adapter:                     217 (1.5%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [11] "Pairs written (passing filters):        14,079 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [13] "Total basepairs processed:     5,913,180 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [14] "  Read 1:     2,759,484 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [15] "  Read 2:     3,153,696 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [16] "Total written (filtered):      5,899,961 bp (99.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [17] "  Read 1:     2,759,402 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [18] "  Read 2:     3,140,559 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 7 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [29] "5\t1\t13.7\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [30] "6\t1\t3.4\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [31] "7\t1\t0.9\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [32] "8\t1\t0.2\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [33] "15\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [34] "16\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [35] "20\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [36] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [38] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [39] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [40] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [41] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [42] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [43] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [44] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [45] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [46] "  A: 100.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [47] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [48] "  G: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [49] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [50] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [51] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [52] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [53] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [54] "5\t1\t13.7\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [55] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [56] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [57] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [58] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [59] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [60] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [61] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [62] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [63] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [64] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [65] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [66] "22\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [67] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [68] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [69] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [70] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [71] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 216 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [72] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [73] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [74] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [75] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [76] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [77] "  A: 48.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [78] "  C: 30.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [79] "  G: 21.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [80] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [81] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [82] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [83] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [84] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [85] "6\t2\t3.4\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [86] "7\t2\t0.9\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [87] "8\t5\t0.2\t0\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [88] "9\t2\t0.1\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [89] "12\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [90] "13\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [91] "14\t9\t0.0\t1\t8 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [92] "16\t12\t0.0\t1\t10 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [93] "17\t4\t0.0\t1\t2 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [94] "18\t4\t0.0\t1\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [95] "19\t4\t0.0\t1\t0 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [96] "20\t2\t0.0\t2\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [97] "21\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [98] "22\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [99] "25\t5\t0.0\t2\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [100] "26\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [101] "27\t1\t0.0\t2\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [102] "28\t3\t0.0\t2\t1 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [103] "29\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [104] "30\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [105] "31\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [106] "32\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [107] "33\t3\t0.0\t3\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [108] "34\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [109] "35\t2\t0.0\t3\t0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [110] "39\t4\t0.0\t3\t2 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [111] "40\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [112] "41\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [113] "42\t3\t0.0\t4\t2 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [114] "44\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [115] "45\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [116] "46\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [117] "47\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [118] "48\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [119] "50\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [120] "51\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [121] "52\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [122] "53\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [123] "54\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [124] "55\t2\t0.0\t4\t1 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [125] "56\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [126] "58\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [127] "60\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [128] "62\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [129] "63\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [130] "64\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [131] "65\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [132] "66\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [133] "67\t3\t0.0\t4\t2 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [134] "68\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [135] "72\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [136] "73\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [137] "74\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [138] "75\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [139] "76\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [140] "77\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [141] "79\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [142] "80\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [143] "82\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [144] "83\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [145] "84\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [146] "85\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [147] "86\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [148] "87\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [149] "88\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [150] "90\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [151] "93\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [152] "94\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [153] "98\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [154] "101\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [155] "102\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [156] "103\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [157] "104\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [158] "105\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [159] "106\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [160] "107\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [161] "109\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [162] "111\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [163] "112\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [164] "113\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [165] "118\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [166] "119\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [167] "120\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [168] "123\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [169] "124\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [170] "125\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [171] "126\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [172] "129\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [173] "134\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [174] "137\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [175] "138\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [176] "139\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [177] "140\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [178] "142\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [179] "147\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [180] "148\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [181] "151\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [182] "167\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [183] "169\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## 
## [[25]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/14C3_S25_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/14C3_S25_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/14C3_S25_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/14C3_S25_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##   [4] "Finished in 1.02 s (110 us/read; 0.55 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [8] "Total read pairs processed:              9,283"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [9] "  Read 1 with adapter:                      42 (0.5%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [10] "  Read 2 with adapter:                     355 (3.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [11] "Pairs written (passing filters):         9,282 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [13] "Total basepairs processed:     3,898,860 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [14] "  Read 1:     1,819,468 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [15] "  Read 2:     2,079,392 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [16] "Total written (filtered):      3,873,183 bp (99.3%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [17] "  Read 1:     1,818,283 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [18] "  Read 2:     2,054,900 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 40 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [29] "7\t1\t0.6\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [30] "16\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [31] "17\t27\t0.0\t1\t27"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [32] "30\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [33] "31\t7\t0.0\t3\t6 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [34] "48\t2\t0.0\t4\t0 0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [35] "71\t1\t0.0\t4\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [36] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [38] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [39] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [40] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 2 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [41] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [42] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [43] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [44] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [45] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [46] "  A: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [47] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [48] "  G: 100.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [49] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [50] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [51] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [52] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [53] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [54] "72\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [55] "92\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [56] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [57] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [58] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [59] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [60] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [61] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [62] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [63] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [64] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [65] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [66] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [67] "31\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [68] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [69] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [70] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [71] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [72] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 354 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [73] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [74] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [75] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [76] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [77] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [78] "  A: 56.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [79] "  C: 12.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [80] "  G: 30.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [81] "  T: 0.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [82] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [83] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [84] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [85] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [86] "5\t2\t9.1\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [87] "6\t2\t2.3\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [88] "7\t4\t0.6\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [89] "8\t1\t0.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [90] "9\t1\t0.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [91] "11\t2\t0.0\t1\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [92] "12\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [93] "13\t4\t0.0\t1\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [94] "14\t6\t0.0\t1\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [95] "15\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [96] "16\t7\t0.0\t1\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [97] "17\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [98] "18\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [99] "19\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [100] "20\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [101] "21\t4\t0.0\t2\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [102] "22\t7\t0.0\t2\t6 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [103] "23\t4\t0.0\t2\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [104] "24\t4\t0.0\t2\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [105] "25\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [106] "26\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [107] "27\t3\t0.0\t2\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [108] "28\t2\t0.0\t2\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [109] "30\t2\t0.0\t3\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [110] "31\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [111] "32\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [112] "33\t3\t0.0\t3\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [113] "34\t3\t0.0\t3\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [114] "35\t4\t0.0\t3\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [115] "36\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [116] "37\t1\t0.0\t3\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [117] "38\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [118] "39\t5\t0.0\t3\t2 2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [119] "40\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [120] "41\t2\t0.0\t4\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [121] "42\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [122] "43\t5\t0.0\t4\t3 1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [123] "44\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [124] "45\t4\t0.0\t4\t2 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [125] "46\t6\t0.0\t4\t2 3 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [126] "47\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [127] "49\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [128] "50\t4\t0.0\t4\t2 1 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [129] "51\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [130] "52\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [131] "53\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [132] "54\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [133] "55\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [134] "56\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [135] "57\t6\t0.0\t4\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [136] "58\t4\t0.0\t4\t3 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [137] "59\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [138] "60\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [139] "61\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [140] "62\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [141] "63\t6\t0.0\t4\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [142] "64\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [143] "65\t5\t0.0\t4\t3 1 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [144] "66\t7\t0.0\t4\t6 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [145] "67\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [146] "68\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [147] "69\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [148] "70\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [149] "72\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [150] "73\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [151] "74\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [152] "75\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [153] "76\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [154] "77\t4\t0.0\t4\t2 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [155] "78\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [156] "79\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [157] "80\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [158] "81\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [159] "82\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [160] "83\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [161] "84\t8\t0.0\t4\t7 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [162] "85\t6\t0.0\t4\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [163] "86\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [164] "87\t3\t0.0\t4\t2 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [165] "88\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [166] "89\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [167] "92\t5\t0.0\t4\t2 2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [168] "94\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [169] "95\t7\t0.0\t4\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [170] "96\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [171] "97\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [172] "98\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [173] "99\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [174] "101\t1\t0.0\t4\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [175] "102\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [176] "103\t1\t0.0\t4\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [177] "104\t1\t0.0\t4\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [178] "105\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [179] "106\t7\t0.0\t4\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [180] "108\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [181] "109\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [182] "110\t1\t0.0\t4\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [183] "112\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [184] "113\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [185] "115\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [186] "116\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [187] "117\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [188] "118\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [189] "120\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [190] "121\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [191] "122\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [192] "123\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [193] "125\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [194] "126\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [195] "128\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [196] "131\t3\t0.0\t4\t2 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [197] "132\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [198] "133\t1\t0.0\t4\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [199] "134\t3\t0.0\t4\t2 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [200] "135\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [201] "137\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [202] "139\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [203] "141\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [204] "144\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [205] "145\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [206] "146\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [207] "148\t2\t0.0\t4\t1 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [208] "152\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [209] "154\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [210] "159\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [211] "160\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [212] "161\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [213] "162\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [214] "163\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [215] "166\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [216] "174\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [217] "210\t1\t0.0\t4\t0 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## 
## [[26]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/14CRNA1_S26_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/14CRNA1_S26_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/14CRNA1_S26_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/14CRNA1_S26_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##   [4] "Finished in 0.57 s (108 us/read; 0.56 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [8] "Total read pairs processed:              5,310"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##   [9] "  Read 1 with adapter:                      22 (0.4%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [10] "  Read 2 with adapter:                     243 (4.6%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [11] "Pairs written (passing filters):         5,310 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [13] "Total basepairs processed:     2,230,200 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [14] "  Read 1:     1,040,760 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [15] "  Read 2:     1,189,440 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [16] "Total written (filtered):      2,211,302 bp (99.2%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [17] "  Read 1:     1,039,566 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [18] "  Read 2:     1,171,736 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 3 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [29] "7\t1\t0.3\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [30] "31\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [31] "33\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [32] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [34] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [36] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 19 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [38] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [39] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [40] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [41] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [42] "  A: 31.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [43] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [44] "  G: 68.4%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [45] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [46] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [47] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [48] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [49] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [50] "8\t1\t0.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [51] "14\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [52] "15\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [53] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [54] "36\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [55] "61\t5\t0.0\t2\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [56] "62\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [57] "67\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [58] "74\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [59] "90\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [60] "107\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [61] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [62] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [63] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [64] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [65] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 2 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [66] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [67] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [68] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [69] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [70] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [71] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [72] "12\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [73] "125\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [74] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [75] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [76] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [77] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [78] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 241 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [79] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [80] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [81] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [82] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [83] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [84] "  A: 22.4%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [85] "  C: 10.4%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [86] "  G: 67.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [87] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [88] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [89] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [90] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [91] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [92] "5\t1\t5.2\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [93] "6\t5\t1.3\t0\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [94] "7\t2\t0.3\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [95] "8\t3\t0.1\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [96] "10\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [97] "13\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [98] "15\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [99] "16\t4\t0.0\t1\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [100] "18\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [101] "19\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [102] "20\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [103] "21\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [104] "22\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [105] "24\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [106] "25\t2\t0.0\t2\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [107] "26\t4\t0.0\t2\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [108] "27\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [109] "28\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [110] "30\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [111] "31\t3\t0.0\t3\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [112] "32\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [113] "33\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [114] "36\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [115] "40\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [116] "41\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [117] "42\t2\t0.0\t4\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [118] "43\t4\t0.0\t4\t2 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [119] "45\t5\t0.0\t4\t3 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [120] "46\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [121] "47\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [122] "48\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [123] "49\t4\t0.0\t4\t2 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [124] "50\t3\t0.0\t4\t2 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [125] "51\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [126] "52\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [127] "53\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [128] "56\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [129] "57\t6\t0.0\t4\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [130] "59\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [131] "60\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [132] "61\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [133] "62\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [134] "63\t7\t0.0\t4\t6 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [135] "64\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [136] "65\t3\t0.0\t4\t1 1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [137] "66\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [138] "67\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [139] "68\t4\t0.0\t4\t3 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [140] "69\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [141] "70\t1\t0.0\t4\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [142] "72\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [143] "73\t3\t0.0\t4\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [144] "74\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [145] "75\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [146] "77\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [147] "78\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [148] "79\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [149] "80\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [150] "83\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [151] "84\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [152] "85\t12\t0.0\t4\t10 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [153] "86\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [154] "88\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [155] "89\t6\t0.0\t4\t1 4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [156] "90\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [157] "91\t2\t0.0\t4\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [158] "92\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [159] "93\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [160] "94\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [161] "95\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [162] "96\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [163] "98\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [164] "101\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [165] "102\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [166] "103\t4\t0.0\t4\t2 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [167] "104\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [168] "105\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [169] "106\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [170] "112\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [171] "113\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [172] "115\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [173] "117\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [174] "118\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [175] "119\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [176] "120\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [177] "121\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [178] "123\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [179] "125\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [180] "126\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [181] "130\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [182] "134\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [183] "135\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [184] "137\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [185] "138\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [186] "140\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [187] "141\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [188] "143\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [189] "144\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [190] "146\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [191] "149\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [192] "160\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [193] "161\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [194] "162\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [195] "163\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## 
## [[27]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/14CRNA2_S27_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/14CRNA2_S27_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/14CRNA2_S27_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/14CRNA2_S27_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##   [4] "Finished in 0.74 s (111 us/read; 0.54 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [8] "Total read pairs processed:              6,680"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##   [9] "  Read 1 with adapter:                     186 (2.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [10] "  Read 2 with adapter:                   1,031 (15.4%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [11] "Pairs written (passing filters):         6,676 (99.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [13] "Total basepairs processed:     2,805,600 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [14] "  Read 1:     1,309,280 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [15] "  Read 2:     1,496,320 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [16] "Total written (filtered):      2,714,208 bp (96.7%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [17] "  Read 1:     1,297,036 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [18] "  Read 2:     1,417,172 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 5 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [29] "33\t2\t0.0\t3\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [30] "34\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [31] "36\t1\t0.0\t3\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [32] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [34] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [36] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 181 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [38] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [39] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [40] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [41] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [42] "  A: 84.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [43] "  C: 0.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [44] "  G: 13.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [45] "  T: 1.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [46] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [47] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [48] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [49] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [50] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [51] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [52] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [53] "5\t1\t6.5\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [54] "7\t1\t0.4\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [55] "15\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [56] "26\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [57] "28\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [58] "29\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [59] "30\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [60] "33\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [61] "36\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [62] "54\t7\t0.0\t2\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [63] "55\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [64] "59\t2\t0.0\t2\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [65] "60\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [66] "61\t126\t0.0\t2\t122 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [67] "62\t4\t0.0\t2\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [68] "63\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [69] "67\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [70] "69\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [71] "71\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [72] "74\t5\t0.0\t2\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [73] "82\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [74] "90\t10\t0.0\t2\t10"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [75] "91\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [76] "96\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [77] "99\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [78] "123\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [79] "135\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [80] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [81] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [82] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [83] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [84] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [85] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [86] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [87] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [88] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [89] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [90] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [91] "16\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [92] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [93] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [94] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [95] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [96] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 1030 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [97] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [98] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [99] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [100] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [101] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [102] "  A: 53.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [103] "  C: 13.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [104] "  G: 32.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [105] "  T: 0.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [106] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [107] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [108] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [109] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [110] "5\t2\t6.5\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [111] "6\t4\t1.6\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [112] "7\t1\t0.4\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [113] "8\t10\t0.1\t0\t10"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [114] "9\t3\t0.0\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [115] "10\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [116] "11\t7\t0.0\t1\t6 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [117] "12\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [118] "13\t2\t0.0\t1\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [119] "14\t10\t0.0\t1\t7 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [120] "15\t5\t0.0\t1\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [121] "16\t12\t0.0\t1\t11 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [122] "18\t6\t0.0\t1\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [123] "19\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [124] "20\t6\t0.0\t2\t4 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [125] "21\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [126] "22\t9\t0.0\t2\t7 0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [127] "23\t6\t0.0\t2\t3 2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [128] "24\t5\t0.0\t2\t3 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [129] "25\t4\t0.0\t2\t1 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [130] "26\t6\t0.0\t2\t4 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [131] "27\t7\t0.0\t2\t4 2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [132] "28\t3\t0.0\t2\t1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [133] "29\t4\t0.0\t2\t3 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [134] "30\t6\t0.0\t3\t1 5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [135] "31\t7\t0.0\t3\t2 4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [136] "32\t5\t0.0\t3\t3 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [137] "33\t5\t0.0\t3\t3 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [138] "34\t3\t0.0\t3\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [139] "35\t12\t0.0\t3\t9 2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [140] "36\t7\t0.0\t3\t6 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [141] "38\t4\t0.0\t3\t2 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [142] "39\t8\t0.0\t3\t4 2 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [143] "40\t7\t0.0\t4\t4 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [144] "41\t10\t0.0\t4\t3 6 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [145] "42\t6\t0.0\t4\t2 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [146] "43\t9\t0.0\t4\t7 1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [147] "44\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [148] "45\t16\t0.0\t4\t12 3 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [149] "46\t13\t0.0\t4\t8 5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [150] "47\t6\t0.0\t4\t4 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [151] "48\t3\t0.0\t4\t1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [152] "49\t6\t0.0\t4\t3 1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [153] "50\t7\t0.0\t4\t5 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [154] "51\t11\t0.0\t4\t8 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [155] "52\t15\t0.0\t4\t6 9"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [156] "53\t4\t0.0\t4\t2 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [157] "54\t5\t0.0\t4\t3 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [158] "55\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [159] "56\t9\t0.0\t4\t5 2 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [160] "57\t9\t0.0\t4\t7 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [161] "58\t9\t0.0\t4\t6 2 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [162] "59\t6\t0.0\t4\t2 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [163] "60\t20\t0.0\t4\t17 2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [164] "61\t4\t0.0\t4\t2 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [165] "62\t8\t0.0\t4\t4 3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [166] "63\t13\t0.0\t4\t11 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [167] "64\t16\t0.0\t4\t13 1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [168] "65\t10\t0.0\t4\t7 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [169] "66\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [170] "67\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [171] "68\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [172] "69\t9\t0.0\t4\t8 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [173] "70\t9\t0.0\t4\t6 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [174] "71\t4\t0.0\t4\t2 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [175] "72\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [176] "73\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [177] "74\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [178] "75\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [179] "76\t3\t0.0\t4\t1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [180] "77\t9\t0.0\t4\t6 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [181] "78\t7\t0.0\t4\t4 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [182] "79\t16\t0.0\t4\t9 7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [183] "80\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [184] "81\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [185] "82\t8\t0.0\t4\t0 5 2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [186] "83\t11\t0.0\t4\t7 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [187] "84\t18\t0.0\t4\t14 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [188] "85\t24\t0.0\t4\t17 6 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [189] "86\t15\t0.0\t4\t10 5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [190] "87\t7\t0.0\t4\t3 2 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [191] "88\t7\t0.0\t4\t2 3 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [192] "89\t135\t0.0\t4\t9 102 24"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [193] "90\t2\t0.0\t4\t0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [194] "91\t9\t0.0\t4\t5 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [195] "92\t12\t0.0\t4\t10 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [196] "93\t6\t0.0\t4\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [197] "94\t17\t0.0\t4\t13 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [198] "95\t11\t0.0\t4\t8 2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [199] "96\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [200] "97\t6\t0.0\t4\t3 2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [201] "98\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [202] "99\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [203] "100\t7\t0.0\t4\t5 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [204] "101\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [205] "102\t10\t0.0\t4\t10"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [206] "103\t7\t0.0\t4\t4 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [207] "104\t11\t0.0\t4\t10 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [208] "105\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [209] "106\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [210] "108\t4\t0.0\t4\t1 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [211] "109\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [212] "110\t5\t0.0\t4\t2 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [213] "111\t3\t0.0\t4\t1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [214] "112\t12\t0.0\t4\t7 4 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [215] "113\t5\t0.0\t4\t3 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [216] "114\t4\t0.0\t4\t2 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [217] "115\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [218] "116\t8\t0.0\t4\t6 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [219] "117\t7\t0.0\t4\t4 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [220] "118\t13\t0.0\t4\t4 8 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [221] "119\t14\t0.0\t4\t10 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [222] "120\t6\t0.0\t4\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [223] "121\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [224] "123\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [225] "124\t5\t0.0\t4\t4 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [226] "125\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [227] "126\t6\t0.0\t4\t4 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [228] "127\t4\t0.0\t4\t1 2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [229] "128\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [230] "129\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [231] "130\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [232] "131\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [233] "132\t5\t0.0\t4\t2 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [234] "133\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [235] "134\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [236] "135\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [237] "136\t3\t0.0\t4\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [238] "137\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [239] "138\t7\t0.0\t4\t5 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [240] "139\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [241] "140\t7\t0.0\t4\t6 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [242] "141\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [243] "142\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [244] "144\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [245] "145\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [246] "146\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [247] "147\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [248] "148\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [249] "149\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [250] "150\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [251] "151\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [252] "152\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [253] "153\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [254] "154\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [255] "158\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [256] "159\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [257] "160\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [258] "161\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [259] "162\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [260] "163\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [261] "165\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [262] "169\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [263] "171\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [264] "180\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [265] "188\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [266] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [267] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [268] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [269] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [270] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## 
## [[28]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/14CRNA3_S28_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/14CRNA3_S28_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/14CRNA3_S28_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/14CRNA3_S28_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##   [4] "Finished in 0.51 s (108 us/read; 0.55 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [8] "Total read pairs processed:              4,749"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##   [9] "  Read 1 with adapter:                      34 (0.7%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [10] "  Read 2 with adapter:                     369 (7.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [11] "Pairs written (passing filters):         4,749 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [13] "Total basepairs processed:     1,994,580 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [14] "  Read 1:       930,804 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [15] "  Read 2:     1,063,776 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [16] "Total written (filtered):      1,965,785 bp (98.6%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [17] "  Read 1:       929,307 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [18] "  Read 2:     1,036,478 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 15 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [29] "10\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [30] "11\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [31] "15\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [32] "17\t6\t0.0\t1\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [33] "20\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [34] "30\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [35] "31\t4\t0.0\t3\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [36] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [38] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [39] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [40] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 19 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [41] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [42] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [43] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [44] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [45] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [46] "  A: 52.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [47] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [48] "  G: 42.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [49] "  T: 5.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [50] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [51] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [52] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [53] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [54] "12\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [55] "21\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [56] "36\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [57] "61\t9\t0.0\t2\t8 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [58] "74\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [59] "82\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [60] "90\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [61] "91\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [62] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [63] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [64] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [65] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [66] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [67] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [68] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [69] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [70] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 369 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [71] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [72] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [73] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [74] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [75] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [76] "  A: 21.4%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [77] "  C: 7.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [78] "  G: 71.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [79] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [80] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [81] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [82] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [83] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [84] "5\t3\t4.6\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [85] "6\t2\t1.2\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [86] "7\t3\t0.3\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [87] "8\t3\t0.1\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [88] "9\t2\t0.0\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [89] "11\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [90] "12\t3\t0.0\t1\t1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [91] "13\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [92] "14\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [93] "15\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [94] "16\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [95] "17\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [96] "18\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [97] "19\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [98] "20\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [99] "21\t4\t0.0\t2\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [100] "22\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [101] "23\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [102] "24\t3\t0.0\t2\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [103] "25\t3\t0.0\t2\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [104] "26\t2\t0.0\t2\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [105] "27\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [106] "30\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [107] "31\t6\t0.0\t3\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [108] "32\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [109] "33\t5\t0.0\t3\t3 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [110] "34\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [111] "35\t6\t0.0\t3\t4 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [112] "36\t3\t0.0\t3\t2 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [113] "37\t2\t0.0\t3\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [114] "38\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [115] "39\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [116] "40\t5\t0.0\t4\t3 1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [117] "42\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [118] "43\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [119] "44\t5\t0.0\t4\t3 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [120] "45\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [121] "46\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [122] "47\t4\t0.0\t4\t3 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [123] "49\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [124] "50\t7\t0.0\t4\t5 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [125] "51\t8\t0.0\t4\t6 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [126] "52\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [127] "53\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [128] "54\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [129] "55\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [130] "56\t4\t0.0\t4\t2 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [131] "57\t2\t0.0\t4\t0 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [132] "59\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [133] "60\t8\t0.0\t4\t6 1 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [134] "61\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [135] "62\t3\t0.0\t4\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [136] "63\t7\t0.0\t4\t5 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [137] "64\t7\t0.0\t4\t6 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [138] "65\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [139] "66\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [140] "67\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [141] "69\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [142] "70\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [143] "71\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [144] "72\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [145] "73\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [146] "74\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [147] "75\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [148] "76\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [149] "77\t7\t0.0\t4\t5 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [150] "79\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [151] "80\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [152] "81\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [153] "82\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [154] "83\t8\t0.0\t4\t8"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [155] "84\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [156] "85\t11\t0.0\t4\t10 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [157] "86\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [158] "87\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [159] "88\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [160] "89\t9\t0.0\t4\t2 6 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [161] "90\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [162] "91\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [163] "92\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [164] "93\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [165] "94\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [166] "95\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [167] "96\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [168] "97\t3\t0.0\t4\t2 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [169] "98\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [170] "100\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [171] "101\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [172] "102\t6\t0.0\t4\t4 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [173] "103\t7\t0.0\t4\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [174] "104\t7\t0.0\t4\t5 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [175] "105\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [176] "106\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [177] "108\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [178] "109\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [179] "110\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [180] "112\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [181] "113\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [182] "115\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [183] "116\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [184] "117\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [185] "118\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [186] "119\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [187] "120\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [188] "121\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [189] "122\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [190] "123\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [191] "124\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [192] "125\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [193] "126\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [194] "131\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [195] "133\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [196] "135\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [197] "138\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [198] "139\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [199] "140\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [200] "141\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [201] "143\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [202] "144\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [203] "145\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [204] "148\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [205] "150\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [206] "152\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [207] "160\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [208] "162\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [209] "163\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [210] "165\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [211] "170\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [212] "173\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## 
## [[29]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/1881_S29_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/1881_S29_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/1881_S29_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/1881_S29_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##   [4] "Finished in 0.86 s (124 us/read; 0.49 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [8] "Total read pairs processed:              6,964"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [9] "  Read 1 with adapter:                      25 (0.4%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [10] "  Read 2 with adapter:                     346 (5.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [11] "Pairs written (passing filters):         6,964 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [13] "Total basepairs processed:     2,924,880 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [14] "  Read 1:     1,364,944 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [15] "  Read 2:     1,559,936 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [16] "Total written (filtered):      2,902,335 bp (99.2%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [17] "  Read 1:     1,364,349 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [18] "  Read 2:     1,537,986 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 19 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [29] "5\t1\t6.8\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [30] "6\t2\t1.7\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [31] "12\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [32] "16\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [33] "17\t7\t0.0\t1\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [34] "25\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [35] "31\t5\t0.0\t3\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [36] "56\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [39] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [40] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [41] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 6 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [42] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [43] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [44] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [45] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [46] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [47] "  A: 33.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [48] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [49] "  G: 50.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [50] "  T: 16.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [51] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [52] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [53] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [54] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [55] "6\t1\t1.7\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [56] "10\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [57] "19\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [58] "30\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [59] "120\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [60] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [61] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [62] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [63] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [64] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 3 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [65] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [66] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [67] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [68] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [69] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [70] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [71] "6\t1\t1.7\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [72] "16\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [73] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [74] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [75] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [76] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [77] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 343 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [78] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [79] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [80] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [81] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [82] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [83] "  A: 65.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [84] "  C: 28.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [85] "  G: 3.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [86] "  T: 3.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [87] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [88] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [89] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [90] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [91] "5\t4\t6.8\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [92] "6\t2\t1.7\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [93] "7\t2\t0.4\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [94] "8\t3\t0.1\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [95] "9\t4\t0.0\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [96] "11\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [97] "12\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [98] "13\t9\t0.0\t1\t9"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [99] "14\t9\t0.0\t1\t8 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [100] "15\t8\t0.0\t1\t5 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [101] "16\t8\t0.0\t1\t6 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [102] "17\t7\t0.0\t1\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [103] "18\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [104] "20\t7\t0.0\t2\t5 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [105] "21\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [106] "22\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [107] "23\t3\t0.0\t2\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [108] "24\t4\t0.0\t2\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [109] "25\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [110] "26\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [111] "27\t5\t0.0\t2\t3 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [112] "28\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [113] "29\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [114] "30\t4\t0.0\t3\t2 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [115] "31\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [116] "33\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [117] "34\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [118] "35\t4\t0.0\t3\t2 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [119] "36\t2\t0.0\t3\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [120] "37\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [121] "38\t4\t0.0\t3\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [122] "39\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [123] "40\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [124] "41\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [125] "42\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [126] "43\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [127] "44\t2\t0.0\t4\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [128] "45\t5\t0.0\t4\t3 1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [129] "46\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [130] "47\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [131] "48\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [132] "49\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [133] "51\t4\t0.0\t4\t2 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [134] "52\t5\t0.0\t4\t2 2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [135] "53\t4\t0.0\t4\t1 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [136] "54\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [137] "55\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [138] "56\t3\t0.0\t4\t1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [139] "57\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [140] "58\t6\t0.0\t4\t5 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [141] "59\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [142] "60\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [143] "61\t1\t0.0\t4\t0 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [144] "63\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [145] "64\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [146] "65\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [147] "66\t5\t0.0\t4\t3 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [148] "67\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [149] "69\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [150] "70\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [151] "71\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [152] "72\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [153] "73\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [154] "74\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [155] "75\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [156] "76\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [157] "77\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [158] "78\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [159] "79\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [160] "81\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [161] "82\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [162] "84\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [163] "85\t7\t0.0\t4\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [164] "86\t8\t0.0\t4\t6 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [165] "87\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [166] "88\t3\t0.0\t4\t1 0 0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [167] "89\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [168] "91\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [169] "92\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [170] "93\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [171] "94\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [172] "95\t3\t0.0\t4\t2 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [173] "96\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [174] "97\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [175] "98\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [176] "99\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [177] "101\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [178] "104\t7\t0.0\t4\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [179] "105\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [180] "106\t4\t0.0\t4\t3 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [181] "107\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [182] "110\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [183] "111\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [184] "112\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [185] "114\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [186] "116\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [187] "117\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [188] "118\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [189] "119\t2\t0.0\t4\t1 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [190] "120\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [191] "121\t5\t0.0\t4\t3 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [192] "122\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [193] "123\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [194] "125\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [195] "128\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [196] "129\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [197] "133\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [198] "135\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [199] "136\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [200] "138\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [201] "139\t3\t0.0\t4\t2 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [202] "140\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [203] "141\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [204] "142\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [205] "146\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [206] "147\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [207] "148\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [208] "149\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [209] "158\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [210] "160\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [211] "172\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## 
## [[30]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/1882_S30_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/1882_S30_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/1882_S30_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/1882_S30_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##   [4] "Finished in 0.74 s (125 us/read; 0.48 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [8] "Total read pairs processed:              5,922"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [9] "  Read 1 with adapter:                      44 (0.7%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [10] "  Read 2 with adapter:                     282 (4.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [11] "Pairs written (passing filters):         5,921 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [13] "Total basepairs processed:     2,487,240 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [14] "  Read 1:     1,160,712 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [15] "  Read 2:     1,326,528 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [16] "Total written (filtered):      2,468,571 bp (99.2%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [17] "  Read 1:     1,159,473 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [18] "  Read 2:     1,309,098 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 28 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [29] "6\t1\t1.4\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [30] "14\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [31] "17\t19\t0.0\t1\t18 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [32] "21\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [33] "30\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [34] "31\t4\t0.0\t3\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [35] "60\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [36] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [38] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [39] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [40] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 16 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [41] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [42] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [43] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [44] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [45] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [46] "  A: 25.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [47] "  C: 43.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [48] "  G: 25.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [49] "  T: 6.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [50] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [51] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [52] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [53] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [54] "6\t1\t1.4\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [55] "7\t1\t0.4\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [56] "13\t7\t0.0\t1\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [57] "29\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [58] "38\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [59] "39\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [60] "75\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [61] "78\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [62] "87\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [63] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [64] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [65] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [66] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [67] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [68] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [69] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [70] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [71] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [72] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [73] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [74] "25\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [75] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [76] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [77] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [78] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [79] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 281 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [80] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [81] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [82] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [83] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [84] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [85] "  A: 60.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [86] "  C: 29.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [87] "  G: 5.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [88] "  T: 3.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [89] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [90] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [91] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [92] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [93] "5\t6\t5.8\t0\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [94] "6\t5\t1.4\t0\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [95] "8\t3\t0.1\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [96] "9\t1\t0.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [97] "10\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [98] "12\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [99] "13\t8\t0.0\t1\t7 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [100] "14\t6\t0.0\t1\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [101] "15\t5\t0.0\t1\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [102] "16\t4\t0.0\t1\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [103] "17\t4\t0.0\t1\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [104] "18\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [105] "19\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [106] "20\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [107] "21\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [108] "22\t4\t0.0\t2\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [109] "23\t9\t0.0\t2\t8 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [110] "24\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [111] "25\t3\t0.0\t2\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [112] "26\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [113] "27\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [114] "28\t4\t0.0\t2\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [115] "31\t3\t0.0\t3\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [116] "32\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [117] "33\t5\t0.0\t3\t3 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [118] "34\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [119] "35\t4\t0.0\t3\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [120] "36\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [121] "37\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [122] "38\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [123] "39\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [124] "40\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [125] "41\t10\t0.0\t4\t9 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [126] "42\t5\t0.0\t4\t4 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [127] "43\t3\t0.0\t4\t2 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [128] "44\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [129] "45\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [130] "46\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [131] "47\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [132] "48\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [133] "50\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [134] "51\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [135] "52\t2\t0.0\t4\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [136] "53\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [137] "54\t5\t0.0\t4\t4 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [138] "55\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [139] "56\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [140] "57\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [141] "58\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [142] "59\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [143] "60\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [144] "61\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [145] "62\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [146] "63\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [147] "64\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [148] "65\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [149] "66\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [150] "67\t9\t0.0\t4\t9"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [151] "69\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [152] "70\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [153] "71\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [154] "72\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [155] "73\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [156] "74\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [157] "75\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [158] "77\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [159] "78\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [160] "79\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [161] "80\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [162] "81\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [163] "82\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [164] "83\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [165] "84\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [166] "85\t7\t0.0\t4\t6 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [167] "86\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [168] "87\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [169] "88\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [170] "89\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [171] "92\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [172] "93\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [173] "94\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [174] "95\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [175] "96\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [176] "98\t3\t0.0\t4\t2 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [177] "103\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [178] "104\t6\t0.0\t4\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [179] "105\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [180] "106\t5\t0.0\t4\t4 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [181] "108\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [182] "110\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [183] "112\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [184] "113\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [185] "114\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [186] "115\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [187] "118\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [188] "121\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [189] "126\t1\t0.0\t4\t0 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [190] "127\t2\t0.0\t4\t1 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [191] "131\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [192] "134\t1\t0.0\t4\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [193] "135\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [194] "138\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [195] "139\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [196] "140\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [197] "141\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [198] "143\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [199] "146\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [200] "147\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [201] "148\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [202] "151\t1\t0.0\t4\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [203] "152\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [204] "161\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [205] "173\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [206] "190\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## 
## [[31]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/1883_S31_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/1883_S31_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/1883_S31_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/1883_S31_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##   [4] "Finished in 1.28 s (131 us/read; 0.46 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [8] "Total read pairs processed:              9,767"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [9] "  Read 1 with adapter:                      81 (0.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [10] "  Read 2 with adapter:                     327 (3.3%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [11] "Pairs written (passing filters):         9,767 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [13] "Total basepairs processed:     4,102,140 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [14] "  Read 1:     1,914,332 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [15] "  Read 2:     2,187,808 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [16] "Total written (filtered):      4,079,675 bp (99.5%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [17] "  Read 1:     1,912,267 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [18] "  Read 2:     2,167,408 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 75 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [29] "6\t1\t2.4\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [30] "12\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [31] "15\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [32] "16\t7\t0.0\t1\t4 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [33] "17\t35\t0.0\t1\t31 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [34] "26\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [35] "27\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [36] "28\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [37] "29\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [38] "30\t2\t0.0\t3\t0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [39] "31\t19\t0.0\t3\t13 6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [40] "48\t3\t0.0\t4\t0 0 2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [41] "61\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [42] "62\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [43] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [44] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [45] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [46] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [47] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 6 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [48] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [49] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [50] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [51] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [52] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [53] "  A: 16.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [54] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [55] "  G: 83.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [56] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [57] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [58] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [59] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [60] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [61] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [62] "33\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [63] "34\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [64] "38\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [65] "82\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [66] "95\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [67] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [68] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [69] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [70] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [71] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 4 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [72] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [73] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [74] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [75] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [76] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [77] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [78] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [79] "20\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [80] "29\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [81] "73\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [82] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [83] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [84] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [85] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [86] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 323 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [87] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [88] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [89] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [90] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [91] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [92] "  A: 61.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [93] "  C: 27.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [94] "  G: 5.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [95] "  T: 5.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [96] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [97] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [98] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [99] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [100] "5\t10\t9.5\t0\t10"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [101] "7\t2\t0.6\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [102] "8\t10\t0.1\t0\t10"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [103] "9\t3\t0.0\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [104] "10\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [105] "11\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [106] "12\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [107] "13\t5\t0.0\t1\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [108] "14\t5\t0.0\t1\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [109] "15\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [110] "16\t4\t0.0\t1\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [111] "17\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [112] "18\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [113] "19\t6\t0.0\t1\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [114] "20\t5\t0.0\t2\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [115] "21\t11\t0.0\t2\t6 3 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [116] "22\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [117] "23\t4\t0.0\t2\t2 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [118] "24\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [119] "25\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [120] "26\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [121] "27\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [122] "30\t3\t0.0\t3\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [123] "31\t3\t0.0\t3\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [124] "32\t3\t0.0\t3\t2 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [125] "33\t4\t0.0\t3\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [126] "34\t3\t0.0\t3\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [127] "35\t4\t0.0\t3\t2 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [128] "37\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [129] "38\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [130] "39\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [131] "41\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [132] "42\t5\t0.0\t4\t3 1 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [133] "43\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [134] "45\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [135] "46\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [136] "47\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [137] "48\t5\t0.0\t4\t4 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [138] "49\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [139] "50\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [140] "51\t6\t0.0\t4\t4 1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [141] "52\t6\t0.0\t4\t4 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [142] "54\t5\t0.0\t4\t4 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [143] "55\t3\t0.0\t4\t1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [144] "57\t2\t0.0\t4\t1 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [145] "58\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [146] "60\t6\t0.0\t4\t2 1 0 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [147] "61\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [148] "62\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [149] "63\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [150] "64\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [151] "65\t4\t0.0\t4\t1 1 0 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [152] "66\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [153] "67\t3\t0.0\t4\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [154] "69\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [155] "71\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [156] "72\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [157] "73\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [158] "74\t4\t0.0\t4\t3 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [159] "75\t2\t0.0\t4\t0 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [160] "76\t2\t0.0\t4\t1 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [161] "77\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [162] "79\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [163] "80\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [164] "81\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [165] "82\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [166] "84\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [167] "85\t5\t0.0\t4\t4 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [168] "86\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [169] "87\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [170] "88\t7\t0.0\t4\t5 0 0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [171] "89\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [172] "92\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [173] "93\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [174] "94\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [175] "95\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [176] "96\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [177] "97\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [178] "98\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [179] "99\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [180] "101\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [181] "102\t2\t0.0\t4\t1 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [182] "103\t4\t0.0\t4\t1 2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [183] "104\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [184] "105\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [185] "107\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [186] "108\t1\t0.0\t4\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [187] "109\t1\t0.0\t4\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [188] "110\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [189] "111\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [190] "112\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [191] "113\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [192] "114\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [193] "115\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [194] "116\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [195] "117\t2\t0.0\t4\t0 1 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [196] "118\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [197] "120\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [198] "121\t3\t0.0\t4\t2 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [199] "125\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [200] "126\t1\t0.0\t4\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [201] "127\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [202] "129\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [203] "134\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [204] "136\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [205] "139\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [206] "140\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [207] "141\t2\t0.0\t4\t1 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [208] "144\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [209] "146\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [210] "147\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [211] "148\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [212] "150\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [213] "152\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [214] "154\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [215] "161\t1\t0.0\t4\t0 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [216] "167\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [217] "171\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [218] "174\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## 
## [[32]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/1884_S32_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/1884_S32_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/1884_S32_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/1884_S32_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##   [4] "Finished in 2.10 s (120 us/read; 0.50 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [8] "Total read pairs processed:             17,599"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [9] "  Read 1 with adapter:                      23 (0.1%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [10] "  Read 2 with adapter:                     147 (0.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [11] "Pairs written (passing filters):        17,599 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [13] "Total basepairs processed:     7,391,580 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [14] "  Read 1:     3,449,404 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [15] "  Read 2:     3,942,176 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [16] "Total written (filtered):      7,382,734 bp (99.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [17] "  Read 1:     3,448,755 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [18] "  Read 2:     3,933,979 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 22 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [29] "8\t1\t0.3\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [30] "17\t10\t0.0\t1\t10"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [31] "21\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [32] "30\t4\t0.0\t3\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [33] "31\t5\t0.0\t3\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [34] "93\t1\t0.0\t4\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [36] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [37] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [39] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [40] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [41] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [42] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [43] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [44] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [45] "  A: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [46] "  C: 100.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [47] "  G: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [48] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [49] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [50] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [51] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [52] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [53] "82\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [54] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [55] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [56] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [57] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [58] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [59] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [60] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [61] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [62] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [63] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [64] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [65] "6\t1\t4.3\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [66] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [67] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [68] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [69] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [70] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 147 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [71] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [72] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [73] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [74] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [75] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [76] "  A: 61.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [77] "  C: 24.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [78] "  G: 5.4%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [79] "  T: 8.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [80] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [81] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [82] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [83] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [84] "5\t12\t17.2\t0\t12"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [85] "6\t2\t4.3\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [86] "7\t1\t1.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [87] "8\t5\t0.3\t0\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [88] "9\t3\t0.1\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [89] "11\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [90] "13\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [91] "14\t2\t0.0\t1\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [92] "15\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [93] "16\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [94] "18\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [95] "19\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [96] "20\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [97] "21\t7\t0.0\t2\t4 2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [98] "22\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [99] "23\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [100] "26\t2\t0.0\t2\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [101] "28\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [102] "29\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [103] "31\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [104] "33\t2\t0.0\t3\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [105] "34\t3\t0.0\t3\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [106] "35\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [107] "37\t1\t0.0\t3\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [108] "38\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [109] "39\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [110] "42\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [111] "44\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [112] "48\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [113] "51\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [114] "52\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [115] "53\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [116] "55\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [117] "58\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [118] "59\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [119] "60\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [120] "61\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [121] "62\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [122] "63\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [123] "64\t7\t0.0\t4\t5 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [124] "65\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [125] "66\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [126] "67\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [127] "69\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [128] "72\t2\t0.0\t4\t1 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [129] "77\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [130] "78\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [131] "82\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [132] "83\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [133] "84\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [134] "86\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [135] "88\t2\t0.0\t4\t1 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [136] "89\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [137] "90\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [138] "91\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [139] "92\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [140] "94\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [141] "96\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [142] "97\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [143] "98\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [144] "99\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [145] "100\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [146] "102\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [147] "104\t3\t0.0\t4\t1 0 0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [148] "105\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [149] "106\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [150] "107\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [151] "108\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [152] "110\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [153] "112\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [154] "113\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [155] "115\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [156] "119\t2\t0.0\t4\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [157] "121\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [158] "127\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [159] "129\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [160] "138\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## 
## [[33]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/1885_S33_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/1885_S33_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/1885_S33_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/1885_S33_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##   [4] "Finished in 1.73 s (120 us/read; 0.50 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [8] "Total read pairs processed:             14,416"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [9] "  Read 1 with adapter:                       9 (0.1%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [10] "  Read 2 with adapter:                     111 (0.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [11] "Pairs written (passing filters):        14,416 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [13] "Total basepairs processed:     6,054,720 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [14] "  Read 1:     2,825,536 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [15] "  Read 2:     3,229,184 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [16] "Total written (filtered):      6,049,090 bp (99.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [17] "  Read 1:     2,825,183 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [18] "  Read 2:     3,223,907 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 3 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [29] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [30] "31\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [31] "79\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [32] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [34] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [36] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 6 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [38] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [39] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [40] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [41] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [42] "  A: 16.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [43] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [44] "  G: 83.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [45] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [46] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [47] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [48] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [49] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [50] "8\t1\t0.2\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [51] "18\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [52] "32\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [53] "38\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [54] "92\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [55] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [56] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [57] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [58] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [59] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 2 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [60] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [61] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [62] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [63] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [64] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [65] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [66] "6\t1\t3.5\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [67] "26\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [68] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [69] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [70] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [71] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [72] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 111 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [73] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [74] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [75] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [76] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [77] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [78] "  A: 59.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [79] "  C: 31.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [80] "  G: 5.4%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [81] "  T: 3.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [82] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [83] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [84] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [85] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [86] "5\t13\t14.1\t0\t13"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [87] "6\t2\t3.5\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [88] "8\t7\t0.2\t0\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [89] "9\t2\t0.1\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [90] "10\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [91] "11\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [92] "14\t2\t0.0\t1\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [93] "15\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [94] "16\t2\t0.0\t1\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [95] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [96] "20\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [97] "23\t2\t0.0\t2\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [98] "24\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [99] "25\t1\t0.0\t2\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [100] "26\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [101] "27\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [102] "28\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [103] "29\t1\t0.0\t2\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [104] "31\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [105] "33\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [106] "34\t3\t0.0\t3\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [107] "35\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [108] "41\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [109] "42\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [110] "43\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [111] "44\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [112] "45\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [113] "48\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [114] "50\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [115] "51\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [116] "52\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [117] "54\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [118] "55\t2\t0.0\t4\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [119] "56\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [120] "57\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [121] "60\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [122] "63\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [123] "64\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [124] "66\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [125] "67\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [126] "71\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [127] "72\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [128] "74\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [129] "76\t2\t0.0\t4\t1 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [130] "77\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [131] "78\t2\t0.0\t4\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [132] "79\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [133] "81\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [134] "84\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [135] "85\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [136] "86\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [137] "88\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [138] "91\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [139] "92\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [140] "98\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [141] "104\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [142] "105\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [143] "107\t1\t0.0\t4\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [144] "108\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [145] "116\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [146] "119\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [147] "120\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [148] "121\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [149] "123\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [150] "126\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [151] "144\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## 
## [[34]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/1886_S34_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/1886_S34_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/1886_S34_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/1886_S34_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##   [4] "Finished in 2.32 s (121 us/read; 0.49 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [8] "Total read pairs processed:             19,137"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [9] "  Read 1 with adapter:                       3 (0.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [10] "  Read 2 with adapter:                     117 (0.6%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [11] "Pairs written (passing filters):        19,137 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [13] "Total basepairs processed:     8,037,540 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [14] "  Read 1:     3,750,852 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [15] "  Read 2:     4,286,688 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [16] "Total written (filtered):      8,031,739 bp (99.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [17] "  Read 1:     3,750,799 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [18] "  Read 2:     4,280,940 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 3 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [29] "5\t1\t18.7\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [30] "14\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [31] "34\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [32] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [34] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [36] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [38] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [39] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [40] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 3 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [41] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [42] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [43] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [44] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [45] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [46] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [47] "5\t1\t18.7\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [48] "12\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [49] "21\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [50] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [51] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [52] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [53] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [54] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 115 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [55] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [56] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [57] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [58] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [59] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [60] "  A: 59.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [61] "  C: 29.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [62] "  G: 8.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [63] "  T: 2.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [64] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [65] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [66] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [67] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [68] "5\t14\t18.7\t0\t14"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [69] "6\t2\t4.7\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [70] "8\t4\t0.3\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [71] "9\t2\t0.1\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [72] "11\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [73] "14\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [74] "15\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [75] "16\t3\t0.0\t1\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [76] "17\t6\t0.0\t1\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [77] "19\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [78] "20\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [79] "21\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [80] "22\t3\t0.0\t2\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [81] "23\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [82] "24\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [83] "26\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [84] "27\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [85] "28\t2\t0.0\t2\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [86] "29\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [87] "31\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [88] "32\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [89] "35\t4\t0.0\t3\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [90] "37\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [91] "40\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [92] "41\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [93] "42\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [94] "43\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [95] "45\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [96] "51\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [97] "52\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [98] "53\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [99] "54\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [100] "56\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [101] "57\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [102] "58\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [103] "61\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [104] "62\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [105] "63\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [106] "66\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [107] "73\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [108] "74\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [109] "75\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [110] "79\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [111] "82\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [112] "84\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [113] "85\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [114] "90\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [115] "93\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [116] "94\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [117] "97\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [118] "100\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [119] "101\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [120] "103\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [121] "104\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [122] "105\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [123] "107\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [124] "108\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [125] "109\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [126] "113\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [127] "120\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [128] "125\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [129] "139\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [130] "149\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [131] "154\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [132] "156\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [133] "165\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## 
## [[35]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/1887_S35_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/1887_S35_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/1887_S35_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/1887_S35_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##   [4] "Finished in 2.08 s (121 us/read; 0.50 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [8] "Total read pairs processed:             17,213"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [9] "  Read 1 with adapter:                      10 (0.1%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [10] "  Read 2 with adapter:                     147 (0.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [11] "Pairs written (passing filters):        17,213 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [13] "Total basepairs processed:     7,229,460 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [14] "  Read 1:     3,373,748 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [15] "  Read 2:     3,855,712 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [16] "Total written (filtered):      7,219,994 bp (99.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [17] "  Read 1:     3,373,639 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [18] "  Read 2:     3,846,355 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 4 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [29] "5\t2\t16.8\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [30] "24\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [31] "25\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [32] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [34] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [36] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 6 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [38] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [39] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [40] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [41] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [42] "  A: 100.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [43] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [44] "  G: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [45] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [46] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [47] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [48] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [49] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [50] "6\t1\t4.2\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [51] "7\t1\t1.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [52] "9\t3\t0.1\t0\t1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [53] "10\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [54] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [55] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [56] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [57] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [58] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [59] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [60] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [61] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [62] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [63] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [64] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [65] "6\t1\t4.2\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [66] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [67] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [68] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [69] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [70] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 146 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [71] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [72] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [73] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [74] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [75] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [76] "  A: 67.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [77] "  C: 24.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [78] "  G: 4.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [79] "  T: 4.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [80] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [81] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [82] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [83] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [84] "5\t8\t16.8\t0\t8"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [85] "6\t1\t4.2\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [86] "7\t1\t1.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [87] "9\t2\t0.1\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [88] "10\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [89] "14\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [90] "16\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [91] "17\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [92] "19\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [93] "20\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [94] "21\t4\t0.0\t2\t0 3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [95] "22\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [96] "23\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [97] "25\t3\t0.0\t2\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [98] "26\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [99] "29\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [100] "30\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [101] "33\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [102] "34\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [103] "35\t3\t0.0\t3\t1 1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [104] "36\t2\t0.0\t3\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [105] "41\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [106] "42\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [107] "43\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [108] "44\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [109] "45\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [110] "46\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [111] "48\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [112] "50\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [113] "51\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [114] "54\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [115] "55\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [116] "57\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [117] "58\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [118] "62\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [119] "65\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [120] "66\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [121] "69\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [122] "70\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [123] "71\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [124] "72\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [125] "75\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [126] "76\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [127] "77\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [128] "79\t5\t0.0\t4\t3 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [129] "80\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [130] "81\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [131] "82\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [132] "83\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [133] "84\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [134] "85\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [135] "86\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [136] "89\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [137] "91\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [138] "92\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [139] "94\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [140] "96\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [141] "98\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [142] "99\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [143] "101\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [144] "102\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [145] "103\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [146] "104\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [147] "105\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [148] "108\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [149] "110\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [150] "111\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [151] "112\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [152] "113\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [153] "116\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [154] "119\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [155] "122\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [156] "127\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [157] "128\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [158] "131\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [159] "134\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [160] "137\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [161] "141\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [162] "142\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [163] "146\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [164] "148\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [165] "159\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## 
## [[36]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/188RNA1_S36_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/188RNA1_S36_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/188RNA1_S36_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/188RNA1_S36_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##   [4] "Finished in 0.34 s (124 us/read; 0.48 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [8] "Total read pairs processed:              2,776"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##   [9] "  Read 1 with adapter:                      12 (0.4%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [10] "  Read 2 with adapter:                     210 (7.6%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [11] "Pairs written (passing filters):         2,776 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [13] "Total basepairs processed:     1,165,920 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [14] "  Read 1:       544,096 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [15] "  Read 2:       621,824 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [16] "Total written (filtered):      1,149,566 bp (98.6%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [17] "  Read 1:       543,704 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [18] "  Read 2:       605,862 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 6 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [29] "16\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [30] "17\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [31] "30\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [32] "31\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [33] "49\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [34] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [36] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [38] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 6 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [39] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [40] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [41] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [42] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [43] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [44] "  A: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [45] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [46] "  G: 83.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [47] "  T: 16.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [48] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [49] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [50] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [51] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [52] "6\t1\t0.7\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [53] "28\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [54] "36\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [55] "90\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [56] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [57] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [58] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [59] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [60] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [61] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [62] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [63] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [64] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 210 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [65] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [66] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [67] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [68] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [69] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [70] "  A: 55.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [71] "  C: 35.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [72] "  G: 6.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [73] "  T: 2.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [74] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [75] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [76] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [77] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [78] "6\t1\t0.7\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [79] "7\t2\t0.2\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [80] "8\t4\t0.0\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [81] "9\t2\t0.0\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [82] "12\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [83] "13\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [84] "14\t2\t0.0\t1\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [85] "16\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [86] "20\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [87] "21\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [88] "22\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [89] "24\t1\t0.0\t2\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [90] "25\t4\t0.0\t2\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [91] "26\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [92] "27\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [93] "28\t4\t0.0\t2\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [94] "29\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [95] "32\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [96] "33\t1\t0.0\t3\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [97] "34\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [98] "35\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [99] "40\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [100] "41\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [101] "42\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [102] "43\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [103] "45\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [104] "46\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [105] "48\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [106] "50\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [107] "51\t2\t0.0\t4\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [108] "52\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [109] "53\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [110] "55\t2\t0.0\t4\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [111] "56\t5\t0.0\t4\t2 1 0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [112] "57\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [113] "58\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [114] "59\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [115] "60\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [116] "61\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [117] "62\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [118] "64\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [119] "65\t4\t0.0\t4\t3 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [120] "66\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [121] "67\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [122] "69\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [123] "70\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [124] "71\t2\t0.0\t4\t1 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [125] "72\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [126] "73\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [127] "74\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [128] "75\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [129] "77\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [130] "79\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [131] "82\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [132] "83\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [133] "84\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [134] "85\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [135] "86\t8\t0.0\t4\t8"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [136] "87\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [137] "88\t4\t0.0\t4\t3 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [138] "89\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [139] "92\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [140] "94\t9\t0.0\t4\t8 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [141] "95\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [142] "96\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [143] "97\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [144] "100\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [145] "101\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [146] "103\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [147] "105\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [148] "106\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [149] "108\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [150] "109\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [151] "110\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [152] "111\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [153] "112\t3\t0.0\t4\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [154] "113\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [155] "115\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [156] "117\t4\t0.0\t4\t3 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [157] "118\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [158] "119\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [159] "120\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [160] "121\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [161] "122\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [162] "125\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [163] "127\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [164] "129\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [165] "130\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [166] "132\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [167] "133\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [168] "138\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [169] "140\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [170] "144\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [171] "145\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [172] "146\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [173] "148\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [174] "150\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [175] "151\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [176] "160\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [177] "162\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## 
## [[37]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/188RNA2_S37_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/188RNA2_S37_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/188RNA2_S37_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/188RNA2_S37_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##   [4] "Finished in 0.38 s (120 us/read; 0.50 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [8] "Total read pairs processed:              3,178"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##   [9] "  Read 1 with adapter:                      12 (0.4%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [10] "  Read 2 with adapter:                     146 (4.6%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [11] "Pairs written (passing filters):         3,176 (99.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [13] "Total basepairs processed:     1,334,760 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [14] "  Read 1:       622,888 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [15] "  Read 2:       711,872 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [16] "Total written (filtered):      1,323,707 bp (99.2%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [17] "  Read 1:       622,114 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [18] "  Read 2:       701,593 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 2 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [29] "11\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [30] "15\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [32] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [33] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [34] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [35] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 10 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [36] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [37] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [38] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [39] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [40] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [41] "  A: 60.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [42] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [43] "  G: 40.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [44] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [45] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [46] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [47] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [48] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [49] "5\t1\t3.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [50] "15\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [51] "20\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [52] "36\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [53] "37\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [54] "48\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [55] "59\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [56] "64\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [57] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [58] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [59] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [60] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [61] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [62] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [63] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [64] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [65] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 146 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [66] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [67] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [68] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [69] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [70] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [71] "  A: 54.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [72] "  C: 39.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [73] "  G: 4.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [74] "  T: 2.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [75] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [76] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [77] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [78] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [79] "5\t1\t3.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [80] "8\t2\t0.0\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [81] "9\t1\t0.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [82] "10\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [83] "12\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [84] "14\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [85] "15\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [86] "16\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [87] "20\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [88] "21\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [89] "22\t3\t0.0\t2\t1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [90] "23\t5\t0.0\t2\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [91] "24\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [92] "25\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [93] "27\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [94] "28\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [95] "30\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [96] "33\t3\t0.0\t3\t1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [97] "34\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [98] "36\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [99] "37\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [100] "39\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [101] "41\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [102] "42\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [103] "43\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [104] "44\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [105] "45\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [106] "48\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [107] "49\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [108] "50\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [109] "52\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [110] "55\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [111] "56\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [112] "57\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [113] "60\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [114] "61\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [115] "63\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [116] "64\t7\t0.0\t4\t6 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [117] "65\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [118] "66\t3\t0.0\t4\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [119] "67\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [120] "68\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [121] "69\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [122] "72\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [123] "74\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [124] "75\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [125] "76\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [126] "77\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [127] "78\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [128] "80\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [129] "81\t3\t0.0\t4\t1 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [130] "82\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [131] "83\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [132] "84\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [133] "86\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [134] "87\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [135] "88\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [136] "89\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [137] "91\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [138] "92\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [139] "93\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [140] "94\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [141] "95\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [142] "96\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [143] "98\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [144] "104\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [145] "105\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [146] "106\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [147] "108\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [148] "110\t1\t0.0\t4\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [149] "111\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [150] "112\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [151] "113\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [152] "116\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [153] "119\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [154] "120\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [155] "127\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [156] "130\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [157] "134\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [158] "140\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [159] "141\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [160] "146\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [161] "147\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [162] "148\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [163] "162\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [164] "170\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [165] "177\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [166] "186\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## 
## [[38]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/188RNA3_S38_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/188RNA3_S38_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/188RNA3_S38_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/188RNA3_S38_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##   [4] "Finished in 0.49 s (126 us/read; 0.47 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [8] "Total read pairs processed:              3,881"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##   [9] "  Read 1 with adapter:                     102 (2.6%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [10] "  Read 2 with adapter:                     265 (6.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [11] "Pairs written (passing filters):         3,880 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [13] "Total basepairs processed:     1,630,020 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [14] "  Read 1:       760,676 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [15] "  Read 2:       869,344 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [16] "Total written (filtered):      1,608,990 bp (98.7%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [17] "  Read 1:       757,923 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [18] "  Read 2:       851,067 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 89 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [29] "7\t1\t0.2\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [30] "8\t2\t0.1\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [31] "13\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [32] "16\t2\t0.0\t1\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [33] "17\t57\t0.0\t1\t56 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [34] "25\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [35] "26\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [36] "27\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [37] "28\t6\t0.0\t2\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [38] "31\t14\t0.0\t3\t10 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [39] "32\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [40] "33\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [41] "48\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [42] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [43] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [44] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [45] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [46] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 13 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [47] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [48] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [49] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [50] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [51] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [52] "  A: 30.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [53] "  C: 7.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [54] "  G: 46.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [55] "  T: 15.4%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [56] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [57] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [58] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [59] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [60] "5\t1\t3.8\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [61] "17\t2\t0.0\t1\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [62] "18\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [63] "48\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [64] "59\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [65] "60\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [66] "63\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [67] "70\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [68] "82\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [69] "83\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [70] "100\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [71] "105\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [72] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [73] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [74] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [75] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [76] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 4 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [77] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [78] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [79] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [80] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [81] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [82] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [83] "17\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [84] "27\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [85] "30\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [86] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [87] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [88] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [89] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [90] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 261 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [91] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [92] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [93] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [94] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [95] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [96] "  A: 64.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [97] "  C: 26.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [98] "  G: 1.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [99] "  T: 6.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [100] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [101] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [102] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [103] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [104] "5\t4\t3.8\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [105] "6\t1\t0.9\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [106] "7\t1\t0.2\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [107] "8\t2\t0.1\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [108] "9\t1\t0.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [109] "11\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [110] "12\t2\t0.0\t1\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [111] "13\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [112] "14\t4\t0.0\t1\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [113] "15\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [114] "16\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [115] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [116] "18\t3\t0.0\t1\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [117] "19\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [118] "21\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [119] "22\t2\t0.0\t2\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [120] "23\t4\t0.0\t2\t2 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [121] "25\t6\t0.0\t2\t3 1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [122] "26\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [123] "27\t4\t0.0\t2\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [124] "30\t4\t0.0\t3\t2 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [125] "31\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [126] "32\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [127] "33\t4\t0.0\t3\t2 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [128] "34\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [129] "35\t4\t0.0\t3\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [130] "36\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [131] "38\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [132] "39\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [133] "41\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [134] "42\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [135] "44\t1\t0.0\t4\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [136] "45\t6\t0.0\t4\t4 1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [137] "46\t6\t0.0\t4\t4 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [138] "47\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [139] "48\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [140] "50\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [141] "51\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [142] "52\t6\t0.0\t4\t4 1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [143] "53\t4\t0.0\t4\t3 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [144] "54\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [145] "56\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [146] "57\t3\t0.0\t4\t2 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [147] "58\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [148] "59\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [149] "60\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [150] "64\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [151] "65\t1\t0.0\t4\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [152] "66\t1\t0.0\t4\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [153] "67\t6\t0.0\t4\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [154] "68\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [155] "69\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [156] "70\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [157] "72\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [158] "74\t3\t0.0\t4\t2 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [159] "75\t6\t0.0\t4\t4 1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [160] "76\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [161] "77\t3\t0.0\t4\t2 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [162] "78\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [163] "79\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [164] "80\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [165] "81\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [166] "82\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [167] "83\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [168] "84\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [169] "85\t4\t0.0\t4\t3 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [170] "86\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [171] "87\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [172] "88\t3\t0.0\t4\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [173] "90\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [174] "91\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [175] "92\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [176] "93\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [177] "94\t6\t0.0\t4\t4 0 0 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [178] "95\t2\t0.0\t4\t0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [179] "96\t3\t0.0\t4\t1 1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [180] "98\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [181] "99\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [182] "100\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [183] "101\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [184] "102\t1\t0.0\t4\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [185] "104\t3\t0.0\t4\t1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [186] "105\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [187] "107\t2\t0.0\t4\t1 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [188] "108\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [189] "110\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [190] "111\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [191] "112\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [192] "113\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [193] "115\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [194] "116\t3\t0.0\t4\t1 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [195] "117\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [196] "119\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [197] "121\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [198] "122\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [199] "128\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [200] "129\t1\t0.0\t4\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [201] "130\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [202] "132\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [203] "133\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [204] "134\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [205] "135\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [206] "139\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [207] "141\t1\t0.0\t4\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [208] "142\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [209] "143\t1\t0.0\t4\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [210] "144\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [211] "146\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [212] "147\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [213] "158\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [214] "162\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [215] "167\t1\t0.0\t4\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [216] "172\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [217] "186\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## 
## [[39]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/188RNA4_S39_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/188RNA4_S39_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/188RNA4_S39_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/188RNA4_S39_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##   [4] "Finished in 0.52 s (125 us/read; 0.48 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [8] "Total read pairs processed:              4,143"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##   [9] "  Read 1 with adapter:                      68 (1.6%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [10] "  Read 2 with adapter:                     181 (4.4%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [11] "Pairs written (passing filters):         4,142 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [13] "Total basepairs processed:     1,740,060 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [14] "  Read 1:       812,028 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [15] "  Read 2:       928,032 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [16] "Total written (filtered):      1,722,841 bp (99.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [17] "  Read 1:       809,080 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [18] "  Read 2:       913,761 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 47 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [29] "8\t1\t0.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [30] "15\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [31] "16\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [32] "17\t27\t0.0\t1\t26 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [33] "27\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [34] "30\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [35] "31\t10\t0.0\t3\t9 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [36] "32\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [37] "61\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [38] "62\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [39] "63\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [40] "79\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [41] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [42] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [43] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [44] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [45] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 21 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [46] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [47] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [48] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [49] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [50] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [51] "  A: 57.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [52] "  C: 4.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [53] "  G: 38.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [54] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [55] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [56] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [57] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [58] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [59] "16\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [60] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [61] "18\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [62] "49\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [63] "59\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [64] "66\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [65] "71\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [66] "74\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [67] "82\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [68] "90\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [69] "101\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [70] "102\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [71] "118\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [72] "122\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [73] "125\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [74] "143\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [75] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [76] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [77] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [78] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [79] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 3 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [80] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [81] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [82] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [83] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [84] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [85] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [86] "18\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [87] "30\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [88] "48\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [89] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [90] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [91] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [92] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [93] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 178 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [94] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [95] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [96] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [97] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [98] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [99] "  A: 74.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [100] "  C: 19.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [101] "  G: 3.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [102] "  T: 2.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [103] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [104] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [105] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [106] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [107] "5\t2\t4.0\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [108] "6\t1\t1.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [109] "16\t4\t0.0\t1\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [110] "17\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [111] "20\t1\t0.0\t2\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [112] "22\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [113] "23\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [114] "24\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [115] "25\t9\t0.0\t2\t8 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [116] "27\t1\t0.0\t2\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [117] "31\t3\t0.0\t3\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [118] "33\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [119] "35\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [120] "37\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [121] "39\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [122] "41\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [123] "42\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [124] "43\t3\t0.0\t4\t2 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [125] "44\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [126] "45\t5\t0.0\t4\t3 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [127] "46\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [128] "47\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [129] "49\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [130] "50\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [131] "51\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [132] "52\t4\t0.0\t4\t1 1 0 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [133] "53\t1\t0.0\t4\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [134] "54\t1\t0.0\t4\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [135] "57\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [136] "58\t2\t0.0\t4\t1 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [137] "59\t3\t0.0\t4\t2 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [138] "63\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [139] "65\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [140] "67\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [141] "69\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [142] "70\t1\t0.0\t4\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [143] "72\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [144] "73\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [145] "74\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [146] "75\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [147] "76\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [148] "77\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [149] "78\t4\t0.0\t4\t3 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [150] "79\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [151] "82\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [152] "84\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [153] "86\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [154] "87\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [155] "88\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [156] "89\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [157] "90\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [158] "91\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [159] "92\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [160] "93\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [161] "94\t9\t0.0\t4\t8 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [162] "95\t1\t0.0\t4\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [163] "96\t1\t0.0\t4\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [164] "97\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [165] "99\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [166] "100\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [167] "101\t2\t0.0\t4\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [168] "102\t3\t0.0\t4\t2 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [169] "103\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [170] "104\t4\t0.0\t4\t3 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [171] "105\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [172] "106\t1\t0.0\t4\t0 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [173] "107\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [174] "109\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [175] "111\t1\t0.0\t4\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [176] "112\t3\t0.0\t4\t2 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [177] "113\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [178] "115\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [179] "118\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [180] "120\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [181] "121\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [182] "125\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [183] "126\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [184] "127\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [185] "129\t3\t0.0\t4\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [186] "130\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [187] "138\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [188] "140\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [189] "143\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [190] "144\t1\t0.0\t4\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [191] "146\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [192] "147\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [193] "150\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [194] "151\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [195] "153\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [196] "159\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [197] "160\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [198] "171\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [199] "173\t1\t0.0\t4\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [200] "175\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## 
## [[40]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/188RNA5_S40_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/188RNA5_S40_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/188RNA5_S40_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/188RNA5_S40_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##   [4] "Finished in 0.62 s (124 us/read; 0.48 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [8] "Total read pairs processed:              5,004"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##   [9] "  Read 1 with adapter:                      72 (1.4%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [10] "  Read 2 with adapter:                     196 (3.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [11] "Pairs written (passing filters):         5,003 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [13] "Total basepairs processed:     2,101,680 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [14] "  Read 1:       980,784 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [15] "  Read 2:     1,120,896 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [16] "Total written (filtered):      2,081,169 bp (99.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [17] "  Read 1:       975,805 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [18] "  Read 2:     1,105,364 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 2 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [29] "5\t1\t4.9\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [30] "30\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [32] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [33] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [34] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [35] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 70 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [36] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [37] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [38] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [39] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [40] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [41] "  A: 20.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [42] "  C: 1.4%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [43] "  G: 65.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [44] "  T: 12.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [45] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [46] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [47] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [48] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [49] "5\t1\t4.9\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [50] "12\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [51] "16\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [52] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [53] "26\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [54] "35\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [55] "36\t9\t0.0\t2\t9"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [56] "37\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [57] "41\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [58] "45\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [59] "55\t3\t0.0\t2\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [60] "56\t2\t0.0\t2\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [61] "59\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [62] "60\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [63] "61\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [64] "62\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [65] "72\t5\t0.0\t2\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [66] "74\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [67] "75\t5\t0.0\t2\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [68] "76\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [69] "79\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [70] "82\t7\t0.0\t2\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [71] "90\t7\t0.0\t2\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [72] "93\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [73] "103\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [74] "106\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [75] "122\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [76] "132\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [77] "136\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [78] "142\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [79] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [80] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [81] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [82] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [83] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [84] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [85] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [86] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [87] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 196 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [88] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [89] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [90] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [91] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [92] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [93] "  A: 76.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [94] "  C: 16.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [95] "  G: 3.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [96] "  T: 3.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [97] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [98] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [99] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [100] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [101] "5\t4\t4.9\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [102] "6\t2\t1.2\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [103] "8\t1\t0.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [104] "9\t1\t0.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [105] "11\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [106] "12\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [107] "14\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [108] "16\t6\t0.0\t1\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [109] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [110] "20\t2\t0.0\t2\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [111] "21\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [112] "22\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [113] "23\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [114] "26\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [115] "30\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [116] "32\t4\t0.0\t3\t3 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [117] "33\t3\t0.0\t3\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [118] "35\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [119] "36\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [120] "39\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [121] "40\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [122] "42\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [123] "43\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [124] "44\t5\t0.0\t4\t3 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [125] "45\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [126] "47\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [127] "49\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [128] "51\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [129] "52\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [130] "53\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [131] "54\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [132] "56\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [133] "58\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [134] "62\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [135] "63\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [136] "64\t12\t0.0\t4\t10 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [137] "65\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [138] "66\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [139] "68\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [140] "69\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [141] "73\t5\t0.0\t4\t3 1 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [142] "74\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [143] "75\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [144] "76\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [145] "77\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [146] "78\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [147] "80\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [148] "83\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [149] "84\t2\t0.0\t4\t1 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [150] "85\t3\t0.0\t4\t2 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [151] "86\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [152] "87\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [153] "88\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [154] "89\t2\t0.0\t4\t1 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [155] "90\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [156] "94\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [157] "95\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [158] "96\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [159] "97\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [160] "99\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [161] "100\t6\t0.0\t4\t4 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [162] "101\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [163] "102\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [164] "103\t9\t0.0\t4\t6 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [165] "104\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [166] "105\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [167] "106\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [168] "107\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [169] "108\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [170] "109\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [171] "110\t8\t0.0\t4\t8"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [172] "114\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [173] "115\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [174] "117\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [175] "118\t8\t0.0\t4\t7 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [176] "119\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [177] "120\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [178] "127\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [179] "130\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [180] "131\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [181] "132\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [182] "134\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [183] "135\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [184] "142\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [185] "148\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [186] "149\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [187] "150\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [188] "155\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [189] "156\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [190] "160\t1\t0.0\t4\t0 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [191] "164\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [192] "170\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [193] "171\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [194] "175\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## 
## [[41]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/188RNA6_S41_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/188RNA6_S41_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/188RNA6_S41_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/188RNA6_S41_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##   [4] "Finished in 1.20 s (117 us/read; 0.51 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [8] "Total read pairs processed:             10,268"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##   [9] "  Read 1 with adapter:                      41 (0.4%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [10] "  Read 2 with adapter:                     181 (1.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [11] "Pairs written (passing filters):        10,267 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [13] "Total basepairs processed:     4,312,560 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [14] "  Read 1:     2,012,528 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [15] "  Read 2:     2,300,032 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [16] "Total written (filtered):      4,298,819 bp (99.7%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [17] "  Read 1:     2,010,217 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [18] "  Read 2:     2,288,602 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 3 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [29] "22\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [30] "29\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [31] "32\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [32] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [34] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [36] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 38 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [38] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [39] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [40] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [41] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [42] "  A: 31.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [43] "  C: 2.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [44] "  G: 63.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [45] "  T: 2.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [46] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [47] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [48] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [49] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [50] "8\t2\t0.2\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [51] "10\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [52] "15\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [53] "17\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [54] "18\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [55] "28\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [56] "36\t5\t0.0\t2\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [57] "39\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [58] "45\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [59] "47\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [60] "49\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [61] "60\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [62] "72\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [63] "73\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [64] "76\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [65] "78\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [66] "82\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [67] "90\t7\t0.0\t2\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [68] "93\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [69] "100\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [70] "109\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [71] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [72] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [73] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [74] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [75] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [76] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [77] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [78] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [79] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 181 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [80] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [81] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [82] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [83] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [84] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [85] "  A: 79.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [86] "  C: 17.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [87] "  G: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [88] "  T: 2.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [89] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [90] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [91] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [92] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [93] "5\t9\t10.0\t0\t9"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [94] "6\t3\t2.5\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [95] "8\t5\t0.2\t0\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [96] "9\t2\t0.0\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [97] "12\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [98] "13\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [99] "14\t4\t0.0\t1\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [100] "15\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [101] "16\t2\t0.0\t1\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [102] "17\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [103] "19\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [104] "20\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [105] "22\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [106] "23\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [107] "24\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [108] "25\t8\t0.0\t2\t7 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [109] "27\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [110] "29\t2\t0.0\t2\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [111] "30\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [112] "31\t3\t0.0\t3\t1 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [113] "33\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [114] "34\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [115] "35\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [116] "36\t4\t0.0\t3\t1 2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [117] "38\t2\t0.0\t3\t0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [118] "40\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [119] "41\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [120] "43\t1\t0.0\t4\t0 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [121] "44\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [122] "45\t4\t0.0\t4\t2 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [123] "46\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [124] "47\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [125] "48\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [126] "51\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [127] "52\t3\t0.0\t4\t2 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [128] "56\t2\t0.0\t4\t1 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [129] "57\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [130] "58\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [131] "63\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [132] "64\t7\t0.0\t4\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [133] "67\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [134] "69\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [135] "73\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [136] "74\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [137] "75\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [138] "76\t2\t0.0\t4\t1 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [139] "77\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [140] "78\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [141] "79\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [142] "80\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [143] "83\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [144] "85\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [145] "86\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [146] "88\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [147] "89\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [148] "91\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [149] "92\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [150] "93\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [151] "94\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [152] "95\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [153] "96\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [154] "98\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [155] "100\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [156] "101\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [157] "103\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [158] "104\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [159] "105\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [160] "106\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [161] "107\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [162] "110\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [163] "116\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [164] "117\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [165] "118\t8\t0.0\t4\t8"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [166] "121\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [167] "127\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [168] "128\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [169] "129\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [170] "132\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [171] "133\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [172] "137\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [173] "144\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [174] "148\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [175] "152\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [176] "158\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [177] "160\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [178] "166\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [179] "173\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [180] "189\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## 
## [[42]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/188RNA7_S42_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/188RNA7_S42_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/188RNA7_S42_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/188RNA7_S42_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##   [4] "Finished in 1.29 s (120 us/read; 0.50 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [8] "Total read pairs processed:             10,772"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##   [9] "  Read 1 with adapter:                      53 (0.5%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [10] "  Read 2 with adapter:                     241 (2.2%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [11] "Pairs written (passing filters):        10,772 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [13] "Total basepairs processed:     4,524,240 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [14] "  Read 1:     2,111,312 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [15] "  Read 2:     2,412,928 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [16] "Total written (filtered):      4,506,003 bp (99.6%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [17] "  Read 1:     2,108,699 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [18] "  Read 2:     2,397,304 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 3 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [29] "7\t1\t0.7\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [30] "29\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [31] "32\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [32] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [34] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [36] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 50 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [38] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [39] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [40] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [41] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [42] "  A: 16.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [43] "  C: 6.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [44] "  G: 72.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [45] "  T: 6.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [46] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [47] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [48] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [49] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [50] "5\t1\t10.5\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [51] "6\t2\t2.6\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [52] "9\t1\t0.0\t0\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [53] "15\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [54] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [55] "36\t21\t0.0\t2\t20 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [56] "38\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [57] "41\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [58] "44\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [59] "60\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [60] "70\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [61] "71\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [62] "73\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [63] "74\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [64] "75\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [65] "82\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [66] "83\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [67] "89\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [68] "90\t6\t0.0\t2\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [69] "94\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [70] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [71] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [72] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [73] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [74] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [75] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [76] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [77] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [78] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [79] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [80] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [81] "100\t1\t0.0\t2\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [82] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [83] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [84] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [85] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [86] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 240 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [87] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [88] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [89] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [90] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [91] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [92] "  A: 82.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [93] "  C: 15.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [94] "  G: 1.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [95] "  T: 1.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [96] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [97] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [98] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [99] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [100] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [101] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [102] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [103] "5\t2\t10.5\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [104] "6\t3\t2.6\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [105] "8\t2\t0.2\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [106] "9\t4\t0.0\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [107] "11\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [108] "12\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [109] "13\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [110] "14\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [111] "15\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [112] "16\t4\t0.0\t1\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [113] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [114] "20\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [115] "22\t5\t0.0\t2\t2 2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [116] "23\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [117] "24\t3\t0.0\t2\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [118] "25\t8\t0.0\t2\t5 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [119] "26\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [120] "27\t7\t0.0\t2\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [121] "28\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [122] "29\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [123] "30\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [124] "31\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [125] "32\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [126] "33\t2\t0.0\t3\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [127] "34\t4\t0.0\t3\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [128] "35\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [129] "36\t3\t0.0\t3\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [130] "37\t1\t0.0\t3\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [131] "38\t3\t0.0\t3\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [132] "39\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [133] "40\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [134] "41\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [135] "43\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [136] "44\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [137] "45\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [138] "46\t6\t0.0\t4\t4 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [139] "47\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [140] "48\t2\t0.0\t4\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [141] "49\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [142] "50\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [143] "52\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [144] "53\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [145] "54\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [146] "57\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [147] "58\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [148] "59\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [149] "60\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [150] "62\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [151] "63\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [152] "64\t25\t0.0\t4\t21 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [153] "65\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [154] "66\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [155] "67\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [156] "68\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [157] "69\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [158] "70\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [159] "72\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [160] "74\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [161] "75\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [162] "76\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [163] "79\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [164] "81\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [165] "82\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [166] "83\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [167] "84\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [168] "85\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [169] "86\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [170] "88\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [171] "89\t3\t0.0\t4\t2 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [172] "91\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [173] "92\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [174] "93\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [175] "94\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [176] "97\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [177] "98\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [178] "99\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [179] "100\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [180] "101\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [181] "102\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [182] "103\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [183] "104\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [184] "105\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [185] "106\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [186] "108\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [187] "109\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [188] "110\t6\t0.0\t4\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [189] "115\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [190] "117\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [191] "118\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [192] "122\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [193] "123\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [194] "125\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [195] "127\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [196] "131\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [197] "132\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [198] "144\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [199] "145\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [200] "146\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [201] "150\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [202] "153\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [203] "155\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [204] "165\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [205] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [206] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [207] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [208] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [209] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## 
## [[43]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/41_S43_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/41_S43_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/41_S43_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/41_S43_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##   [4] "Finished in 1.00 s (112 us/read; 0.54 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##   [8] "Total read pairs processed:              8,960"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [9] "  Read 1 with adapter:                       7 (0.1%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [10] "  Read 2 with adapter:                     157 (1.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [11] "Pairs written (passing filters):         8,960 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [13] "Total basepairs processed:     3,763,200 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [14] "  Read 1:     1,756,160 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [15] "  Read 2:     2,007,040 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [16] "Total written (filtered):      3,753,175 bp (99.7%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [17] "  Read 1:     1,755,805 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [18] "  Read 2:     1,997,370 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 2 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [29] "9\t1\t0.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [30] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [32] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [33] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [34] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [35] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 5 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [36] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [37] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [38] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [39] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [40] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [41] "  A: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [42] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [43] "  G: 80.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [44] "  T: 20.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [45] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [46] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [47] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [48] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [49] "5\t2\t8.8\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [50] "86\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [51] "96\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [52] "137\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [53] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [54] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [55] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [56] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [57] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [58] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [59] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [60] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [61] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [62] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [63] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [64] "9\t1\t0.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [65] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [66] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [67] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [68] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [69] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 156 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [70] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [71] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [72] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [73] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [74] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [75] "  A: 84.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [76] "  C: 9.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [77] "  G: 1.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [78] "  T: 4.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [79] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [80] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [81] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [82] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [83] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [84] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [85] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [86] "5\t4\t8.8\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [87] "6\t1\t2.2\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [88] "8\t5\t0.1\t0\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [89] "9\t1\t0.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [90] "10\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [91] "11\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [92] "12\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [93] "14\t7\t0.0\t1\t6 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [94] "15\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [95] "17\t5\t0.0\t1\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [96] "19\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [97] "22\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [98] "23\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [99] "26\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [100] "27\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [101] "29\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [102] "30\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [103] "31\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [104] "32\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [105] "33\t4\t0.0\t3\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [106] "34\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [107] "37\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [108] "38\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [109] "40\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [110] "41\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [111] "42\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [112] "43\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [113] "45\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [114] "47\t2\t0.0\t4\t0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [115] "50\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [116] "51\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [117] "52\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [118] "53\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [119] "54\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [120] "55\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [121] "56\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [122] "57\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [123] "58\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [124] "59\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [125] "63\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [126] "64\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [127] "65\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [128] "66\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [129] "67\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [130] "68\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [131] "69\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [132] "70\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [133] "71\t5\t0.0\t4\t4 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [134] "73\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [135] "74\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [136] "76\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [137] "77\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [138] "78\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [139] "79\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [140] "81\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [141] "83\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [142] "84\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [143] "85\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [144] "86\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [145] "87\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [146] "88\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [147] "89\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [148] "92\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [149] "93\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [150] "94\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [151] "95\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [152] "96\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [153] "102\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [154] "104\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [155] "107\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [156] "109\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [157] "111\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [158] "113\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [159] "114\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [160] "117\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [161] "122\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [162] "124\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [163] "129\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [164] "132\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [165] "134\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [166] "137\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [167] "138\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [168] "144\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [169] "145\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [170] "150\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [171] "152\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [172] "160\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [173] "162\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [174] "163\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [175] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [176] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [177] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [178] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [179] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## 
## [[44]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/42_S44_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/42_S44_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/42_S44_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/42_S44_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##   [4] "Finished in 1.32 s (112 us/read; 0.54 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##   [8] "Total read pairs processed:             11,867"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [9] "  Read 1 with adapter:                       6 (0.1%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [10] "  Read 2 with adapter:                     176 (1.5%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [11] "Pairs written (passing filters):        11,867 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [13] "Total basepairs processed:     4,984,140 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [14] "  Read 1:     2,325,932 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [15] "  Read 2:     2,658,208 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [16] "Total written (filtered):      4,973,138 bp (99.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [17] "  Read 1:     2,325,692 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [18] "  Read 2:     2,647,446 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 3 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [29] "5\t1\t11.6\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [30] "6\t2\t2.9\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [32] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [33] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [34] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [35] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 3 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [36] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [37] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [38] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [39] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [40] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [41] "  A: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [42] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [43] "  G: 100.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [44] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [45] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [46] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [47] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [48] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [49] "43\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [50] "90\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [51] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [52] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [53] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [54] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [55] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [56] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [57] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [58] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [59] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 176 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [60] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [61] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [62] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [63] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [64] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [65] "  A: 82.4%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [66] "  C: 13.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [67] "  G: 1.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [68] "  T: 2.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [69] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [70] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [71] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [72] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [73] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [74] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [75] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [76] "5\t2\t11.6\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [77] "6\t3\t2.9\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [78] "7\t1\t0.7\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [79] "8\t3\t0.2\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [80] "9\t1\t0.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [81] "10\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [82] "11\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [83] "12\t3\t0.0\t1\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [84] "14\t8\t0.0\t1\t8"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [85] "16\t8\t0.0\t1\t8"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [86] "17\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [87] "19\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [88] "20\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [89] "22\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [90] "23\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [91] "24\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [92] "25\t1\t0.0\t2\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [93] "27\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [94] "28\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [95] "29\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [96] "30\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [97] "32\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [98] "33\t3\t0.0\t3\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [99] "34\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [100] "35\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [101] "36\t3\t0.0\t3\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [102] "37\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [103] "38\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [104] "39\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [105] "40\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [106] "41\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [107] "42\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [108] "43\t6\t0.0\t4\t3 2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [109] "45\t1\t0.0\t4\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [110] "46\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [111] "47\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [112] "48\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [113] "51\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [114] "52\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [115] "54\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [116] "55\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [117] "57\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [118] "58\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [119] "59\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [120] "60\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [121] "63\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [122] "64\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [123] "66\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [124] "71\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [125] "72\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [126] "73\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [127] "74\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [128] "75\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [129] "81\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [130] "82\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [131] "83\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [132] "84\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [133] "85\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [134] "86\t7\t0.0\t4\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [135] "93\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [136] "94\t2\t0.0\t4\t0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [137] "96\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [138] "97\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [139] "98\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [140] "99\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [141] "103\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [142] "104\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [143] "105\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [144] "106\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [145] "109\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [146] "110\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [147] "111\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [148] "112\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [149] "113\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [150] "114\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [151] "115\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [152] "116\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [153] "118\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [154] "119\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [155] "120\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [156] "121\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [157] "126\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [158] "131\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [159] "133\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [160] "134\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [161] "136\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [162] "138\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [163] "140\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [164] "141\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [165] "157\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [166] "158\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [167] "172\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [168] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [169] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [170] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [171] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [172] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## 
## [[45]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/551_S45_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/551_S45_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/551_S45_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/551_S45_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##   [4] "Finished in 1.30 s (114 us/read; 0.53 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [8] "Total read pairs processed:             11,417"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##   [9] "  Read 1 with adapter:                       4 (0.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [10] "  Read 2 with adapter:                     105 (0.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [11] "Pairs written (passing filters):        11,417 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [13] "Total basepairs processed:     4,795,140 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [14] "  Read 1:     2,237,732 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [15] "  Read 2:     2,557,408 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [16] "Total written (filtered):      4,788,933 bp (99.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [17] "  Read 1:     2,237,680 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [18] "  Read 2:     2,551,253 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 4 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [29] "6\t1\t2.8\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [30] "12\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [31] "17\t2\t0.0\t1\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [32] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [34] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [36] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [38] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [39] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [40] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [41] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [42] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [43] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [44] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 105 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [45] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [46] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [47] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [48] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [49] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [50] "  A: 67.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [51] "  C: 25.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [52] "  G: 3.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [53] "  T: 2.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [54] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [55] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [56] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [57] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [58] "5\t7\t11.1\t0\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [59] "6\t1\t2.8\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [60] "7\t1\t0.7\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [61] "8\t1\t0.2\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [62] "12\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [63] "14\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [64] "15\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [65] "16\t8\t0.0\t1\t7 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [66] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [67] "18\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [68] "19\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [69] "20\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [70] "22\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [71] "25\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [72] "26\t2\t0.0\t2\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [73] "27\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [74] "28\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [75] "29\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [76] "31\t3\t0.0\t3\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [77] "34\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [78] "35\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [79] "39\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [80] "41\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [81] "42\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [82] "44\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [83] "47\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [84] "48\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [85] "50\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [86] "53\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [87] "54\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [88] "57\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [89] "58\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [90] "59\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [91] "62\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [92] "63\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [93] "65\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [94] "67\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [95] "72\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [96] "73\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [97] "76\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [98] "79\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [99] "82\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [100] "84\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [101] "86\t2\t0.0\t4\t0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [102] "87\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [103] "88\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [104] "89\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [105] "96\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [106] "98\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [107] "102\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [108] "103\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [109] "104\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [110] "105\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [111] "113\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [112] "115\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [113] "118\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [114] "119\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [115] "121\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [116] "127\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [117] "133\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [118] "140\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [119] "143\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [120] "146\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [121] "149\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [122] "153\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## 
## [[46]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/552_S46_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/552_S46_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/552_S46_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/552_S46_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##   [4] "Finished in 1.59 s (116 us/read; 0.52 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [8] "Total read pairs processed:             13,753"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##   [9] "  Read 1 with adapter:                       6 (0.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [10] "  Read 2 with adapter:                     185 (1.3%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [11] "Pairs written (passing filters):        13,753 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [13] "Total basepairs processed:     5,776,260 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [14] "  Read 1:     2,695,588 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [15] "  Read 2:     3,080,672 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [16] "Total written (filtered):      5,764,090 bp (99.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [17] "  Read 1:     2,695,421 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [18] "  Read 2:     3,068,669 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 3 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [29] "5\t1\t13.4\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [30] "11\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [31] "29\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [32] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [34] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [36] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 3 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [38] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [39] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [40] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [41] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [42] "  A: 66.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [43] "  C: 33.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [44] "  G: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [45] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [46] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [47] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [48] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [49] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [50] "16\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [51] "42\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [52] "64\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [53] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [54] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [55] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [56] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [57] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 4 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [58] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [59] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [60] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [61] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [62] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [63] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [64] "5\t2\t13.4\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [65] "12\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [66] "25\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [67] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [68] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [69] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [70] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [71] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 183 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [72] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [73] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [74] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [75] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [76] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [77] "  A: 63.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [78] "  C: 33.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [79] "  G: 0.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [80] "  T: 2.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [81] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [82] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [83] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [84] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [85] "5\t7\t13.4\t0\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [86] "6\t3\t3.4\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [87] "7\t2\t0.8\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [88] "8\t4\t0.2\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [89] "10\t2\t0.0\t1\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [90] "12\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [91] "13\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [92] "14\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [93] "16\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [94] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [95] "18\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [96] "19\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [97] "20\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [98] "22\t7\t0.0\t2\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [99] "23\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [100] "24\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [101] "25\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [102] "28\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [103] "31\t3\t0.0\t3\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [104] "32\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [105] "33\t3\t0.0\t3\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [106] "34\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [107] "35\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [108] "36\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [109] "37\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [110] "38\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [111] "40\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [112] "41\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [113] "42\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [114] "44\t2\t0.0\t4\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [115] "45\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [116] "46\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [117] "47\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [118] "48\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [119] "49\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [120] "50\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [121] "51\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [122] "52\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [123] "54\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [124] "57\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [125] "58\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [126] "59\t2\t0.0\t4\t0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [127] "60\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [128] "62\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [129] "64\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [130] "65\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [131] "66\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [132] "69\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [133] "70\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [134] "71\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [135] "72\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [136] "73\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [137] "74\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [138] "77\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [139] "78\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [140] "79\t7\t0.0\t4\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [141] "80\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [142] "82\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [143] "83\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [144] "84\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [145] "85\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [146] "86\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [147] "87\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [148] "88\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [149] "89\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [150] "90\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [151] "92\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [152] "93\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [153] "94\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [154] "95\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [155] "99\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [156] "100\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [157] "101\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [158] "102\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [159] "103\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [160] "104\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [161] "105\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [162] "106\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [163] "108\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [164] "111\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [165] "112\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [166] "113\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [167] "114\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [168] "115\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [169] "118\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [170] "120\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [171] "121\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [172] "122\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [173] "123\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [174] "124\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [175] "126\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [176] "127\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [177] "136\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [178] "138\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [179] "143\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [180] "145\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [181] "148\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [182] "150\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [183] "154\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [184] "156\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [185] "159\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [186] "174\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## 
## [[47]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/553_S47_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/553_S47_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/553_S47_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/553_S47_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##   [4] "Finished in 1.41 s (115 us/read; 0.52 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [8] "Total read pairs processed:             12,254"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##   [9] "  Read 1 with adapter:                       5 (0.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [10] "  Read 2 with adapter:                     139 (1.1%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [11] "Pairs written (passing filters):        12,253 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [13] "Total basepairs processed:     5,146,680 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [14] "  Read 1:     2,401,784 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [15] "  Read 2:     2,744,896 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [16] "Total written (filtered):      5,138,010 bp (99.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [17] "  Read 1:     2,401,375 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [18] "  Read 2:     2,736,635 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 4 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [29] "9\t1\t0.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [30] "14\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [31] "21\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [32] "25\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [34] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [35] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [36] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [37] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [39] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [40] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [41] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [42] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [43] "  A: 100.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [44] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [45] "  G: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [46] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [47] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [48] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [49] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [50] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [51] "144\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [52] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [53] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [54] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [55] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [56] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [57] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [58] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [59] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [60] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [61] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [62] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [63] "18\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [64] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [65] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [66] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [67] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [68] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 138 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [69] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [70] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [71] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [72] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [73] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [74] "  A: 79.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [75] "  C: 17.4%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [76] "  G: 2.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [77] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [78] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [79] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [80] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [81] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [82] "5\t7\t12.0\t0\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [83] "6\t1\t3.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [84] "7\t1\t0.7\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [85] "8\t4\t0.2\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [86] "12\t4\t0.0\t1\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [87] "14\t6\t0.0\t1\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [88] "15\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [89] "16\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [90] "17\t3\t0.0\t1\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [91] "19\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [92] "20\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [93] "21\t3\t0.0\t2\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [94] "23\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [95] "24\t3\t0.0\t2\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [96] "25\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [97] "26\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [98] "30\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [99] "31\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [100] "32\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [101] "33\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [102] "34\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [103] "35\t4\t0.0\t3\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [104] "37\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [105] "38\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [106] "39\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [107] "40\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [108] "41\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [109] "42\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [110] "43\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [111] "44\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [112] "47\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [113] "48\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [114] "51\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [115] "52\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [116] "53\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [117] "55\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [118] "59\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [119] "60\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [120] "61\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [121] "62\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [122] "63\t2\t0.0\t4\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [123] "64\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [124] "65\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [125] "70\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [126] "71\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [127] "74\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [128] "79\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [129] "80\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [130] "84\t2\t0.0\t4\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [131] "85\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [132] "87\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [133] "89\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [134] "92\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [135] "93\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [136] "94\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [137] "98\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [138] "100\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [139] "101\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [140] "102\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [141] "103\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [142] "104\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [143] "105\t7\t0.0\t4\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [144] "106\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [145] "107\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [146] "109\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [147] "110\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [148] "111\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [149] "116\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [150] "120\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [151] "121\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [152] "125\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [153] "126\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [154] "127\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [155] "132\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [156] "150\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [157] "151\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [158] "160\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [159] "172\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [160] "179\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## 
## [[48]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/554_S48_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/554_S48_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/554_S48_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/554_S48_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##   [4] "Finished in 1.46 s (113 us/read; 0.53 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [8] "Total read pairs processed:             12,965"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##   [9] "  Read 1 with adapter:                       4 (0.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [10] "  Read 2 with adapter:                     122 (0.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [11] "Pairs written (passing filters):        12,964 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [13] "Total basepairs processed:     5,445,300 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [14] "  Read 1:     2,541,140 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [15] "  Read 2:     2,904,160 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [16] "Total written (filtered):      5,437,236 bp (99.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [17] "  Read 1:     2,540,750 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [18] "  Read 2:     2,896,486 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 3 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [29] "8\t1\t0.2\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [30] "18\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [31] "28\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [32] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [34] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [36] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [38] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [39] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [40] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [41] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [42] "  A: 100.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [43] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [44] "  G: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [45] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [46] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [47] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [48] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [49] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [50] "140\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [51] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [52] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [53] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [54] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [55] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 2 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [56] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [57] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [58] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [59] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [60] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [61] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [62] "5\t2\t12.7\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [63] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [64] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [65] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [66] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [67] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 122 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [68] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [69] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [70] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [71] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [72] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [73] "  A: 91.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [74] "  C: 5.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [75] "  G: 2.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [76] "  T: 0.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [77] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [78] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [79] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [80] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [81] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [82] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [83] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [84] "5\t2\t12.7\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [85] "6\t1\t3.2\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [86] "7\t1\t0.8\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [87] "8\t2\t0.2\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [88] "10\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [89] "11\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [90] "14\t2\t0.0\t1\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [91] "16\t4\t0.0\t1\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [92] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [93] "19\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [94] "20\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [95] "21\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [96] "22\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [97] "23\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [98] "27\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [99] "28\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [100] "29\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [101] "30\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [102] "31\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [103] "33\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [104] "34\t3\t0.0\t3\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [105] "35\t3\t0.0\t3\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [106] "36\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [107] "37\t2\t0.0\t3\t1 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [108] "39\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [109] "41\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [110] "42\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [111] "43\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [112] "44\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [113] "45\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [114] "47\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [115] "48\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [116] "49\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [117] "51\t3\t0.0\t4\t1 0 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [118] "52\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [119] "53\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [120] "54\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [121] "55\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [122] "58\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [123] "60\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [124] "62\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [125] "63\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [126] "64\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [127] "65\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [128] "66\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [129] "71\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [130] "72\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [131] "73\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [132] "74\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [133] "75\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [134] "76\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [135] "78\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [136] "79\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [137] "83\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [138] "84\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [139] "86\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [140] "90\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [141] "91\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [142] "93\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [143] "94\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [144] "95\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [145] "98\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [146] "100\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [147] "101\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [148] "104\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [149] "105\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [150] "107\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [151] "108\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [152] "113\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [153] "119\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [154] "121\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [155] "123\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [156] "124\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [157] "130\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [158] "143\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [159] "148\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [160] "150\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [161] "160\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [162] "176\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [163] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [164] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [165] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [166] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [167] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## 
## [[49]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/55RNA1_S49_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/55RNA1_S49_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/55RNA1_S49_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/55RNA1_S49_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##   [4] "Finished in 0.91 s (116 us/read; 0.52 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##   [8] "Total read pairs processed:              7,835"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [9] "  Read 1 with adapter:                      38 (0.5%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [10] "  Read 2 with adapter:                     234 (3.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [11] "Pairs written (passing filters):         7,834 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [13] "Total basepairs processed:     3,290,700 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [14] "  Read 1:     1,535,660 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [15] "  Read 2:     1,755,040 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [16] "Total written (filtered):      3,271,489 bp (99.4%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [17] "  Read 1:     1,533,204 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [18] "  Read 2:     1,738,285 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 2 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [29] "5\t1\t7.7\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [30] "14\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [32] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [33] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [34] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [35] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 36 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [36] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [37] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [38] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [39] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [40] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [41] "  A: 16.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [42] "  C: 11.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [43] "  G: 72.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [44] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [45] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [46] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [47] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [48] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [49] "5\t1\t7.7\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [50] "6\t1\t1.9\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [51] "9\t2\t0.0\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [52] "12\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [53] "16\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [54] "23\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [55] "27\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [56] "35\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [57] "36\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [58] "64\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [59] "81\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [60] "82\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [61] "83\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [62] "90\t15\t0.0\t2\t15"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [63] "109\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [64] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [65] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [66] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [67] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [68] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [69] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [70] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [71] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [72] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 234 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [73] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [74] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [75] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [76] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [77] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [78] "  A: 82.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [79] "  C: 12.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [80] "  G: 2.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [81] "  T: 3.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [82] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [83] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [84] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [85] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [86] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [87] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [88] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [89] "5\t2\t7.7\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [90] "6\t5\t1.9\t0\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [91] "7\t3\t0.5\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [92] "8\t6\t0.1\t0\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [93] "11\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [94] "12\t3\t0.0\t1\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [95] "13\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [96] "14\t6\t0.0\t1\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [97] "15\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [98] "16\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [99] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [100] "19\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [101] "21\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [102] "22\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [103] "23\t3\t0.0\t2\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [104] "24\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [105] "25\t3\t0.0\t2\t0 2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [106] "26\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [107] "27\t6\t0.0\t2\t5 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [108] "28\t2\t0.0\t2\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [109] "29\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [110] "30\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [111] "32\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [112] "33\t4\t0.0\t3\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [113] "34\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [114] "35\t3\t0.0\t3\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [115] "36\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [116] "37\t2\t0.0\t3\t0 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [117] "39\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [118] "40\t5\t0.0\t4\t2 2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [119] "42\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [120] "43\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [121] "44\t6\t0.0\t4\t4 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [122] "45\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [123] "46\t3\t0.0\t4\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [124] "47\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [125] "50\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [126] "51\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [127] "52\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [128] "53\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [129] "55\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [130] "56\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [131] "58\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [132] "59\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [133] "60\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [134] "62\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [135] "63\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [136] "64\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [137] "66\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [138] "67\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [139] "69\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [140] "71\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [141] "74\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [142] "75\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [143] "76\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [144] "79\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [145] "82\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [146] "84\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [147] "85\t6\t0.0\t4\t5 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [148] "86\t7\t0.0\t4\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [149] "87\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [150] "88\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [151] "90\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [152] "92\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [153] "93\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [154] "94\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [155] "95\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [156] "98\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [157] "100\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [158] "101\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [159] "102\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [160] "103\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [161] "105\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [162] "107\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [163] "108\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [164] "109\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [165] "110\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [166] "111\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [167] "112\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [168] "114\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [169] "116\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [170] "117\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [171] "118\t15\t0.0\t4\t13 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [172] "119\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [173] "121\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [174] "124\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [175] "125\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [176] "126\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [177] "128\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [178] "130\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [179] "132\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [180] "134\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [181] "135\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [182] "137\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [183] "138\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [184] "139\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [185] "145\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [186] "147\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [187] "148\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [188] "152\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [189] "153\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [190] "158\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [191] "159\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [192] "160\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [193] "171\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [194] "178\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [195] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [196] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [197] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [198] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [199] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## 
## [[50]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/55RNA2_S50_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/55RNA2_S50_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/55RNA2_S50_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/55RNA2_S50_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##   [4] "Finished in 1.06 s (113 us/read; 0.53 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##   [8] "Total read pairs processed:              9,385"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [9] "  Read 1 with adapter:                      55 (0.6%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [10] "  Read 2 with adapter:                     354 (3.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [11] "Pairs written (passing filters):         9,385 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [13] "Total basepairs processed:     3,941,700 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [14] "  Read 1:     1,839,460 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [15] "  Read 2:     2,102,240 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [16] "Total written (filtered):      3,911,285 bp (99.2%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [17] "  Read 1:     1,835,958 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [18] "  Read 2:     2,075,327 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 3 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [29] "7\t1\t0.6\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [30] "8\t1\t0.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [31] "26\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [32] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [34] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [36] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 52 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [38] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [39] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [40] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [41] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [42] "  A: 9.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [43] "  C: 7.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [44] "  G: 82.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [45] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [46] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [47] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [48] "    The adapter is preceded by \"G\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [49] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [50] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [51] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [52] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [53] "5\t1\t9.2\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [54] "7\t1\t0.6\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [55] "8\t1\t0.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [56] "9\t1\t0.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [57] "11\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [58] "12\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [59] "16\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [60] "17\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [61] "18\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [62] "26\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [63] "36\t5\t0.0\t2\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [64] "41\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [65] "61\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [66] "69\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [67] "77\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [68] "82\t5\t0.0\t2\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [69] "90\t19\t0.0\t2\t18 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [70] "109\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [71] "123\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [72] "131\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [73] "143\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [74] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [75] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [76] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [77] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [78] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [79] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [80] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [81] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [82] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [83] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [84] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [85] "8\t1\t0.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [86] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [87] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [88] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [89] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [90] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 353 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [91] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [92] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [93] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [94] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [95] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [96] "  A: 80.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [97] "  C: 15.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [98] "  G: 1.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [99] "  T: 3.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [100] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [101] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [102] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [103] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [104] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [105] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [106] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [107] "5\t2\t9.2\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [108] "6\t1\t2.3\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [109] "7\t1\t0.6\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [110] "8\t3\t0.1\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [111] "10\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [112] "11\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [113] "12\t3\t0.0\t1\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [114] "13\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [115] "14\t8\t0.0\t1\t7 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [116] "15\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [117] "16\t8\t0.0\t1\t8"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [118] "18\t3\t0.0\t1\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [119] "19\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [120] "20\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [121] "21\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [122] "22\t4\t0.0\t2\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [123] "23\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [124] "24\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [125] "25\t3\t0.0\t2\t1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [126] "26\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [127] "27\t7\t0.0\t2\t4 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [128] "28\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [129] "30\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [130] "31\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [131] "32\t4\t0.0\t3\t2 1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [132] "33\t4\t0.0\t3\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [133] "34\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [134] "35\t5\t0.0\t3\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [135] "36\t5\t0.0\t3\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [136] "37\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [137] "38\t2\t0.0\t3\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [138] "39\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [139] "40\t3\t0.0\t4\t0 1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [140] "41\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [141] "42\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [142] "43\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [143] "44\t2\t0.0\t4\t1 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [144] "45\t6\t0.0\t4\t4 1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [145] "46\t2\t0.0\t4\t1 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [146] "50\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [147] "51\t2\t0.0\t4\t1 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [148] "52\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [149] "53\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [150] "54\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [151] "55\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [152] "57\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [153] "58\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [154] "59\t5\t0.0\t4\t4 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [155] "60\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [156] "61\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [157] "62\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [158] "63\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [159] "64\t10\t0.0\t4\t9 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [160] "65\t10\t0.0\t4\t9 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [161] "66\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [162] "67\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [163] "69\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [164] "72\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [165] "73\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [166] "74\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [167] "75\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [168] "77\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [169] "78\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [170] "79\t4\t0.0\t4\t3 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [171] "81\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [172] "82\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [173] "83\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [174] "84\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [175] "85\t10\t0.0\t4\t9 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [176] "86\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [177] "87\t7\t0.0\t4\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [178] "88\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [179] "89\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [180] "90\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [181] "91\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [182] "92\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [183] "93\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [184] "94\t7\t0.0\t4\t5 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [185] "95\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [186] "96\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [187] "97\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [188] "98\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [189] "99\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [190] "101\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [191] "102\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [192] "103\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [193] "104\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [194] "105\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [195] "106\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [196] "108\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [197] "109\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [198] "110\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [199] "116\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [200] "117\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [201] "118\t20\t0.0\t4\t16 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [202] "119\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [203] "121\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [204] "122\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [205] "125\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [206] "126\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [207] "127\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [208] "128\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [209] "129\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [210] "132\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [211] "134\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [212] "136\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [213] "137\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [214] "138\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [215] "141\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [216] "142\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [217] "144\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [218] "145\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [219] "146\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [220] "147\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [221] "150\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [222] "151\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [223] "152\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [224] "159\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [225] "160\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [226] "161\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [227] "162\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [228] "165\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [229] "166\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [230] "171\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [231] "173\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [232] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [233] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [234] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [235] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [236] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## 
## [[51]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/55RNA3_S51_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/55RNA3_S51_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/55RNA3_S51_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/55RNA3_S51_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##   [4] "Finished in 0.90 s (116 us/read; 0.52 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##   [8] "Total read pairs processed:              7,778"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [9] "  Read 1 with adapter:                     170 (2.2%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [10] "  Read 2 with adapter:                     499 (6.4%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [11] "Pairs written (passing filters):         7,776 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [13] "Total basepairs processed:     3,266,760 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [14] "  Read 1:     1,524,488 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [15] "  Read 2:     1,742,272 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [16] "Total written (filtered):      3,210,979 bp (98.3%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [17] "  Read 1:     1,510,586 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [18] "  Read 2:     1,700,393 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 5 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [29] "8\t1\t0.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [30] "26\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [31] "30\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [32] "31\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [33] "65\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [34] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [36] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [38] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 165 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [39] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [40] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [41] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [42] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [43] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [44] "  A: 2.4%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [45] "  C: 1.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [46] "  G: 95.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [47] "  T: 0.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [48] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [49] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [50] "    The adapter is preceded by \"G\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [51] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [52] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [53] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [54] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [55] "10\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [56] "15\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [57] "16\t3\t0.0\t1\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [58] "17\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [59] "23\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [60] "25\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [61] "26\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [62] "36\t6\t0.0\t2\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [63] "42\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [64] "43\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [65] "49\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [66] "56\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [67] "60\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [68] "64\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [69] "66\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [70] "81\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [71] "82\t27\t0.0\t2\t27"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [72] "90\t104\t0.0\t2\t103 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [73] "91\t5\t0.0\t2\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [74] "109\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [75] "110\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [76] "125\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [77] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [78] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [79] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [80] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [81] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 2 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [82] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [83] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [84] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [85] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [86] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [87] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [88] "5\t2\t7.6\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [89] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [90] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [91] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [92] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [93] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 499 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [94] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [95] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [96] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [97] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [98] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [99] "  A: 91.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [100] "  C: 6.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [101] "  G: 0.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [102] "  T: 1.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [103] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [104] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [105] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [106] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [107] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [108] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [109] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [110] "5\t3\t7.6\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [111] "6\t3\t1.9\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [112] "8\t3\t0.1\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [113] "10\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [114] "11\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [115] "12\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [116] "14\t6\t0.0\t1\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [117] "15\t5\t0.0\t1\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [118] "16\t4\t0.0\t1\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [119] "17\t5\t0.0\t1\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [120] "18\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [121] "19\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [122] "20\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [123] "21\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [124] "22\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [125] "23\t5\t0.0\t2\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [126] "24\t3\t0.0\t2\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [127] "25\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [128] "26\t5\t0.0\t2\t3 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [129] "27\t7\t0.0\t2\t6 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [130] "28\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [131] "30\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [132] "31\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [133] "32\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [134] "33\t5\t0.0\t3\t4 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [135] "34\t3\t0.0\t3\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [136] "35\t6\t0.0\t3\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [137] "36\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [138] "38\t6\t0.0\t3\t3 2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [139] "39\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [140] "42\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [141] "43\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [142] "44\t7\t0.0\t4\t2 4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [143] "45\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [144] "46\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [145] "47\t6\t0.0\t4\t4 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [146] "48\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [147] "49\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [148] "50\t4\t0.0\t4\t3 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [149] "51\t5\t0.0\t4\t3 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [150] "52\t8\t0.0\t4\t6 1 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [151] "53\t6\t0.0\t4\t5 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [152] "54\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [153] "55\t4\t0.0\t4\t3 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [154] "56\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [155] "57\t7\t0.0\t4\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [156] "58\t5\t0.0\t4\t3 1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [157] "59\t6\t0.0\t4\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [158] "62\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [159] "63\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [160] "64\t10\t0.0\t4\t9 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [161] "65\t5\t0.0\t4\t3 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [162] "66\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [163] "67\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [164] "68\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [165] "69\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [166] "70\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [167] "71\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [168] "72\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [169] "73\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [170] "74\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [171] "75\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [172] "76\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [173] "77\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [174] "78\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [175] "79\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [176] "82\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [177] "83\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [178] "84\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [179] "85\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [180] "86\t11\t0.0\t4\t10 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [181] "87\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [182] "88\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [183] "89\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [184] "90\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [185] "92\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [186] "93\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [187] "94\t15\t0.0\t4\t15"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [188] "95\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [189] "96\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [190] "97\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [191] "98\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [192] "99\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [193] "100\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [194] "101\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [195] "103\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [196] "104\t7\t0.0\t4\t5 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [197] "105\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [198] "106\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [199] "107\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [200] "108\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [201] "109\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [202] "110\t28\t0.0\t4\t28"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [203] "112\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [204] "113\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [205] "114\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [206] "115\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [207] "116\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [208] "117\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [209] "118\t106\t0.0\t4\t98 7 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [210] "119\t3\t0.0\t4\t1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [211] "120\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [212] "121\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [213] "127\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [214] "128\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [215] "129\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [216] "130\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [217] "131\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [218] "132\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [219] "134\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [220] "136\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [221] "137\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [222] "138\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [223] "139\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [224] "144\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [225] "145\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [226] "146\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [227] "147\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [228] "150\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [229] "153\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [230] "158\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [231] "160\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [232] "161\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [233] "165\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [234] "167\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [235] "168\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [236] "173\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [237] "175\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [238] "179\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [239] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [240] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [241] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [242] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [243] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## 
## [[52]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/55RNA4_S52_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/55RNA4_S52_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/55RNA4_S52_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/55RNA4_S52_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##   [4] "Finished in 0.25 s (117 us/read; 0.51 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##   [8] "Total read pairs processed:              2,165"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [9] "  Read 1 with adapter:                      11 (0.5%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [10] "  Read 2 with adapter:                      86 (4.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [11] "Pairs written (passing filters):         2,164 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [13] "Total basepairs processed:       909,300 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [14] "  Read 1:       424,340 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [15] "  Read 2:       484,960 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [16] "Total written (filtered):        902,063 bp (99.2%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [17] "  Read 1:       423,498 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [18] "  Read 2:       478,565 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [24] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [25] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [26] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 11 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [27] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [28] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [29] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [30] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [31] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [32] "  A: 18.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [33] "  C: 9.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [34] "  G: 72.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [35] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [36] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [38] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [39] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [40] "15\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [41] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [42] "25\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [43] "36\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [44] "82\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [45] "90\t4\t0.0\t2\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [46] "111\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [47] "147\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [48] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [49] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [50] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [51] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [52] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [53] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [54] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [55] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [56] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [57] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [58] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [59] "5\t1\t2.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [60] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [61] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [62] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [63] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [64] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 86 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [65] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [66] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [67] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [68] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [69] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [70] "  A: 88.4%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [71] "  C: 7.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [72] "  G: 4.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [73] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [74] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [75] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [76] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [77] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [78] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [79] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [80] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [81] "6\t2\t0.5\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [82] "8\t1\t0.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [83] "12\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [84] "13\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [85] "24\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [86] "26\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [87] "31\t1\t0.0\t3\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [88] "34\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [89] "35\t2\t0.0\t3\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [90] "40\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [91] "42\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [92] "43\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [93] "45\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [94] "46\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [95] "50\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [96] "51\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [97] "52\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [98] "53\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [99] "55\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [100] "58\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [101] "60\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [102] "61\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [103] "62\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [104] "63\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [105] "64\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [106] "65\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [107] "66\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [108] "68\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [109] "69\t3\t0.0\t4\t2 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [110] "70\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [111] "71\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [112] "72\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [113] "73\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [114] "77\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [115] "79\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [116] "81\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [117] "84\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [118] "86\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [119] "87\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [120] "88\t4\t0.0\t4\t3 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [121] "92\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [122] "93\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [123] "94\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [124] "95\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [125] "98\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [126] "102\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [127] "104\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [128] "106\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [129] "110\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [130] "113\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [131] "115\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [132] "118\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [133] "121\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [134] "133\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [135] "136\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [136] "139\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [137] "146\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [138] "164\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [139] "175\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [140] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [141] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [142] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [143] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [144] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## 
## [[53]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/71A1_S53_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/71A1_S53_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/71A1_S53_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/71A1_S53_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##   [4] "Finished in 1.92 s (107 us/read; 0.56 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [8] "Total read pairs processed:             17,921"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [9] "  Read 1 with adapter:                       3 (0.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [10] "  Read 2 with adapter:                     132 (0.7%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [11] "Pairs written (passing filters):        17,921 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [13] "Total basepairs processed:     7,526,820 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [14] "  Read 1:     3,512,516 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [15] "  Read 2:     4,014,304 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [16] "Total written (filtered):      7,518,914 bp (99.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [17] "  Read 1:     3,512,468 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [18] "  Read 2:     4,006,446 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 3 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [29] "5\t1\t17.5\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [30] "7\t1\t1.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [31] "36\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [32] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [34] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [36] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [38] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [39] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [40] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 2 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [41] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [42] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [43] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [44] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [45] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [46] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [47] "12\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [48] "14\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [49] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [50] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [51] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [52] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [53] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 130 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [54] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [55] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [56] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [57] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [58] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [59] "  A: 62.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [60] "  C: 36.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [61] "  G: 1.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [62] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [63] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [64] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [65] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [66] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [67] "5\t3\t17.5\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [68] "6\t1\t4.4\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [69] "7\t1\t1.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [70] "9\t5\t0.1\t0\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [71] "10\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [72] "11\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [73] "12\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [74] "14\t4\t0.0\t1\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [75] "15\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [76] "16\t5\t0.0\t1\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [77] "17\t4\t0.0\t1\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [78] "18\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [79] "21\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [80] "22\t4\t0.0\t2\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [81] "25\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [82] "28\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [83] "32\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [84] "33\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [85] "34\t5\t0.0\t3\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [86] "35\t3\t0.0\t3\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [87] "38\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [88] "39\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [89] "40\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [90] "41\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [91] "42\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [92] "44\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [93] "45\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [94] "46\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [95] "48\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [96] "52\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [97] "53\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [98] "54\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [99] "55\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [100] "57\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [101] "58\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [102] "59\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [103] "65\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [104] "66\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [105] "69\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [106] "71\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [107] "72\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [108] "73\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [109] "74\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [110] "75\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [111] "76\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [112] "77\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [113] "79\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [114] "81\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [115] "82\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [116] "84\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [117] "85\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [118] "86\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [119] "87\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [120] "88\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [121] "89\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [122] "93\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [123] "97\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [124] "98\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [125] "99\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [126] "103\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [127] "106\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [128] "107\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [129] "109\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [130] "111\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [131] "112\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [132] "113\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [133] "114\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [134] "116\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [135] "121\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [136] "129\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [137] "131\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [138] "138\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [139] "140\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [140] "145\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [141] "147\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [142] "157\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [143] "160\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [144] "171\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## 
## [[54]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/71A2_S54_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/71A2_S54_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/71A2_S54_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/71A2_S54_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##   [4] "Finished in 2.27 s (109 us/read; 0.55 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [8] "Total read pairs processed:             20,847"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [9] "  Read 1 with adapter:                       8 (0.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [10] "  Read 2 with adapter:                     185 (0.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [11] "Pairs written (passing filters):        20,846 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [13] "Total basepairs processed:     8,755,740 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [14] "  Read 1:     4,086,012 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [15] "  Read 2:     4,669,728 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [16] "Total written (filtered):      8,743,381 bp (99.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [17] "  Read 1:     4,085,693 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [18] "  Read 2:     4,657,688 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 6 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [29] "7\t1\t1.3\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [30] "8\t3\t0.3\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [31] "9\t1\t0.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [32] "24\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [34] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [35] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [36] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [37] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 2 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [39] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [40] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [41] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [42] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [43] "  A: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [44] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [45] "  G: 100.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [46] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [47] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [48] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [49] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [50] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [51] "24\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [52] "35\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [53] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [54] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [55] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [56] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [57] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [58] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [59] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [60] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [61] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [62] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [63] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [64] "7\t1\t1.3\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [65] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [66] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [67] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [68] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [69] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 184 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [70] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [71] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [72] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [73] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [74] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [75] "  A: 62.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [76] "  C: 36.4%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [77] "  G: 1.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [78] "  T: 0.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [79] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [80] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [81] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [82] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [83] "5\t6\t20.4\t0\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [84] "6\t3\t5.1\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [85] "7\t2\t1.3\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [86] "8\t3\t0.3\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [87] "10\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [88] "11\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [89] "12\t4\t0.0\t1\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [90] "14\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [91] "15\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [92] "16\t2\t0.0\t1\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [93] "17\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [94] "18\t4\t0.0\t1\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [95] "19\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [96] "20\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [97] "21\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [98] "22\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [99] "23\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [100] "24\t4\t0.0\t2\t2 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [101] "25\t4\t0.0\t2\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [102] "27\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [103] "28\t2\t0.0\t2\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [104] "29\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [105] "30\t1\t0.0\t3\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [106] "31\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [107] "32\t3\t0.0\t3\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [108] "33\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [109] "34\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [110] "35\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [111] "37\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [112] "38\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [113] "39\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [114] "41\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [115] "42\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [116] "43\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [117] "45\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [118] "46\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [119] "47\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [120] "48\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [121] "50\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [122] "51\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [123] "53\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [124] "54\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [125] "55\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [126] "56\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [127] "57\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [128] "58\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [129] "60\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [130] "62\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [131] "63\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [132] "64\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [133] "65\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [134] "72\t3\t0.0\t4\t1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [135] "74\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [136] "75\t2\t0.0\t4\t1 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [137] "77\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [138] "79\t3\t0.0\t4\t1 1 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [139] "80\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [140] "81\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [141] "82\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [142] "83\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [143] "84\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [144] "86\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [145] "89\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [146] "90\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [147] "92\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [148] "94\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [149] "95\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [150] "96\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [151] "97\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [152] "99\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [153] "101\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [154] "102\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [155] "104\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [156] "105\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [157] "106\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [158] "108\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [159] "109\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [160] "111\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [161] "114\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [162] "115\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [163] "116\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [164] "117\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [165] "118\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [166] "119\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [167] "120\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [168] "121\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [169] "125\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [170] "131\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [171] "134\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [172] "136\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [173] "138\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [174] "139\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [175] "140\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [176] "141\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [177] "146\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [178] "147\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [179] "150\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [180] "158\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [181] "160\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [182] "177\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## 
## [[55]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/71A3_S55_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/71A3_S55_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/71A3_S55_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/71A3_S55_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##   [4] "Finished in 1.72 s (106 us/read; 0.57 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [8] "Total read pairs processed:             16,250"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [9] "  Read 1 with adapter:                      18 (0.1%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [10] "  Read 2 with adapter:                     120 (0.7%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [11] "Pairs written (passing filters):        16,249 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [13] "Total basepairs processed:     6,825,000 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [14] "  Read 1:     3,185,000 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [15] "  Read 2:     3,640,000 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [16] "Total written (filtered):      6,815,488 bp (99.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [17] "  Read 1:     3,183,427 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [18] "  Read 2:     3,632,061 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 3 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [29] "14\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [30] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [31] "21\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [32] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [34] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [36] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 15 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [38] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [39] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [40] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [41] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [42] "  A: 6.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [43] "  C: 40.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [44] "  G: 53.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [45] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [46] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [47] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [48] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [49] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [50] "52\t6\t0.0\t2\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [51] "61\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [52] "104\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [53] "124\t6\t0.0\t2\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [54] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [55] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [56] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [57] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [58] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [59] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [60] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [61] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [62] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 120 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [63] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [64] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [65] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [66] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [67] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [68] "  A: 50.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [69] "  C: 41.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [70] "  G: 5.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [71] "  T: 2.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [72] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [73] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [74] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [75] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [76] "5\t1\t15.9\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [77] "6\t1\t4.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [78] "7\t1\t1.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [79] "8\t3\t0.2\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [80] "9\t1\t0.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [81] "10\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [82] "11\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [83] "12\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [84] "13\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [85] "15\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [86] "16\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [87] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [88] "19\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [89] "20\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [90] "21\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [91] "22\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [92] "25\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [93] "28\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [94] "29\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [95] "31\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [96] "32\t4\t0.0\t3\t3 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [97] "33\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [98] "35\t3\t0.0\t3\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [99] "36\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [100] "38\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [101] "39\t5\t0.0\t3\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [102] "40\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [103] "41\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [104] "42\t2\t0.0\t4\t0 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [105] "43\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [106] "44\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [107] "47\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [108] "48\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [109] "51\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [110] "52\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [111] "54\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [112] "57\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [113] "59\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [114] "60\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [115] "62\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [116] "63\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [117] "65\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [118] "69\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [119] "70\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [120] "71\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [121] "72\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [122] "77\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [123] "78\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [124] "80\t7\t0.0\t4\t6 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [125] "82\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [126] "83\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [127] "88\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [128] "89\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [129] "92\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [130] "96\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [131] "97\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [132] "99\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [133] "100\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [134] "102\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [135] "103\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [136] "104\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [137] "105\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [138] "106\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [139] "108\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [140] "109\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [141] "110\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [142] "111\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [143] "117\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [144] "119\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [145] "120\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [146] "123\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [147] "129\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [148] "132\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [149] "138\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [150] "152\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [151] "160\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [152] "181\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## 
## [[56]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/71ARNA1_S56_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/71ARNA1_S56_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/71ARNA1_S56_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/71ARNA1_S56_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##   [4] "Finished in 0.79 s (111 us/read; 0.54 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [8] "Total read pairs processed:              7,101"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##   [9] "  Read 1 with adapter:                      20 (0.3%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [10] "  Read 2 with adapter:                     164 (2.3%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [11] "Pairs written (passing filters):         7,100 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [13] "Total basepairs processed:     2,982,420 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [14] "  Read 1:     1,391,796 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [15] "  Read 2:     1,590,624 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [16] "Total written (filtered):      2,969,324 bp (99.6%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [17] "  Read 1:     1,390,314 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [18] "  Read 2:     1,579,010 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 2 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [29] "6\t1\t1.7\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [30] "10\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [32] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [33] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [34] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [35] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 18 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [36] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [37] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [38] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [39] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [40] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [41] "  A: 11.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [42] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [43] "  G: 88.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [44] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [45] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [46] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [47] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [48] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [49] "10\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [50] "16\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [51] "24\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [52] "36\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [53] "59\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [54] "61\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [55] "73\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [56] "83\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [57] "90\t4\t0.0\t2\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [58] "142\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [59] "158\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [60] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [61] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [62] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [63] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [64] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [65] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [66] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [67] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [68] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [69] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [70] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [71] "5\t1\t6.9\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [72] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [73] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [74] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [75] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [76] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 163 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [77] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [78] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [79] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [80] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [81] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [82] "  A: 76.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [83] "  C: 17.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [84] "  G: 4.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [85] "  T: 0.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [86] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [87] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [88] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [89] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [90] "7\t2\t0.4\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [91] "8\t1\t0.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [92] "9\t3\t0.0\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [93] "10\t2\t0.0\t1\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [94] "12\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [95] "14\t2\t0.0\t1\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [96] "15\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [97] "16\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [98] "17\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [99] "18\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [100] "20\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [101] "21\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [102] "22\t3\t0.0\t2\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [103] "25\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [104] "27\t4\t0.0\t2\t3 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [105] "28\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [106] "29\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [107] "30\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [108] "32\t2\t0.0\t3\t0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [109] "34\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [110] "35\t3\t0.0\t3\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [111] "38\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [112] "39\t3\t0.0\t3\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [113] "40\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [114] "42\t3\t0.0\t4\t1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [115] "43\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [116] "44\t3\t0.0\t4\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [117] "45\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [118] "49\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [119] "51\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [120] "52\t5\t0.0\t4\t3 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [121] "53\t2\t0.0\t4\t0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [122] "54\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [123] "57\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [124] "58\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [125] "59\t3\t0.0\t4\t1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [126] "60\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [127] "61\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [128] "62\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [129] "63\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [130] "64\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [131] "65\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [132] "66\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [133] "69\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [134] "72\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [135] "73\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [136] "74\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [137] "76\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [138] "77\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [139] "79\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [140] "82\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [141] "84\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [142] "85\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [143] "86\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [144] "87\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [145] "89\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [146] "92\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [147] "93\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [148] "94\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [149] "95\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [150] "96\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [151] "97\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [152] "101\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [153] "102\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [154] "105\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [155] "106\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [156] "107\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [157] "110\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [158] "111\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [159] "112\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [160] "115\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [161] "116\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [162] "118\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [163] "120\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [164] "122\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [165] "125\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [166] "127\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [167] "131\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [168] "134\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [169] "135\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [170] "136\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [171] "140\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [172] "145\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [173] "146\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [174] "147\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [175] "165\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [176] "170\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [177] "186\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## 
## [[57]]
##  [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/71ARNA2_S57_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/71ARNA2_S57_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/71ARNA2_S57_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/71ARNA2_S57_L001_R2_001.fastq.gz"
##  [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [4] "Finished in 0.20 s (114 us/read; 0.53 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [8] "Total read pairs processed:              1,793"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [9] "  Read 1 with adapter:                       7 (0.4%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [10] "  Read 2 with adapter:                      33 (1.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [11] "Pairs written (passing filters):         1,793 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [13] "Total basepairs processed:       753,060 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [14] "  Read 1:       351,428 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [15] "  Read 2:       401,632 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [16] "Total written (filtered):        750,424 bp (99.6%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [17] "  Read 1:       350,991 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [18] "  Read 2:       399,433 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [24] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [25] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [26] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 7 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [27] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [28] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [29] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [30] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [31] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [32] "  A: 57.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [33] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [34] "  G: 42.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [35] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [36] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [38] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [39] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [40] "16\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [41] "63\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [42] "74\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [43] "84\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [44] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [45] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [46] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [47] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [48] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [49] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [50] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [51] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [52] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 33 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [53] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [54] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [55] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [56] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [57] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [58] "  A: 75.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [59] "  C: 21.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [60] "  G: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [61] "  T: 3.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [62] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [63] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [64] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [65] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [66] "9\t1\t0.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [67] "12\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [68] "13\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [69] "14\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [70] "18\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [71] "26\t1\t0.0\t2\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [72] "29\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [73] "39\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [74] "43\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [75] "44\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [76] "46\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [77] "49\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [78] "52\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [79] "54\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [80] "59\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [81] "63\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [82] "67\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [83] "79\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [84] "91\t3\t0.0\t4\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [85] "92\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [86] "98\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [87] "102\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [88] "112\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [89] "115\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [90] "116\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [91] "138\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [92] "165\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## 
## [[58]]
##  [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/71ARNA3_S58_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/71ARNA3_S58_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/71ARNA3_S58_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/71ARNA3_S58_L001_R2_001.fastq.gz"
##  [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [4] "Finished in 0.04 s (171 us/read; 0.35 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [8] "Total read pairs processed:                209"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [9] "  Read 1 with adapter:                       2 (1.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [10] "  Read 2 with adapter:                       9 (4.3%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [11] "Pairs written (passing filters):           208 (99.5%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [13] "Total basepairs processed:        87,780 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [14] "  Read 1:        40,964 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [15] "  Read 2:        46,816 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [16] "Total written (filtered):         86,475 bp (98.5%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [17] "  Read 1:        40,628 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [18] "  Read 2:        45,847 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 2 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [29] "36\t1\t0.0\t3\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [30] "73\t1\t0.0\t4\t0 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [32] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [33] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [34] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [35] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [36] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [37] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [38] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [39] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [40] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [41] "  A: 100.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [42] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [43] "  G: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [44] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [45] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [46] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [47] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [48] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [49] "140\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [50] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [51] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [52] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [53] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [54] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [55] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [56] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [57] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [58] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 10 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [59] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [60] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [61] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [62] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [63] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [64] "  A: 70.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [65] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [66] "  G: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [67] "  T: 30.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [68] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [69] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [70] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [71] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [72] "59\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [73] "60\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [74] "72\t1\t0.0\t4\t0 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [75] "73\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [76] "80\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [77] "85\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [78] "121\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [79] "131\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [80] "136\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [81] "138\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## 
## [[59]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/71B1_S59_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/71B1_S59_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/71B1_S59_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/71B1_S59_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##   [4] "Finished in 1.82 s (110 us/read; 0.55 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [8] "Total read pairs processed:             16,557"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [9] "  Read 1 with adapter:                       1 (0.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [10] "  Read 2 with adapter:                      98 (0.6%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [11] "Pairs written (passing filters):        16,557 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [13] "Total basepairs processed:     6,953,940 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [14] "  Read 1:     3,245,172 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [15] "  Read 2:     3,708,768 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [16] "Total written (filtered):      6,948,082 bp (99.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [17] "  Read 1:     3,245,167 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [18] "  Read 2:     3,702,915 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [29] "5\t1\t16.2\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [30] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [32] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [34] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [36] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [38] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [39] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [40] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [41] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [42] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 99 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [43] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [44] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [45] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [46] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [47] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [48] "  A: 57.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [49] "  C: 40.4%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [50] "  G: 1.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [51] "  T: 1.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [52] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [53] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [54] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [55] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [56] "5\t3\t16.2\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [57] "7\t2\t1.0\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [58] "10\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [59] "11\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [60] "12\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [61] "13\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [62] "15\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [63] "16\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [64] "20\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [65] "22\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [66] "26\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [67] "27\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [68] "28\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [69] "29\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [70] "30\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [71] "31\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [72] "32\t3\t0.0\t3\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [73] "33\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [74] "34\t4\t0.0\t3\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [75] "35\t1\t0.0\t3\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [76] "38\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [77] "39\t4\t0.0\t3\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [78] "40\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [79] "43\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [80] "45\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [81] "46\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [82] "47\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [83] "48\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [84] "52\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [85] "59\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [86] "61\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [87] "63\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [88] "64\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [89] "66\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [90] "67\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [91] "72\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [92] "73\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [93] "74\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [94] "75\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [95] "79\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [96] "82\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [97] "83\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [98] "84\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [99] "85\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [100] "86\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [101] "87\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [102] "88\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [103] "93\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [104] "96\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [105] "99\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [106] "103\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [107] "109\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [108] "111\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [109] "112\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [110] "122\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [111] "126\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [112] "127\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [113] "136\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [114] "142\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [115] "144\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## 
## [[60]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/71BRNA4_S60_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/71BRNA4_S60_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/71BRNA4_S60_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/71BRNA4_S60_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##   [4] "Finished in 0.14 s (125 us/read; 0.48 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [8] "Total read pairs processed:              1,149"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##   [9] "  Read 1 with adapter:                      30 (2.6%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [10] "  Read 2 with adapter:                      46 (4.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [11] "Pairs written (passing filters):         1,148 (99.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [13] "Total basepairs processed:       482,580 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [14] "  Read 1:       225,204 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [15] "  Read 2:       257,376 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [16] "Total written (filtered):        476,004 bp (98.6%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [17] "  Read 1:       223,017 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [18] "  Read 2:       252,987 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [29] "45\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [30] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [32] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [34] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 29 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [36] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [37] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [39] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [40] "  A: 34.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [41] "  C: 13.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [42] "  G: 51.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [43] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [44] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [45] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [46] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [47] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [48] "8\t1\t0.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [49] "14\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [50] "21\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [51] "23\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [52] "52\t4\t0.0\t2\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [53] "61\t4\t0.0\t2\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [54] "62\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [55] "75\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [56] "82\t5\t0.0\t2\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [57] "90\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [58] "91\t4\t0.0\t2\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [59] "93\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [60] "118\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [61] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [62] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [63] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [64] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [65] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [66] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [67] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [68] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [69] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 46 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [70] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [71] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [72] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [73] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [74] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [75] "  A: 84.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [76] "  C: 10.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [77] "  G: 4.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [78] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [79] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [80] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [81] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [82] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [83] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [84] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [85] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [86] "5\t1\t1.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [87] "35\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [88] "36\t1\t0.0\t3\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [89] "41\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [90] "42\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [91] "48\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [92] "49\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [93] "51\t2\t0.0\t4\t0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [94] "66\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [95] "74\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [96] "80\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [97] "83\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [98] "89\t4\t0.0\t4\t0 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [99] "90\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [100] "94\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [101] "103\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [102] "107\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [103] "109\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [104] "110\t5\t0.0\t4\t3 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [105] "112\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [106] "117\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [107] "118\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [108] "119\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [109] "121\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [110] "128\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [111] "133\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [112] "136\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [113] "146\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [114] "183\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [115] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [116] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [117] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [118] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [119] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## 
## [[61]]
##  [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/BA11_S61_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/BA11_S61_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/BA11_S61_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/BA11_S61_L001_R2_001.fastq.gz"
##  [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [4] "Finished in 0.04 s (138 us/read; 0.44 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [8] "Total read pairs processed:                281"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [9] "  Read 1 with adapter:                       0 (0.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [10] "  Read 2 with adapter:                       5 (1.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [11] "Pairs written (passing filters):           281 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [13] "Total basepairs processed:       118,020 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [14] "  Read 1:        55,076 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [15] "  Read 2:        62,944 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [16] "Total written (filtered):        117,669 bp (99.7%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [17] "  Read 1:        55,076 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [18] "  Read 2:        62,593 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [24] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [25] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [26] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [27] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [28] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [29] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [30] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [32] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [34] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 5 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [36] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [37] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [39] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [40] "  A: 80.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [41] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [42] "  G: 20.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [43] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [44] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [45] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [46] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [47] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [48] "30\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [49] "53\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [50] "76\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [51] "94\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [52] "98\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## 
## [[62]]
##  [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/BA12_S62_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/BA12_S62_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/BA12_S62_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/BA12_S62_L001_R2_001.fastq.gz"
##  [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [4] "Finished in 0.09 s (119 us/read; 0.51 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [8] "Total read pairs processed:                793"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [9] "  Read 1 with adapter:                       4 (0.5%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [10] "  Read 2 with adapter:                      12 (1.5%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [11] "Pairs written (passing filters):           793 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [13] "Total basepairs processed:       333,060 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [14] "  Read 1:       155,428 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [15] "  Read 2:       177,632 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [16] "Total written (filtered):        331,359 bp (99.5%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [17] "  Read 1:       154,886 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [18] "  Read 2:       176,473 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [24] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [25] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [26] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 4 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [27] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [28] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [29] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [30] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [31] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [32] "  A: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [33] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [34] "  G: 100.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [35] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [36] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [38] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [39] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [40] "104\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [41] "146\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [42] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [43] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [44] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [45] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [46] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [47] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [48] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [49] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [50] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 12 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [51] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [52] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [53] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [54] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [55] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [56] "  A: 50.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [57] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [58] "  G: 41.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [59] "  T: 8.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [60] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [61] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [62] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [63] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [64] "7\t1\t0.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [65] "8\t1\t0.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [66] "51\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [67] "56\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [68] "67\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [69] "72\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [70] "106\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [71] "132\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [72] "138\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [73] "174\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## 
## [[63]]
##  [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/BA13_S63_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/BA13_S63_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/BA13_S63_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/BA13_S63_L001_R2_001.fastq.gz"
##  [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [4] "Finished in 0.21 s (112 us/read; 0.53 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [8] "Total read pairs processed:              1,909"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [9] "  Read 1 with adapter:                       0 (0.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [10] "  Read 2 with adapter:                      12 (0.6%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [11] "Pairs written (passing filters):         1,908 (99.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [13] "Total basepairs processed:       801,780 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [14] "  Read 1:       374,164 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [15] "  Read 2:       427,616 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [16] "Total written (filtered):        800,835 bp (99.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [17] "  Read 1:       373,968 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [18] "  Read 2:       426,867 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [24] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [25] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [26] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [27] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [28] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [29] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [30] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [32] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [34] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 12 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [36] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [37] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [39] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [40] "  A: 75.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [41] "  C: 16.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [42] "  G: 8.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [43] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [44] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [45] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [46] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [47] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [48] "5\t2\t1.9\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [49] "14\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [50] "16\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [51] "42\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [52] "57\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [53] "68\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [54] "73\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [55] "82\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [56] "106\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [57] "179\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## 
## [[64]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/BA1DF1_S66_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/BA1DF1_S66_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/BA1DF1_S66_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/BA1DF1_S66_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##   [4] "Finished in 2.49 s (102 us/read; 0.59 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##   [8] "Total read pairs processed:             24,379"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [9] "  Read 1 with adapter:                       5 (0.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [10] "  Read 2 with adapter:                     428 (1.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [11] "Pairs written (passing filters):        24,379 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [13] "Total basepairs processed:    10,239,180 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [14] "  Read 1:     4,778,284 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [15] "  Read 2:     5,460,896 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [16] "Total written (filtered):     10,209,110 bp (99.7%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [17] "  Read 1:     4,778,181 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [18] "  Read 2:     5,430,929 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 4 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [29] "5\t1\t23.8\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [30] "8\t1\t0.4\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [31] "22\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [32] "30\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [34] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [35] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [36] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [37] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [39] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [40] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [41] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [42] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [43] "  A: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [44] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [45] "  G: 100.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [46] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [47] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [48] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [49] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [50] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [51] "38\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [52] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [53] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [54] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [55] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [56] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 2 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [57] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [58] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [59] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [60] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [61] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [62] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [63] "7\t1\t1.5\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [64] "9\t1\t0.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [65] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [66] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [67] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [68] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [69] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 426 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [70] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [71] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [72] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [73] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [74] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [75] "  A: 91.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [76] "  C: 7.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [77] "  G: 0.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [78] "  T: 0.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [79] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [80] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [81] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [82] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [83] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [84] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [85] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [86] "5\t32\t23.8\t0\t32"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [87] "6\t1\t6.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [88] "7\t2\t1.5\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [89] "8\t9\t0.4\t0\t9"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [90] "9\t4\t0.1\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [91] "10\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [92] "12\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [93] "13\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [94] "14\t12\t0.0\t1\t12"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [95] "15\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [96] "16\t5\t0.0\t1\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [97] "17\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [98] "18\t3\t0.0\t1\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [99] "19\t3\t0.0\t1\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [100] "20\t3\t0.0\t2\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [101] "21\t3\t0.0\t2\t1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [102] "22\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [103] "23\t4\t0.0\t2\t2 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [104] "24\t2\t0.0\t2\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [105] "25\t2\t0.0\t2\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [106] "26\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [107] "27\t3\t0.0\t2\t1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [108] "29\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [109] "30\t2\t0.0\t3\t1 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [110] "31\t1\t0.0\t3\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [111] "32\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [112] "33\t3\t0.0\t3\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [113] "34\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [114] "35\t3\t0.0\t3\t1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [115] "36\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [116] "38\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [117] "41\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [118] "42\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [119] "44\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [120] "45\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [121] "46\t11\t0.0\t4\t7 2 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [122] "47\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [123] "48\t3\t0.0\t4\t1 1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [124] "51\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [125] "52\t9\t0.0\t4\t8 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [126] "53\t3\t0.0\t4\t0 1 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [127] "54\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [128] "55\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [129] "56\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [130] "57\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [131] "58\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [132] "60\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [133] "61\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [134] "62\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [135] "63\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [136] "64\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [137] "65\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [138] "66\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [139] "67\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [140] "70\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [141] "71\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [142] "72\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [143] "74\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [144] "75\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [145] "76\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [146] "77\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [147] "79\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [148] "80\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [149] "82\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [150] "83\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [151] "84\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [152] "85\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [153] "86\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [154] "87\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [155] "88\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [156] "89\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [157] "91\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [158] "92\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [159] "93\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [160] "94\t5\t0.0\t4\t3 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [161] "95\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [162] "96\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [163] "97\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [164] "98\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [165] "99\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [166] "100\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [167] "102\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [168] "104\t18\t0.0\t4\t16 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [169] "105\t96\t0.0\t4\t86 10"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [170] "106\t7\t0.0\t4\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [171] "107\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [172] "108\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [173] "110\t4\t0.0\t4\t2 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [174] "112\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [175] "113\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [176] "114\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [177] "115\t4\t0.0\t4\t3 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [178] "116\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [179] "118\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [180] "119\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [181] "121\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [182] "123\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [183] "124\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [184] "127\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [185] "128\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [186] "131\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [187] "132\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [188] "135\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [189] "146\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [190] "147\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [191] "149\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [192] "153\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [193] "164\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [194] "165\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [195] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [196] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [197] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [198] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [199] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## 
## [[65]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/BA1DF2_S67_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/BA1DF2_S67_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/BA1DF2_S67_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/BA1DF2_S67_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##   [4] "Finished in 1.14 s (103 us/read; 0.58 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##   [8] "Total read pairs processed:             11,111"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [9] "  Read 1 with adapter:                       3 (0.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [10] "  Read 2 with adapter:                     129 (1.2%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [11] "Pairs written (passing filters):        11,111 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [13] "Total basepairs processed:     4,666,620 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [14] "  Read 1:     2,177,756 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [15] "  Read 2:     2,488,864 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [16] "Total written (filtered):      4,658,068 bp (99.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [17] "  Read 1:     2,177,659 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [18] "  Read 2:     2,480,409 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 2 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [29] "5\t1\t10.9\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [30] "33\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [32] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [33] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [34] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [35] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [36] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [37] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [38] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [39] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [40] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [41] "  A: 100.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [42] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [43] "  G: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [44] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [45] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [46] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [47] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [48] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [49] "59\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [50] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [51] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [52] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [53] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [54] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [55] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [56] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [57] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [58] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [59] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [60] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [61] "8\t1\t0.2\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [62] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [63] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [64] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [65] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [66] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 128 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [67] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [68] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [69] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [70] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [71] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [72] "  A: 93.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [73] "  C: 7.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [74] "  G: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [75] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [76] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [77] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [78] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [79] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [80] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [81] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [82] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [83] "5\t9\t10.9\t0\t9"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [84] "9\t1\t0.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [85] "11\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [86] "12\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [87] "14\t4\t0.0\t1\t2 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [88] "15\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [89] "17\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [90] "18\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [91] "20\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [92] "21\t2\t0.0\t2\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [93] "22\t2\t0.0\t2\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [94] "24\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [95] "26\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [96] "27\t3\t0.0\t2\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [97] "28\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [98] "29\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [99] "30\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [100] "31\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [101] "33\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [102] "34\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [103] "35\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [104] "38\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [105] "39\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [106] "40\t3\t0.0\t4\t1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [107] "43\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [108] "45\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [109] "46\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [110] "47\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [111] "48\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [112] "51\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [113] "52\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [114] "53\t1\t0.0\t4\t0 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [115] "55\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [116] "57\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [117] "59\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [118] "60\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [119] "61\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [120] "62\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [121] "63\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [122] "70\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [123] "71\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [124] "72\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [125] "73\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [126] "77\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [127] "80\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [128] "82\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [129] "85\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [130] "86\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [131] "87\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [132] "88\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [133] "97\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [134] "100\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [135] "103\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [136] "104\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [137] "105\t26\t0.0\t4\t24 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [138] "106\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [139] "107\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [140] "110\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [141] "112\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [142] "119\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [143] "128\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [144] "131\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [145] "133\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [146] "137\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [147] "147\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [148] "149\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [149] "153\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [150] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [151] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [152] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [153] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [154] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## 
## [[66]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/BA1DFRNA1_S64_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/BA1DFRNA1_S64_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/BA1DFRNA1_S64_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/BA1DFRNA1_S64_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##   [4] "Finished in 1.71 s (101 us/read; 0.60 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##   [8] "Total read pairs processed:             17,045"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##   [9] "  Read 1 with adapter:                      71 (0.4%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [10] "  Read 2 with adapter:                     684 (4.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [11] "Pairs written (passing filters):        17,044 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [13] "Total basepairs processed:     7,158,900 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [14] "  Read 1:     3,340,820 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [15] "  Read 2:     3,818,080 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [16] "Total written (filtered):      7,104,091 bp (99.2%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [17] "  Read 1:     3,337,791 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [18] "  Read 2:     3,766,300 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [29] "66\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [30] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [32] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [34] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 70 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [36] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [37] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [39] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [40] "  A: 65.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [41] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [42] "  G: 34.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [43] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [44] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [45] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [46] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [47] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [48] "5\t1\t16.6\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [49] "6\t4\t4.2\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [50] "7\t1\t1.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [51] "8\t1\t0.3\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [52] "9\t3\t0.1\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [53] "10\t2\t0.0\t1\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [54] "11\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [55] "16\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [56] "18\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [57] "19\t7\t0.0\t1\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [58] "20\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [59] "22\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [60] "23\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [61] "27\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [62] "28\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [63] "30\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [64] "32\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [65] "33\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [66] "36\t7\t0.0\t2\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [67] "38\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [68] "39\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [69] "41\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [70] "42\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [71] "43\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [72] "44\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [73] "45\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [74] "46\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [75] "48\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [76] "49\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [77] "60\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [78] "64\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [79] "68\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [80] "69\t6\t0.0\t2\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [81] "70\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [82] "71\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [83] "75\t4\t0.0\t2\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [84] "83\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [85] "84\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [86] "109\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [87] "130\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [88] "150\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [89] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [90] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [91] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [92] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [93] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [94] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [95] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [96] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [97] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 684 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [98] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [99] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [100] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [101] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [102] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [103] "  A: 97.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [104] "  C: 2.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [105] "  G: 0.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [106] "  T: 0.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [107] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [108] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [109] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [110] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [111] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [112] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [113] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [114] "5\t4\t16.6\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [115] "6\t1\t4.2\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [116] "7\t2\t1.0\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [117] "8\t6\t0.3\t0\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [118] "9\t10\t0.1\t0\t10"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [119] "10\t6\t0.0\t1\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [120] "11\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [121] "12\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [122] "13\t5\t0.0\t1\t3 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [123] "14\t5\t0.0\t1\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [124] "15\t2\t0.0\t1\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [125] "16\t4\t0.0\t1\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [126] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [127] "18\t4\t0.0\t1\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [128] "20\t2\t0.0\t2\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [129] "21\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [130] "22\t5\t0.0\t2\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [131] "23\t6\t0.0\t2\t4 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [132] "24\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [133] "25\t5\t0.0\t2\t4 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [134] "26\t4\t0.0\t2\t1 2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [135] "27\t24\t0.0\t2\t20 2 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [136] "28\t4\t0.0\t2\t3 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [137] "29\t3\t0.0\t2\t1 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [138] "30\t4\t0.0\t3\t3 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [139] "31\t3\t0.0\t3\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [140] "32\t6\t0.0\t3\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [141] "33\t5\t0.0\t3\t3 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [142] "34\t10\t0.0\t3\t7 2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [143] "35\t3\t0.0\t3\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [144] "36\t6\t0.0\t3\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [145] "37\t5\t0.0\t3\t3 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [146] "38\t3\t0.0\t3\t1 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [147] "39\t3\t0.0\t3\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [148] "40\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [149] "41\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [150] "42\t3\t0.0\t4\t0 2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [151] "43\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [152] "44\t4\t0.0\t4\t2 0 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [153] "45\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [154] "46\t8\t0.0\t4\t6 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [155] "47\t13\t0.0\t4\t8 4 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [156] "48\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [157] "50\t3\t0.0\t4\t2 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [158] "51\t12\t0.0\t4\t11 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [159] "52\t19\t0.0\t4\t15 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [160] "53\t7\t0.0\t4\t6 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [161] "54\t7\t0.0\t4\t4 2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [162] "55\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [163] "56\t3\t0.0\t4\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [164] "57\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [165] "58\t11\t0.0\t4\t9 0 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [166] "60\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [167] "61\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [168] "62\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [169] "64\t11\t0.0\t4\t8 2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [170] "65\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [171] "66\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [172] "67\t9\t0.0\t4\t8 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [173] "69\t3\t0.0\t4\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [174] "70\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [175] "71\t3\t0.0\t4\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [176] "72\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [177] "73\t5\t0.0\t4\t3 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [178] "74\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [179] "75\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [180] "76\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [181] "77\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [182] "78\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [183] "79\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [184] "80\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [185] "81\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [186] "83\t2\t0.0\t4\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [187] "84\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [188] "85\t3\t0.0\t4\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [189] "86\t14\t0.0\t4\t13 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [190] "87\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [191] "88\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [192] "89\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [193] "90\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [194] "91\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [195] "92\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [196] "94\t9\t0.0\t4\t8 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [197] "95\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [198] "96\t6\t0.0\t4\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [199] "97\t11\t0.0\t4\t9 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [200] "98\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [201] "99\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [202] "100\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [203] "101\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [204] "103\t11\t0.0\t4\t10 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [205] "104\t43\t0.0\t4\t41 1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [206] "105\t85\t0.0\t4\t76 8 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [207] "106\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [208] "108\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [209] "109\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [210] "110\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [211] "111\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [212] "112\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [213] "113\t7\t0.0\t4\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [214] "114\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [215] "115\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [216] "116\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [217] "117\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [218] "118\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [219] "119\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [220] "120\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [221] "121\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [222] "122\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [223] "123\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [224] "124\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [225] "125\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [226] "126\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [227] "127\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [228] "128\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [229] "129\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [230] "130\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [231] "131\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [232] "132\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [233] "133\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [234] "135\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [235] "136\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [236] "137\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [237] "139\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [238] "140\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [239] "141\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [240] "142\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [241] "144\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [242] "145\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [243] "146\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [244] "149\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [245] "151\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [246] "153\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [247] "154\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [248] "158\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [249] "161\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [250] "162\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [251] "165\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [252] "167\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [253] "171\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [254] "178\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [255] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [256] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [257] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [258] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [259] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## 
## [[67]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/BA1DFRNA2_S65_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/BA1DFRNA2_S65_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/BA1DFRNA2_S65_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/BA1DFRNA2_S65_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##   [4] "Finished in 0.92 s (101 us/read; 0.59 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##   [8] "Total read pairs processed:              9,062"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##   [9] "  Read 1 with adapter:                      89 (1.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [10] "  Read 2 with adapter:                     348 (3.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [11] "Pairs written (passing filters):         9,062 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [13] "Total basepairs processed:     3,806,040 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [14] "  Read 1:     1,776,152 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [15] "  Read 2:     2,029,888 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [16] "Total written (filtered):      3,776,646 bp (99.2%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [17] "  Read 1:     1,771,398 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [18] "  Read 2:     2,005,248 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 6 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [29] "5\t1\t8.8\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [30] "6\t1\t2.2\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [31] "7\t1\t0.6\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [32] "10\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [33] "16\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [34] "32\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [36] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [37] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [39] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 83 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [40] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [41] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [42] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [43] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [44] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [45] "  A: 67.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [46] "  C: 1.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [47] "  G: 30.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [48] "  T: 1.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [49] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [50] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [51] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [52] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [53] "6\t1\t2.2\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [54] "7\t1\t0.6\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [55] "8\t2\t0.1\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [56] "10\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [57] "15\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [58] "16\t4\t0.0\t1\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [59] "18\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [60] "19\t4\t0.0\t1\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [61] "20\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [62] "25\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [63] "27\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [64] "31\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [65] "32\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [66] "33\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [67] "35\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [68] "36\t10\t0.0\t2\t10"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [69] "39\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [70] "40\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [71] "41\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [72] "44\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [73] "50\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [74] "55\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [75] "57\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [76] "65\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [77] "68\t12\t0.0\t2\t12"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [78] "69\t4\t0.0\t2\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [79] "72\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [80] "74\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [81] "75\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [82] "77\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [83] "78\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [84] "79\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [85] "82\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [86] "86\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [87] "91\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [88] "92\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [89] "98\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [90] "99\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [91] "101\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [92] "105\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [93] "111\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [94] "120\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [95] "126\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [96] "137\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [97] "144\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [98] "146\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [99] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [100] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [101] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [102] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [103] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 2 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [104] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [105] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [106] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [107] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [108] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [109] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [110] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [111] "125\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [112] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [113] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [114] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [115] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [116] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 346 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [117] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [118] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [119] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [120] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [121] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [122] "  A: 96.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [123] "  C: 2.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [124] "  G: 0.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [125] "  T: 1.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [126] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [127] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [128] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [129] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [130] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [131] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [132] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [133] "5\t7\t8.8\t0\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [134] "7\t2\t0.6\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [135] "9\t4\t0.0\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [136] "10\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [137] "11\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [138] "12\t2\t0.0\t1\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [139] "13\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [140] "14\t2\t0.0\t1\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [141] "16\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [142] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [143] "19\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [144] "20\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [145] "21\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [146] "22\t4\t0.0\t2\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [147] "25\t8\t0.0\t2\t7 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [148] "26\t2\t0.0\t2\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [149] "27\t21\t0.0\t2\t17 3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [150] "28\t2\t0.0\t2\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [151] "29\t3\t0.0\t2\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [152] "30\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [153] "31\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [154] "32\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [155] "33\t3\t0.0\t3\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [156] "34\t4\t0.0\t3\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [157] "35\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [158] "36\t3\t0.0\t3\t1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [159] "37\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [160] "38\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [161] "39\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [162] "40\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [163] "42\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [164] "43\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [165] "44\t4\t0.0\t4\t0 1 1 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [166] "46\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [167] "47\t7\t0.0\t4\t4 0 2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [168] "48\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [169] "49\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [170] "50\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [171] "51\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [172] "52\t6\t0.0\t4\t3 1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [173] "53\t4\t0.0\t4\t3 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [174] "55\t3\t0.0\t4\t1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [175] "56\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [176] "58\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [177] "59\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [178] "60\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [179] "61\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [180] "63\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [181] "64\t15\t0.0\t4\t11 3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [182] "65\t4\t0.0\t4\t2 1 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [183] "66\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [184] "67\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [185] "68\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [186] "69\t2\t0.0\t4\t1 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [187] "70\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [188] "71\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [189] "72\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [190] "73\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [191] "74\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [192] "75\t6\t0.0\t4\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [193] "76\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [194] "77\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [195] "78\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [196] "79\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [197] "80\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [198] "83\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [199] "84\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [200] "85\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [201] "86\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [202] "87\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [203] "89\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [204] "91\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [205] "92\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [206] "93\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [207] "94\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [208] "95\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [209] "96\t13\t0.0\t4\t13"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [210] "97\t8\t0.0\t4\t8"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [211] "99\t3\t0.0\t4\t1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [212] "101\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [213] "102\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [214] "103\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [215] "104\t8\t0.0\t4\t8"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [216] "105\t22\t0.0\t4\t18 3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [217] "106\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [218] "107\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [219] "108\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [220] "110\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [221] "111\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [222] "113\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [223] "114\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [224] "115\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [225] "118\t1\t0.0\t4\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [226] "119\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [227] "120\t3\t0.0\t4\t1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [228] "121\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [229] "122\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [230] "124\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [231] "126\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [232] "127\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [233] "128\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [234] "129\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [235] "133\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [236] "135\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [237] "136\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [238] "138\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [239] "139\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [240] "140\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [241] "146\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [242] "148\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [243] "152\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [244] "154\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [245] "162\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [246] "165\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [247] "172\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [248] "174\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [249] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [250] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [251] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [252] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [253] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## 
## [[68]]
##  [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/BA1RNA1_S68_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/BA1RNA1_S68_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/BA1RNA1_S68_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/BA1RNA1_S68_L001_R2_001.fastq.gz"
##  [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [4] "Finished in 0.03 s (157 us/read; 0.38 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [8] "Total read pairs processed:                189"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [9] "  Read 1 with adapter:                       0 (0.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [10] "  Read 2 with adapter:                       6 (3.2%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [11] "Pairs written (passing filters):           189 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [13] "Total basepairs processed:        79,380 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [14] "  Read 1:        37,044 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [15] "  Read 2:        42,336 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [16] "Total written (filtered):         79,007 bp (99.5%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [17] "  Read 1:        37,044 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [18] "  Read 2:        41,963 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [24] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [25] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [26] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [27] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [28] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [29] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [30] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [32] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [34] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 6 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [36] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [37] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [39] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [40] "  A: 83.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [41] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [42] "  G: 16.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [43] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [44] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [45] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [46] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [47] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [48] "16\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [49] "34\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [50] "66\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [51] "69\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [52] "85\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [53] "103\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## 
## [[69]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/BA1RNA2_S69_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/BA1RNA2_S69_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/BA1RNA2_S69_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/BA1RNA2_S69_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##   [4] "Finished in 0.08 s (137 us/read; 0.44 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [8] "Total read pairs processed:                603"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##   [9] "  Read 1 with adapter:                      16 (2.7%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [10] "  Read 2 with adapter:                      37 (6.1%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [11] "Pairs written (passing filters):           603 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [13] "Total basepairs processed:       253,260 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [14] "  Read 1:       118,188 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [15] "  Read 2:       135,072 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [16] "Total written (filtered):        248,388 bp (98.1%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [17] "  Read 1:       116,641 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [18] "  Read 2:       131,747 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [24] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [25] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [26] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 16 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [27] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [28] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [29] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [30] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [31] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [32] "  A: 25.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [33] "  C: 37.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [34] "  G: 25.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [35] "  T: 12.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [36] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [38] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [39] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [40] "18\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [41] "74\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [42] "75\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [43] "82\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [44] "96\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [45] "119\t4\t0.0\t2\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [46] "138\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [47] "141\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [48] "142\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [49] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [50] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [51] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [52] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [53] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [54] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [55] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [56] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [57] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 37 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [58] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [59] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [60] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [61] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [62] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [63] "  A: 91.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [64] "  C: 2.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [65] "  G: 5.4%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [66] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [67] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [68] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [69] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [70] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [71] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [72] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [73] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [74] "5\t1\t0.6\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [75] "13\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [76] "16\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [77] "28\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [78] "29\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [79] "44\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [80] "45\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [81] "50\t2\t0.0\t4\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [82] "55\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [83] "66\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [84] "67\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [85] "70\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [86] "75\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [87] "80\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [88] "83\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [89] "86\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [90] "98\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [91] "102\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [92] "103\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [93] "104\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [94] "107\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [95] "108\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [96] "110\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [97] "116\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [98] "131\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [99] "143\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [100] "147\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [101] "166\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [102] "170\t1\t0.0\t4\t0 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [103] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [104] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [105] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [106] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [107] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## 
## [[70]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/BA1RNA3_S70_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/BA1RNA3_S70_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/BA1RNA3_S70_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/BA1RNA3_S70_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##   [4] "Finished in 0.27 s (119 us/read; 0.50 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [8] "Total read pairs processed:              2,235"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##   [9] "  Read 1 with adapter:                       8 (0.4%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [10] "  Read 2 with adapter:                      38 (1.7%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [11] "Pairs written (passing filters):         2,235 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [13] "Total basepairs processed:       938,700 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [14] "  Read 1:       438,060 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [15] "  Read 2:       500,640 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [16] "Total written (filtered):        935,856 bp (99.7%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [17] "  Read 1:       437,753 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [18] "  Read 2:       498,103 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 4 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [29] "16\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [30] "30\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [31] "58\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [32] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [34] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [36] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 4 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [38] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [39] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [40] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [41] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [42] "  A: 25.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [43] "  C: 25.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [44] "  G: 50.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [45] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [46] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [47] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [48] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [49] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [50] "19\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [51] "36\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [52] "82\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [53] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [54] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [55] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [56] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [57] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [58] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [59] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [60] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [61] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [62] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [63] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [64] "6\t1\t0.5\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [65] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [66] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [67] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [68] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [69] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 38 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [70] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [71] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [72] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [73] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [74] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [75] "  A: 86.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [76] "  C: 7.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [77] "  G: 5.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [78] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [79] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [80] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [81] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [82] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [83] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [84] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [85] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [86] "7\t1\t0.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [87] "11\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [88] "13\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [89] "16\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [90] "20\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [91] "23\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [92] "27\t1\t0.0\t2\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [93] "29\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [94] "33\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [95] "34\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [96] "35\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [97] "43\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [98] "47\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [99] "51\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [100] "52\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [101] "53\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [102] "59\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [103] "61\t1\t0.0\t4\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [104] "64\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [105] "74\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [106] "82\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [107] "86\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [108] "91\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [109] "93\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [110] "94\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [111] "98\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [112] "101\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [113] "105\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [114] "110\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [115] "145\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [116] "146\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [117] "147\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [118] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [119] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [120] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [121] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [122] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## 
## [[71]]
##  [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/BT1B1_S71_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/BT1B1_S71_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/BT1B1_S71_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/BT1B1_S71_L001_R2_001.fastq.gz"
##  [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [4] "Finished in 0.09 s (126 us/read; 0.48 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [8] "Total read pairs processed:                751"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [9] "  Read 1 with adapter:                       3 (0.4%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [10] "  Read 2 with adapter:                      12 (1.6%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [11] "Pairs written (passing filters):           751 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [13] "Total basepairs processed:       315,420 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [14] "  Read 1:       147,196 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [15] "  Read 2:       168,224 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [16] "Total written (filtered):        314,066 bp (99.6%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [17] "  Read 1:       146,913 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [18] "  Read 2:       167,153 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [29] "5\t1\t0.7\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [30] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [32] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [34] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 2 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [36] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [37] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [39] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [40] "  A: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [41] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [42] "  G: 100.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [43] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [44] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [45] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [46] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [47] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [48] "137\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [49] "141\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [50] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [51] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [52] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [53] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [54] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [55] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [56] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [57] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [58] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [59] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [60] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [61] "5\t1\t0.7\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [62] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [63] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [64] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [65] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [66] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 12 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [67] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [68] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [69] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [70] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [71] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [72] "  A: 91.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [73] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [74] "  G: 8.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [75] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [76] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [77] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [78] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [79] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [80] "19\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [81] "50\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [82] "59\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [83] "63\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [84] "79\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [85] "91\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [86] "100\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [87] "108\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [88] "112\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [89] "113\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [90] "131\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [91] "141\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## 
## [[72]]
##  [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/BT1B2_S72_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/BT1B2_S72_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/BT1B2_S72_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/BT1B2_S72_L001_R2_001.fastq.gz"
##  [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [4] "Finished in 0.03 s (185 us/read; 0.32 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [8] "Total read pairs processed:                148"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [9] "  Read 1 with adapter:                       3 (2.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [10] "  Read 2 with adapter:                       5 (3.4%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [11] "Pairs written (passing filters):           148 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [13] "Total basepairs processed:        62,160 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [14] "  Read 1:        29,008 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [15] "  Read 2:        33,152 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [16] "Total written (filtered):         61,570 bp (99.1%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [17] "  Read 1:        28,759 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [18] "  Read 2:        32,811 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [24] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [25] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [26] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 3 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [27] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [28] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [29] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [30] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [31] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [32] "  A: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [33] "  C: 33.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [34] "  G: 66.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [35] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [36] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [38] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [39] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [40] "5\t1\t0.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [41] "107\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [42] "137\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [43] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [44] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [45] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [46] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [47] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [48] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [49] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [50] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [51] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 5 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [52] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [53] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [54] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [55] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [56] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [57] "  A: 40.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [58] "  C: 60.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [59] "  G: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [60] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [61] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [62] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [63] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [64] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [65] "22\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [66] "32\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [67] "65\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [68] "87\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [69] "135\t1\t0.0\t4\t0 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## 
## [[73]]
##  [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/BT1B3_S73_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/BT1B3_S73_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/BT1B3_S73_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/BT1B3_S73_L001_R2_001.fastq.gz"
##  [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [4] "Finished in 0.03 s (193 us/read; 0.31 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [8] "Total read pairs processed:                132"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [9] "  Read 1 with adapter:                       0 (0.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [10] "  Read 2 with adapter:                      10 (7.6%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [11] "Pairs written (passing filters):           132 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [13] "Total basepairs processed:        55,440 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [14] "  Read 1:        25,872 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [15] "  Read 2:        29,568 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [16] "Total written (filtered):         54,633 bp (98.5%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [17] "  Read 1:        25,872 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [18] "  Read 2:        28,761 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [24] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [25] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [26] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [27] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [28] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [29] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [30] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [32] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [34] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 10 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [36] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [37] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [39] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [40] "  A: 70.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [41] "  C: 10.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [42] "  G: 20.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [43] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [44] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [45] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [46] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [47] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [48] "18\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [49] "45\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [50] "46\t1\t0.0\t4\t0 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [51] "84\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [52] "86\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [53] "96\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [54] "121\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [55] "133\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [56] "160\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## 
## [[74]]
##  [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/BT1BCont1_S77_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/BT1BCont1_S77_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/BT1BCont1_S77_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/BT1BCont1_S77_L001_R2_001.fastq.gz"
##  [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [4] "Finished in 0.02 s (266 us/read; 0.23 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [8] "Total read pairs processed:                 64"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [9] "  Read 1 with adapter:                       1 (1.6%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [10] "  Read 2 with adapter:                       6 (9.4%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [11] "Pairs written (passing filters):            64 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [13] "Total basepairs processed:        26,880 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [14] "  Read 1:        12,544 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [15] "  Read 2:        14,336 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [16] "Total written (filtered):         26,346 bp (98.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [17] "  Read 1:        12,534 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [18] "  Read 2:        13,812 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [24] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [25] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [26] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [27] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [28] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [29] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [30] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [31] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [32] "  A: 100.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [33] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [34] "  G: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [35] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [36] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [38] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [39] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [40] "10\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [41] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [42] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [43] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [44] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [45] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [46] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [47] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [48] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [49] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 6 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [50] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [51] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [52] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [53] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [54] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [55] "  A: 83.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [56] "  C: 16.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [57] "  G: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [58] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [59] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [60] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [61] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [62] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [63] "28\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [64] "64\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [65] "70\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [66] "115\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [67] "123\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [68] "124\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## 
## [[75]]
##  [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/BT1BCont2_S78_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/BT1BCont2_S78_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/BT1BCont2_S78_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/BT1BCont2_S78_L001_R2_001.fastq.gz"
##  [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [4] "Finished in 0.02 s (270 us/read; 0.22 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [8] "Total read pairs processed:                 66"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [9] "  Read 1 with adapter:                       0 (0.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [10] "  Read 2 with adapter:                      11 (16.7%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [11] "Pairs written (passing filters):            66 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [13] "Total basepairs processed:        27,720 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [14] "  Read 1:        12,936 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [15] "  Read 2:        14,784 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [16] "Total written (filtered):         26,930 bp (97.2%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [17] "  Read 1:        12,936 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [18] "  Read 2:        13,994 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [24] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [25] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [26] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [27] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [28] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [29] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [30] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [32] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [34] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 11 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [36] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [37] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [39] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [40] "  A: 81.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [41] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [42] "  G: 18.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [43] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [44] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [45] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [46] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [47] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [48] "15\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [49] "30\t1\t0.0\t3\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [50] "50\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [51] "56\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [52] "64\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [53] "79\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [54] "92\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [55] "95\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [56] "96\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [57] "103\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [58] "110\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## 
## [[76]]
##  [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/BT1BCont3_S79_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/BT1BCont3_S79_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/BT1BCont3_S79_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/BT1BCont3_S79_L001_R2_001.fastq.gz"
##  [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [4] "Finished in 0.02 s (262 us/read; 0.23 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [8] "Total read pairs processed:                 60"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [9] "  Read 1 with adapter:                       1 (1.7%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [10] "  Read 2 with adapter:                       7 (11.7%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [11] "Pairs written (passing filters):            60 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [13] "Total basepairs processed:        25,200 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [14] "  Read 1:        11,760 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [15] "  Read 2:        13,440 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [16] "Total written (filtered):         24,595 bp (97.6%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [17] "  Read 1:        11,750 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [18] "  Read 2:        12,845 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [29] "10\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [30] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [32] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [34] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [36] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [38] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [39] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [40] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [41] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [42] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 7 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [43] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [44] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [45] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [46] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [47] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [48] "  A: 57.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [49] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [50] "  G: 42.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [51] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [52] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [53] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [54] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [55] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [56] "34\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [57] "48\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [58] "58\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [59] "62\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [60] "102\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [61] "126\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [62] "165\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## 
## [[77]]
##  [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/BT1BContRNA1_S80_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/BT1BContRNA1_S80_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/BT1BContRNA1_S80_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/BT1BContRNA1_S80_L001_R2_001.fastq.gz"
##  [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [4] "Finished in 0.04 s (187 us/read; 0.32 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [8] "Total read pairs processed:                197"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [9] "  Read 1 with adapter:                       2 (1.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [10] "  Read 2 with adapter:                      11 (5.6%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [11] "Pairs written (passing filters):           197 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [13] "Total basepairs processed:        82,740 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [14] "  Read 1:        38,612 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [15] "  Read 2:        44,128 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [16] "Total written (filtered):         81,732 bp (98.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [17] "  Read 1:        38,418 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [18] "  Read 2:        43,314 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [24] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [25] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [26] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 2 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [27] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [28] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [29] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [30] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [31] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [32] "  A: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [33] "  C: 50.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [34] "  G: 50.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [35] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [36] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [38] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [39] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [40] "51\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [41] "143\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [42] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [43] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [44] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [45] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [46] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [47] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [48] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [49] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [50] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 11 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [51] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [52] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [53] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [54] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [55] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [56] "  A: 72.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [57] "  C: 9.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [58] "  G: 9.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [59] "  T: 9.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [60] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [61] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [62] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [63] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [64] "11\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [65] "39\t1\t0.0\t3\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [66] "43\t1\t0.0\t4\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [67] "52\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [68] "58\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [69] "66\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [70] "78\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [71] "89\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [72] "102\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [73] "105\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [74] "171\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## 
## [[78]]
##  [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/BT1BContRNA2_S81_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/BT1BContRNA2_S81_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/BT1BContRNA2_S81_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/BT1BContRNA2_S81_L001_R2_001.fastq.gz"
##  [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [4] "Finished in 0.02 s (201 us/read; 0.30 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [8] "Total read pairs processed:                111"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [9] "  Read 1 with adapter:                       1 (0.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [10] "  Read 2 with adapter:                       6 (5.4%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [11] "Pairs written (passing filters):           111 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [13] "Total basepairs processed:        46,620 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [14] "  Read 1:        21,756 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [15] "  Read 2:        24,864 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [16] "Total written (filtered):         46,131 bp (99.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [17] "  Read 1:        21,736 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [18] "  Read 2:        24,395 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [29] "20\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [30] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [32] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [34] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [36] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [38] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [39] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [40] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [41] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [42] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 6 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [43] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [44] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [45] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [46] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [47] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [48] "  A: 33.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [49] "  C: 16.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [50] "  G: 50.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [51] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [52] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [53] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [54] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [55] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [56] "22\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [57] "42\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [58] "43\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [59] "108\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [60] "114\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [61] "140\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## 
## [[79]]
##  [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/BT1BContRNA3_S82_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/BT1BContRNA3_S82_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/BT1BContRNA3_S82_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/BT1BContRNA3_S82_L001_R2_001.fastq.gz"
##  [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [4] "Finished in 0.08 s (143 us/read; 0.42 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [8] "Total read pairs processed:                563"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [9] "  Read 1 with adapter:                       0 (0.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [10] "  Read 2 with adapter:                      24 (4.3%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [11] "Pairs written (passing filters):           563 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [13] "Total basepairs processed:       236,460 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [14] "  Read 1:       110,348 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [15] "  Read 2:       126,112 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [16] "Total written (filtered):        234,801 bp (99.3%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [17] "  Read 1:       110,348 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [18] "  Read 2:       124,453 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [24] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [25] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [26] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [27] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [28] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [29] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [30] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [32] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [34] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 24 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [36] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [37] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [39] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [40] "  A: 70.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [41] "  C: 16.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [42] "  G: 8.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [43] "  T: 4.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [44] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [45] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [46] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [47] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [48] "11\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [49] "14\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [50] "17\t3\t0.0\t1\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [51] "23\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [52] "37\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [53] "42\t1\t0.0\t4\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [54] "49\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [55] "58\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [56] "61\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [57] "65\t2\t0.0\t4\t0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [58] "73\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [59] "74\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [60] "77\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [61] "83\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [62] "96\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [63] "98\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [64] "121\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [65] "124\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [66] "134\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [67] "149\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [68] "154\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## 
## [[80]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/BT1BRNA1_S74_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/BT1BRNA1_S74_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/BT1BRNA1_S74_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/BT1BRNA1_S74_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##   [4] "Finished in 0.95 s (123 us/read; 0.49 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [8] "Total read pairs processed:              7,702"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [9] "  Read 1 with adapter:                      75 (1.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [10] "  Read 2 with adapter:                     199 (2.6%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [11] "Pairs written (passing filters):         7,701 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [13] "Total basepairs processed:     3,234,840 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [14] "  Read 1:     1,509,592 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [15] "  Read 2:     1,725,248 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [16] "Total written (filtered):      3,210,764 bp (99.3%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [17] "  Read 1:     1,503,310 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [18] "  Read 2:     1,707,454 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 2 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [29] "18\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [30] "24\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [32] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [33] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [34] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [35] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 73 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [36] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [37] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [38] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [39] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [40] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [41] "  A: 42.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [42] "  C: 5.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [43] "  G: 30.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [44] "  T: 21.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [45] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [46] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [47] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [48] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [49] "30\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [50] "36\t11\t0.0\t2\t11"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [51] "74\t16\t0.0\t2\t16"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [52] "75\t14\t0.0\t2\t13 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [53] "82\t4\t0.0\t2\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [54] "90\t5\t0.0\t2\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [55] "91\t4\t0.0\t2\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [56] "122\t16\t0.0\t2\t16"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [57] "144\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [58] "146\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [59] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [60] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [61] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [62] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [63] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [64] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [65] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [66] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [67] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 199 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [68] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [69] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [70] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [71] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [72] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [73] "  A: 94.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [74] "  C: 3.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [75] "  G: 2.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [76] "  T: 0.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [77] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [78] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [79] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [80] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [81] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [82] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [83] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [84] "5\t1\t7.5\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [85] "7\t1\t0.5\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [86] "9\t1\t0.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [87] "11\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [88] "13\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [89] "14\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [90] "15\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [91] "16\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [92] "21\t3\t0.0\t2\t0 1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [93] "23\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [94] "24\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [95] "25\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [96] "27\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [97] "29\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [98] "30\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [99] "31\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [100] "33\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [101] "34\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [102] "35\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [103] "37\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [104] "38\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [105] "39\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [106] "40\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [107] "42\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [108] "43\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [109] "44\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [110] "47\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [111] "48\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [112] "51\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [113] "53\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [114] "54\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [115] "55\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [116] "56\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [117] "58\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [118] "59\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [119] "60\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [120] "61\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [121] "63\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [122] "64\t12\t0.0\t4\t12"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [123] "68\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [124] "69\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [125] "73\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [126] "74\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [127] "75\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [128] "76\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [129] "77\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [130] "78\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [131] "79\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [132] "80\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [133] "82\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [134] "83\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [135] "84\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [136] "85\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [137] "86\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [138] "88\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [139] "89\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [140] "91\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [141] "94\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [142] "97\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [143] "99\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [144] "100\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [145] "102\t16\t0.0\t4\t16"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [146] "103\t14\t0.0\t4\t14"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [147] "104\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [148] "106\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [149] "107\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [150] "108\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [151] "110\t7\t0.0\t4\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [152] "111\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [153] "112\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [154] "117\t2\t0.0\t4\t0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [155] "118\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [156] "119\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [157] "120\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [158] "126\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [159] "127\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [160] "129\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [161] "135\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [162] "136\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [163] "139\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [164] "141\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [165] "142\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [166] "146\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [167] "148\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [168] "149\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [169] "150\t16\t0.0\t4\t16"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [170] "161\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [171] "162\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [172] "167\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [173] "170\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [174] "172\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [175] "174\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [176] "175\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [177] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [178] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [179] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [180] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [181] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## 
## [[81]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/BT1BRNA2_S75_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/BT1BRNA2_S75_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/BT1BRNA2_S75_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/BT1BRNA2_S75_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##   [4] "Finished in 0.09 s (134 us/read; 0.45 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [8] "Total read pairs processed:                707"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [9] "  Read 1 with adapter:                      31 (4.4%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [10] "  Read 2 with adapter:                      49 (6.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [11] "Pairs written (passing filters):           705 (99.7%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [13] "Total basepairs processed:       296,940 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [14] "  Read 1:       138,572 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [15] "  Read 2:       158,368 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [16] "Total written (filtered):        289,084 bp (97.4%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [17] "  Read 1:       135,649 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [18] "  Read 2:       153,435 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [29] "28\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [30] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [32] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [34] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 30 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [36] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [37] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [39] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [40] "  A: 10.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [41] "  C: 30.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [42] "  G: 26.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [43] "  T: 33.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [44] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [45] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [46] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [47] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [48] "6\t1\t0.2\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [49] "82\t21\t0.0\t2\t21"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [50] "83\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [51] "129\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [52] "139\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [53] "146\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [54] "157\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [55] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [56] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [57] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [58] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [59] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [60] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [61] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [62] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [63] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 49 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [64] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [65] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [66] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [67] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [68] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [69] "  A: 87.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [70] "  C: 8.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [71] "  G: 4.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [72] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [73] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [74] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [75] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [76] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [77] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [78] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [79] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [80] "5\t2\t0.7\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [81] "6\t1\t0.2\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [82] "8\t1\t0.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [83] "25\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [84] "27\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [85] "34\t2\t0.0\t3\t0 0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [86] "35\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [87] "44\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [88] "57\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [89] "78\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [90] "86\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [91] "87\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [92] "90\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [93] "110\t22\t0.0\t4\t5 16 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [94] "119\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [95] "136\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [96] "146\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [97] "157\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [98] "167\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [99] "174\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [100] "183\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [101] "184\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [102] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [103] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [104] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [105] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [106] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## 
## [[82]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/BT1BRNA3_S76_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/BT1BRNA3_S76_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/BT1BRNA3_S76_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/BT1BRNA3_S76_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##   [4] "Finished in 0.63 s (121 us/read; 0.50 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [8] "Total read pairs processed:              5,171"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [9] "  Read 1 with adapter:                      73 (1.4%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [10] "  Read 2 with adapter:                     152 (2.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [11] "Pairs written (passing filters):         5,171 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [13] "Total basepairs processed:     2,171,820 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [14] "  Read 1:     1,013,516 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [15] "  Read 2:     1,158,304 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [16] "Total written (filtered):      2,153,167 bp (99.1%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [17] "  Read 1:     1,008,027 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [18] "  Read 2:     1,145,140 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 2 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [29] "6\t1\t1.3\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [30] "13\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [32] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [33] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [34] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [35] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 71 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [36] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [37] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [38] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [39] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [40] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [41] "  A: 12.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [42] "  C: 14.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [43] "  G: 73.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [44] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [45] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [46] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [47] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [48] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [49] "19\t6\t0.0\t1\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [50] "29\t12\t0.0\t2\t11 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [51] "30\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [52] "62\t8\t0.0\t2\t8"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [53] "63\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [54] "70\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [55] "74\t10\t0.0\t2\t9 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [56] "82\t5\t0.0\t2\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [57] "83\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [58] "92\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [59] "93\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [60] "110\t6\t0.0\t2\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [61] "111\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [62] "142\t9\t0.0\t2\t9"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [63] "143\t4\t0.0\t2\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [64] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [65] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [66] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [67] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [68] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [69] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [70] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [71] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [72] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 152 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [73] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [74] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [75] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [76] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [77] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [78] "  A: 94.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [79] "  C: 3.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [80] "  G: 1.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [81] "  T: 0.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [82] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [83] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [84] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [85] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [86] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [87] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [88] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [89] "5\t3\t5.0\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [90] "6\t1\t1.3\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [91] "7\t1\t0.3\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [92] "8\t1\t0.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [93] "14\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [94] "15\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [95] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [96] "19\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [97] "23\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [98] "29\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [99] "34\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [100] "37\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [101] "38\t4\t0.0\t3\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [102] "39\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [103] "41\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [104] "42\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [105] "44\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [106] "45\t2\t0.0\t4\t0 1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [107] "47\t9\t0.0\t4\t6 1 0 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [108] "48\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [109] "49\t2\t0.0\t4\t1 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [110] "57\t14\t0.0\t4\t12 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [111] "58\t1\t0.0\t4\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [112] "59\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [113] "67\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [114] "73\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [115] "74\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [116] "77\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [117] "78\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [118] "79\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [119] "81\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [120] "82\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [121] "83\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [122] "84\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [123] "85\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [124] "88\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [125] "89\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [126] "90\t12\t0.0\t4\t11 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [127] "91\t3\t0.0\t4\t1 0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [128] "95\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [129] "97\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [130] "98\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [131] "102\t11\t0.0\t4\t9 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [132] "110\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [133] "111\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [134] "112\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [135] "120\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [136] "127\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [137] "128\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [138] "133\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [139] "134\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [140] "136\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [141] "138\t8\t0.0\t4\t6 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [142] "140\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [143] "144\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [144] "146\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [145] "158\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [146] "162\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [147] "166\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [148] "170\t9\t0.0\t4\t9"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [149] "171\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [150] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [151] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [152] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [153] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [154] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## 
## [[83]]
##  [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/ExtC1_S83_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/ExtC1_S83_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/ExtC1_S83_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/ExtC1_S83_L001_R2_001.fastq.gz"
##  [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [4] "Finished in 0.23 s (119 us/read; 0.50 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [8] "Total read pairs processed:              1,945"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [9] "  Read 1 with adapter:                       5 (0.3%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [10] "  Read 2 with adapter:                      20 (1.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [11] "Pairs written (passing filters):         1,945 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [13] "Total basepairs processed:       816,900 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [14] "  Read 1:       381,220 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [15] "  Read 2:       435,680 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [16] "Total written (filtered):        815,193 bp (99.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [17] "  Read 1:       380,770 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [18] "  Read 2:       434,423 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [29] "5\t1\t1.9\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [30] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [32] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [34] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 4 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [36] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [37] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [39] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [40] "  A: 25.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [41] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [42] "  G: 75.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [43] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [44] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [45] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [46] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [47] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [48] "34\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [49] "128\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [50] "141\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [51] "142\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [52] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [53] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [54] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [55] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [56] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [57] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [58] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [59] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [60] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 20 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [61] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [62] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [63] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [64] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [65] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [66] "  A: 85.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [67] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [68] "  G: 15.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [69] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [70] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [71] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [72] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [73] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [74] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [75] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [76] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [77] "5\t1\t1.9\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [78] "16\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [79] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [80] "25\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [81] "29\t1\t0.0\t2\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [82] "34\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [83] "45\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [84] "46\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [85] "50\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [86] "61\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [87] "71\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [88] "77\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [89] "78\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [90] "81\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [91] "90\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [92] "121\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [93] "141\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [94] "164\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [95] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [96] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [97] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [98] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [99] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## 
## [[84]]
##  [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/ExtC2_S84_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/ExtC2_S84_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/ExtC2_S84_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/ExtC2_S84_L001_R2_001.fastq.gz"
##  [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [4] "Finished in 0.16 s (119 us/read; 0.50 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [8] "Total read pairs processed:              1,307"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [9] "  Read 1 with adapter:                       0 (0.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [10] "  Read 2 with adapter:                      20 (1.5%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [11] "Pairs written (passing filters):         1,307 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [13] "Total basepairs processed:       548,940 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [14] "  Read 1:       256,172 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [15] "  Read 2:       292,768 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [16] "Total written (filtered):        547,679 bp (99.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [17] "  Read 1:       256,172 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [18] "  Read 2:       291,507 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [24] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [25] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [26] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [27] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [28] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [29] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [30] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [32] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [34] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 20 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [36] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [37] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [39] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [40] "  A: 100.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [41] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [42] "  G: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [43] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [44] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [45] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [46] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [47] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [48] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [49] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [50] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [51] "8\t2\t0.0\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [52] "15\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [53] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [54] "30\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [55] "32\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [56] "35\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [57] "37\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [58] "43\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [59] "61\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [60] "63\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [61] "79\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [62] "82\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [63] "103\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [64] "106\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [65] "110\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [66] "111\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [67] "127\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [68] "157\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [69] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [70] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [71] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [72] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [73] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## 
## [[85]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/ExtC3_S85_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/ExtC3_S85_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/ExtC3_S85_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/ExtC3_S85_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##   [4] "Finished in 0.28 s (122 us/read; 0.49 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##   [8] "Total read pairs processed:              2,266"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##   [9] "  Read 1 with adapter:                       3 (0.1%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [10] "  Read 2 with adapter:                      37 (1.6%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [11] "Pairs written (passing filters):         2,266 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [13] "Total basepairs processed:       951,720 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [14] "  Read 1:       444,136 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [15] "  Read 2:       507,584 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [16] "Total written (filtered):        948,968 bp (99.7%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [17] "  Read 1:       443,841 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [18] "  Read 2:       505,127 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [29] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [30] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [32] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [34] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 2 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [36] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [37] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [39] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [40] "  A: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [41] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [42] "  G: 100.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [43] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [44] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [45] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [46] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [47] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [48] "139\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [49] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [50] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [51] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [52] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [53] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [54] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [55] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [56] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [57] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 37 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [58] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [59] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [60] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [61] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [62] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [63] "  A: 75.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [64] "  C: 16.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [65] "  G: 8.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [66] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [67] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [68] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [69] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [70] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [71] "5\t1\t2.2\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [72] "7\t1\t0.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [73] "8\t2\t0.0\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [74] "14\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [75] "30\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [76] "32\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [77] "41\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [78] "48\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [79] "51\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [80] "53\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [81] "57\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [82] "58\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [83] "60\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [84] "61\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [85] "63\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [86] "72\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [87] "76\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [88] "79\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [89] "87\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [90] "88\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [91] "91\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [92] "98\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [93] "102\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [94] "104\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [95] "105\t1\t0.0\t4\t0 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [96] "115\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [97] "131\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [98] "136\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [99] "141\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [100] "164\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## 
## [[86]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/ExtC4_S86_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/ExtC4_S86_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/ExtC4_S86_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/ExtC4_S86_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##   [4] "Finished in 0.57 s (112 us/read; 0.53 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##   [8] "Total read pairs processed:              5,074"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##   [9] "  Read 1 with adapter:                       4 (0.1%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [10] "  Read 2 with adapter:                      45 (0.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [11] "Pairs written (passing filters):         5,074 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [13] "Total basepairs processed:     2,131,080 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [14] "  Read 1:       994,504 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [15] "  Read 2:     1,136,576 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [16] "Total written (filtered):      2,128,130 bp (99.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [17] "  Read 1:       994,314 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [18] "  Read 2:     1,133,816 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 3 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [29] "5\t1\t5.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [30] "12\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [31] "36\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [32] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [34] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [36] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [38] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [39] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [40] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [41] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [42] "  A: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [43] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [44] "  G: 100.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [45] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [46] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [47] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [48] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [49] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [50] "137\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [51] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [52] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [53] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [54] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [55] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [56] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [57] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [58] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [59] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 45 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [60] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [61] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [62] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [63] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [64] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [65] "  A: 95.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [66] "  C: 2.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [67] "  G: 2.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [68] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [69] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [70] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [71] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [72] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [73] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [74] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [75] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [76] "5\t1\t5.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [77] "6\t1\t1.2\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [78] "8\t2\t0.1\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [79] "9\t1\t0.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [80] "10\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [81] "15\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [82] "16\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [83] "19\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [84] "20\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [85] "28\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [86] "29\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [87] "32\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [88] "38\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [89] "42\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [90] "45\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [91] "46\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [92] "50\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [93] "58\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [94] "59\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [95] "70\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [96] "71\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [97] "72\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [98] "73\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [99] "74\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [100] "75\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [101] "76\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [102] "79\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [103] "80\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [104] "81\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [105] "82\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [106] "83\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [107] "84\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [108] "88\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [109] "93\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [110] "100\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [111] "104\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [112] "105\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [113] "109\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [114] "110\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [115] "114\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [116] "115\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [117] "126\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [118] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [119] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [120] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [121] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [122] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## 
## [[87]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/ExtC5_S87_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/ExtC5_S87_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/ExtC5_S87_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/ExtC5_S87_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##   [4] "Finished in 1.33 s (116 us/read; 0.52 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##   [8] "Total read pairs processed:             11,476"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##   [9] "  Read 1 with adapter:                       4 (0.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [10] "  Read 2 with adapter:                      65 (0.6%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [11] "Pairs written (passing filters):        11,476 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [13] "Total basepairs processed:     4,819,920 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [14] "  Read 1:     2,249,296 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [15] "  Read 2:     2,570,624 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [16] "Total written (filtered):      4,815,511 bp (99.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [17] "  Read 1:     2,249,190 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [18] "  Read 2:     2,566,321 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 3 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [29] "8\t1\t0.2\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [30] "9\t1\t0.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [31] "13\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [32] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [34] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [36] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [38] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [39] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [40] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [41] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [42] "  A: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [43] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [44] "  G: 100.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [45] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [46] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [47] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [48] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [49] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [50] "76\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [51] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [52] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [53] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [54] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [55] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [56] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [57] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [58] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [59] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 65 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [60] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [61] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [62] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [63] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [64] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [65] "  A: 92.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [66] "  C: 1.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [67] "  G: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [68] "  T: 6.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [69] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [70] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [71] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [72] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [73] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [74] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [75] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [76] "5\t3\t11.2\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [77] "8\t2\t0.2\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [78] "9\t1\t0.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [79] "14\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [80] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [81] "18\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [82] "20\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [83] "22\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [84] "23\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [85] "25\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [86] "27\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [87] "29\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [88] "31\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [89] "32\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [90] "35\t3\t0.0\t3\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [91] "40\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [92] "41\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [93] "53\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [94] "54\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [95] "58\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [96] "59\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [97] "65\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [98] "67\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [99] "68\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [100] "69\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [101] "71\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [102] "72\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [103] "74\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [104] "75\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [105] "78\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [106] "81\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [107] "82\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [108] "83\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [109] "84\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [110] "85\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [111] "86\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [112] "87\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [113] "91\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [114] "99\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [115] "101\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [116] "104\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [117] "105\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [118] "111\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [119] "120\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [120] "127\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [121] "140\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [122] "141\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [123] "145\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [124] "150\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [125] "159\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [126] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [127] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [128] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [129] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [130] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## 
## [[88]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/ExtC6_S88_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/ExtC6_S88_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/ExtC6_S88_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/ExtC6_S88_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##   [4] "Finished in 0.66 s (110 us/read; 0.55 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##   [8] "Total read pairs processed:              6,024"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##   [9] "  Read 1 with adapter:                       6 (0.1%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [10] "  Read 2 with adapter:                      52 (0.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [11] "Pairs written (passing filters):         6,024 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [13] "Total basepairs processed:     2,530,080 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [14] "  Read 1:     1,180,704 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [15] "  Read 2:     1,349,376 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [16] "Total written (filtered):      2,526,032 bp (99.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [17] "  Read 1:     1,180,496 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [18] "  Read 2:     1,345,536 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 3 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [29] "8\t1\t0.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [30] "11\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [31] "21\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [32] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [34] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [36] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 3 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [38] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [39] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [40] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [41] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [42] "  A: 66.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [43] "  C: 33.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [44] "  G: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [45] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [46] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [47] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [48] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [49] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [50] "6\t1\t1.5\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [51] "80\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [52] "82\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [53] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [54] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [55] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [56] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [57] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [58] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [59] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [60] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [61] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [62] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [63] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [64] "5\t1\t5.9\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [65] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [66] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [67] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [68] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [69] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 51 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [70] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [71] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [72] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [73] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [74] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [75] "  A: 92.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [76] "  C: 2.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [77] "  G: 5.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [78] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [79] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [80] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [81] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [82] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [83] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [84] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [85] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [86] "7\t1\t0.4\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [87] "8\t1\t0.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [88] "12\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [89] "18\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [90] "20\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [91] "28\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [92] "34\t2\t0.0\t3\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [93] "37\t1\t0.0\t3\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [94] "41\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [95] "42\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [96] "49\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [97] "51\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [98] "54\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [99] "58\t1\t0.0\t4\t0 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [100] "59\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [101] "63\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [102] "64\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [103] "66\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [104] "73\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [105] "75\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [106] "79\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [107] "84\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [108] "85\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [109] "87\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [110] "88\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [111] "90\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [112] "93\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [113] "94\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [114] "96\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [115] "99\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [116] "105\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [117] "108\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [118] "109\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [119] "110\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [120] "111\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [121] "113\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [122] "128\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [123] "143\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [124] "146\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [125] "162\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [126] "165\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [127] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [128] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [129] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [130] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [131] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## 
## [[89]]
##  [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/ExtCRNA1_S89_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/ExtCRNA1_S89_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/ExtCRNA1_S89_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/ExtCRNA1_S89_L001_R2_001.fastq.gz"
##  [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [4] "Finished in 0.03 s (199 us/read; 0.30 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [8] "Total read pairs processed:                129"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [9] "  Read 1 with adapter:                       0 (0.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [10] "  Read 2 with adapter:                       9 (7.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [11] "Pairs written (passing filters):           129 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [13] "Total basepairs processed:        54,180 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [14] "  Read 1:        25,284 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [15] "  Read 2:        28,896 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [16] "Total written (filtered):         53,555 bp (98.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [17] "  Read 1:        25,284 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [18] "  Read 2:        28,271 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [24] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [25] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [26] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [27] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [28] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [29] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [30] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [32] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [34] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 9 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [36] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [37] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [39] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [40] "  A: 88.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [41] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [42] "  G: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [43] "  T: 11.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [44] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [45] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [46] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [47] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [48] "5\t1\t0.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [49] "8\t1\t0.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [50] "41\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [51] "52\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [52] "63\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [53] "90\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [54] "92\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [55] "111\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [56] "163\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## 
## [[90]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/ExtCRNA2_S90_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/ExtCRNA2_S90_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/ExtCRNA2_S90_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/ExtCRNA2_S90_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##   [4] "Finished in 0.61 s (97 us/read; 0.62 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [8] "Total read pairs processed:              6,230"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [9] "  Read 1 with adapter:                       0 (0.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [10] "  Read 2 with adapter:                      85 (1.4%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [11] "Pairs written (passing filters):         6,230 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [13] "Total basepairs processed:     2,616,600 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [14] "  Read 1:     1,221,080 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [15] "  Read 2:     1,395,520 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [16] "Total written (filtered):      2,608,786 bp (99.7%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [17] "  Read 1:     1,221,080 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [18] "  Read 2:     1,387,706 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [24] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [25] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [26] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [27] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [28] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [29] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [30] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [32] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [34] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 85 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [36] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [37] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [39] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [40] "  A: 97.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [41] "  C: 1.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [42] "  G: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [43] "  T: 1.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [44] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [45] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [46] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [47] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [48] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [49] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [50] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [51] "8\t1\t0.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [52] "12\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [53] "16\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [54] "30\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [55] "33\t4\t0.0\t3\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [56] "36\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [57] "38\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [58] "42\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [59] "43\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [60] "45\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [61] "46\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [62] "47\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [63] "52\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [64] "57\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [65] "58\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [66] "60\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [67] "63\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [68] "65\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [69] "66\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [70] "72\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [71] "73\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [72] "74\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [73] "78\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [74] "79\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [75] "80\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [76] "82\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [77] "85\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [78] "86\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [79] "88\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [80] "91\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [81] "94\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [82] "96\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [83] "98\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [84] "102\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [85] "103\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [86] "104\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [87] "106\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [88] "107\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [89] "109\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [90] "110\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [91] "111\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [92] "113\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [93] "118\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [94] "120\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [95] "123\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [96] "126\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [97] "127\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [98] "129\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [99] "133\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [100] "139\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [101] "144\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [102] "146\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [103] "151\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [104] "152\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [105] "157\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [106] "158\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [107] "160\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [108] "166\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [109] "173\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [110] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [111] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [112] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [113] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [114] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## 
## [[91]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/ExtCRNA3_S91_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/ExtCRNA3_S91_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/ExtCRNA3_S91_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/ExtCRNA3_S91_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##   [4] "Finished in 0.83 s (122 us/read; 0.49 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [8] "Total read pairs processed:              6,783"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [9] "  Read 1 with adapter:                      61 (0.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [10] "  Read 2 with adapter:                     181 (2.7%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [11] "Pairs written (passing filters):         6,780 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [13] "Total basepairs processed:     2,848,860 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [14] "  Read 1:     1,329,468 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [15] "  Read 2:     1,519,392 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [16] "Total written (filtered):      2,832,078 bp (99.4%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [17] "  Read 1:     1,326,106 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [18] "  Read 2:     1,505,972 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [29] "5\t1\t6.6\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [30] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [32] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [34] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 60 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [36] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [37] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [39] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [40] "  A: 40.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [41] "  C: 5.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [42] "  G: 55.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [43] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [44] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [45] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [46] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [47] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [48] "16\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [49] "17\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [50] "19\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [51] "23\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [52] "36\t27\t0.0\t2\t27"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [53] "42\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [54] "60\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [55] "61\t12\t0.0\t2\t11 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [56] "64\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [57] "75\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [58] "76\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [59] "82\t4\t0.0\t2\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [60] "93\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [61] "142\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [62] "156\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [63] "160\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [64] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [65] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [66] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [67] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [68] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [69] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [70] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [71] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [72] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [73] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [74] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [75] "6\t1\t1.7\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [76] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [77] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [78] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [79] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [80] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 180 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [81] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [82] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [83] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [84] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [85] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [86] "  A: 88.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [87] "  C: 4.4%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [88] "  G: 7.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [89] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [90] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [91] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [92] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [93] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [94] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [95] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [96] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [97] "5\t2\t6.6\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [98] "6\t2\t1.7\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [99] "7\t1\t0.4\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [100] "8\t2\t0.1\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [101] "10\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [102] "12\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [103] "13\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [104] "16\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [105] "19\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [106] "22\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [107] "24\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [108] "25\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [109] "27\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [110] "28\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [111] "32\t1\t0.0\t3\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [112] "33\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [113] "34\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [114] "35\t2\t0.0\t3\t0 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [115] "39\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [116] "41\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [117] "42\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [118] "43\t1\t0.0\t4\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [119] "44\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [120] "45\t6\t0.0\t4\t3 2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [121] "46\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [122] "47\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [123] "49\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [124] "51\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [125] "52\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [126] "53\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [127] "55\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [128] "57\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [129] "59\t5\t0.0\t4\t3 0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [130] "60\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [131] "61\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [132] "62\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [133] "63\t2\t0.0\t4\t1 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [134] "64\t28\t0.0\t4\t25 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [135] "65\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [136] "68\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [137] "70\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [138] "71\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [139] "73\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [140] "74\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [141] "77\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [142] "79\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [143] "81\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [144] "82\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [145] "84\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [146] "86\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [147] "87\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [148] "88\t3\t0.0\t4\t1 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [149] "89\t14\t0.0\t4\t2 12"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [150] "90\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [151] "91\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [152] "92\t2\t0.0\t4\t1 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [153] "93\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [154] "94\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [155] "95\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [156] "96\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [157] "98\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [158] "99\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [159] "103\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [160] "104\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [161] "105\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [162] "106\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [163] "110\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [164] "115\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [165] "116\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [166] "118\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [167] "121\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [168] "122\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [169] "123\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [170] "130\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [171] "133\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [172] "137\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [173] "145\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [174] "146\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [175] "147\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [176] "150\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [177] "152\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [178] "154\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [179] "155\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [180] "166\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [181] "172\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [182] "176\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [183] "184\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [184] "188\t1\t0.0\t4\t0 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [185] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [186] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [187] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [188] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [189] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## 
## [[92]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/ExtCRNA4_S92_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/ExtCRNA4_S92_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/ExtCRNA4_S92_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/ExtCRNA4_S92_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##   [4] "Finished in 0.44 s (124 us/read; 0.49 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [8] "Total read pairs processed:              3,551"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [9] "  Read 1 with adapter:                      60 (1.7%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [10] "  Read 2 with adapter:                     123 (3.5%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [11] "Pairs written (passing filters):         3,551 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [13] "Total basepairs processed:     1,491,420 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [14] "  Read 1:       695,996 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [15] "  Read 2:       795,424 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [16] "Total written (filtered):      1,475,370 bp (98.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [17] "  Read 1:       691,075 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [18] "  Read 2:       784,295 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [24] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [25] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [26] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 60 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [27] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [28] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [29] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [30] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [31] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [32] "  A: 36.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [33] "  C: 35.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [34] "  G: 28.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [35] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [36] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [38] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [39] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [40] "10\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [41] "11\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [42] "12\t4\t0.0\t1\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [43] "62\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [44] "63\t8\t0.0\t2\t7 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [45] "82\t20\t0.0\t2\t19 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [46] "83\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [47] "90\t5\t0.0\t2\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [48] "95\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [49] "96\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [50] "113\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [51] "117\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [52] "122\t11\t0.0\t2\t11"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [53] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [54] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [55] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [56] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [57] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [58] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [59] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [60] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [61] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 123 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [62] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [63] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [64] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [65] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [66] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [67] "  A: 90.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [68] "  C: 8.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [69] "  G: 0.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [70] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [71] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [72] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [73] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [74] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [75] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [76] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [77] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [78] "5\t1\t3.5\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [79] "8\t1\t0.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [80] "14\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [81] "16\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [82] "19\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [83] "20\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [84] "21\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [85] "22\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [86] "23\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [87] "28\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [88] "30\t1\t0.0\t3\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [89] "32\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [90] "34\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [91] "35\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [92] "36\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [93] "38\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [94] "40\t6\t0.0\t4\t3 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [95] "45\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [96] "48\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [97] "51\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [98] "57\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [99] "62\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [100] "63\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [101] "72\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [102] "74\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [103] "75\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [104] "78\t2\t0.0\t4\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [105] "79\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [106] "80\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [107] "84\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [108] "85\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [109] "86\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [110] "87\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [111] "89\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [112] "90\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [113] "91\t8\t0.0\t4\t7 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [114] "92\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [115] "95\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [116] "100\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [117] "101\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [118] "108\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [119] "110\t20\t0.0\t4\t20"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [120] "111\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [121] "114\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [122] "117\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [123] "118\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [124] "119\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [125] "121\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [126] "123\t2\t0.0\t4\t0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [127] "127\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [128] "128\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [129] "129\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [130] "141\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [131] "145\t1\t0.0\t4\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [132] "146\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [133] "147\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [134] "150\t11\t0.0\t4\t11"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [135] "164\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [136] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [137] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [138] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [139] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [140] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## 
## [[93]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/ExtCRNA5_S93_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/ExtCRNA5_S93_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/ExtCRNA5_S93_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/ExtCRNA5_S93_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##   [4] "Finished in 0.54 s (122 us/read; 0.49 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [8] "Total read pairs processed:              4,468"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [9] "  Read 1 with adapter:                       3 (0.1%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [10] "  Read 2 with adapter:                     114 (2.6%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [11] "Pairs written (passing filters):         4,467 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [13] "Total basepairs processed:     1,876,560 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [14] "  Read 1:       875,728 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [15] "  Read 2:     1,000,832 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [16] "Total written (filtered):      1,868,356 bp (99.6%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [17] "  Read 1:       875,420 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [18] "  Read 2:       992,936 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [24] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [25] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [26] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 3 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [27] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [28] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [29] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [30] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [31] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [32] "  A: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [33] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [34] "  G: 100.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [35] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [36] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [38] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [39] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [40] "19\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [41] "36\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [42] "57\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [43] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [44] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [45] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [46] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [47] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [48] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [49] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [50] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [51] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 114 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [52] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [53] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [54] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [55] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [56] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [57] "  A: 91.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [58] "  C: 6.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [59] "  G: 1.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [60] "  T: 0.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [61] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [62] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [63] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [64] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [65] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [66] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [67] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [68] "5\t3\t4.4\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [69] "6\t2\t1.1\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [70] "7\t1\t0.3\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [71] "9\t1\t0.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [72] "15\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [73] "16\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [74] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [75] "18\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [76] "19\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [77] "20\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [78] "21\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [79] "22\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [80] "24\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [81] "28\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [82] "29\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [83] "30\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [84] "32\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [85] "33\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [86] "35\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [87] "36\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [88] "38\t1\t0.0\t3\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [89] "40\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [90] "42\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [91] "43\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [92] "44\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [93] "47\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [94] "48\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [95] "50\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [96] "51\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [97] "52\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [98] "57\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [99] "58\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [100] "59\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [101] "62\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [102] "64\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [103] "66\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [104] "72\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [105] "74\t4\t0.0\t4\t3 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [106] "75\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [107] "76\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [108] "78\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [109] "79\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [110] "83\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [111] "84\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [112] "85\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [113] "86\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [114] "91\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [115] "92\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [116] "94\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [117] "95\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [118] "96\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [119] "102\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [120] "103\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [121] "104\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [122] "105\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [123] "108\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [124] "110\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [125] "111\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [126] "113\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [127] "114\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [128] "115\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [129] "117\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [130] "119\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [131] "120\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [132] "130\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [133] "135\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [134] "138\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [135] "140\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [136] "142\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [137] "144\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [138] "159\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [139] "162\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [140] "181\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [141] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [142] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [143] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [144] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [145] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## 
## [[94]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/ExtCRNA6_S94_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/ExtCRNA6_S94_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/ExtCRNA6_S94_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/ExtCRNA6_S94_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##   [4] "Finished in 0.99 s (119 us/read; 0.50 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [8] "Total read pairs processed:              8,328"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [9] "  Read 1 with adapter:                     143 (1.7%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [10] "  Read 2 with adapter:                     297 (3.6%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [11] "Pairs written (passing filters):         8,321 (99.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [13] "Total basepairs processed:     3,497,760 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [14] "  Read 1:     1,632,288 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [15] "  Read 2:     1,865,472 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [16] "Total written (filtered):      3,469,016 bp (99.2%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [17] "  Read 1:     1,623,742 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [18] "  Read 2:     1,845,274 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [24] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [25] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [26] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 143 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [27] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [28] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [29] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [30] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [31] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [32] "  A: 5.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [33] "  C: 8.4%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [34] "  G: 86.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [35] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [36] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [37] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [38] "    The adapter is preceded by \"G\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [39] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [40] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [41] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [42] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [43] "16\t45\t0.0\t1\t44 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [44] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [45] "18\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [46] "34\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [47] "50\t8\t0.0\t2\t8"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [48] "51\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [49] "58\t47\t0.0\t2\t44 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [50] "62\t5\t0.0\t2\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [51] "82\t12\t0.0\t2\t12"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [52] "86\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [53] "109\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [54] "125\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [55] "133\t8\t0.0\t2\t8"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [56] "134\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [57] "146\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [58] "157\t7\t0.0\t2\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [59] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [60] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [61] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [62] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [63] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [64] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [65] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [66] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [67] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 297 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [68] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [69] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [70] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [71] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [72] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [73] "  A: 76.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [74] "  C: 19.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [75] "  G: 3.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [76] "  T: 0.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [77] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [78] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [79] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [80] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [81] "5\t5\t8.1\t0\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [82] "6\t1\t2.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [83] "7\t1\t0.5\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [84] "8\t1\t0.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [85] "12\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [86] "13\t2\t0.0\t1\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [87] "14\t3\t0.0\t1\t1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [88] "16\t50\t0.0\t1\t47 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [89] "18\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [90] "19\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [91] "20\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [92] "22\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [93] "23\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [94] "25\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [95] "28\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [96] "29\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [97] "30\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [98] "33\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [99] "34\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [100] "38\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [101] "39\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [102] "40\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [103] "42\t4\t0.0\t4\t2 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [104] "43\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [105] "44\t42\t0.0\t4\t0 36 5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [106] "45\t3\t0.0\t4\t1 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [107] "46\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [108] "47\t4\t0.0\t4\t1 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [109] "48\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [110] "52\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [111] "53\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [112] "54\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [113] "55\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [114] "58\t1\t0.0\t4\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [115] "60\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [116] "63\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [117] "64\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [118] "67\t2\t0.0\t4\t1 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [119] "68\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [120] "69\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [121] "70\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [122] "76\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [123] "78\t8\t0.0\t4\t6 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [124] "79\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [125] "81\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [126] "84\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [127] "85\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [128] "86\t47\t0.0\t4\t47"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [129] "87\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [130] "88\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [131] "89\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [132] "90\t8\t0.0\t4\t8"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [133] "92\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [134] "93\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [135] "94\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [136] "95\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [137] "100\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [138] "103\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [139] "104\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [140] "108\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [141] "110\t12\t0.0\t4\t11 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [142] "111\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [143] "114\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [144] "118\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [145] "119\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [146] "120\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [147] "122\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [148] "128\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [149] "131\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [150] "132\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [151] "137\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [152] "138\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [153] "140\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [154] "142\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [155] "145\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [156] "148\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [157] "153\t3\t0.0\t4\t0 0 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [158] "161\t9\t0.0\t4\t7 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [159] "174\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [160] "183\t6\t0.0\t4\t0 0 3 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [161] "184\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [162] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [163] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [164] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [165] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [166] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## 
## [[95]]
##  [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/GT31_S95_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/GT31_S95_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/GT31_S95_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/GT31_S95_L001_R2_001.fastq.gz"
##  [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [4] "Finished in 0.02 s (214 us/read; 0.28 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [8] "Total read pairs processed:                 95"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [9] "  Read 1 with adapter:                       0 (0.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [10] "  Read 2 with adapter:                       1 (1.1%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [11] "Pairs written (passing filters):            95 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [13] "Total basepairs processed:        39,900 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [14] "  Read 1:        18,620 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [15] "  Read 2:        21,280 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [16] "Total written (filtered):         39,857 bp (99.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [17] "  Read 1:        18,620 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [18] "  Read 2:        21,237 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [24] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [25] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [26] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [27] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [28] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [29] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [30] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [32] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [34] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [36] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [37] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [39] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [40] "  A: 100.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [41] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [42] "  G: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [43] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [44] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [45] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [46] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [47] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [48] "43\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## 
## [[96]]
##  [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/GT32_S96_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/GT32_S96_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/GT32_S96_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/GT32_S96_L001_R2_001.fastq.gz"
##  [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [4] "Finished in 0.48 s (118 us/read; 0.51 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [8] "Total read pairs processed:              4,025"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [9] "  Read 1 with adapter:                       1 (0.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [10] "  Read 2 with adapter:                      34 (0.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [11] "Pairs written (passing filters):         4,025 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [13] "Total basepairs processed:     1,690,500 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [14] "  Read 1:       788,900 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [15] "  Read 2:       901,600 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [16] "Total written (filtered):      1,688,318 bp (99.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [17] "  Read 1:       788,876 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [18] "  Read 2:       899,442 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [29] "24\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [30] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [32] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [34] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [36] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [38] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [39] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [40] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [41] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [42] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 34 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [43] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [44] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [45] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [46] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [47] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [48] "  A: 94.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [49] "  C: 2.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [50] "  G: 2.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [51] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [52] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [53] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [54] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [55] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [56] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [57] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [58] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [59] "5\t2\t3.9\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [60] "7\t1\t0.2\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [61] "9\t1\t0.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [62] "22\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [63] "23\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [64] "25\t2\t0.0\t2\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [65] "29\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [66] "34\t1\t0.0\t3\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [67] "43\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [68] "46\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [69] "50\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [70] "57\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [71] "60\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [72] "61\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [73] "64\t2\t0.0\t4\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [74] "71\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [75] "72\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [76] "73\t2\t0.0\t4\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [77] "78\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [78] "84\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [79] "95\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [80] "96\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [81] "98\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [82] "106\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [83] "109\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [84] "128\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [85] "145\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [86] "146\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [87] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [88] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [89] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [90] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [91] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## 
## [[97]]
##  [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/GT33_S97_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/GT33_S97_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/GT33_S97_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/GT33_S97_L001_R2_001.fastq.gz"
##  [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [4] "Finished in 0.11 s (117 us/read; 0.51 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [8] "Total read pairs processed:                964"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [9] "  Read 1 with adapter:                       0 (0.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [10] "  Read 2 with adapter:                      14 (1.5%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [11] "Pairs written (passing filters):           964 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [13] "Total basepairs processed:       404,880 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [14] "  Read 1:       188,944 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [15] "  Read 2:       215,936 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [16] "Total written (filtered):        404,240 bp (99.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [17] "  Read 1:       188,944 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [18] "  Read 2:       215,296 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [24] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [25] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [26] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [27] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [28] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [29] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [30] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [32] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [34] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 14 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [36] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [37] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [39] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [40] "  A: 92.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [41] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [42] "  G: 7.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [43] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [44] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [45] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [46] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [47] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [48] "5\t1\t0.9\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [49] "7\t1\t0.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [50] "8\t1\t0.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [51] "20\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [52] "25\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [53] "35\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [54] "39\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [55] "62\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [56] "66\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [57] "68\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [58] "72\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [59] "76\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [60] "85\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## 
## [[98]]
##  [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/GT3Cont1_S101_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/GT3Cont1_S101_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/GT3Cont1_S101_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/GT3Cont1_S101_L001_R2_001.fastq.gz"
##  [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [4] "Finished in 0.03 s (188 us/read; 0.32 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [8] "Total read pairs processed:                138"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [9] "  Read 1 with adapter:                       0 (0.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [10] "  Read 2 with adapter:                       7 (5.1%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [11] "Pairs written (passing filters):           138 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [13] "Total basepairs processed:        57,960 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [14] "  Read 1:        27,048 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [15] "  Read 2:        30,912 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [16] "Total written (filtered):         57,371 bp (99.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [17] "  Read 1:        27,048 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [18] "  Read 2:        30,323 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [24] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [25] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [26] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [27] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [28] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [29] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [30] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [32] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [34] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 7 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [36] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [37] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [39] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [40] "  A: 71.4%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [41] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [42] "  G: 28.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [43] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [44] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [45] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [46] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [47] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [48] "31\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [49] "35\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [50] "66\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [51] "92\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [52] "112\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [53] "125\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [54] "128\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## 
## [[99]]
##  [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/GT3Cont2_S102_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/GT3Cont2_S102_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/GT3Cont2_S102_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/GT3Cont2_S102_L001_R2_001.fastq.gz"
##  [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [4] "Finished in 0.07 s (150 us/read; 0.40 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [8] "Total read pairs processed:                468"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [9] "  Read 1 with adapter:                       0 (0.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [10] "  Read 2 with adapter:                      19 (4.1%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [11] "Pairs written (passing filters):           468 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [13] "Total basepairs processed:       196,560 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [14] "  Read 1:        91,728 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [15] "  Read 2:       104,832 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [16] "Total written (filtered):        195,082 bp (99.2%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [17] "  Read 1:        91,728 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [18] "  Read 2:       103,354 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [24] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [25] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [26] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [27] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [28] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [29] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [30] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [32] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [34] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 19 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [36] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [37] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [39] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [40] "  A: 89.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [41] "  C: 5.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [42] "  G: 5.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [43] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [44] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [45] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [46] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [47] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [48] "8\t1\t0.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [49] "20\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [50] "30\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [51] "55\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [52] "63\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [53] "66\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [54] "68\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [55] "70\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [56] "72\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [57] "78\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [58] "93\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [59] "105\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [60] "106\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [61] "130\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [62] "140\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [63] "164\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## 
## [[100]]
##  [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/GT3Cont3_S103_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/GT3Cont3_S103_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/GT3Cont3_S103_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/GT3Cont3_S103_L001_R2_001.fastq.gz"
##  [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [4] "Finished in 0.05 s (149 us/read; 0.40 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [8] "Total read pairs processed:                347"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [9] "  Read 1 with adapter:                       1 (0.3%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [10] "  Read 2 with adapter:                      10 (2.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [11] "Pairs written (passing filters):           347 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [13] "Total basepairs processed:       145,740 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [14] "  Read 1:        68,012 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [15] "  Read 2:        77,728 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [16] "Total written (filtered):        145,009 bp (99.5%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [17] "  Read 1:        67,993 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [18] "  Read 2:        77,016 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [29] "19\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [30] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [32] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [34] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [36] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [38] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [39] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [40] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [41] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [42] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 10 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [43] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [44] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [45] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [46] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [47] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [48] "  A: 60.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [49] "  C: 10.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [50] "  G: 30.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [51] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [52] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [53] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [54] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [55] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [56] "14\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [57] "15\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [58] "46\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [59] "51\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [60] "67\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [61] "82\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [62] "85\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [63] "97\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [64] "116\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [65] "139\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## 
## [[101]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/GT3ContRNA1_S104_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/GT3ContRNA1_S104_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/GT3ContRNA1_S104_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/GT3ContRNA1_S104_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##   [4] "Finished in 0.10 s (148 us/read; 0.41 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [8] "Total read pairs processed:                678"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [9] "  Read 1 with adapter:                       6 (0.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [10] "  Read 2 with adapter:                      26 (3.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [11] "Pairs written (passing filters):           677 (99.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [13] "Total basepairs processed:       284,760 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [14] "  Read 1:       132,888 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [15] "  Read 2:       151,872 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [16] "Total written (filtered):        282,200 bp (99.1%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [17] "  Read 1:       132,389 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [18] "  Read 2:       149,811 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 4 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [29] "6\t1\t0.2\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [30] "7\t1\t0.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [31] "8\t1\t0.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [32] "57\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [34] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [35] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [36] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [37] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 2 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [39] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [40] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [41] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [42] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [43] "  A: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [44] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [45] "  G: 100.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [46] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [47] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [48] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [49] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [50] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [51] "141\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [52] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [53] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [54] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [55] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [56] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [57] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [58] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [59] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [60] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 26 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [61] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [62] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [63] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [64] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [65] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [66] "  A: 84.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [67] "  C: 3.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [68] "  G: 11.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [69] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [70] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [71] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [72] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [73] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [74] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [75] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [76] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [77] "20\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [78] "26\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [79] "30\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [80] "33\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [81] "45\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [82] "48\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [83] "51\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [84] "52\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [85] "57\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [86] "58\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [87] "63\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [88] "67\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [89] "79\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [90] "85\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [91] "86\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [92] "89\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [93] "92\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [94] "115\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [95] "125\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [96] "128\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [97] "147\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [98] "156\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [99] "200\t1\t0.0\t4\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [100] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [101] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [102] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [103] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [104] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## 
## [[102]]
##  [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/GT3ContRNA2_S105_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/GT3ContRNA2_S105_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/GT3ContRNA2_S105_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/GT3ContRNA2_S105_L001_R2_001.fastq.gz"
##  [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [4] "Finished in 0.03 s (211 us/read; 0.28 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [8] "Total read pairs processed:                122"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [9] "  Read 1 with adapter:                       0 (0.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [10] "  Read 2 with adapter:                      11 (9.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [11] "Pairs written (passing filters):           122 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [13] "Total basepairs processed:        51,240 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [14] "  Read 1:        23,912 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [15] "  Read 2:        27,328 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [16] "Total written (filtered):         50,443 bp (98.4%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [17] "  Read 1:        23,912 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [18] "  Read 2:        26,531 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [24] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [25] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [26] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [27] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [28] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [29] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [30] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [32] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [34] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 11 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [36] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [37] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [39] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [40] "  A: 81.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [41] "  C: 9.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [42] "  G: 9.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [43] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [44] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [45] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [46] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [47] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [48] "19\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [49] "30\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [50] "36\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [51] "51\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [52] "78\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [53] "86\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [54] "95\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [55] "115\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## 
## [[103]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/GT3ContRNA3_S106_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/GT3ContRNA3_S106_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/GT3ContRNA3_S106_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/GT3ContRNA3_S106_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##   [4] "Finished in 1.07 s (114 us/read; 0.53 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [8] "Total read pairs processed:              9,352"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [9] "  Read 1 with adapter:                       2 (0.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [10] "  Read 2 with adapter:                     189 (2.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [11] "Pairs written (passing filters):         9,352 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [13] "Total basepairs processed:     3,927,840 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [14] "  Read 1:     1,832,992 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [15] "  Read 2:     2,094,848 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [16] "Total written (filtered):      3,915,534 bp (99.7%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [17] "  Read 1:     1,832,835 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [18] "  Read 2:     2,082,699 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [24] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [25] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [26] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 2 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [27] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [28] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [29] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [30] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [31] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [32] "  A: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [33] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [34] "  G: 50.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [35] "  T: 50.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [36] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [38] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [39] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [40] "75\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [41] "82\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [42] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [43] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [44] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [45] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [46] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [47] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [48] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [49] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [50] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 189 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [51] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [52] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [53] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [54] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [55] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [56] "  A: 58.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [57] "  C: 31.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [58] "  G: 2.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [59] "  T: 7.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [60] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [61] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [62] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [63] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [64] "5\t7\t9.1\t0\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [65] "6\t1\t2.3\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [66] "7\t4\t0.6\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [67] "9\t4\t0.0\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [68] "11\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [69] "12\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [70] "13\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [71] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [72] "18\t2\t0.0\t1\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [73] "19\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [74] "21\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [75] "22\t2\t0.0\t2\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [76] "24\t3\t0.0\t2\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [77] "25\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [78] "26\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [79] "30\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [80] "31\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [81] "32\t7\t0.0\t3\t5 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [82] "33\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [83] "34\t6\t0.0\t3\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [84] "35\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [85] "36\t1\t0.0\t3\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [86] "38\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [87] "39\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [88] "40\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [89] "41\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [90] "42\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [91] "43\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [92] "44\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [93] "45\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [94] "46\t2\t0.0\t4\t1 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [95] "47\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [96] "50\t2\t0.0\t4\t1 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [97] "51\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [98] "52\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [99] "53\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [100] "54\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [101] "56\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [102] "57\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [103] "58\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [104] "60\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [105] "61\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [106] "62\t2\t0.0\t4\t0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [107] "63\t7\t0.0\t4\t5 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [108] "64\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [109] "67\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [110] "69\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [111] "72\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [112] "74\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [113] "75\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [114] "76\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [115] "77\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [116] "78\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [117] "79\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [118] "81\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [119] "82\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [120] "83\t3\t0.0\t4\t1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [121] "84\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [122] "85\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [123] "86\t7\t0.0\t4\t5 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [124] "87\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [125] "88\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [126] "91\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [127] "92\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [128] "93\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [129] "94\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [130] "96\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [131] "98\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [132] "103\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [133] "105\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [134] "109\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [135] "110\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [136] "112\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [137] "113\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [138] "114\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [139] "115\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [140] "116\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [141] "117\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [142] "119\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [143] "120\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [144] "122\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [145] "123\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [146] "125\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [147] "126\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [148] "128\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [149] "129\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [150] "130\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [151] "132\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [152] "135\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [153] "142\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [154] "150\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## 
## [[104]]
##  [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/GT3RNA1_S98_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/GT3RNA1_S98_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/GT3RNA1_S98_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/GT3RNA1_S98_L001_R2_001.fastq.gz"
##  [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [4] "Finished in 0.02 s (201 us/read; 0.30 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [8] "Total read pairs processed:                109"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [9] "  Read 1 with adapter:                       1 (0.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [10] "  Read 2 with adapter:                       7 (6.4%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [11] "Pairs written (passing filters):           109 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [13] "Total basepairs processed:        45,780 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [14] "  Read 1:        21,364 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [15] "  Read 2:        24,416 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [16] "Total written (filtered):         45,349 bp (99.1%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [17] "  Read 1:        21,330 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [18] "  Read 2:        24,019 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [29] "34\t1\t0.0\t3\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [30] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [32] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [34] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [36] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [38] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [39] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [40] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [41] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [42] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 7 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [43] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [44] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [45] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [46] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [47] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [48] "  A: 42.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [49] "  C: 14.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [50] "  G: 42.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [51] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [52] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [53] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [54] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [55] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [56] "25\t1\t0.0\t2\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [57] "27\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [58] "30\t1\t0.0\t3\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [59] "60\t2\t0.0\t4\t0 1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [60] "75\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [61] "120\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## 
## [[105]]
##  [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/GT3RNA2_S99_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/GT3RNA2_S99_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/GT3RNA2_S99_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/GT3RNA2_S99_L001_R2_001.fastq.gz"
##  [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [4] "Finished in 0.10 s (137 us/read; 0.44 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [8] "Total read pairs processed:                695"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [9] "  Read 1 with adapter:                       1 (0.1%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [10] "  Read 2 with adapter:                      25 (3.6%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [11] "Pairs written (passing filters):           695 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [13] "Total basepairs processed:       291,900 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [14] "  Read 1:       136,220 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [15] "  Read 2:       155,680 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [16] "Total written (filtered):        290,340 bp (99.5%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [17] "  Read 1:       136,145 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [18] "  Read 2:       154,195 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [24] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [25] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [26] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [27] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [28] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [29] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [30] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [31] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [32] "  A: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [33] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [34] "  G: 100.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [35] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [36] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [38] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [39] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [40] "75\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [41] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [42] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [43] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [44] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [45] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [46] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [47] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [48] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [49] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 25 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [50] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [51] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [52] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [53] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [54] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [55] "  A: 88.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [56] "  C: 4.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [57] "  G: 8.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [58] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [59] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [60] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [61] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [62] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [63] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [64] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [65] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [66] "16\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [67] "17\t3\t0.0\t1\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [68] "22\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [69] "34\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [70] "42\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [71] "43\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [72] "46\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [73] "47\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [74] "51\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [75] "52\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [76] "55\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [77] "66\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [78] "69\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [79] "75\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [80] "85\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [81] "86\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [82] "94\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [83] "103\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [84] "127\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [85] "128\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [86] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [87] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [88] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [89] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [90] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## 
## [[106]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/GT3RNA3_S100_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/GT3RNA3_S100_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/GT3RNA3_S100_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/GT3RNA3_S100_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##   [4] "Finished in 0.20 s (134 us/read; 0.45 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [8] "Total read pairs processed:              1,531"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [9] "  Read 1 with adapter:                      19 (1.2%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [10] "  Read 2 with adapter:                      72 (4.7%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [11] "Pairs written (passing filters):         1,531 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [13] "Total basepairs processed:       643,020 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [14] "  Read 1:       300,076 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [15] "  Read 2:       342,944 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [16] "Total written (filtered):        635,297 bp (98.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [17] "  Read 1:       298,472 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [18] "  Read 2:       336,825 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [29] "20\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [30] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [32] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [34] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 18 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [36] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [37] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [39] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [40] "  A: 16.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [41] "  C: 33.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [42] "  G: 44.4%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [43] "  T: 5.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [44] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [45] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [46] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [47] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [48] "24\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [49] "71\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [50] "74\t4\t0.0\t2\t3 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [51] "82\t7\t0.0\t2\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [52] "88\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [53] "123\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [54] "125\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [55] "141\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [56] "142\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [57] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [58] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [59] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [60] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [61] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [62] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [63] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [64] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [65] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 72 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [66] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [67] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [68] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [69] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [70] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [71] "  A: 86.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [72] "  C: 2.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [73] "  G: 9.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [74] "  T: 1.4%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [75] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [76] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [77] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [78] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [79] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [80] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [81] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [82] "9\t4\t0.0\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [83] "10\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [84] "11\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [85] "16\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [86] "18\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [87] "19\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [88] "34\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [89] "39\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [90] "44\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [91] "45\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [92] "52\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [93] "53\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [94] "58\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [95] "59\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [96] "61\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [97] "63\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [98] "64\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [99] "76\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [100] "77\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [101] "79\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [102] "80\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [103] "81\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [104] "85\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [105] "86\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [106] "87\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [107] "91\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [108] "94\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [109] "95\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [110] "96\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [111] "97\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [112] "99\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [113] "102\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [114] "104\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [115] "105\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [116] "108\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [117] "109\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [118] "110\t7\t0.0\t4\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [119] "116\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [120] "119\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [121] "123\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [122] "129\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [123] "141\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [124] "144\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [125] "146\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [126] "153\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [127] "160\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [128] "169\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [129] "170\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [130] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [131] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [132] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [133] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [134] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## 
## [[107]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/InhibC_S107_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/InhibC_S107_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/InhibC_S107_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/InhibC_S107_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##   [4] "Finished in 1.05 s (112 us/read; 0.54 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [8] "Total read pairs processed:              9,436"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##   [9] "  Read 1 with adapter:                       5 (0.1%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [10] "  Read 2 with adapter:                     219 (2.3%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [11] "Pairs written (passing filters):         9,436 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [13] "Total basepairs processed:     3,963,120 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [14] "  Read 1:     1,849,456 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [15] "  Read 2:     2,113,664 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [16] "Total written (filtered):      3,948,319 bp (99.6%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [17] "  Read 1:     1,849,374 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [18] "  Read 2:     2,098,945 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 5 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [29] "5\t1\t9.2\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [30] "8\t1\t0.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [31] "20\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [32] "22\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [33] "27\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [34] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [36] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [38] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [39] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [40] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [41] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [42] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [43] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [44] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [45] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [46] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [47] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [48] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [49] "5\t1\t9.2\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [50] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [51] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [52] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [53] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [54] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 219 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [55] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [56] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [57] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [58] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [59] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [60] "  A: 98.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [61] "  C: 0.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [62] "  G: 0.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [63] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [64] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [65] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [66] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [67] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [68] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [69] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [70] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [71] "5\t4\t9.2\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [72] "6\t4\t2.3\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [73] "7\t1\t0.6\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [74] "8\t2\t0.1\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [75] "9\t1\t0.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [76] "10\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [77] "11\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [78] "12\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [79] "13\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [80] "14\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [81] "16\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [82] "17\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [83] "18\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [84] "19\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [85] "21\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [86] "22\t4\t0.0\t2\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [87] "25\t2\t0.0\t2\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [88] "27\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [89] "28\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [90] "29\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [91] "30\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [92] "31\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [93] "33\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [94] "34\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [95] "35\t3\t0.0\t3\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [96] "39\t3\t0.0\t3\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [97] "40\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [98] "41\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [99] "42\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [100] "43\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [101] "45\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [102] "46\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [103] "47\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [104] "48\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [105] "49\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [106] "50\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [107] "51\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [108] "52\t7\t0.0\t4\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [109] "53\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [110] "54\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [111] "55\t7\t0.0\t4\t6 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [112] "56\t3\t0.0\t4\t1 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [113] "57\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [114] "58\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [115] "59\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [116] "60\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [117] "62\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [118] "63\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [119] "64\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [120] "66\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [121] "67\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [122] "68\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [123] "71\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [124] "72\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [125] "73\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [126] "74\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [127] "75\t9\t0.0\t4\t9"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [128] "76\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [129] "77\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [130] "79\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [131] "80\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [132] "81\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [133] "82\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [134] "83\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [135] "84\t3\t0.0\t4\t2 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [136] "85\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [137] "86\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [138] "88\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [139] "89\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [140] "90\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [141] "91\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [142] "92\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [143] "93\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [144] "94\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [145] "95\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [146] "97\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [147] "98\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [148] "99\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [149] "101\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [150] "103\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [151] "105\t8\t0.0\t4\t8"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [152] "106\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [153] "107\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [154] "108\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [155] "110\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [156] "111\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [157] "112\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [158] "113\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [159] "114\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [160] "115\t3\t0.0\t4\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [161] "122\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [162] "128\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [163] "129\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [164] "131\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [165] "132\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [166] "135\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [167] "137\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [168] "141\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [169] "143\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [170] "146\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [171] "147\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [172] "150\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [173] "154\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [174] "160\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [175] "165\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [176] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [177] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [178] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [179] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [180] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## 
## [[108]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/MmixC_S108_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/MmixC_S108_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/MmixC_S108_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/MmixC_S108_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##   [4] "Finished in 0.52 s (123 us/read; 0.49 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##   [8] "Total read pairs processed:              4,260"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [9] "  Read 1 with adapter:                     181 (4.2%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [10] "  Read 2 with adapter:                     225 (5.3%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [11] "Pairs written (passing filters):         4,260 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [13] "Total basepairs processed:     1,789,200 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [14] "  Read 1:       834,960 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [15] "  Read 2:       954,240 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [16] "Total written (filtered):      1,753,961 bp (98.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [17] "  Read 1:       821,329 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [18] "  Read 2:       932,632 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [24] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [25] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [26] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 181 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [27] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [28] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [29] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [30] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [31] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [32] "  A: 29.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [33] "  C: 37.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [34] "  G: 12.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [35] "  T: 21.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [36] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [38] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [39] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [40] "16\t19\t0.0\t1\t19"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [41] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [42] "19\t12\t0.0\t1\t12"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [43] "62\t10\t0.0\t2\t9 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [44] "77\t39\t0.0\t2\t39"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [45] "80\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [46] "82\t65\t0.0\t2\t64 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [47] "83\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [48] "90\t11\t0.0\t2\t11"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [49] "135\t20\t0.0\t2\t18 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [50] "136\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [51] "140\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [52] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [53] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [54] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [55] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [56] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [57] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [58] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [59] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [60] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 225 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [61] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [62] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [63] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [64] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [65] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [66] "  A: 98.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [67] "  C: 0.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [68] "  G: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [69] "  T: 0.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [70] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [71] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [72] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [73] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [74] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [75] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [76] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [77] "6\t1\t1.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [78] "8\t1\t0.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [79] "13\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [80] "15\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [81] "16\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [82] "24\t9\t0.0\t2\t5 2 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [83] "27\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [84] "33\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [85] "35\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [86] "38\t3\t0.0\t3\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [87] "42\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [88] "44\t15\t0.0\t4\t6 6 1 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [89] "45\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [90] "47\t9\t0.0\t4\t2 2 4 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [91] "48\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [92] "52\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [93] "55\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [94] "56\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [95] "58\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [96] "60\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [97] "61\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [98] "66\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [99] "69\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [100] "79\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [101] "82\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [102] "85\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [103] "87\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [104] "90\t10\t0.0\t4\t9 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [105] "91\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [106] "94\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [107] "103\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [108] "105\t39\t0.0\t4\t38 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [109] "107\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [110] "110\t66\t0.0\t4\t58 7 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [111] "112\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [112] "116\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [113] "117\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [114] "118\t11\t0.0\t4\t11"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [115] "119\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [116] "121\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [117] "122\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [118] "130\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [119] "136\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [120] "142\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [121] "143\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [122] "145\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [123] "151\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [124] "163\t20\t0.0\t4\t18 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [125] "167\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [126] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [127] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [128] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [129] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [130] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## 
## [[109]]
##  [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/NPCRC_S109_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/NPCRC_S109_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/NPCRC_S109_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/NPCRC_S109_L001_R2_001.fastq.gz"
##  [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [4] "Finished in 0.03 s (171 us/read; 0.35 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [8] "Total read pairs processed:                196"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [9] "  Read 1 with adapter:                      34 (17.3%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [10] "  Read 2 with adapter:                       2 (1.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [11] "Pairs written (passing filters):           195 (99.5%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [13] "Total basepairs processed:        82,320 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [14] "  Read 1:        38,416 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [15] "  Read 2:        43,904 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [16] "Total written (filtered):         77,392 bp (94.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [17] "  Read 1:        33,864 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [18] "  Read 2:        43,528 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [24] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [25] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [26] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 34 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [27] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [28] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [29] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [30] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [31] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [32] "  A: 5.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [33] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [34] "  G: 94.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [35] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [36] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [37] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [38] "    The adapter is preceded by \"G\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [39] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [40] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [41] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [42] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [43] "87\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [44] "88\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [45] "97\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [46] "137\t9\t0.0\t2\t9"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [47] "138\t7\t0.0\t2\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [48] "139\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [49] "141\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [50] "142\t6\t0.0\t2\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [51] "143\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [52] "144\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [53] "146\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [54] "147\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [55] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [56] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [57] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [58] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [59] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [60] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [61] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [62] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [63] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 2 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [64] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [65] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [66] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [67] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [68] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [69] "  A: 100.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [70] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [71] "  G: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [72] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [73] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [74] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [75] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [76] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [77] "43\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [78] "109\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [79] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [80] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [81] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [82] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [83] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## 
## [[110]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/PPCRC_S110_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/PPCRC_S110_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/PPCRC_S110_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/PPCRC_S110_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##   [4] "Finished in 0.79 s (106 us/read; 0.57 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##   [8] "Total read pairs processed:              7,423"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [9] "  Read 1 with adapter:                       2 (0.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [10] "  Read 2 with adapter:                     148 (2.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [11] "Pairs written (passing filters):         7,423 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [13] "Total basepairs processed:     3,117,660 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [14] "  Read 1:     1,454,908 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [15] "  Read 2:     1,662,752 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [16] "Total written (filtered):      3,108,842 bp (99.7%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [17] "  Read 1:     1,454,881 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [18] "  Read 2:     1,653,961 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 2 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [29] "10\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [30] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [32] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [33] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [34] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [35] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [36] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [37] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [39] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [40] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [41] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [42] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [43] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 148 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [44] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [45] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [46] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [47] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [48] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [49] "  A: 98.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [50] "  C: 2.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [51] "  G: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [52] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [53] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [54] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [55] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [56] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [57] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [58] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [59] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [60] "5\t3\t7.2\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [61] "6\t1\t1.8\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [62] "7\t1\t0.5\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [63] "8\t6\t0.1\t0\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [64] "10\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [65] "16\t5\t0.0\t1\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [66] "17\t4\t0.0\t1\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [67] "19\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [68] "20\t3\t0.0\t2\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [69] "21\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [70] "22\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [71] "24\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [72] "26\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [73] "27\t2\t0.0\t2\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [74] "30\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [75] "31\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [76] "33\t6\t0.0\t3\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [77] "35\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [78] "36\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [79] "38\t3\t0.0\t3\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [80] "40\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [81] "43\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [82] "45\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [83] "46\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [84] "47\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [85] "50\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [86] "51\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [87] "52\t4\t0.0\t4\t1 2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [88] "53\t2\t0.0\t4\t0 1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [89] "54\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [90] "55\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [91] "56\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [92] "57\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [93] "58\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [94] "59\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [95] "63\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [96] "64\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [97] "65\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [98] "72\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [99] "73\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [100] "74\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [101] "77\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [102] "78\t1\t0.0\t4\t0 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [103] "79\t2\t0.0\t4\t1 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [104] "80\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [105] "82\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [106] "83\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [107] "85\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [108] "86\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [109] "88\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [110] "89\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [111] "94\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [112] "95\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [113] "96\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [114] "98\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [115] "99\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [116] "105\t5\t0.0\t4\t3 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [117] "106\t3\t0.0\t4\t2 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [118] "108\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [119] "111\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [120] "112\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [121] "118\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [122] "120\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [123] "121\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [124] "134\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [125] "145\t1\t0.0\t4\t0 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [126] "150\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [127] "153\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [128] "155\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [129] "157\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [130] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [131] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [132] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [133] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [134] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## 
## [[111]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/QB1_S111_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/QB1_S111_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/QB1_S111_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/QB1_S111_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##   [4] "Finished in 1.64 s (119 us/read; 0.50 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [8] "Total read pairs processed:             13,764"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [9] "  Read 1 with adapter:                       3 (0.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [10] "  Read 2 with adapter:                     122 (0.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [11] "Pairs written (passing filters):        13,764 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [13] "Total basepairs processed:     5,780,880 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [14] "  Read 1:     2,697,744 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [15] "  Read 2:     3,083,136 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [16] "Total written (filtered):      5,773,103 bp (99.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [17] "  Read 1:     2,697,643 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [18] "  Read 2:     3,075,460 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 2 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [29] "20\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [30] "35\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [32] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [33] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [34] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [35] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [36] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [37] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [38] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [39] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [40] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [41] "  A: 100.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [42] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [43] "  G: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [44] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [45] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [46] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [47] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [48] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [49] "46\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [50] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [51] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [52] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [53] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [54] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [55] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [56] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [57] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [58] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 122 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [59] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [60] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [61] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [62] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [63] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [64] "  A: 69.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [65] "  C: 26.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [66] "  G: 0.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [67] "  T: 3.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [68] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [69] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [70] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [71] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [72] "5\t1\t13.4\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [73] "6\t1\t3.4\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [74] "7\t1\t0.8\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [75] "9\t2\t0.1\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [76] "10\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [77] "12\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [78] "13\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [79] "14\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [80] "16\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [81] "17\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [82] "18\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [83] "20\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [84] "21\t3\t0.0\t2\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [85] "23\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [86] "24\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [87] "26\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [88] "27\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [89] "28\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [90] "33\t3\t0.0\t3\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [91] "34\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [92] "35\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [93] "36\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [94] "38\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [95] "39\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [96] "41\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [97] "43\t2\t0.0\t4\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [98] "45\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [99] "46\t3\t0.0\t4\t1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [100] "48\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [101] "50\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [102] "53\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [103] "54\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [104] "55\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [105] "58\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [106] "61\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [107] "62\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [108] "63\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [109] "64\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [110] "65\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [111] "66\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [112] "72\t7\t0.0\t4\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [113] "74\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [114] "76\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [115] "79\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [116] "80\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [117] "81\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [118] "82\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [119] "84\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [120] "85\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [121] "86\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [122] "88\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [123] "90\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [124] "91\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [125] "92\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [126] "93\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [127] "94\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [128] "98\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [129] "99\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [130] "100\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [131] "102\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [132] "104\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [133] "105\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [134] "109\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [135] "111\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [136] "115\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [137] "121\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [138] "129\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [139] "130\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [140] "133\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [141] "137\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [142] "143\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [143] "154\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [144] "157\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [145] "165\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## 
## [[112]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/QB2_S112_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/QB2_S112_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/QB2_S112_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/QB2_S112_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##   [4] "Finished in 2.39 s (117 us/read; 0.51 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [8] "Total read pairs processed:             20,333"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [9] "  Read 1 with adapter:                       6 (0.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [10] "  Read 2 with adapter:                     149 (0.7%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [11] "Pairs written (passing filters):        20,333 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [13] "Total basepairs processed:     8,539,860 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [14] "  Read 1:     3,985,268 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [15] "  Read 2:     4,554,592 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [16] "Total written (filtered):      8,530,728 bp (99.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [17] "  Read 1:     3,985,136 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [18] "  Read 2:     4,545,592 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 4 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [29] "6\t3\t5.0\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [30] "14\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [32] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [33] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [34] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [35] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 2 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [36] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [37] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [38] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [39] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [40] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [41] "  A: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [42] "  C: 50.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [43] "  G: 50.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [44] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [45] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [46] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [47] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [48] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [49] "10\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [50] "90\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [51] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [52] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [53] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [54] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [55] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [56] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [57] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [58] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [59] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [60] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [61] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [62] "5\t1\t19.9\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [63] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [64] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [65] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [66] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [67] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 148 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [68] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [69] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [70] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [71] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [72] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [73] "  A: 81.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [74] "  C: 12.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [75] "  G: 2.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [76] "  T: 4.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [77] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [78] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [79] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [80] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [81] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [82] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [83] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [84] "5\t4\t19.9\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [85] "6\t1\t5.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [86] "7\t3\t1.2\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [87] "8\t2\t0.3\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [88] "9\t2\t0.1\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [89] "10\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [90] "12\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [91] "13\t4\t0.0\t1\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [92] "14\t4\t0.0\t1\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [93] "15\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [94] "16\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [95] "17\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [96] "19\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [97] "20\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [98] "21\t4\t0.0\t2\t1 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [99] "23\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [100] "26\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [101] "28\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [102] "29\t4\t0.0\t2\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [103] "31\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [104] "32\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [105] "33\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [106] "34\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [107] "35\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [108] "36\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [109] "38\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [110] "40\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [111] "42\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [112] "43\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [113] "44\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [114] "45\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [115] "46\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [116] "47\t2\t0.0\t4\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [117] "51\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [118] "52\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [119] "53\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [120] "54\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [121] "57\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [122] "59\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [123] "61\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [124] "62\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [125] "63\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [126] "65\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [127] "66\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [128] "68\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [129] "72\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [130] "74\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [131] "75\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [132] "76\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [133] "79\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [134] "81\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [135] "83\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [136] "84\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [137] "85\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [138] "86\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [139] "87\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [140] "89\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [141] "91\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [142] "92\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [143] "93\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [144] "95\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [145] "96\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [146] "97\t2\t0.0\t4\t0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [147] "99\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [148] "100\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [149] "102\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [150] "103\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [151] "105\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [152] "112\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [153] "114\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [154] "115\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [155] "118\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [156] "120\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [157] "121\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [158] "122\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [159] "130\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [160] "131\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [161] "132\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [162] "138\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [163] "140\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [164] "147\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [165] "148\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [166] "156\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [167] "160\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [168] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [169] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [170] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [171] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [172] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## 
## [[113]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/QB3_S113_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/QB3_S113_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/QB3_S113_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/QB3_S113_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##   [4] "Finished in 1.93 s (118 us/read; 0.51 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [8] "Total read pairs processed:             16,350"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [9] "  Read 1 with adapter:                       6 (0.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [10] "  Read 2 with adapter:                     178 (1.1%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [11] "Pairs written (passing filters):        16,350 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [13] "Total basepairs processed:     6,867,000 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [14] "  Read 1:     3,204,600 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [15] "  Read 2:     3,662,400 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [16] "Total written (filtered):      6,856,556 bp (99.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [17] "  Read 1:     3,204,507 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [18] "  Read 2:     3,652,049 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 5 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [29] "7\t2\t1.0\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [30] "13\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [31] "15\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [32] "18\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [34] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [35] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [36] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [37] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [39] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [40] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [41] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [42] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [43] "  A: 100.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [44] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [45] "  G: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [46] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [47] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [48] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [49] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [50] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [51] "33\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [52] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [53] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [54] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [55] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [56] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [57] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [58] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [59] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [60] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [61] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [62] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [63] "58\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [64] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [65] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [66] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [67] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [68] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 177 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [69] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [70] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [71] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [72] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [73] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [74] "  A: 61.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [75] "  C: 31.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [76] "  G: 3.4%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [77] "  T: 4.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [78] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [79] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [80] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [81] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [82] "5\t8\t16.0\t0\t8"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [83] "6\t2\t4.0\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [84] "7\t4\t1.0\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [85] "8\t1\t0.2\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [86] "9\t2\t0.1\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [87] "10\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [88] "12\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [89] "13\t4\t0.0\t1\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [90] "14\t7\t0.0\t1\t6 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [91] "15\t5\t0.0\t1\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [92] "16\t7\t0.0\t1\t6 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [93] "17\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [94] "18\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [95] "20\t4\t0.0\t2\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [96] "23\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [97] "24\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [98] "26\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [99] "28\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [100] "29\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [101] "30\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [102] "33\t3\t0.0\t3\t1 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [103] "35\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [104] "37\t1\t0.0\t3\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [105] "38\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [106] "40\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [107] "42\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [108] "45\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [109] "46\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [110] "47\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [111] "48\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [112] "50\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [113] "52\t5\t0.0\t4\t4 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [114] "53\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [115] "54\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [116] "55\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [117] "56\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [118] "58\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [119] "59\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [120] "60\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [121] "61\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [122] "62\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [123] "63\t8\t0.0\t4\t6 0 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [124] "64\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [125] "65\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [126] "66\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [127] "67\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [128] "71\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [129] "72\t5\t0.0\t4\t4 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [130] "73\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [131] "74\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [132] "75\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [133] "76\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [134] "80\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [135] "81\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [136] "82\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [137] "83\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [138] "84\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [139] "85\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [140] "87\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [141] "88\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [142] "90\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [143] "92\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [144] "94\t5\t0.0\t4\t3 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [145] "95\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [146] "96\t2\t0.0\t4\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [147] "98\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [148] "99\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [149] "102\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [150] "103\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [151] "105\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [152] "107\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [153] "109\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [154] "110\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [155] "111\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [156] "112\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [157] "117\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [158] "119\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [159] "122\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [160] "130\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [161] "133\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [162] "134\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [163] "136\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [164] "137\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [165] "140\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [166] "148\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [167] "150\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [168] "159\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## 
## [[114]]
##  [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/QBRNA1_S114_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/QBRNA1_S114_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/QBRNA1_S114_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/QBRNA1_S114_L001_R2_001.fastq.gz"
##  [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [4] "Finished in 0.20 s (123 us/read; 0.49 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [8] "Total read pairs processed:              1,634"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [9] "  Read 1 with adapter:                      29 (1.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [10] "  Read 2 with adapter:                      45 (2.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [11] "Pairs written (passing filters):         1,634 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [13] "Total basepairs processed:       686,280 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [14] "  Read 1:       320,264 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [15] "  Read 2:       366,016 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [16] "Total written (filtered):        680,897 bp (99.2%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [17] "  Read 1:       318,550 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [18] "  Read 2:       362,347 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [24] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [25] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [26] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 29 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [27] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [28] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [29] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [30] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [31] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [32] "  A: 6.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [33] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [34] "  G: 93.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [35] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [36] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [37] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [38] "    The adapter is preceded by \"G\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [39] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [40] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [41] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [42] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [43] "34\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [44] "36\t9\t0.0\t2\t7 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [45] "38\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [46] "61\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [47] "75\t14\t0.0\t2\t14"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [48] "135\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [49] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [50] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [51] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [52] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [53] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [54] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [55] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [56] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [57] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 45 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [58] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [59] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [60] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [61] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [62] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [63] "  A: 88.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [64] "  C: 2.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [65] "  G: 6.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [66] "  T: 2.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [67] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [68] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [69] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [70] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [71] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [72] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [73] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [74] "6\t1\t0.4\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [75] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [76] "37\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [77] "45\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [78] "48\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [79] "50\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [80] "52\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [81] "57\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [82] "64\t11\t0.0\t4\t7 1 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [83] "65\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [84] "69\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [85] "73\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [86] "89\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [87] "101\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [88] "102\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [89] "103\t14\t0.0\t4\t13 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [90] "106\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [91] "114\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [92] "127\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [93] "137\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [94] "163\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [95] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [96] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [97] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [98] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [99] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## 
## [[115]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/QBRNA2_S115_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/QBRNA2_S115_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/QBRNA2_S115_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/QBRNA2_S115_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##   [4] "Finished in 0.11 s (130 us/read; 0.46 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [8] "Total read pairs processed:                872"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##   [9] "  Read 1 with adapter:                      27 (3.1%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [10] "  Read 2 with adapter:                      38 (4.4%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [11] "Pairs written (passing filters):           872 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [13] "Total basepairs processed:       366,240 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [14] "  Read 1:       170,912 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [15] "  Read 2:       195,328 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [16] "Total written (filtered):        361,942 bp (98.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [17] "  Read 1:       169,308 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [18] "  Read 2:       192,634 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 2 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [29] "6\t1\t0.2\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [30] "31\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [32] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [33] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [34] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [35] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 25 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [36] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [37] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [38] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [39] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [40] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [41] "  A: 8.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [42] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [43] "  G: 92.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [44] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [45] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [46] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [47] "    The adapter is preceded by \"G\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [48] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [49] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [50] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [51] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [52] "15\t6\t0.0\t1\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [53] "50\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [54] "65\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [55] "75\t11\t0.0\t2\t11"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [56] "76\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [57] "139\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [58] "142\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [59] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [60] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [61] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [62] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [63] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [64] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [65] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [66] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [67] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 38 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [68] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [69] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [70] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [71] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [72] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [73] "  A: 71.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [74] "  C: 10.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [75] "  G: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [76] "  T: 18.4%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [77] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [78] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [79] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [80] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [81] "8\t2\t0.0\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [82] "13\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [83] "14\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [84] "20\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [85] "34\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [86] "37\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [87] "39\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [88] "43\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [89] "58\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [90] "63\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [91] "65\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [92] "68\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [93] "93\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [94] "98\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [95] "102\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [96] "103\t12\t0.0\t4\t10 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [97] "138\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [98] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [99] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [100] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [101] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [102] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## 
## [[116]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/QBRNA3_S116_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/QBRNA3_S116_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/QBRNA3_S116_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/QBRNA3_S116_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##   [4] "Finished in 0.09 s (142 us/read; 0.42 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [8] "Total read pairs processed:                645"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##   [9] "  Read 1 with adapter:                      13 (2.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [10] "  Read 2 with adapter:                      29 (4.5%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [11] "Pairs written (passing filters):           645 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [13] "Total basepairs processed:       270,900 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [14] "  Read 1:       126,420 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [15] "  Read 2:       144,480 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [16] "Total written (filtered):        267,545 bp (98.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [17] "  Read 1:       124,916 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [18] "  Read 2:       142,629 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 2 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [29] "6\t1\t0.2\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [30] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [32] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [33] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [34] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [35] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 11 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [36] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [37] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [38] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [39] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [40] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [41] "  A: 18.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [42] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [43] "  G: 81.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [44] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [45] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [46] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [47] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [48] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [49] "124\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [50] "128\t4\t0.0\t2\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [51] "137\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [52] "141\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [53] "142\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [54] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [55] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [56] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [57] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [58] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [59] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [60] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [61] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [62] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 29 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [63] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [64] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [65] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [66] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [67] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [68] "  A: 65.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [69] "  C: 3.4%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [70] "  G: 27.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [71] "  T: 3.4%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [72] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [73] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [74] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [75] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [76] "5\t1\t0.6\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [77] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [78] "22\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [79] "23\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [80] "33\t1\t0.0\t3\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [81] "43\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [82] "45\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [83] "47\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [84] "49\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [85] "55\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [86] "58\t2\t0.0\t4\t1 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [87] "59\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [88] "61\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [89] "63\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [90] "65\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [91] "67\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [92] "72\t1\t0.0\t4\t0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [93] "76\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [94] "78\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [95] "79\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [96] "83\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [97] "84\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [98] "88\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [99] "89\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [100] "105\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [101] "116\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [102] "127\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## 
## [[117]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/QW1_S117_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/QW1_S117_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/QW1_S117_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/QW1_S117_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##   [4] "Finished in 2.37 s (117 us/read; 0.51 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [8] "Total read pairs processed:             20,338"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [9] "  Read 1 with adapter:                      16 (0.1%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [10] "  Read 2 with adapter:                     174 (0.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [11] "Pairs written (passing filters):        20,337 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [13] "Total basepairs processed:     8,541,960 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [14] "  Read 1:     3,986,248 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [15] "  Read 2:     4,555,712 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [16] "Total written (filtered):      8,530,245 bp (99.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [17] "  Read 1:     3,985,724 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [18] "  Read 2:     4,544,521 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 5 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [29] "6\t1\t5.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [30] "7\t1\t1.2\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [31] "27\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [32] "31\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [33] "34\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [34] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [36] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [38] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 11 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [39] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [40] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [41] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [42] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [43] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [44] "  A: 9.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [45] "  C: 72.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [46] "  G: 18.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [47] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [48] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [49] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [50] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [51] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [52] "11\t6\t0.0\t1\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [53] "12\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [54] "15\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [55] "32\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [56] "66\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [57] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [58] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [59] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [60] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [61] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [62] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [63] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [64] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [65] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [66] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [67] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [68] "19\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [69] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [70] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [71] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [72] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [73] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 173 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [74] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [75] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [76] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [77] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [78] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [79] "  A: 83.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [80] "  C: 11.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [81] "  G: 1.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [82] "  T: 4.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [83] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [84] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [85] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [86] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [87] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [88] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [89] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [90] "5\t7\t19.9\t0\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [91] "6\t2\t5.0\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [92] "7\t4\t1.2\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [93] "8\t2\t0.3\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [94] "9\t2\t0.1\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [95] "10\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [96] "12\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [97] "13\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [98] "14\t5\t0.0\t1\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [99] "16\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [100] "17\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [101] "18\t3\t0.0\t1\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [102] "20\t3\t0.0\t2\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [103] "21\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [104] "22\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [105] "24\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [106] "27\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [107] "28\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [108] "29\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [109] "30\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [110] "31\t3\t0.0\t3\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [111] "32\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [112] "33\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [113] "35\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [114] "38\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [115] "39\t8\t0.0\t3\t4 2 1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [116] "40\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [117] "41\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [118] "42\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [119] "43\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [120] "44\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [121] "45\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [122] "46\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [123] "47\t3\t0.0\t4\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [124] "51\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [125] "55\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [126] "56\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [127] "58\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [128] "59\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [129] "60\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [130] "62\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [131] "64\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [132] "65\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [133] "68\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [134] "71\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [135] "72\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [136] "73\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [137] "74\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [138] "75\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [139] "76\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [140] "77\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [141] "79\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [142] "80\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [143] "82\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [144] "84\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [145] "85\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [146] "86\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [147] "87\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [148] "88\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [149] "89\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [150] "91\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [151] "92\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [152] "93\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [153] "94\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [154] "95\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [155] "98\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [156] "99\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [157] "100\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [158] "102\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [159] "103\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [160] "104\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [161] "105\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [162] "106\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [163] "108\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [164] "109\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [165] "114\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [166] "115\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [167] "122\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [168] "123\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [169] "125\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [170] "127\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [171] "129\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [172] "131\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [173] "133\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [174] "135\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [175] "138\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [176] "140\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [177] "146\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [178] "149\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [179] "153\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [180] "160\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [181] "176\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [182] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [183] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [184] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [185] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [186] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## 
## [[118]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/QW2_S118_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/QW2_S118_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/QW2_S118_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/QW2_S118_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##   [4] "Finished in 2.19 s (116 us/read; 0.52 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [8] "Total read pairs processed:             18,861"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [9] "  Read 1 with adapter:                      12 (0.1%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [10] "  Read 2 with adapter:                     178 (0.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [11] "Pairs written (passing filters):        18,861 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [13] "Total basepairs processed:     7,921,620 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [14] "  Read 1:     3,696,756 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [15] "  Read 2:     4,224,864 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [16] "Total written (filtered):      7,910,521 bp (99.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [17] "  Read 1:     3,696,489 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [18] "  Read 2:     4,214,032 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 10 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [29] "5\t3\t18.4\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [30] "7\t2\t1.2\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [31] "8\t1\t0.3\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [32] "12\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [33] "13\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [34] "21\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [35] "34\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [36] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [38] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [39] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [40] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 2 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [41] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [42] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [43] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [44] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [45] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [46] "  A: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [47] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [48] "  G: 100.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [49] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [50] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [51] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [52] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [53] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [54] "51\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [55] "99\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [56] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [57] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [58] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [59] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [60] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 3 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [61] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [62] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [63] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [64] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [65] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [66] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [67] "5\t1\t18.4\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [68] "6\t1\t4.6\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [69] "30\t1\t0.0\t2\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [70] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [71] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [72] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [73] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [74] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 176 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [75] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [76] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [77] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [78] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [79] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [80] "  A: 71.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [81] "  C: 25.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [82] "  G: 1.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [83] "  T: 1.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [84] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [85] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [86] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [87] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [88] "5\t9\t18.4\t0\t9"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [89] "6\t5\t4.6\t0\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [90] "7\t1\t1.2\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [91] "8\t5\t0.3\t0\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [92] "9\t1\t0.1\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [93] "10\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [94] "11\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [95] "12\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [96] "13\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [97] "14\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [98] "15\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [99] "16\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [100] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [101] "19\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [102] "20\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [103] "21\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [104] "22\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [105] "23\t2\t0.0\t2\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [106] "24\t2\t0.0\t2\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [107] "25\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [108] "26\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [109] "28\t2\t0.0\t2\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [110] "30\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [111] "32\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [112] "33\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [113] "34\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [114] "35\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [115] "36\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [116] "37\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [117] "38\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [118] "39\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [119] "40\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [120] "41\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [121] "42\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [122] "43\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [123] "46\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [124] "48\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [125] "49\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [126] "51\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [127] "52\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [128] "55\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [129] "56\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [130] "57\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [131] "61\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [132] "63\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [133] "64\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [134] "65\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [135] "67\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [136] "68\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [137] "69\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [138] "70\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [139] "71\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [140] "72\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [141] "73\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [142] "74\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [143] "75\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [144] "76\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [145] "77\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [146] "78\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [147] "81\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [148] "82\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [149] "84\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [150] "85\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [151] "86\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [152] "87\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [153] "89\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [154] "90\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [155] "91\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [156] "94\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [157] "98\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [158] "99\t6\t0.0\t4\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [159] "100\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [160] "101\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [161] "102\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [162] "103\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [163] "106\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [164] "108\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [165] "109\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [166] "110\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [167] "112\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [168] "119\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [169] "120\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [170] "127\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [171] "132\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [172] "133\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [173] "134\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [174] "136\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [175] "139\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [176] "141\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [177] "146\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [178] "153\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [179] "154\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [180] "155\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [181] "156\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## 
## [[119]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/QW3_S119_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/QW3_S119_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/QW3_S119_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/QW3_S119_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##   [4] "Finished in 1.67 s (116 us/read; 0.52 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [8] "Total read pairs processed:             14,379"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [9] "  Read 1 with adapter:                       3 (0.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [10] "  Read 2 with adapter:                     191 (1.3%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [11] "Pairs written (passing filters):        14,379 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [13] "Total basepairs processed:     6,039,180 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [14] "  Read 1:     2,818,284 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [15] "  Read 2:     3,220,896 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [16] "Total written (filtered):      6,027,121 bp (99.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [17] "  Read 1:     2,818,199 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [18] "  Read 2:     3,208,922 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 2 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [29] "5\t1\t14.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [30] "21\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [32] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [33] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [34] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [35] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [36] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [37] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [38] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [39] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [40] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [41] "  A: 100.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [42] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [43] "  G: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [44] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [45] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [46] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [47] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [48] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [49] "59\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [50] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [51] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [52] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [53] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [54] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 2 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [55] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [56] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [57] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [58] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [59] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [60] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [61] "5\t1\t14.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [62] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [63] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [64] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [65] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [66] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [67] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 190 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [68] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [69] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [70] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [71] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [72] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [73] "  A: 74.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [74] "  C: 18.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [75] "  G: 3.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [76] "  T: 3.2%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [77] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [78] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [79] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [80] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [81] "5\t11\t14.0\t0\t11"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [82] "6\t3\t3.5\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [83] "7\t1\t0.9\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [84] "8\t4\t0.2\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [85] "9\t4\t0.1\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [86] "10\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [87] "13\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [88] "14\t4\t0.0\t1\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [89] "15\t5\t0.0\t1\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [90] "16\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [91] "17\t2\t0.0\t1\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [92] "20\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [93] "21\t4\t0.0\t2\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [94] "22\t5\t0.0\t2\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [95] "24\t4\t0.0\t2\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [96] "27\t2\t0.0\t2\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [97] "31\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [98] "32\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [99] "33\t3\t0.0\t3\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [100] "34\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [101] "37\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [102] "39\t2\t0.0\t3\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [103] "42\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [104] "44\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [105] "45\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [106] "47\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [107] "50\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [108] "51\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [109] "52\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [110] "53\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [111] "55\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [112] "57\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [113] "58\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [114] "59\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [115] "61\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [116] "63\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [117] "64\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [118] "65\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [119] "70\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [120] "71\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [121] "72\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [122] "73\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [123] "74\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [124] "75\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [125] "76\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [126] "77\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [127] "78\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [128] "79\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [129] "80\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [130] "82\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [131] "84\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [132] "85\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [133] "86\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [134] "87\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [135] "91\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [136] "93\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [137] "94\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [138] "95\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [139] "96\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [140] "98\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [141] "99\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [142] "100\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [143] "101\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [144] "102\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [145] "105\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [146] "107\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [147] "109\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [148] "110\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [149] "112\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [150] "113\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [151] "115\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [152] "116\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [153] "118\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [154] "120\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [155] "126\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [156] "134\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [157] "135\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [158] "136\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [159] "138\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [160] "143\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [161] "144\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [162] "151\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [163] "152\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [164] "156\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [165] "159\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [166] "160\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [167] "164\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [168] "167\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## 
## [[120]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/QWRNA1_S120_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/QWRNA1_S120_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/QWRNA1_S120_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/QWRNA1_S120_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##   [4] "Finished in 1.44 s (120 us/read; 0.50 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [8] "Total read pairs processed:             12,030"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##   [9] "  Read 1 with adapter:                     354 (2.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [10] "  Read 2 with adapter:                     546 (4.5%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [11] "Pairs written (passing filters):        12,021 (99.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [13] "Total basepairs processed:     5,052,600 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [14] "  Read 1:     2,357,880 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [15] "  Read 2:     2,694,720 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [16] "Total written (filtered):      4,976,574 bp (98.5%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [17] "  Read 1:     2,330,570 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [18] "  Read 2:     2,646,004 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [24] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [25] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [26] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 354 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [27] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [28] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [29] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [30] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [31] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [32] "  A: 7.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [33] "  C: 6.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [34] "  G: 85.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [35] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [36] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [37] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [38] "    The adapter is preceded by \"G\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [39] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [40] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [41] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [42] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [43] "16\t8\t0.0\t1\t8"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [44] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [45] "26\t40\t0.0\t2\t39 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [46] "27\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [47] "62\t8\t0.0\t2\t7 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [48] "65\t13\t0.0\t2\t12 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [49] "66\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [50] "68\t28\t0.0\t2\t28"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [51] "69\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [52] "72\t8\t0.0\t2\t6 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [53] "73\t34\t0.0\t2\t34"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [54] "74\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [55] "75\t10\t0.0\t2\t10"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [56] "81\t13\t0.0\t2\t13"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [57] "82\t11\t0.0\t2\t10 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [58] "87\t14\t0.0\t2\t14"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [59] "90\t137\t0.0\t2\t134 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [60] "91\t9\t0.0\t2\t9"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [61] "117\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [62] "141\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [63] "146\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [64] "148\t6\t0.0\t2\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [65] "157\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [66] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [67] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [68] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [69] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [70] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [71] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [72] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [73] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [74] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [75] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [76] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [77] "56\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [78] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [79] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [80] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [81] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [82] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 545 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [83] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [84] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [85] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [86] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [87] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [88] "  A: 81.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [89] "  C: 13.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [90] "  G: 1.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [91] "  T: 3.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [92] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [93] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [94] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [95] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [96] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [97] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [98] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [99] "5\t4\t11.7\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [100] "6\t1\t2.9\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [101] "7\t2\t0.7\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [102] "8\t1\t0.2\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [103] "11\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [104] "12\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [105] "15\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [106] "16\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [107] "17\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [108] "18\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [109] "20\t5\t0.0\t2\t4 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [110] "22\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [111] "24\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [112] "25\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [113] "26\t1\t0.0\t2\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [114] "27\t45\t0.0\t2\t37 7 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [115] "28\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [116] "29\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [117] "31\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [118] "32\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [119] "33\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [120] "34\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [121] "35\t3\t0.0\t3\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [122] "38\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [123] "39\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [124] "40\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [125] "41\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [126] "42\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [127] "44\t9\t0.0\t4\t5 2 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [128] "46\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [129] "47\t2\t0.0\t4\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [130] "50\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [131] "51\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [132] "52\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [133] "53\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [134] "54\t44\t0.0\t4\t38 6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [135] "55\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [136] "58\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [137] "60\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [138] "62\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [139] "63\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [140] "64\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [141] "65\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [142] "67\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [143] "70\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [144] "72\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [145] "73\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [146] "75\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [147] "78\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [148] "79\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [149] "80\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [150] "81\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [151] "83\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [152] "84\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [153] "85\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [154] "86\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [155] "89\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [156] "90\t8\t0.0\t4\t7 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [157] "92\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [158] "93\t15\t0.0\t4\t13 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [159] "94\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [160] "95\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [161] "96\t31\t0.0\t4\t31"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [162] "97\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [163] "100\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [164] "101\t43\t0.0\t4\t9 30 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [165] "102\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [166] "103\t11\t0.0\t4\t10 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [167] "104\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [168] "105\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [169] "108\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [170] "109\t14\t0.0\t4\t14"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [171] "110\t11\t0.0\t4\t11"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [172] "111\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [173] "112\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [174] "113\t9\t0.0\t4\t0 0 8 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [175] "114\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [176] "115\t5\t0.0\t4\t0 0 5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [177] "118\t136\t0.0\t4\t129 7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [178] "119\t9\t0.0\t4\t8 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [179] "120\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [180] "125\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [181] "126\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [182] "132\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [183] "134\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [184] "138\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [185] "141\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [186] "145\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [187] "147\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [188] "149\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [189] "151\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [190] "153\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [191] "154\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [192] "155\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [193] "156\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [194] "161\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [195] "168\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [196] "174\t4\t0.0\t4\t1 0 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [197] "176\t5\t0.0\t4\t0 0 4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [198] "178\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [199] "183\t2\t0.0\t4\t0 0 0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [200] "184\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [201] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [202] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [203] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [204] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [205] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## 
## [[121]]
##  [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/QWRNA2_S121_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/QWRNA2_S121_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/QWRNA2_S121_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/QWRNA2_S121_L001_R2_001.fastq.gz"
##  [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [4] "Finished in 0.02 s (240 us/read; 0.25 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [8] "Total read pairs processed:                 76"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [9] "  Read 1 with adapter:                       0 (0.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [10] "  Read 2 with adapter:                       2 (2.6%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [11] "Pairs written (passing filters):            76 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [13] "Total basepairs processed:        31,920 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [14] "  Read 1:        14,896 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [15] "  Read 2:        17,024 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [16] "Total written (filtered):         31,817 bp (99.7%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [17] "  Read 1:        14,896 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [18] "  Read 2:        16,921 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [24] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [25] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [26] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [27] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [28] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [29] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [30] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [32] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [34] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 2 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [36] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [37] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [39] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [40] "  A: 50.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [41] "  C: 50.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [42] "  G: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [43] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [44] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [45] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [46] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [47] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [48] "37\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [49] "66\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## 
## [[122]]
##  [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/QWRNA3_S122_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/QWRNA3_S122_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/QWRNA3_S122_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/QWRNA3_S122_L001_R2_001.fastq.gz"
##  [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [4] "Finished in 0.06 s (137 us/read; 0.44 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [8] "Total read pairs processed:                410"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [9] "  Read 1 with adapter:                       0 (0.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [10] "  Read 2 with adapter:                      11 (2.7%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [11] "Pairs written (passing filters):           410 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [13] "Total basepairs processed:       172,200 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [14] "  Read 1:        80,360 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [15] "  Read 2:        91,840 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [16] "Total written (filtered):        171,454 bp (99.6%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [17] "  Read 1:        80,360 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [18] "  Read 2:        91,094 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [24] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [25] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [26] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [27] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [28] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [29] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [30] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [32] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [34] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 11 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [36] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [37] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [39] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [40] "  A: 81.8%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [41] "  C: 9.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [42] "  G: 9.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [43] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [44] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [45] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [46] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [47] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [48] "8\t1\t0.0\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [49] "35\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [50] "38\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [51] "45\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [52] "58\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [53] "66\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [54] "83\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [55] "86\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [56] "93\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [57] "106\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [58] "128\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## 
## [[123]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/Rain_S123_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/Rain_S123_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/Rain_S123_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/Rain_S123_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##   [4] "Finished in 2.65 s (116 us/read; 0.52 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##   [8] "Total read pairs processed:             22,851"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##   [9] "  Read 1 with adapter:                       3 (0.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [10] "  Read 2 with adapter:                     183 (0.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [11] "Pairs written (passing filters):        22,851 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [13] "Total basepairs processed:     9,597,420 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [14] "  Read 1:     4,478,796 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [15] "  Read 2:     5,118,624 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [16] "Total written (filtered):      9,586,403 bp (99.9%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [17] "  Read 1:     4,478,736 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [18] "  Read 2:     5,107,667 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [29] "19\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [30] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [32] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [34] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 2 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [36] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [37] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [39] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [40] "  A: 100.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [41] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [42] "  G: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [43] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [44] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [45] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [46] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [47] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [48] "17\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [49] "24\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [50] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [51] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [52] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [53] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [54] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 3 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [55] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [56] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [57] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [58] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [59] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [60] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [61] "7\t1\t1.4\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [62] "12\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [63] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [64] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [65] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [66] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [67] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 180 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [68] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [69] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [70] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [71] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [72] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [73] "  A: 90.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [74] "  C: 5.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [75] "  G: 0.6%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [76] "  T: 3.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [77] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [78] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [79] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [80] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [81] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [82] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [83] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [84] "5\t7\t22.3\t0\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [85] "6\t2\t5.6\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [86] "7\t3\t1.4\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [87] "8\t5\t0.3\t0\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [88] "9\t2\t0.1\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [89] "10\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [90] "11\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [91] "12\t5\t0.0\t1\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [92] "14\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [93] "15\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [94] "16\t5\t0.0\t1\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [95] "17\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [96] "18\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [97] "20\t4\t0.0\t2\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [98] "21\t2\t0.0\t2\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [99] "22\t3\t0.0\t2\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [100] "23\t4\t0.0\t2\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [101] "24\t2\t0.0\t2\t0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [102] "26\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [103] "27\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [104] "28\t1\t0.0\t2\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [105] "31\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [106] "32\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [107] "33\t3\t0.0\t3\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [108] "34\t4\t0.0\t3\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [109] "35\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [110] "36\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [111] "37\t2\t0.0\t3\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [112] "40\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [113] "41\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [114] "42\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [115] "44\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [116] "45\t2\t0.0\t4\t0 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [117] "46\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [118] "47\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [119] "50\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [120] "51\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [121] "52\t4\t0.0\t4\t3 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [122] "53\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [123] "54\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [124] "55\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [125] "56\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [126] "57\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [127] "59\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [128] "62\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [129] "63\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [130] "64\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [131] "65\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [132] "69\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [133] "72\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [134] "73\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [135] "75\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [136] "77\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [137] "78\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [138] "79\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [139] "80\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [140] "82\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [141] "84\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [142] "85\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [143] "86\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [144] "87\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [145] "88\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [146] "89\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [147] "91\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [148] "92\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [149] "93\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [150] "96\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [151] "97\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [152] "98\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [153] "100\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [154] "101\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [155] "103\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [156] "104\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [157] "105\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [158] "106\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [159] "108\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [160] "109\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [161] "111\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [162] "115\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [163] "117\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [164] "118\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [165] "121\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [166] "122\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [167] "124\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [168] "127\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [169] "132\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [170] "134\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [171] "139\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [172] "141\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [173] "143\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [174] "144\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [175] "146\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [176] "147\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [177] "148\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [178] "151\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [179] "152\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [180] "160\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [181] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [182] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [183] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [184] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [185] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## 
## [[124]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/RainRNA_S124_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/RainRNA_S124_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/RainRNA_S124_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/RainRNA_S124_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##   [4] "Finished in 0.79 s (124 us/read; 0.48 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [8] "Total read pairs processed:              6,345"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [9] "  Read 1 with adapter:                      15 (0.2%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [10] "  Read 2 with adapter:                     359 (5.7%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [11] "Pairs written (passing filters):         6,345 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [13] "Total basepairs processed:     2,664,900 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [14] "  Read 1:     1,243,620 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [15] "  Read 2:     1,421,280 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [16] "Total written (filtered):      2,640,358 bp (99.1%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [17] "  Read 1:     1,242,995 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [18] "  Read 2:     1,397,363 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 3 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [29] "9\t2\t0.0\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [30] "10\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [32] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [33] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [34] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [35] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 12 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [36] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [37] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [38] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [39] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [40] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [41] "  A: 41.7%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [42] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [43] "  G: 58.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [44] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [45] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [46] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [47] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [48] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [49] "13\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [50] "15\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [51] "19\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [52] "28\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [53] "30\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [54] "36\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [55] "64\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [56] "73\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [57] "75\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [58] "90\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [59] "118\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [60] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [61] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [62] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [63] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [64] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [65] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [66] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [67] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [68] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 359 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [69] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [70] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [71] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [72] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [73] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [74] "  A: 88.3%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [75] "  C: 8.9%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [76] "  G: 1.4%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [77] "  T: 1.4%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [78] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [79] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [80] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [81] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [82] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [83] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [84] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [85] "5\t3\t6.2\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [86] "6\t4\t1.5\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [87] "7\t4\t0.4\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [88] "9\t2\t0.0\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [89] "10\t6\t0.0\t1\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [90] "12\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [91] "13\t7\t0.0\t1\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [92] "14\t10\t0.0\t1\t10"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [93] "15\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [94] "16\t4\t0.0\t1\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [95] "17\t6\t0.0\t1\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [96] "19\t5\t0.0\t1\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [97] "20\t11\t0.0\t2\t8 2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [98] "21\t4\t0.0\t2\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [99] "22\t4\t0.0\t2\t2 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [100] "23\t3\t0.0\t2\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [101] "24\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [102] "25\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [103] "26\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [104] "27\t2\t0.0\t2\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [105] "28\t3\t0.0\t2\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [106] "30\t3\t0.0\t3\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [107] "31\t2\t0.0\t3\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [108] "33\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [109] "34\t3\t0.0\t3\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [110] "37\t2\t0.0\t3\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [111] "38\t4\t0.0\t3\t3 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [112] "40\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [113] "41\t2\t0.0\t4\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [114] "42\t5\t0.0\t4\t3 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [115] "43\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [116] "44\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [117] "45\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [118] "46\t7\t0.0\t4\t6 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [119] "47\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [120] "48\t3\t0.0\t4\t1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [121] "49\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [122] "50\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [123] "51\t4\t0.0\t4\t2 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [124] "52\t9\t0.0\t4\t5 1 0 1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [125] "53\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [126] "55\t2\t0.0\t4\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [127] "56\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [128] "57\t3\t0.0\t4\t2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [129] "58\t8\t0.0\t4\t7 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [130] "59\t4\t0.0\t4\t2 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [131] "60\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [132] "62\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [133] "64\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [134] "65\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [135] "66\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [136] "67\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [137] "68\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [138] "69\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [139] "70\t3\t0.0\t4\t2 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [140] "71\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [141] "72\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [142] "73\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [143] "74\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [144] "75\t7\t0.0\t4\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [145] "76\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [146] "77\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [147] "78\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [148] "79\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [149] "80\t4\t0.0\t4\t2 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [150] "81\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [151] "82\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [152] "83\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [153] "84\t5\t0.0\t4\t4 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [154] "85\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [155] "86\t11\t0.0\t4\t10 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [156] "87\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [157] "88\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [158] "90\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [159] "91\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [160] "92\t6\t0.0\t4\t4 0 1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [161] "93\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [162] "94\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [163] "95\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [164] "96\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [165] "97\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [166] "98\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [167] "99\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [168] "100\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [169] "101\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [170] "102\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [171] "103\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [172] "104\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [173] "105\t7\t0.0\t4\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [174] "106\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [175] "107\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [176] "108\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [177] "109\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [178] "110\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [179] "111\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [180] "116\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [181] "117\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [182] "118\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [183] "121\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [184] "123\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [185] "125\t2\t0.0\t4\t1 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [186] "127\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [187] "129\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [188] "131\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [189] "132\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [190] "136\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [191] "137\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [192] "141\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [193] "144\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [194] "145\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [195] "146\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [196] "147\t1\t0.0\t4\t0 0 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [197] "149\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [198] "154\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [199] "156\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [200] "158\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [201] "159\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [202] "162\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [203] "163\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [204] "165\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [205] "169\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [206] "172\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [207] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [208] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [209] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [210] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [211] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## 
## [[125]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/RSawWat_S125_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/RSawWat_S125_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/RSawWat_S125_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/RSawWat_S125_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##   [4] "Finished in 2.47 s (101 us/read; 0.60 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##   [8] "Total read pairs processed:             24,524"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##   [9] "  Read 1 with adapter:                       8 (0.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [10] "  Read 2 with adapter:                     244 (1.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [11] "Pairs written (passing filters):        24,523 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [13] "Total basepairs processed:    10,300,080 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [14] "  Read 1:     4,806,704 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [15] "  Read 2:     5,493,376 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [16] "Total written (filtered):     10,284,355 bp (99.8%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [17] "  Read 1:     4,806,264 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [18] "  Read 2:     5,478,091 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 7 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [29] "7\t1\t1.5\t0\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [30] "9\t2\t0.1\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [31] "26\t1\t0.0\t2\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [32] "29\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [33] "36\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [34] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [36] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [38] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 1 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [39] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [40] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [41] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [42] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [43] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [44] "  A: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [45] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [46] "  G: 100.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [47] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [48] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [49] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [50] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [51] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [52] "92\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [53] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [54] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [55] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [56] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [57] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [58] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [59] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [60] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [61] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 244 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [62] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [63] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [64] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [65] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [66] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [67] "  A: 95.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [68] "  C: 4.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [69] "  G: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [70] "  T: 0.4%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [71] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [72] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [73] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [74] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [75] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [76] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [77] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [78] "5\t10\t23.9\t0\t10"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [79] "6\t4\t6.0\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [80] "7\t2\t1.5\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [81] "8\t4\t0.4\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [82] "9\t3\t0.1\t0\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [83] "11\t1\t0.0\t1\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [84] "12\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [85] "13\t4\t0.0\t1\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [86] "14\t4\t0.0\t1\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [87] "15\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [88] "16\t5\t0.0\t1\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [89] "17\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [90] "20\t4\t0.0\t2\t2 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [91] "21\t3\t0.0\t2\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [92] "22\t4\t0.0\t2\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [93] "23\t5\t0.0\t2\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [94] "24\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [95] "25\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [96] "28\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [97] "29\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [98] "30\t3\t0.0\t3\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [99] "31\t2\t0.0\t3\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [100] "32\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [101] "33\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [102] "34\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [103] "35\t3\t0.0\t3\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [104] "36\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [105] "39\t2\t0.0\t3\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [106] "40\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [107] "41\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [108] "44\t2\t0.0\t4\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [109] "45\t2\t0.0\t4\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [110] "46\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [111] "47\t4\t0.0\t4\t3 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [112] "49\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [113] "50\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [114] "51\t5\t0.0\t4\t3 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [115] "52\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [116] "53\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [117] "54\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [118] "55\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [119] "57\t2\t0.0\t4\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [120] "58\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [121] "59\t3\t0.0\t4\t2 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [122] "60\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [123] "61\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [124] "62\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [125] "63\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [126] "65\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [127] "66\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [128] "67\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [129] "69\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [130] "71\t1\t0.0\t4\t0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [131] "72\t4\t0.0\t4\t2 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [132] "73\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [133] "74\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [134] "75\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [135] "78\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [136] "79\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [137] "81\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [138] "82\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [139] "84\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [140] "85\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [141] "86\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [142] "87\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [143] "88\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [144] "89\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [145] "91\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [146] "92\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [147] "93\t6\t0.0\t4\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [148] "94\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [149] "95\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [150] "96\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [151] "98\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [152] "100\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [153] "101\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [154] "102\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [155] "103\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [156] "104\t6\t0.0\t4\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [157] "105\t17\t0.0\t4\t15 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [158] "106\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [159] "108\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [160] "109\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [161] "110\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [162] "111\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [163] "113\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [164] "114\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [165] "116\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [166] "118\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
## [167] "120\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [168] "123\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [169] "124\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [170] "126\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [171] "128\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [172] "130\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [173] "134\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [174] "145\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [175] "153\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [176] "154\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [177] "163\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [178] "164\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [179] "177\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [180] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [181] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [182] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [183] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [184] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## 
## [[126]]
##   [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##   [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/RSawWatRNA_S126_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/RSawWatRNA_S126_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/RSawWatRNA_S126_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/RSawWatRNA_S126_L001_R2_001.fastq.gz"
##   [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##   [4] "Finished in 1.68 s (99 us/read; 0.60 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##   [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##   [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##   [8] "Total read pairs processed:             16,970"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##   [9] "  Read 1 with adapter:                      39 (0.2%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [10] "  Read 2 with adapter:                     864 (5.1%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [11] "Pairs written (passing filters):        16,970 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [13] "Total basepairs processed:     7,127,400 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
##  [14] "  Read 1:     3,326,120 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [15] "  Read 2:     3,801,280 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [16] "Total written (filtered):      7,060,946 bp (99.1%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
##  [17] "  Read 1:     3,324,511 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [18] "  Read 2:     3,736,435 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 5 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [24] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [25] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [26] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [27] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [28] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [29] "28\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [30] "32\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [31] "33\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [32] "35\t1\t0.0\t3\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [33] "66\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [34] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [36] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [37] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [38] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 34 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [39] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [40] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [41] "0-9 bp: 0; 10-19 bp: 1; 20 bp: 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [42] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [43] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [44] "  A: 76.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [45] "  C: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [46] "  G: 23.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [47] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [48] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [49] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [50] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [51] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [52] "6\t2\t4.1\t0\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [53] "10\t2\t0.0\t1\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [54] "14\t1\t0.0\t1\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [55] "19\t10\t0.0\t1\t10"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [56] "23\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [57] "36\t3\t0.0\t2\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [58] "56\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [59] "57\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [60] "58\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [61] "66\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [62] "67\t4\t0.0\t2\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [63] "69\t5\t0.0\t2\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [64] "76\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [65] "122\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [66] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [67] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [68] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [69] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [70] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [71] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [72] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [73] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [74] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 864 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [75] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [76] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [77] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [78] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [79] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [80] "  A: 97.5%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
##  [81] "  C: 2.4%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [82] "  G: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [83] "  T: 0.1%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
##  [84] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [85] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
##  [86] "    The adapter is preceded by \"A\" extremely often."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
##  [87] "    The provided adapter sequence could be incomplete at its 3' end."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
##  [88] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [89] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
##  [90] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
##  [91] "5\t4\t16.6\t0\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [92] "6\t7\t4.1\t0\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [93] "7\t6\t1.0\t0\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [94] "8\t7\t0.3\t0\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [95] "9\t9\t0.1\t0\t9"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [96] "10\t3\t0.0\t1\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [97] "11\t3\t0.0\t1\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
##  [98] "12\t4\t0.0\t1\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [99] "13\t12\t0.0\t1\t11 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [100] "14\t6\t0.0\t1\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [101] "16\t5\t0.0\t1\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [102] "17\t3\t0.0\t1\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [103] "18\t2\t0.0\t1\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [104] "19\t5\t0.0\t1\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [105] "20\t5\t0.0\t2\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [106] "21\t3\t0.0\t2\t1 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [107] "22\t8\t0.0\t2\t6 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [108] "23\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [109] "24\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [110] "25\t6\t0.0\t2\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [111] "26\t2\t0.0\t2\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [112] "27\t6\t0.0\t2\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [113] "28\t2\t0.0\t2\t1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [114] "29\t1\t0.0\t2\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [115] "30\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [116] "31\t6\t0.0\t3\t3 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [117] "32\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [118] "33\t9\t0.0\t3\t6 1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [119] "34\t10\t0.0\t3\t8 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [120] "35\t4\t0.0\t3\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [121] "36\t1\t0.0\t3\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [122] "37\t4\t0.0\t3\t2 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [123] "38\t10\t0.0\t3\t7 1 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [124] "39\t5\t0.0\t3\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [125] "40\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [126] "41\t6\t0.0\t4\t5 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [127] "42\t7\t0.0\t4\t5 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [128] "44\t10\t0.0\t4\t9 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [129] "45\t13\t0.0\t4\t8 4 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [130] "46\t9\t0.0\t4\t7 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [131] "47\t12\t0.0\t4\t9 2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [132] "48\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [133] "49\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [134] "50\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [135] "51\t7\t0.0\t4\t6 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [136] "52\t27\t0.0\t4\t22 4 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [137] "53\t7\t0.0\t4\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [138] "54\t7\t0.0\t4\t5 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [139] "55\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [140] "56\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [141] "57\t9\t0.0\t4\t8 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [142] "58\t14\t0.0\t4\t8 3 0 3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [143] "59\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [144] "60\t7\t0.0\t4\t6 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [145] "61\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [146] "62\t5\t0.0\t4\t3 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [147] "63\t7\t0.0\t4\t4 2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [148] "64\t11\t0.0\t4\t9 1 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [149] "65\t18\t0.0\t4\t15 2 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [150] "66\t7\t0.0\t4\t6 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [151] "67\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [152] "68\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [153] "69\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [154] "71\t6\t0.0\t4\t5 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [155] "72\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [156] "73\t6\t0.0\t4\t4 1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [157] "74\t11\t0.0\t4\t10 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [158] "75\t15\t0.0\t4\t15"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [159] "76\t11\t0.0\t4\t10 0 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [160] "77\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [161] "78\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [162] "79\t13\t0.0\t4\t12 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [163] "80\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [164] "81\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [165] "82\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [166] "83\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [167] "84\t11\t0.0\t4\t10 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [168] "85\t7\t0.0\t4\t6 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [169] "86\t19\t0.0\t4\t18 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [170] "87\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [171] "88\t9\t0.0\t4\t9"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [172] "89\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [173] "90\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [174] "91\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [175] "92\t8\t0.0\t4\t6 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [176] "93\t7\t0.0\t4\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [177] "94\t23\t0.0\t4\t21 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [178] "95\t9\t0.0\t4\t8 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [179] "96\t6\t0.0\t4\t6"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [180] "97\t14\t0.0\t4\t12 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
## [181] "98\t7\t0.0\t4\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [182] "99\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [183] "100\t8\t0.0\t4\t7 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [184] "101\t5\t0.0\t4\t4 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [185] "102\t7\t0.0\t4\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [186] "103\t16\t0.0\t4\t14 2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [187] "104\t37\t0.0\t4\t31 5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [188] "105\t32\t0.0\t4\t28 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [189] "106\t8\t0.0\t4\t7 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [190] "107\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [191] "108\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [192] "109\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [193] "110\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [194] "111\t6\t0.0\t4\t5 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [195] "112\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [196] "113\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [197] "114\t5\t0.0\t4\t3 1 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [198] "115\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [199] "116\t7\t0.0\t4\t6 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [200] "117\t5\t0.0\t4\t4 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [201] "118\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [202] "119\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [203] "120\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [204] "121\t7\t0.0\t4\t6 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [205] "122\t4\t0.0\t4\t3 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [206] "123\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [207] "124\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [208] "125\t3\t0.0\t4\t2 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [209] "126\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [210] "127\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [211] "128\t7\t0.0\t4\t7"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [212] "129\t4\t0.0\t4\t3 0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [213] "130\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [214] "131\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [215] "132\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [216] "133\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [217] "134\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [218] "136\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [219] "137\t5\t0.0\t4\t5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [220] "138\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [221] "139\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [222] "140\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [223] "142\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [224] "143\t3\t0.0\t4\t3"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [225] "144\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [226] "145\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [227] "146\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [228] "148\t2\t0.0\t4\t1 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [229] "149\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [230] "150\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [231] "153\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [232] "154\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [233] "159\t2\t0.0\t4\t2"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [234] "160\t4\t0.0\t4\t4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [235] "162\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [236] "163\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [237] "164\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [238] "166\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [239] "167\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [240] "172\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [241] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [242] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [243] "WARNING:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
## [244] "    One or more of your adapter sequences may be incomplete."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [245] "    Please see the detailed output above."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## 
## [[127]]
##  [1] "This is cutadapt 2.10 with Python 3.6.5"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
##  [2] "Command line parameters: -g CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA -a AAACTYAAAKRAATTGRCGG -G CCGYCAATTYMTTTRAGTTT -A TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG --overlap 5 --minimum-length 50 -n 2 -o /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered_and_cut/RtnegC_S127_L001_R1_001.fastq.gz -p /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered_and_cut/RtnegC_S127_L001_R2_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_F/filtered/RtnegC_S127_L001_R1_001.fastq.gz /Users/melo.d/Desktop/Research/Oman_fieldwork/Samail_16S_compilation_fq_outputs/2017/02_filter/preprocessed_R/filtered/RtnegC_S127_L001_R2_001.fastq.gz"
##  [3] "Processing reads on 1 core in paired-end mode ..."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
##  [4] "Finished in 0.02 s (272 us/read; 0.22 M reads/minute)."                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
##  [5] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [6] "=== Summary ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
##  [7] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
##  [8] "Total read pairs processed:                 67"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
##  [9] "  Read 1 with adapter:                       0 (0.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [10] "  Read 2 with adapter:                       4 (6.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
## [11] "Pairs written (passing filters):            67 (100.0%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
## [12] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [13] "Total basepairs processed:        28,140 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
## [14] "  Read 1:        13,132 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [15] "  Read 2:        15,008 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [16] "Total written (filtered):         27,765 bp (98.7%)"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
## [17] "  Read 1:        13,132 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [18] "  Read 2:        14,633 bp"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [19] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [20] "=== First read: Adapter 1 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [21] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [22] "Sequence: CCGTAAAACGACGGCCAGTCCGTGYCAGCMGCCGCGGTAA; Type: regular 5'; Length: 40; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [23] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [24] "=== First read: Adapter 2 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [25] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [26] "Sequence: AAACTYAAAKRAATTGRCGG; Type: regular 3'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [27] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [28] "=== Second read: Adapter 3 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [29] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [30] "Sequence: CCGYCAATTYMTTTRAGTTT; Type: regular 5'; Length: 20; Trimmed: 0 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [31] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [32] "=== Second read: Adapter 4 ==="                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
## [33] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [34] "Sequence: TTACCGCGGCKGCTGRCACGGACTGGCCGTCGTTTTACGG; Type: regular 3'; Length: 40; Trimmed: 4 times"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [35] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [36] "No. of allowed errors:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
## [37] "0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-39 bp: 3; 40 bp: 4"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [38] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [39] "Bases preceding removed adapters:"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [40] "  A: 75.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [41] "  C: 25.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
## [42] "  G: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [43] "  T: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
## [44] "  none/other: 0.0%"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [45] ""                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [46] "Overview of removed sequences"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
## [47] "length\tcount\texpect\tmax.err\terror counts"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
## [48] "44\t1\t0.0\t4\t0 1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
## [49] "92\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
## [50] "106\t1\t0.0\t4\t1"                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
## [51] "133\t1\t0.0\t4\t0 1"

Count and display reads for cut

# count forward reads
cut_read_count_F <- countLines(filt_and_cut_path_F)/4

# make forward read counts into tbl with sample name
cut_read_count_F_tbl <- cut_read_count_F %>% as_tibble(rownames = "file_name") %>% rename(reads_F = value) %>% mutate(sample_id = sapply(strsplit(file_name, "_S[[:digit:]]"), `[`, 1))

# repeat for reverse
cut_read_count_R <- countLines(filt_and_cut_path_R)/4

cut_read_count_R_tbl <- cut_read_count_R %>% as_tibble(rownames = "file_name") %>% rename(reads_R = value) %>% mutate(sample_id = sapply(strsplit(file_name, "_S[[:digit:]]"), `[`, 1))

# Join forward and reverse together into one tbl
cut_read_count_FR <- cut_read_count_F_tbl %>% select(-file_name) %>% left_join(cut_read_count_R_tbl %>% select(-file_name), by = "sample_id") %>% select(sample_id, everything()) %>% mutate(FR_identical = identical(reads_F, reads_R)) %>% arrange(FR_identical)

# print. reads counts are same in forward and reverse, which is what we want.
cut_read_count_FR

Plot read counts

plot_read_count_cut <- cut_read_count_FR %>% ggplot(aes(
  x = fct_reorder(sample_id, desc(reads_F)),
  y = reads_F
)) +
  geom_bar(stat = "identity") +
  scale_y_continuous(name = "Forward reads") +
  scale_x_discrete(name = "Sample ID") +
  theme_classic(base_size = 9)+
  theme(
    axis.text.x = element_text(angle = 90, vjust = .5, hjust = 1),
    legend.position = "bottom"
  )

plot_read_count_cut

It looks like, so far, we’ve kept most of the reads we started with.

6.3 Check primer removal after cutadapt

# As a sanity check, we will check for primers in the first cutadapt-ed sample:
    ## should all be zero!
rbind(FWD.ForwardReads = sapply(FWD.orients, primerHits, fn = fnFs.cut[[1]]), 
      FWD.ReverseReads = sapply(FWD.orients, primerHits, fn = fnRs.cut[[1]]), 
      REV.ForwardReads = sapply(REV.orients, primerHits, fn = fnFs.cut[[1]]), 
      REV.ReverseReads = sapply(REV.orients, primerHits, fn = fnRs.cut[[1]]))
##                  Forward Complement Reverse RevComp
## FWD.ForwardReads       0          0       0       0
## FWD.ReverseReads       0          0       0       0
## REV.ForwardReads       1          0       0       0
## REV.ReverseReads       0          0       0       3
# same for the second sample
rbind(FWD.ForwardReads = sapply(FWD.orients, primerHits, fn = fnFs.cut[[2]]), 
      FWD.ReverseReads = sapply(FWD.orients, primerHits, fn = fnRs.cut[[2]]), 
      REV.ForwardReads = sapply(REV.orients, primerHits, fn = fnFs.cut[[2]]), 
      REV.ReverseReads = sapply(REV.orients, primerHits, fn = fnRs.cut[[2]]))
##                  Forward Complement Reverse RevComp
## FWD.ForwardReads       0          0       0       0
## FWD.ReverseReads       0          0       0       0
## REV.ForwardReads       0          0       0       0
## REV.ReverseReads       0          0       0       1

Primers look to be fully removed.

6.4 Plot the quality of the filtered and cut fastq files.

# Forward reads
# If the number of samples is 20 or less, plot them all, otherwise, just plot 20 randomly selected samples
if( length(fnFs.cut) <= 20) {
  plotQualityProfile(fnFs.cut)
} else {
  rand_samples <- sample(size = 20, 1:length(fnFs.cut)) # grab 20 random samples to plot
  plotQualityProfile(fnFs.cut[rand_samples])
}

# Forward reads
# If the number of samples is 20 or less, plot them all, otherwise, just plot 20 randomly selected samples
if( length(fnRs.cut) <= 20) {
  plotQualityProfile(fnRs.cut)
} else {
  rand_samples <- sample(size = 20, 1:length(fnRs.cut)) # grab 20 random samples to plot
  plotQualityProfile(fnRs.cut[rand_samples])
}

7 Infer sequence variants

In this part of the pipeline, dada2 will learn to distinguish error from biological differences using a subset of our data as a training set. After it understands the error rates, we will reduce the size of the dataset by combining all identical sequence reads into “unique sequences”. Then, using the dereplicated data and error rates, dada2 will infer the sequence variants in our data. Finally, we will merge the corresponding forward and reverse reads to create a list of the fully denoised sequences and create a sequence table from the result.

Extract sample names

# Extract sample names, assuming filenames have format: SAMPLENAME_XXX.fastq
sample.namesF_no_dots <- sapply(strsplit(basename(fnFs.cut), "_S[[:digit:]]"), `[`, 1)
sample.namesR_no_dots <- sapply(strsplit(basename(fnRs.cut), "_S[[:digit:]]"), `[`, 1)

# Double check
if(!identical(sample.namesF_no_dots, sample.namesR_no_dots)) stop("Forward and reverse files do not match.")

# Now, we don't want to add the names without dots (which were only formatted this way to make the files readable for Figaro). Rather, we want the original sample names extracted at the start of this document. Unfortunately, these are not sorted in the same order due to the punctuation change, so we need to do some data wrangling to get the names with dots in the same order.

sample.namesF.dots_tbl <- as_tibble(sample.namesF.dots) %>% rename(name_w_dots = value) %>% mutate(name_no_dots = name_w_dots %>% str_replace_all("\\.", "")) %>% arrange(name_no_dots)

sample.namesF.dots.arranged <- sample.namesF.dots_tbl$name_w_dots

if(!identical(sample.namesF_no_dots, (sample.namesF.dots.arranged %>% str_replace_all("\\.", "")))) stop("Names without dots and original names with dots extracted are different or in different orders. (forward)")

sample.namesR.dots_tbl <- as_tibble(sample.namesR.dots) %>% rename(name_w_dots = value) %>% mutate(name_no_dots = name_w_dots %>% str_replace_all("\\.", "")) %>% arrange(name_no_dots)

sample.namesR.dots.arranged <- sample.namesR.dots_tbl$name_w_dots

if(!identical(sample.namesR_no_dots, (sample.namesR.dots.arranged %>% str_replace_all("\\.", "")))) stop("Names without dots and original names with dots extracted are different or in different orders. (reverse)")

names(fnFs.cut) <- sample.namesF.dots.arranged
names(fnRs.cut) <- sample.namesR.dots.arranged

7.1 Learn the error rates

set.seed(100) # set seed to ensure that randomized steps are reproducible

# Learn forward error rates
errF <- learnErrors(fnFs.cut, nbases=5e8, multithread=TRUE)
## 198555027 total bases in 1014130 reads from 127 samples will be used for learning the error rates.
# Learn reverse error rates
errR <- learnErrors(fnRs.cut, nbases=5e8, multithread=TRUE)
## 225505765 total bases in 1014130 reads from 127 samples will be used for learning the error rates.

7.1.1 Plot Error Rates

The error rates for each possible transition (A→C, A→G, …) are shown. Points are the observed error rates for each consensus quality score. The black line shows the estimated error rates after convergence of the machine-learning algorithm. The red line shows the error rates expected under the nominal definition of the Q-score. Here, we want to see estimated error rates (black line) that are a good fit to the observed rates (points), and that the error rates drop with increased quality as expected. If these criteria aren’t observed, then it may be a good idea to increase the nbases parameter. This allows the machine learning algorithm to train on a larger portion of your data and may help improve the fit.

plotErrors(errF, nominalQ=TRUE)
## Warning: Transformation introduced infinite values in continuous y-axis

## Warning: Transformation introduced infinite values in continuous y-axis

plotErrors(errR, nominalQ=TRUE)
## Warning: Transformation introduced infinite values in continuous y-axis

## Warning: Transformation introduced infinite values in continuous y-axis

7.2 Dereplication, sequence inference, and merging of paired-end reads

# make lists to hold the loop output
mergers <- vector("list", length(sample.namesF.dots.arranged))
names(mergers) <- sample.namesF.dots.arranged
ddF <- vector("list", length(sample.namesF.dots.arranged))
names(ddF) <- sample.namesF.dots.arranged
ddR <- vector("list", length(sample.namesF.dots.arranged))
names(ddR) <- sample.namesF.dots.arranged

# For each sample, get a list of merged and denoised sequences
for(sam in sample.namesF.dots.arranged) {
    cat("Processing:", sam, "\n")
    # Dereplicate forward reads
    derepF <- derepFastq(fnFs.cut[[sam]])
    # Infer sequences for forward reads
    ddF[[sam]] <- dada(derepF, err=errF, multithread=TRUE)
    # Dereplicate reverse reads
    derepR <- derepFastq(fnRs.cut[[sam]])
    # Infer sequences for reverse reads
    ddR[[sam]] <- dada(derepR, err=errR, multithread=TRUE)
    # Merge reads together
    merger <- mergePairs(ddF[[sam]], derepF, ddR[[sam]], derepR)
    mergers[[sam]] <- merger
}
## Processing: 04.RNA1 
## Sample 1 - 5910 reads in 1940 unique sequences.
## Sample 1 - 5910 reads in 3620 unique sequences.
## Processing: 04.RNA2 
## Sample 1 - 2847 reads in 1081 unique sequences.
## Sample 1 - 2847 reads in 1947 unique sequences.
## Processing: 104.1 
## Sample 1 - 9515 reads in 4085 unique sequences.
## Sample 1 - 9515 reads in 7152 unique sequences.
## Processing: 104.2 
## Sample 1 - 15301 reads in 6564 unique sequences.
## Sample 1 - 15301 reads in 11154 unique sequences.
## Processing: 104.3 
## Sample 1 - 16700 reads in 6812 unique sequences.
## Sample 1 - 16700 reads in 11858 unique sequences.
## Processing: 104.RNA1 
## Sample 1 - 4719 reads in 1909 unique sequences.
## Sample 1 - 4719 reads in 3682 unique sequences.
## Processing: 104.RNA2 
## Sample 1 - 12359 reads in 5000 unique sequences.
## Sample 1 - 12359 reads in 9582 unique sequences.
## Processing: 104.RNA3 
## Sample 1 - 14346 reads in 5112 unique sequences.
## Sample 1 - 14346 reads in 10552 unique sequences.
## Processing: 105.1 
## Sample 1 - 14658 reads in 5324 unique sequences.
## Sample 1 - 14658 reads in 10034 unique sequences.
## Processing: 105.2 
## Sample 1 - 13742 reads in 4765 unique sequences.
## Sample 1 - 13742 reads in 10023 unique sequences.
## Processing: 105.3 
## Sample 1 - 10965 reads in 3777 unique sequences.
## Sample 1 - 10965 reads in 7684 unique sequences.
## Processing: 105.RNA1 
## Sample 1 - 6497 reads in 1516 unique sequences.
## Sample 1 - 6497 reads in 4163 unique sequences.
## Processing: 105.RNA2 
## Sample 1 - 7753 reads in 1572 unique sequences.
## Sample 1 - 7753 reads in 4833 unique sequences.
## Processing: 105.RNA3 
## Sample 1 - 65 reads in 63 unique sequences.
## Sample 1 - 65 reads in 64 unique sequences.
## Processing: 14B.1 
## Sample 1 - 41425 reads in 10800 unique sequences.
## Sample 1 - 41425 reads in 24977 unique sequences.
## Processing: 14B.2 
## Sample 1 - 29472 reads in 7343 unique sequences.
## Sample 1 - 29472 reads in 16390 unique sequences.
## Processing: 14B.3 
## Sample 1 - 29025 reads in 7072 unique sequences.
## Sample 1 - 29025 reads in 15982 unique sequences.
## Processing: 14B.4 
## Sample 1 - 15609 reads in 5208 unique sequences.
## Sample 1 - 15609 reads in 10155 unique sequences.
## Processing: 14B.RNA1 
## Sample 1 - 7867 reads in 2362 unique sequences.
## Sample 1 - 7867 reads in 5107 unique sequences.
## Processing: 14B.RNA2 
## Sample 1 - 8219 reads in 2621 unique sequences.
## Sample 1 - 8219 reads in 4903 unique sequences.
## Processing: 14B.RNA3 
## Sample 1 - 8663 reads in 2481 unique sequences.
## Sample 1 - 8663 reads in 5113 unique sequences.
## Processing: 14B.RNA4 
## Sample 1 - 4119 reads in 1298 unique sequences.
## Sample 1 - 4119 reads in 2579 unique sequences.
## Processing: 14C.1 
## Sample 1 - 11911 reads in 3825 unique sequences.
## Sample 1 - 11911 reads in 8131 unique sequences.
## Processing: 14C.2 
## Sample 1 - 14079 reads in 3927 unique sequences.
## Sample 1 - 14079 reads in 8488 unique sequences.
## Processing: 14C.3 
## Sample 1 - 9282 reads in 3185 unique sequences.
## Sample 1 - 9282 reads in 6324 unique sequences.
## Processing: 14C.RNA1 
## Sample 1 - 5310 reads in 1428 unique sequences.
## Sample 1 - 5310 reads in 2673 unique sequences.
## Processing: 14C.RNA2 
## Sample 1 - 6676 reads in 2125 unique sequences.
## Sample 1 - 6676 reads in 4272 unique sequences.
## Processing: 14C.RNA3 
## Sample 1 - 4749 reads in 1272 unique sequences.
## Sample 1 - 4749 reads in 2635 unique sequences.
## Processing: 188.1 
## Sample 1 - 6964 reads in 3661 unique sequences.
## Sample 1 - 6964 reads in 5087 unique sequences.
## Processing: 188.2 
## Sample 1 - 5921 reads in 3272 unique sequences.
## Sample 1 - 5921 reads in 4463 unique sequences.
## Processing: 188.3 
## Sample 1 - 9767 reads in 5160 unique sequences.
## Sample 1 - 9767 reads in 7122 unique sequences.
## Processing: 188.4 
## Sample 1 - 17599 reads in 8166 unique sequences.
## Sample 1 - 17599 reads in 11999 unique sequences.
## Processing: 188.5 
## Sample 1 - 14416 reads in 6956 unique sequences.
## Sample 1 - 14416 reads in 10127 unique sequences.
## Processing: 188.6 
## Sample 1 - 19137 reads in 8844 unique sequences.
## Sample 1 - 19137 reads in 13155 unique sequences.
## Processing: 188.7 
## Sample 1 - 17213 reads in 8555 unique sequences.
## Sample 1 - 17213 reads in 11974 unique sequences.
## Processing: 188.RNA1 
## Sample 1 - 2776 reads in 1485 unique sequences.
## Sample 1 - 2776 reads in 2086 unique sequences.
## Processing: 188.RNA2 
## Sample 1 - 3176 reads in 1547 unique sequences.
## Sample 1 - 3176 reads in 2279 unique sequences.
## Processing: 188.RNA3 
## Sample 1 - 3880 reads in 2162 unique sequences.
## Sample 1 - 3880 reads in 2942 unique sequences.
## Processing: 188.RNA4 
## Sample 1 - 4142 reads in 2254 unique sequences.
## Sample 1 - 4142 reads in 3295 unique sequences.
## Processing: 188.RNA5 
## Sample 1 - 5003 reads in 2863 unique sequences.
## Sample 1 - 5003 reads in 3828 unique sequences.
## Processing: 188.RNA6 
## Sample 1 - 10267 reads in 4390 unique sequences.
## Sample 1 - 10267 reads in 6596 unique sequences.
## Processing: 188.RNA7 
## Sample 1 - 10772 reads in 4830 unique sequences.
## Sample 1 - 10772 reads in 7393 unique sequences.
## Processing: 4.1 
## Sample 1 - 8960 reads in 3884 unique sequences.
## Sample 1 - 8960 reads in 5804 unique sequences.
## Processing: 4.2 
## Sample 1 - 11867 reads in 4920 unique sequences.
## Sample 1 - 11867 reads in 7865 unique sequences.
## Processing: 55.1 
## Sample 1 - 11417 reads in 4503 unique sequences.
## Sample 1 - 11417 reads in 7641 unique sequences.
## Processing: 55.2 
## Sample 1 - 13753 reads in 5424 unique sequences.
## Sample 1 - 13753 reads in 9415 unique sequences.
## Processing: 55.3 
## Sample 1 - 12253 reads in 5111 unique sequences.
## Sample 1 - 12253 reads in 8134 unique sequences.
## Processing: 55.4 
## Sample 1 - 12964 reads in 5241 unique sequences.
## Sample 1 - 12964 reads in 8680 unique sequences.
## Processing: 55.RNA1 
## Sample 1 - 7834 reads in 2785 unique sequences.
## Sample 1 - 7834 reads in 5628 unique sequences.
## Processing: 55.RNA2 
## Sample 1 - 9385 reads in 2868 unique sequences.
## Sample 1 - 9385 reads in 6382 unique sequences.
## Processing: 55.RNA3 
## Sample 1 - 7776 reads in 2686 unique sequences.
## Sample 1 - 7776 reads in 5467 unique sequences.
## Processing: 55.RNA4 
## Sample 1 - 2164 reads in 902 unique sequences.
## Sample 1 - 2164 reads in 1557 unique sequences.
## Processing: 71A.1 
## Sample 1 - 17921 reads in 5177 unique sequences.
## Sample 1 - 17921 reads in 10035 unique sequences.
## Processing: 71A.2 
## Sample 1 - 20846 reads in 6131 unique sequences.
## Sample 1 - 20846 reads in 11707 unique sequences.
## Processing: 71A.3 
## Sample 1 - 16249 reads in 5026 unique sequences.
## Sample 1 - 16249 reads in 9403 unique sequences.
## Processing: 71A.RNA1 
## Sample 1 - 7100 reads in 2198 unique sequences.
## Sample 1 - 7100 reads in 4883 unique sequences.
## Processing: 71A.RNA2 
## Sample 1 - 1793 reads in 651 unique sequences.
## Sample 1 - 1793 reads in 1264 unique sequences.
## Processing: 71A.RNA3 
## Sample 1 - 208 reads in 133 unique sequences.
## Sample 1 - 208 reads in 180 unique sequences.
## Processing: 71B.1 
## Sample 1 - 16557 reads in 5892 unique sequences.
## Sample 1 - 16557 reads in 10090 unique sequences.
## Processing: 71B.RNA4 
## Sample 1 - 1148 reads in 592 unique sequences.
## Sample 1 - 1148 reads in 862 unique sequences.
## Processing: BA1.1 
## Sample 1 - 281 reads in 144 unique sequences.
## Sample 1 - 281 reads in 191 unique sequences.
## Processing: BA1.2 
## Sample 1 - 793 reads in 372 unique sequences.
## Sample 1 - 793 reads in 553 unique sequences.
## Processing: BA1.3 
## Sample 1 - 1908 reads in 837 unique sequences.
## Sample 1 - 1908 reads in 1331 unique sequences.
## Processing: BA1.DF1 
## Sample 1 - 24379 reads in 6347 unique sequences.
## Sample 1 - 24379 reads in 18009 unique sequences.
## Processing: BA1.DF2 
## Sample 1 - 11111 reads in 3358 unique sequences.
## Sample 1 - 11111 reads in 8791 unique sequences.
## Processing: BA1.DF.RNA1 
## Sample 1 - 17044 reads in 3839 unique sequences.
## Sample 1 - 17044 reads in 9214 unique sequences.
## Processing: BA1.DF.RNA2 
## Sample 1 - 9062 reads in 2469 unique sequences.
## Sample 1 - 9062 reads in 5886 unique sequences.
## Processing: BA1.RNA1 
## Sample 1 - 189 reads in 94 unique sequences.
## Sample 1 - 189 reads in 118 unique sequences.
## Processing: BA1.RNA2 
## Sample 1 - 603 reads in 373 unique sequences.
## Sample 1 - 603 reads in 496 unique sequences.
## Processing: BA1.RNA3 
## Sample 1 - 2235 reads in 1061 unique sequences.
## Sample 1 - 2235 reads in 1667 unique sequences.
## Processing: BT1B.1 
## Sample 1 - 751 reads in 378 unique sequences.
## Sample 1 - 751 reads in 616 unique sequences.
## Processing: BT1B.2 
## Sample 1 - 148 reads in 112 unique sequences.
## Sample 1 - 148 reads in 135 unique sequences.
## Processing: BT1B.3 
## Sample 1 - 132 reads in 125 unique sequences.
## Sample 1 - 132 reads in 129 unique sequences.
## Processing: BT1BCont.1 
## Sample 1 - 64 reads in 58 unique sequences.
## Sample 1 - 64 reads in 62 unique sequences.
## Processing: BT1BCont.2 
## Sample 1 - 66 reads in 59 unique sequences.
## Sample 1 - 66 reads in 66 unique sequences.
## Processing: BT1BCont.3 
## Sample 1 - 60 reads in 57 unique sequences.
## Sample 1 - 60 reads in 58 unique sequences.
## Processing: BT1BCont.RNA1 
## Sample 1 - 197 reads in 172 unique sequences.
## Sample 1 - 197 reads in 186 unique sequences.
## Processing: BT1BCont.RNA2 
## Sample 1 - 111 reads in 100 unique sequences.
## Sample 1 - 111 reads in 109 unique sequences.
## Processing: BT1BCont.RNA3 
## Sample 1 - 563 reads in 466 unique sequences.
## Sample 1 - 563 reads in 532 unique sequences.
## Processing: BT1B.RNA1 
## Sample 1 - 7701 reads in 3714 unique sequences.
## Sample 1 - 7701 reads in 5919 unique sequences.
## Processing: BT1B.RNA2 
## Sample 1 - 705 reads in 518 unique sequences.
## Sample 1 - 705 reads in 645 unique sequences.
## Processing: BT1B.RNA3 
## Sample 1 - 5171 reads in 2273 unique sequences.
## Sample 1 - 5171 reads in 3769 unique sequences.
## Processing: ExtC.1 
## Sample 1 - 1945 reads in 1111 unique sequences.
## Sample 1 - 1945 reads in 1474 unique sequences.
## Processing: ExtC.2 
## Sample 1 - 1307 reads in 716 unique sequences.
## Sample 1 - 1307 reads in 970 unique sequences.
## Processing: ExtC.3 
## Sample 1 - 2266 reads in 1234 unique sequences.
## Sample 1 - 2266 reads in 1749 unique sequences.
## Processing: ExtC.4 
## Sample 1 - 5074 reads in 2240 unique sequences.
## Sample 1 - 5074 reads in 3869 unique sequences.
## Processing: ExtC.5 
## Sample 1 - 11476 reads in 4728 unique sequences.
## Sample 1 - 11476 reads in 8392 unique sequences.
## Processing: ExtC.6 
## Sample 1 - 6024 reads in 2518 unique sequences.
## Sample 1 - 6024 reads in 3884 unique sequences.
## Processing: ExtC.RNA1 
## Sample 1 - 129 reads in 118 unique sequences.
## Sample 1 - 129 reads in 127 unique sequences.
## Processing: ExtC.RNA2 
## Sample 1 - 6230 reads in 1055 unique sequences.
## Sample 1 - 6230 reads in 2240 unique sequences.
## Processing: ExtC.RNA3 
## Sample 1 - 6780 reads in 3114 unique sequences.
## Sample 1 - 6780 reads in 4894 unique sequences.
## Processing: ExtC.RNA4 
## Sample 1 - 3551 reads in 1833 unique sequences.
## Sample 1 - 3551 reads in 2607 unique sequences.
## Processing: ExtC.RNA5 
## Sample 1 - 4467 reads in 1971 unique sequences.
## Sample 1 - 4467 reads in 3241 unique sequences.
## Processing: ExtC.RNA6 
## Sample 1 - 8321 reads in 3738 unique sequences.
## Sample 1 - 8321 reads in 5865 unique sequences.
## Processing: GT3.1 
## Sample 1 - 95 reads in 75 unique sequences.
## Sample 1 - 95 reads in 88 unique sequences.
## Processing: GT3.2 
## Sample 1 - 4025 reads in 1905 unique sequences.
## Sample 1 - 4025 reads in 3030 unique sequences.
## Processing: GT3.3 
## Sample 1 - 964 reads in 531 unique sequences.
## Sample 1 - 964 reads in 763 unique sequences.
## Processing: GT3Cont.1 
## Sample 1 - 138 reads in 118 unique sequences.
## Sample 1 - 138 reads in 133 unique sequences.
## Processing: GT3Cont.2 
## Sample 1 - 468 reads in 325 unique sequences.
## Sample 1 - 468 reads in 397 unique sequences.
## Processing: GT3Cont.3 
## Sample 1 - 347 reads in 231 unique sequences.
## Sample 1 - 347 reads in 292 unique sequences.
## Processing: GT3Cont.RNA1 
## Sample 1 - 677 reads in 602 unique sequences.
## Sample 1 - 677 reads in 662 unique sequences.
## Processing: GT3Cont.RNA2 
## Sample 1 - 122 reads in 115 unique sequences.
## Sample 1 - 122 reads in 121 unique sequences.
## Processing: GT3Cont.RNA3 
## Sample 1 - 9352 reads in 3068 unique sequences.
## Sample 1 - 9352 reads in 4809 unique sequences.
## Processing: GT3.RNA1 
## Sample 1 - 109 reads in 92 unique sequences.
## Sample 1 - 109 reads in 104 unique sequences.
## Processing: GT3.RNA2 
## Sample 1 - 695 reads in 450 unique sequences.
## Sample 1 - 695 reads in 590 unique sequences.
## Processing: GT3.RNA3 
## Sample 1 - 1531 reads in 1098 unique sequences.
## Sample 1 - 1531 reads in 1294 unique sequences.
## Processing: Inhib.C 
## Sample 1 - 9436 reads in 3608 unique sequences.
## Sample 1 - 9436 reads in 6478 unique sequences.
## Processing: Mmix.C 
## Sample 1 - 4260 reads in 1760 unique sequences.
## Sample 1 - 4260 reads in 3138 unique sequences.
## Processing: N.PCR.C 
## Sample 1 - 195 reads in 153 unique sequences.
## Sample 1 - 195 reads in 183 unique sequences.
## Processing: P.PCR.C 
## Sample 1 - 7423 reads in 2908 unique sequences.
## Sample 1 - 7423 reads in 5355 unique sequences.
## Processing: QB.1 
## Sample 1 - 13764 reads in 5840 unique sequences.
## Sample 1 - 13764 reads in 9152 unique sequences.
## Processing: QB.2 
## Sample 1 - 20333 reads in 8259 unique sequences.
## Sample 1 - 20333 reads in 13394 unique sequences.
## Processing: QB.3 
## Sample 1 - 16350 reads in 6889 unique sequences.
## Sample 1 - 16350 reads in 11000 unique sequences.
## Processing: QB.RNA1 
## Sample 1 - 1634 reads in 785 unique sequences.
## Sample 1 - 1634 reads in 1140 unique sequences.
## Processing: QB.RNA2 
## Sample 1 - 872 reads in 493 unique sequences.
## Sample 1 - 872 reads in 646 unique sequences.
## Processing: QB.RNA3 
## Sample 1 - 645 reads in 478 unique sequences.
## Sample 1 - 645 reads in 586 unique sequences.
## Processing: QW.1 
## Sample 1 - 20337 reads in 8171 unique sequences.
## Sample 1 - 20337 reads in 12789 unique sequences.
## Processing: QW.2 
## Sample 1 - 18861 reads in 7141 unique sequences.
## Sample 1 - 18861 reads in 11491 unique sequences.
## Processing: QW.3 
## Sample 1 - 14379 reads in 5475 unique sequences.
## Sample 1 - 14379 reads in 8845 unique sequences.
## Processing: QW.RNA1 
## Sample 1 - 12021 reads in 4502 unique sequences.
## Sample 1 - 12021 reads in 7999 unique sequences.
## Processing: QW.RNA2 
## Sample 1 - 76 reads in 56 unique sequences.
## Sample 1 - 76 reads in 73 unique sequences.
## Processing: QW.RNA3 
## Sample 1 - 410 reads in 221 unique sequences.
## Sample 1 - 410 reads in 335 unique sequences.
## Processing: Rain 
## Sample 1 - 22851 reads in 7938 unique sequences.
## Sample 1 - 22851 reads in 15587 unique sequences.
## Processing: Rain.RNA 
## Sample 1 - 6345 reads in 2968 unique sequences.
## Sample 1 - 6345 reads in 4955 unique sequences.
## Processing: RSawWat 
## Sample 1 - 24523 reads in 6568 unique sequences.
## Sample 1 - 24523 reads in 13847 unique sequences.
## Processing: RSawWat.RNA 
## Sample 1 - 16970 reads in 3494 unique sequences.
## Sample 1 - 16970 reads in 6925 unique sequences.
## Processing: Rtneg.C 
## Sample 1 - 67 reads in 67 unique sequences.
## Sample 1 - 67 reads in 66 unique sequences.
rm(derepF); rm(derepR)

7.3 Construct sequence table

seqtab <- makeSequenceTable(mergers)
write_rds(seqtab, path = format(Sys.Date(), "data_output/seqtab_OM17_processed_%Y%m%d_3.rds"), compress = "gz")

8 Remove Chimeras

Although dada2 has searched for insertion/deletion errors and substitutions, there may still be chimeric sequences in our dataset (sequences that are derived from forward and reverse sequences from two different organisms becoming fused together during PCR and/or sequencing). To identify chimeras, we will search for rare sequence variants that can be reconstructed by combining left-hand and right-hand segments from two more abundant “parent” sequences.

# Remove chimeras
seqtab_nochim <- removeBimeraDenovo(seqtab, method="consensus", multithread=TRUE)
# Print percentage of our sequences that were not chimeric.
100*sum(seqtab_nochim)/sum(seqtab)
## [1] 96.49455
write_rds(seqtab_nochim, path = format(Sys.Date(), "data_output/seqtab_nochim_OM17_processed_%Y%m%d_3.rds"), compress = "gz")

9 Track reads through pipeline

getN <- function(x) sum(getUniques(x)) # function to grab sequence counts from output objects
# tracking reads by counts
filt_out_track <- filt_out %>%
  data.frame() %>%
  mutate(Sample = gsub("(R1\\_)(.{1,})(\\.fastq\\.gz)","\\2",rownames(.))) %>%
  rename(input = reads.in, filtered = reads.out)
rownames(filt_out_track) <- filt_out_track$Sample

# have to do a few extra string manipulations in this case because of the various sample name changes for Figaro
filt_out_track <- filt_out_track %>% mutate(name_no_dots = sapply(strsplit(Sample, "_S[[:digit:]]"), `[`, 1))

filt_out_track <- filt_out_track %>% left_join(sample.namesF.dots_tbl, by = "name_no_dots")

filt_out_track <- filt_out_track %>% select(-Sample, -name_no_dots) %>% rename(Sample = name_w_dots)

# cutadapted samples
cut_read_count_FR_track <- cut_read_count_FR %>% rename(name_no_dots = sample_id) %>% left_join(sample.namesF.dots_tbl, by = "name_no_dots") %>%  select(-name_no_dots, -FR_identical, -reads_R) %>% rename(Sample = name_w_dots, cut = reads_F)

ddF_track <- data.frame(denoisedF = sapply(ddF[sample.namesF.dots.arranged], getN)) %>%
  mutate(Sample = row.names(.))
ddR_track <- data.frame(denoisedR = sapply(ddR[sample.namesF.dots.arranged], getN)) %>%
  mutate(Sample = row.names(.))
merge_track <- data.frame(merged = sapply(mergers, getN)) %>%
  mutate(Sample = row.names(.))
chim_track <- data.frame(nonchim = rowSums(seqtab_nochim)) %>%
  mutate(Sample = row.names(.))

track <- left_join(filt_out_track, cut_read_count_FR_track) %>%
  left_join(ddF_track, by = "Sample") %>%
  left_join(ddR_track, by = "Sample") %>%
  left_join(merge_track, by = "Sample") %>%
  left_join(chim_track, by = "Sample") %>%
  replace(., is.na(.), 0) %>%
  select(Sample, everything())
## Joining, by = "Sample"
row.names(track) <- track$Sample
head(track)
# tracking reads by percentage
track_pct <- track %>% 
  data.frame() %>%
  mutate(Sample = rownames(.),
         filtered_pct = ifelse(filtered == 0, 0, 100 * (filtered/input)),
          cut_pct = ifelse(cut == 0, 0, 100 * (cut/filtered)),
         denoisedF_pct = ifelse(denoisedF == 0, 0, 100 * (denoisedF/cut)),
         denoisedR_pct = ifelse(denoisedR == 0, 0, 100 * (denoisedR/cut)),
         merged_pct = ifelse(merged == 0, 0, 100 * merged/((denoisedF + denoisedR)/2)),
         nonchim_pct = ifelse(nonchim == 0, 0, 100 * (nonchim/merged)),
         total_pct = ifelse(nonchim == 0, 0, 100 * nonchim/input)) %>%
  select(Sample, ends_with("_pct"))

# summary stats of tracked reads averaged across samples
track_pct_avg <- track_pct %>% summarize_at(vars(ends_with("_pct")), 
                                            list(avg = mean))
head(track_pct_avg)
track_pct_med <- track_pct %>% summarize_at(vars(ends_with("_pct")), 
                                            list(avg = stats::median))
head(track_pct_avg)
head(track_pct_med)
# Plotting each sample's reads through the pipeline
track_plot <- track %>% 
  pivot_longer(-Sample, names_to = "Step", values_to = "Reads") %>% 
  mutate(Step = factor(Step, 
                       levels = c("input", "filtered", "cut", "denoisedF", "denoisedR", "merged", "nonchim"))) %>%
  ggplot(aes(x = Step, y = Reads)) +
  geom_line(aes(group = Sample), alpha = 0.2) +
  geom_point(alpha = 0.5, position = position_jitter(width = 0)) + 
  stat_summary(fun.y = median, geom = "line", group = 1, color = "steelblue", size = 1, alpha = 0.5) +
  stat_summary(fun.y = median, geom = "point", group = 1, color = "steelblue", size = 2, alpha = 0.5) +
  stat_summary(fun.data = median_hilow, fun.args = list(conf.int = 0.5), 
               geom = "ribbon", group = 1, fill = "steelblue", alpha = 0.2) +
  geom_label(data = t(track_pct_avg[1:6]) %>% data.frame() %>% 
               rename(Percent = 1) %>%
               mutate(Step = c("filtered", "cut", "denoisedF", "denoisedR", "merged", "nonchim"),
                      Percent = paste(round(Percent, 2), "%")),
             aes(label = Percent), y = 1.1 * max(track[,2])) +
  geom_label(data = track_pct_avg[7] %>% data.frame() %>%
               rename(total = 1),
             aes(label = paste("Total\nRemaining:\n", round(track_pct_avg[1,7], 2), "%")), 
             y = mean(track[,7]), x = 7.6) +
  expand_limits(y = 1.1 * max(track[,2]), x = 8.2) +
  theme_classic()
## Warning: `fun.y` is deprecated. Use `fun` instead.

## Warning: `fun.y` is deprecated. Use `fun` instead.
track_plot